A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}853 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The breakpoints of Df(1)BSC853 predicted from the Release 5 genomic coordinates of the insertion sites of the progenitor transposable elements PBac{RB}e02417 and P{XP}d04369 are X:17126100 ;17455865 and the cytological breakpoints predicted from these coordinates are 15F4;16B4.