A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.WH5}BSC866 was verified using the PCR methods and primers described in FBrf0175003.
The presence of the deficiency was confirmed by showing that a 488 base pair fragment spanning the P{SUPor-P}CG2116KG00028 insertion site could not be amplified from Df(1)BSC866/P{SUPor-P}CG2116KG00028 flies using primers 5'-CGATGTATGAAAGCGGAAGGAAGCAAGC-3' and 5'-CCAATTCCCTGACCACAAATTGCG-3'. Also, a 630 base pair fragment spanning the PBac{PB}CG10959c02347 insertion site could not be amplified from Df(1)BSC866/PBac{PB}CG10959c02347 flies using primers 5'-CCGCTCACCAAGACGGTCACGGG-3' and 5'-CCGTGTGCTCCTTGCGACGATGC-3'.
