A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.WH5}BSC856 was verified using the PCR methods and primers described in FBrf0175003.
The Gene Disruption Project determined the insertion site of the progenitor P{XP}d03141 to be Release 3 genomic coordinate 19811818 on arm 2R. This corresponds to 2R:20655771 and 60E1 on the Release 5 map. The insertion site of the progenitor PBac{WH}f04726 is Release 5 genomic coordinate 2R:20741841 , which corresponds to 60E4. Consequently, the breakpoints of Df(2R)BSC856 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2R:20655771 ;20741841 and the cytological breakpoints predicted from these coordinates are 60E1;60E4.