FlyBase Aberration Report: Dmel\Df(2L)al

General Information

Symbol

Df(2L)al

Species

D. melanogaster

Name

Deficiency (2L) aristaless

FlyBase ID

FBab0001692

Feature type

chromosomal_deletion

Also Known As

Df(2)al

Computed Breakpoints include

21C1;21C7

Features within 21C1--21C7

Genomic Maps

JBrowse

Sequence coordinates

2L:300,574..303,911 [+] (Df(2L)al:bk1)

(Weinkove et al., 1997)

2L:300,000..306,000 (Df(2L)al:bk1)

(Cook, 2016.2.8)

2L:476,220..540,599 (Df(2L)al:bk2)

(Cook, 2016.2.8)

Member of large scale dataset(s)

Nature of Aberration

Cytological Order

Progenitor

Mutagen

X ray

(Lewis, 1945)

Class of aberration (relative to wild type)

chromosomal_deletion

(FlyBase, 2007)

Class of aberration (relative to progenitor)

chromosomal_deletion

(Adler et al., 1998, Dornan et al., 1997, Chandrashekaran, 1995, Harbecke and Lengyel, 1995, Pauli et al., 1995, de Belle et al., 1989, Huettner and Bryant, 1988, Korochkina and Golubovsky, 1978, Lewis, 1945)

Breakpoints

21B8-21C1;21C8-21D1

(Adler et al., 1998, Chandrashekaran, 1995, Harbecke and Lengyel, 1995, Pauli et al., 1995, de Belle et al., 1989, Lewis, 1945)

21C1;21C8

(Korochkina and Golubovsky, 1978)

21B8-21C1;21C7-21C8

(Huettner and Bryant, 1988)

21B7--21B8;[]

(Alcedo et al., 1996)

21C1;21C7

(Dornan et al., 1997)

Causes alleles

Carries alleles

ds^al

(Couso, 2000.2.10)

Transposon Insertions

Formalized genetic data

U2af38 << bk1 << fs(2)al << bk2 << lwr

Genetic mapping information

Comments

The 2L:300000..306000 release 6 coordinates of the left breakpoint are estimates based on Figure 2 of FBrf0093782.

(Cook, 2016.2.8)

The 2L:476220..540599 release 6 coordinates of the right breakpoint are estimates. The left extent corresponds to the left end of ush, which published results say is deleted. The right extent corresponds to the left end of lwr, which published results say is not deleted.

(Cook, 2016.2.8)

This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).

(Marygold et al., 2007)

Breakpoint 1 was linked to an EcoRI-EcoRI restriction fragment. The position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.

(Weinkove et al., 1997)

Breakpoint(s) molecularly mapped

(Schneitz et al., 1993)

Comments on Cytology

The distal breakpoint of Df(2L)al is about 300kb from the telomere.

(Brentrup et al., 2000)

Pi3K21B probably overlaps the distal breakpoint.

(Weinkove et al., 1997)

Left breakpoint is just before 21C1-21C2, right breakpoint is just before 21D1-21D2.

(Lewis, 1945)

Left limit of break 1 from polytene analysis (FBrf0031322) Right limit of break 1 from inclusion of al (FBrf0006343) Left limit of break 2 from polytene analysis (FBrf0047732) Right limit of break 2 from polytene analysis (FBrf0098232)

Sequence Crossreferences

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Gene Deletion and Duplication Data

Genes Deleted / Disrupted

Complementation Data

Completely deleted / disrupted

(Franc et al., 1999, Schneitz and Noll, 1990, Korochkina and Golubovsky, 1978, Lewis, 1945)

AP-2α

(Berdnik et al., 2002, Spradling et al., 1999, Gonzalez-Gaitan and Jackle, 1997, BDGP Project Members, 1994-1999)

crq

(Franc et al., 1999)

(BDGP Project Members, 1994-1999)

ebi

(Marygold et al., 2011, Dong et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

(Tyler et al., 2007, Franc et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999, Huettner and Bryant, 1988, Korochkina and Golubovsky, 1978, Lewis, 1945)

fs(2)al

(Chandrashekaran, 1995)

mbm

(Albert et al., 1995, Albert et al., 1993, Heisenberg et al., 1985)

oro

(Epps et al., 1997)

Polr1B

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

RpLP1

(Fauvarque et al., 2001, Lewis, 1945)

ush

(Franc et al., 1999, Goldman-Levi et al., 1996, Harbecke and Lengyel, 1995, Tearle and Nusslein-Volhard, 1987)

Partially deleted / disrupted

Molecular Data

Completely deleted

RpLP1

(Marygold et al., 2007)

Partially deleted

Genes NOT Deleted / Disrupted

Complementation Data

cpb

(Delalle et al., 2005)

(Couso, 2000.2.10)

for

(de Belle et al., 1989)

Gsc

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

ifm(2)RU5

(Nongthomba and Ramachandra, 1999)

kis

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(2)gl

(Brumby et al., 2004, Korochkina and Golubovsky, 1978, Lewis, 1945)

l(2)k06506

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(2)k07005

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(2)k07308

(BDGP Project Members, 1994-1999)

lwr

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

net

(Korochkina and Golubovsky, 1978, Lewis, 1945)

Nle

(Spradling et al., 1999, Royet et al., 1998, BDGP Project Members, 1994-1999)

numb

(BDGP Project Members, 1994-1999)

Pph13

(Zelhof et al., 2003)

rempA

(Lee et al., 2008)

(Lewis, 1945)

smo

(Alcedo et al., 1996, van den Heuvel and Ingham, 1996)

spen

(Eun et al., 2007)

Stip1

(BDGP Project Members, 1994-1999)

U2af38

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Molecular Data

Genes Duplicated

Complementation Data

Completely duplicated

Partially duplicated

Molecular Data

Completely duplicated

Partially duplicated

Genes NOT Duplicated

Complementation Data

Molecular Data

Affected Genes Inferred by Location

Related Comments

Stated to delete or disrupt ds. Aspects of this conclusion subsequently challenged, see FBrf0125086.

(Spradling et al., 1999, Adler et al., 1998, Korochkina and Golubovsky, 1978, Lewis, 1945)

Phenotypic Data

In combination with other aberrations

Df(2L)al dominantly enhances the transformation of arista to leg phenotype seen in Df(2R)Dll-MP heterozygotes.

(Fauvarque et al., 2001)

No effect on In(1)w^m4h position-effect variegation.

(Wustmann et al., 1989)

Viable in combination with In(2LR)al^v.

(Lewis, 1945)

Df(2L)al/In(2LR)al⁸ heterozygotes survive poorly; escapers have reduced aristae, broad thoraces, arched wings, incomplete veins and large eyes (Koroshkina and Golubovsky, 1978).

NOT in combination with other aberrations

Flies heterozygous for the deletion show a Minute bristle phenotype.

(Marygold et al., 2007)

The Df(2L)al chromosome acts as a dominant weak suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion).

(Mason et al., 2004)

Heterozygosity for Df(2L)al results in 2.6% X chromosome nondisjunction and 0.0% fourth chromosome nondisjunction in In(1)FM7/X ; sv^spa-pol females.

(Harris et al., 2003)

Wild-type clones made in the ovaries of a mutant female, are significantly larger than clones of heterozygous mutant cells. This size difference is greater in clones initiated at 48 hours after egg laying (AEL) than 2 hours AEL.

(Extavour and Garcia-Bellido, 2001)

Homozygous Df(2L)al mutants show an increase in cardial and pericardial cells during embryonic development.

(Fossett et al., 2000)

Df(2L)al in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D Dsim\Int(2L)D produces fertile male flies. These flies are female sterile.

(Sawamura et al., 2000)

Macrophages in homozygotes remain very small and round with a much lower phagocytic index (PI) for apoptotic corpses than wild type (0.26 compared to 3.96). Heterozygotes show a PI of about 1.5.

(Franc et al., 1999)

No second site non-complementing phenotype with zip^Ebr and zip^mhc-c6.1.

(Halsell and Kiehart, 1998)

Shows no maternal enhancement of dpp^hr4.

(Nicholls and Gelbart, 1998)

Does not act as a dominant suppressor of N^nd-1.

(Royet et al., 1998)

Strong enhancer of B.

(Epps et al., 1997)

Midgut development of mutant embryos is wild type.

(Bilder and Scott, 1995)

Maternal hemizygosity for Df(2L)al significantly increases post-fertilization embryonic mortality. Paternal hemizygosity has no effect.

(Chandrashekaran, 1995)

Homozygous embryos do not complete head involution and tracheae are disconnected.

(Harbecke and Lengyel, 1995)

Heterozygosity for this deletion has no effect on the mutant ovarian phenotype of ovo^D2.

(Pauli et al., 1995)

Homozygous lethal.

(Korochkina and Golubovsky, 1978)

Heterozygotes have an extreme Minute phenotype with rough eyes.

(Lewis, 1945)

Homozygous lethal heterozygous Minute phenotype

Stocks (4)

Bloomington

3548

Df(2L)al, ds^al/In(2L)Cy, Duox^Cy

Kyoto

107090

Df(2L)al, ds^al/In(2L)Cy, Duox^Cy

101423

Df(2L)al, al^* / In(2L)Cy, E(S)¹ Duox^Cy

101424

Df(2L)al / CyO, Duox^Cy dpy^lv1 pr¹ cn² P{ry^+t7.2=ftz-lacC}

Notes on Origin

Discoverer

E.B. Lewis, 1940.

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DF(21C1-21C7)

(Tyra et al., 2020)

Df(2)al

(Hirose et al., 2001, Golubovsky et al., 1978, Korochkina and Golubovsky, 1978)

Df(2L)a1

(Harris et al., 2003)

Df(2L)al

(Li et al., 2020, Sawyer et al., 2020, Tyra et al., 2020, Han et al., 2014, Song and Lu, 2012, Marygold et al., 2011, Ida et al., 2009, Lee et al., 2008, Crest et al., 2007, Eun et al., 2007, Tyler et al., 2007, Raabe et al., 2004, Zelhof et al., 2003)

Dfal

(Couso and Bishop, 1998)

M(2)21C

(Fauvarque et al., 2001)

Name Synonyms

Deficiency (2L) aristaless

Secondary FlyBase IDs

References (82)

Report Sections

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