A set of ~400 overlapping Pacman BAC clones was used to create small duplications (average length 88 kb) covering most of the 22-Mb sequenced portion of the X chromosome. Using the Pacman system (FBrf0194996), the BAC clones were inserted into an attP docking site on chromosome arm 3L; available as homozygous or balanced heterozygous stocks.
Docking-site insertion on 2L used for one line.
A duplication of sequence from the X chromosome (coordinates 1:20016288..20077672 , release 5) derived from the CH321-15K12 BAC clone has been inserted into the PBac{y+-attP-3B}VK00033 docking site on the 3rd chromosome using the P"["acman"]" system. The left end of the duplication is at coordinate 1:20016288 , release 5. The right end of the duplicated sequence is in a repetitive element and so could either end at 1:20077672 or 1:20093231 , release 5.
Homozygous viable and fertile.