FlyBase Allele Report: Dmel\ey[2]

General Information

Symbol

Dmel\ey²

Species

D. melanogaster

Name

FlyBase ID

FBal0003921

Feature type

allele

Associated gene

Dmel\ey

Associated Insertion(s)

Doc{}ey²

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Niwa et al., 2004)

hypomorphic allele - genetic evidence

(Noveen et al., 2000)

Mutagen

spontaneous

(Quiring et al., 1994)

Progenitor genotype

Associated Insertion(s)

Doc{}ey²

Cytology

Description

Maps to intron preceding exon 3. The Doc insertion in ey² and the flea insertion in ey^R map within 75 bases of each other.

(Hauck et al., 1999)

Doc element insertion within intron 1.

(Xu et al., 1999)

Doc insertion into an eye-specific enhancer element.

(Callaerts et al., 1995)

Insertion of a Doc element near the 5' end of the ey gene into a regulatory element required for expression in the eye primordia.

(Quiring et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

4:710,145..710,145 [+]

(Pauli et al., 2005, Jang et al., 2003, Hauck et al., 1999, Xu et al., 1999, Callaerts et al., 1995, Quiring et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Niwa et al., 2004)

partially lethal | recessive

(Callaerts et al., 2001)

viable (with ey^D1Da)

(Callaerts et al., 2001)

viable (with ey^JD)

(Callaerts et al., 2001)

visible

(Callaerts et al., 2001)

visible (with ey^OK107-16)

(Clements et al., 2009)

visible | recessive

(Pignoni, 2001.1.26, Martini et al., 2000, Halder et al., 1998)

Phenotype Manifest In

adult optic lobe

(Fischbach, 1983)

antenna | ectopic, with Scer\GAL4^ey.PH, ey^ΔHD.UAS

(Punzo et al., 2004)

eye

(Callaerts et al., 2001, Pignoni, 2001.1.26, Kurata et al., 2000, Martini et al., 2000, Halder et al., 1998, Fischbach, 1983, Bulthoff, 1982)

eye, with Scer\GAL4^dpp.blk1, ey^ΔPD.UAS

(Punzo et al., 2001)

eye, with Scer\GAL4^ey.PH, ey^ΔHD.UAS

(Punzo et al., 2004)

eye (with ey^D)

(Jang et al., 2003, Kronhamn et al., 2002)

eye (with ey^OK107-16)

(Clements et al., 2009)

eye | ectopic, with Scer\GAL4^dpp.blk1, ey^ΔHD.UAS

(Punzo et al., 2001)

eye-antennal disc

(Halder et al., 1998)

eye disc

(Anderson et al., 2006, Niwa et al., 2004)

mushroom body medial lobe

(Noveen et al., 2000)

ommatidium

(Halder et al., 1998)

retina

(Anderson et al., 2006)

Detailed Description

Statement

Reference

ey² heterozygotes do not exhibit any size alterations or roughness in eyes when compared to control adults.

(Hoang et al., 2010)

ey² mutants have a small eye disc and show a complete loss of the retina.

(Anderson et al., 2006)

There is severe apoptosis in the early third instar larval eye disc in ey² mutants.

(Niwa et al., 2004)

Small, antenna-like outgrowths are seen in place of eyes in a small percentage of ey²/ey² ey^{Scer\UAS.ΔHD} Scer\GAL4^ey.PH flies.

(Punzo et al., 2004)

Mutant adults exhibit a variable eye phenotype.

(Jang et al., 2003)

ey²/ey^D animals have small eyes.

(Kronhamn et al., 2002)

Homozygotes show 45% lethality. Eyes are reduced in size in homozygotes; 71% have eyes that are 0-25% of wild-type size, 23% have eyes that are 25-50% of wild-type size, 6% have eyes that are 50-75% of wild-type size and 0% have eyes that are 75-100% of wild-type size. Homozygotes have no overt defects in the mushroom body or central complex. 100% of ey²/ey^D1Da and ey²/ey^JD flies have eyes which are 75-100% of wild-type size and they have no overt defects in the mushroom body or central complex.

(Callaerts et al., 2001)

Eye is reduced in size.

(Pignoni, 2001.1.26)

Expression of ey^{Scer\UAS.ΔHD} when driven by Scer\GAL4^dpp.blk1 in a ey² background leads to ectopic eyes.

(Punzo et al., 2001)

Homozygous adults have no apparent mushroom body phenotype. The eyes are variably reduced.

(Martini et al., 2000)

Medial lobes of the mushroom body are fused in about 35% of second instar larvae. Embryonic pattern of mushroom body neuroblasts is apparently normal.

(Noveen et al., 2000)

Mutant phenotype is variable: 3% of flies show a wild type eye. In combination with ey^mEYE-ey, 55% show almost wild type-sized eyes. The anlagen of the eye are formed normally in mutant embryos, but by the late third instar larva the eye portion (though not the antennal portion) of the eye-antennal disc is severely reduced. Ommatidial cluster development fails. Eye discs show massive cell death, similar to the phenotype for so¹ and eya¹.

(Halder et al., 1998)

Displays locomotor activity rhythm with an approximately 24h period.

(Vosshall and Young, 1995)

Flies have a variable number of facets on one or both sides. The reduction in the medulla of the optic lobe in the adult is most severe in the distal region.

(Fischbach, 1983)

Rudimentary compound eyes.

(Bulthoff, 1982)

eye size relative to that of wild type: .25-.50 Eye reduced to one-half to one-fourth wild-type area with relatively little variability. Cephalic complex smaller than wild type 25 hr after hatching from egg at 27^oC; subsequent growth rate same as wild type (Medvedev, 1935a; 1935b; Steinberg, 1944). RK1.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ey² has visible | recessive phenotype, enhanceable by so[+]/so³

(Pignoni, 2001.1.26)

ey² has visible phenotype, enhanceable by dac^P/dac^P

(Martini et al., 2000)

Enhancer of

Statement

Reference

ey² is an enhancer of lethal phenotype of Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

ey²/ey² is an enhancer of visible phenotype of dac^P

(Martini et al., 2000)

Suppressor of

Statement

Reference

ey[+]/ey² is a suppressor | partially of abnormal size | adult stage phenotype of CtBP^GSA396, Scer\GAL4^ey.PU

(Hoang et al., 2010)

ey[+]/ey² is a suppressor | partially of visible | adult stage phenotype of CtBP^GSA396, Scer\GAL4^ey.PU

(Hoang et al., 2010)

NOT Suppressor of

Statement

Reference

ey² is a non-suppressor of visible phenotype of Optix^UAS.cSa, Scer\GAL4^dpp.blk1

(Seimiya and Gehring, 2000)

Other

Statement

Reference

Optix^UAS.cSa, Scer\GAL4^dpp.blk1, ey² has visible phenotype

(Seimiya and Gehring, 2000)

Opt^G, ey² has visible | heat sensitive phenotype

(Postlethwait, 1974)

Phenotype Manifest In

Enhanced by

Statement

Reference

ey² has eye phenotype, enhanceable by so[+]/so³

(Pignoni, 2001.1.26)

ey² has eye phenotype, enhanceable by dac^P/dac^P

(Martini et al., 2000)

ey² has eye | heat sensitive phenotype, enhanceable by Opt^G

(Postlethwait, 1974)

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ey.PH, ey² has antenna | ectopic phenotype, suppressible | partially by ey^ΔHD.UAS

(Punzo et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^ey.PH, ey² has antenna | ectopic phenotype, suppressible by ey^ΔPD.UAS

(Punzo et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^ey.PH, ey² has eye phenotype, suppressible | partially by ey^ΔHD.UAS

(Punzo et al., 2004)

ey^D/ey² has eye phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/eyg^UAS.lune

(Jang et al., 2003)

NOT suppressed by

Statement

Reference

ey² has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

ey² is an enhancer of eye phenotype of pb^Rev3.HSPB

(Benassayag et al., 2003)

ey²/ey² is an enhancer of eye phenotype of dac^P

(Martini et al., 2000)

ey² is an enhancer of ocellus | ectopic phenotype of Optix^UAS.cSa, Scer\GAL4^dpp.blk1

(Seimiya and Gehring, 2000)

NOT Enhancer of

Statement

Reference

ey²/ey² is a non-enhancer of phenotype of dac^P

(Martini et al., 2000)

ey²/ey² is a non-enhancer of adult mushroom body gamma-lobe phenotype of dac^P

(Martini et al., 2000)

ey²/ey² is a non-enhancer of mushroom body phenotype of dac^P

(Martini et al., 2000)

Suppressor of

Statement

Reference

ey[+]/ey² is a suppressor | partially of eye phenotype of CtBP^GSA396, Scer\GAL4^ey.PU

(Hoang et al., 2010)

ey² is a suppressor of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, toy^UAS.cCa

(Czerny et al., 1999)

NOT Suppressor of

Statement

Reference

Scer\GAL4^dpp.blk1, ey²/ey² is a non-suppressor of eye | ectopic phenotype of toy^ΔHD.UAS

(Punzo et al., 2004)

Scer\GAL4^dpp.blk1, ey²/ey² is a non-suppressor of eye | ectopic phenotype of Mmus\Pax6^UAS.cHa

(Punzo et al., 2004)

Scer\GAL4^dpp.blk1, ey²/ey² is a non-suppressor of eye | ectopic phenotype of toy^UAS.cCa

(Punzo et al., 2004)

ey² is a non-suppressor of eye | ectopic phenotype of Scer\GAL4^upd1-E132, eyg^UAS.lune

(Jang et al., 2003)

ey² is a non-suppressor of eye | ectopic phenotype of Optix^UAS.cSa, Scer\GAL4^dpp.blk1

(Seimiya and Gehring, 2000)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PI, ey² has eye-antennal disc phenotype

(Niwa et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^ey.PH, ey² has antennal disc | ectopic phenotype

(Punzo et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^ey.PH, ey² has eye-antennal disc phenotype

(Punzo et al., 2004)

N^ICN.UAS, Scer\GAL4^ey.PH, ey² has eye | ectopic phenotype

(Kurata et al., 2000)

N^ICN.UAS, Scer\GAL4^ey.PH, ey² has antenna | ectopic phenotype

(Kurata et al., 2000)

Opt^G, ey² has wing | ectopic | heat sensitive phenotype

(Postlethwait, 1974)

Additional Comments

Genetic Interactions

Statement

Reference

The ectopic eyes seen in flies in which Scer\GAL4^dpp.blk1 drives expression from toy^Scer\UAS.cCa, toy^{ΔHD.Scer\UAS} or Mmus\Pax6^Scer\UAS.cHa are not suppressed by ey²/ey².

(Punzo et al., 2004)

Eyes of ey²/ey²; so³/+ flies are more severely reduced than those of ey²/ey²; +/+ flies.

(Pignoni, 2001.1.26)

Expression of N^ICN.Scer\UAS driven by Scer\GAL4^ey.PH in a ey² background, produced flies with reduced eyes. About 15% of these flies had both a reduced original and a reduced ectopic eye. In addition, ectopic antennae were seen in about 1/4 of these flies.

(Kurata et al., 2000)

The eyes of dac^P; ey² double homozygotes are consistently very small or absent.

(Martini et al., 2000)

Ectopic eyes are still produced if Optix^Scer\UAS.cSa is expressed under the control of Scer\GAL4^dpp.blk1 in an ey² background. The efficiency of occurrence of ectopic eyes is not changed from when Optix^Scer\UAS.cSa is expressed under the control of Scer\GAL4^dpp.blk1 in a wild-type background, but the frequency of induction of extra ocelli is increased.

(Seimiya and Gehring, 2000)

ey² blocks the formation of ectopic eyes caused by toy^Scer\UAS.cCa, Scer\GAL4^dpp.blk1.

(Czerny et al., 1999)

Ectopic eyes are not produced when eya^Scer\UAS.cPa and so^Scer\UAS.cPa are coexpressed under the control of Scer\GAL4^dpp.blk1 in an ey² background, although growth alterations and patches of red pigment cells on the legs are still seen.

(Pignoni et al., 1997)

In the presence of Opt mutations, ey² flies exhibit protrusions of wing tissue from the region of the eye; development is normal at 17^oC, but homeotic transformation is observed at 29^oC; the temperature-sensitive period is during the second larval instar. Protrusions from the anterior portion of the eye have the triple row of bristles characteristic of the anterior wing margin; the fronto-orbital region of the head produces bristles with bracts characteristic of the costa in the proximal wing.

(Postlethwait, 1974)

Xenogenetic Interactions

Statement

Reference

In ey² mutant eye-antennal discs that contain clones of cells expressing BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PI, either no endogenous eyes form, or a partial eye forms (as scored by expression of gl) in the ventral region.

(Niwa et al., 2004)

In ey²/ey² BacA\p35^Scer\UAS.cHa; Scer\GAL4^ey.PH third instar larvae, the eye portion of the eye-antennal disc is replaced by a duplication of the antennal half of the disc. The resulting adults have an extra antenna in place of each eye. This ectopic antenna phenotype is suppressed by ey^{Scer\UAS.ΔPD}, which does not rescue eye development. In contrast ey^{Scer\UAS.ΔHD} can both rescue eye development and suppress ectopic antenna formation, although this suppression is incompletely penetrant for both aspects of the phenotype.

(Punzo et al., 2004)

Complementation and Rescue Data

Fails to complement

ey¹¹

(Benassayag et al., 2003)

ey^EH

(Benassayag et al., 2003)

Rescued by

ey² is rescued by ey^ΔHD.UAS/Scer\GAL4^ey.PH

(Punzo et al., 2001)

Partially rescued by

ey² is partially rescued by ey^mEYE-ey

(Halder et al., 1998)

Not rescued by

ey² is not rescued by ey^ΔPD.UAS/Scer\GAL4^ey.PH

(Punzo et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

648

ey²

6352

y¹; Opt^G; Dp(1;3)sc^J4, y⁺/l(3)*^*; ey²

Kyoto

106026

ey²

108749

y¹; Opt^G; Dp(1;3)sc^J4/+; ey²

102058

y¹ ; Opt^G; Dp(1;3)sc^J4 / + ; ey²

Notes on Origin

Discoverer

Nonidez, 1919.

Comments

No mutation has been detected by sequencing in the exons of the toy gene in ey² mutants.

(Czerny et al., 1999)

Normal orientation effects in the fixation Y-maze.

(Bulthoff, 1982)

Loss of ey expression.

(Callaerts et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ey²

(Baker et al., 2018, Lopes and Casares, 2015, Hoang et al., 2010, Clements et al., 2009, Yoshioka et al., 2007, Anderson et al., 2006, Punzo et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (43)

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