A small deletion that eliminates the 5' splicing signal of intron 2 leading to an in-frame premature stop codon.
Steady state level of Adh mRNA is greatly reduced.
Sequence rearrangement prevents the splicing of the first 65bp intron.
No mature mRNA; 6 bp deletion in first intron (106-111); 101-105 substituted by CGATC; splicing defective.
chemical sensitive (with Adhn1)
Flies of the transheterozygous mutant genotypes Adhfn6/Adhn1 and Adhfn6/Adhfn23 both show the same defective phenotypic response to ethanol. These flies experience a hyperactive phase that is of a much lower magnitude than in wild type. The proportion of akinetic flies is substantially higher amongst the Adh mutants than in wild-type flies and the mutants accumulate higher amounts of ethanol. However, the mutants have a normal startle response to ethanol.
Adhfn6 mutants exhibit a severely delayed recovery from ethanol exposure.
Homozygous adult flies can only survive to a maximum of 7% alcohol in the culture medium, whereas heterozygotes can survive at 17% alcohol in the culture medium (wild-type flies can tolerate 20% alcohol in the culture medium). The survival rate of heterozygotes carrying three copies of Adhw.PR on culture medium containing increasing amounts of alcohol is intermediate between that of Adhfn6 homozygotes and wild-type flies.
Sofer.
CRM negative.
Used, carried in P-element vectors along with other Adh alleles, to study gene conversion and unequal crossing over to re-generate "Adh+".