[83A6-83A6];[83B6-83B6];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
83A6;83B6
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(3R)ED10257.
The presence of P+PBac{XP5.RB3}BSC549 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3R)BSC549 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d09717 and PBac{RB}Spec2e04478 transposable element insertion sites are 83A6;83B6.