FlyBase Allele Report: Dmel\fu[41]

General Information

Symbol

Dmel\fu⁴¹

Species

D. melanogaster

Name

FlyBase ID

FBal0004874

Feature type

allele

Associated gene

Dmel\fu

Associated Insertion(s)

Carried in Construct

Also Known As

fu^A

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Pham et al., 1995, Therond et al., 1993)

Mutagen

X ray

(Preat et al., 1993, Busson et al., 1988, Mariol et al., 1987)

Progenitor genotype

Cytology

Description

Class II fu allele, affects the carboxy domain of the protein by changing the open reading frame in the 5' region.

(Preat et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Many fu⁴¹ mutant germaria contain increased numbers of spectrosome-containing GSC-like cells. Approximately 10% of germaria contain the normal number of GSC-like cells (<5), nearly 60% contain 5-10 cells and 30% of germaria are tumorous, containing in excess of 10 cells.

(Xia et al., 2010)

fu⁴¹ mutants exhibit a 'fused' wing phenotype, with fusion observed between L3 and L4.

(Liu et al., 2007)

In 4 day old fu⁴¹ homozygous females, only 7% of ovarioles are tumorous, but this has increased to 19% by 10 days. Tumorous follicles in these ovarioles consist of tens to hundreds of germ-line cells with small non-polyploid nuclei enveloped by a regular follicular epithelium. No increase in the rate of cell division of germline stem cells in the stem cell niche of the germarium is seen in these animals.

(Narbonne-Reveau et al., 2006)

Mutants show fusion of wing veins L3 and L4 both proximally and distally, with intervein tissue remaining between the two veins in the medial part of the wing.

(Glise et al., 2002)

Mutant embryos die before hatching with mirror-image duplication of the abdominal denticle belts. Dorsal cuticle is mostly covered by a lawn of type 4 fine hairs. Cells type 1 are missing. The extent of naked cuticle (type 2 cells) is greatly reduced. Cells type 3 are usually present but sometimes missing. Type 4 cells are unaffected.

(Therond et al., 1999)

Does not suppress the phenotype produced by ectopic expression of hh^Scer\UAS.cCa using Scer\GAL4^30A.

(Sanchez-Herrero et al., 1996)

When in combination with Su(fu)⁺ individuals exhibit a partially suppressed fu wing phenotype. Loss of one copy of Su(fu)⁺ causes a cos phenotype. fu⁴¹/fu^K33R individuals display a strong fu wing phenotype, loss of proximal wing vein L4.

(Therond et al., 1996)

Heterozygotes carrying one copy of Su(fu)⁺ are pharate adult lethal and display a cos wing phenotype. The presence of P{SUFU100} or P{SUFU200} in these individuals produces viable males with a fu⁴¹ wing phenotype, the transposons fully compensate the decreased amount of Su(fu)⁺ product. With P{SUFU000} no males are recovered, consistent with lack of Su(fu)⁺ function. Heterozygotes carrying two copies are viable and display a fu wing phenotype. When the number of Su(fu)⁺ doses (via P{SUFU100}) in males is greater than two viability decreases, the decrease in viability is correlated with an enhancement of the fu wing phenotype. Decrease in viability is more pronounced when the transgene is provided by the mother. Viability of fu⁴¹ males is also affected when the Su(fu)⁺ activity decreases, the decrease in viability is associated with an anterior wing duplication phenotype (similar to cos mutant phenotype).

(Pham et al., 1995)

fu⁴¹ mutant embryos have pattern deletions in the posterior part of each segment in the cuticle, which are replaced by mirror duplications of the anterior part. The phenotype of fu⁴¹ ftz¹³, fu⁴¹ en^LI034, and fu⁴¹ Ubx^9.22 double mutants can be interpreted as an addition of the corresponding single mutant phenotypes. The phenotype of fu⁴¹ h²⁵ double mutants can be interpreted as an addition of the corresponding single mutant phenotypes, except that the naked cuticle of segments A5 and A7 which is absent in the h²⁵ single mutant, is replaced by a mirror duplication of the remaining denticle belts in the fu h double mutant. The ventral cuticle pattern is nearly wild type in fu⁴¹ nkd² double mutants.

(Limbourg-Bouchon et al., 1991)

Anomalies of adult cuticular structures and tumorous ovaries.

(Preat et al., 1990)

strong wing phenotype - veins L3 and L4 fused all along length; intermediate viability phenotype - ratio of the number of fused flies to wild type in fu/+ X fu/Y is 0.5-0.75; weak fecundity phenotype - ratio of eggs laid by fu/fu versus fu/+ sisters is 0.5-1.0; strong maternal effect on segmentation - percent of embryos with all segments entirely duplicated is 70-100%.

(Busson et al., 1988)

Strong embryonic phenotype: the posterior portion of each segment is deleted and replaced with a mirror-image duplication of the anterior portion. Embryos die just before eclosion.

(Mariol et al., 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fu⁴¹ has hyperplasia | adult stage phenotype, enhanceable by tkv^ca.bam.GFP

(Xia et al., 2010)

Suppressed by

Statement

Reference

fu⁴¹ has hyperplasia | adult stage phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Dad^UASp.cJa

(Xia et al., 2010)

Enhancer of

Statement

Reference

fu⁴¹ is an enhancer of lethal | pupal stage phenotype of Scer\GAL4^da.G32, Su(fu)^UAS.cDa

(Dussillol-Godar et al., 2006)

Suppressor of

Statement

Reference

fu⁴¹ is a suppressor of visible | dominant phenotype of hh^Mrt

(Alves et al., 1998)

Other

Statement

Reference

ci^Ce-4, fu⁴¹ has visible | dominant phenotype

(Alves et al., 1998)

ci^Ce-4, fu⁴¹ has visible | recessive phenotype

(Alves et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

fu⁴¹ has ovary phenotype, enhanceable by tkv^ca.bam.GFP

(Xia et al., 2010)

fu⁴¹ has germarium phenotype, enhanceable by tkv^ca.bam.GFP

(Xia et al., 2010)

fu⁴¹ has female germline cell | increased number phenotype, enhanceable by tkv^ca.bam.GFP

(Xia et al., 2010)

fu⁴¹ has phenotype, enhanceable by Su(fu)^LP

(Preat et al., 1993)

Suppressed by

Statement

Reference

fu⁴¹ has ovary phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Dad^UASp.cJa

(Xia et al., 2010)

fu⁴¹ has germarium phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Dad^UASp.cJa

(Xia et al., 2010)

fu⁴¹ has female germline cell | increased number phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Dad^UASp.cJa

(Xia et al., 2010)

Enhancer of

Statement

Reference

fu⁴¹ is an enhancer of wing phenotype of Scer\GAL4^da.G32, Su(fu)^UAS.cDa

(Dussillol-Godar et al., 2006)

fu⁴¹ is an enhancer of sex comb | increased number phenotype of Scer\GAL4^da.G32, Su(fu)^UAS.cDa

(Dussillol-Godar et al., 2006)

fu⁴¹ is an enhancer of metathoracic leg phenotype of Scer\GAL4^da.G32, Su(fu)^UAS.cDa

(Dussillol-Godar et al., 2006)

fu⁴¹ is an enhancer of prothoracic leg phenotype of Su(fu)^UAS.cDa

(Dussillol-Godar et al., 2006)

Suppressor of

Statement

Reference

fu⁴¹ is a suppressor of wing vein L2 phenotype of hh^Mrt

(Alves et al., 1998)

fu⁴¹ is a suppressor of wing | anterior | distal phenotype of hh^Mrt

(Alves et al., 1998)

Other

Statement

Reference

Scer\GAL4^da.G32, Su(fu)^UAS.cDa, fu⁴¹ has wing vein L2 phenotype

(Dussillol-Godar et al., 2006)

Scer\GAL4^da.G32, Su(fu)^UAS.cDa, fu⁴¹ has wing vein L3 phenotype

(Dussillol-Godar et al., 2006)

Scer\GAL4^da.G32, Su(fu)^UAS.cDa, fu⁴¹ has wing margin phenotype

(Dussillol-Godar et al., 2006)

Scer\GAL4^da.G32, Su(fu)^UAS.cDa, fu⁴¹ has wing vein L4 phenotype

(Dussillol-Godar et al., 2006)

Su(fu)^LP, fu⁴¹ has wing disc | anterior phenotype

(Alves et al., 1998)

ci^Ce-4, fu⁴¹ has scutellum phenotype

(Alves et al., 1998)

ci^Ce-4, fu⁴¹ has wing phenotype

(Alves et al., 1998)

ci^Ce-4, fu⁴¹ has adult thorax phenotype

(Alves et al., 1998)

Su(fu)^LP/Su(fu)[+], fu⁴¹ has wing disc | anterior phenotype

(Alves et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dad^{Scer\UAS.P\T.cJa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the increase in spectrosome-containing GSC-like cells seen in fu⁴¹ mutant germaria.

Expression of tkv^{ca.bam.T:Avic\GFP} enhances the increased number of spectrosome-containing GSC-like cells seen in fu⁴¹ mutant germaria.

Homozygous lack^15C has no effect on the increased number of spectrosome-containing GSC-like cells seen in fu⁴¹ mutant germaria.

(Xia et al., 2010)

In escaper flies that express Su(fu)^Scer\UAS.cDa, under the control of Scer\GAL4^da.G32, in a fu⁴¹ background, the wing phenotype is enhanced compared to flies expressing the transgene in a wild-type background. fu⁴¹, Su(fu)^Scer\UAS.cDa, Scer\GAL4^da.G32 wings show anterior duplications but also have a truncated vein 2, an enlargement of the domain between vein 2 and the margin, and veins 3 and 4 are completely fused with a large delta at the margin. These flies also show an enhancement of the leg phenotype seen in Su(fu)^Scer\UAS.cDa, Scer\GAL4^da.G32 flies.

(Dussillol-Godar et al., 2006)

fu⁴¹; Su(fu)^LP/+ wing imaginal discs show overgrowth of the anterior compartment. fu⁴¹; ci^Ce-4/+ flies generally die as pupae, although some adults eclose. The latter have small wings, a reduced thorax and lack the scutellum. Partially suppresses the wing phenotype of hh^Mrt heterozygotes.

(Alves et al., 1998)

When in combination with Su(fu)^LP, Su(fu)^12d, cos³, Df(3R)kar3Q or Df(3R)kar-Sz11 heterozygotes individuals die as pupae. ptc mutations do not suppress the fu phenotype, fu¹; ptc⁹/ptc⁹ embryos show no naked cuticle.

(Preat et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fu⁴¹ is rescued by fu^+t5.1

(Xia et al., 2010)

fu⁴¹ is rescued by Scer\GAL4^arm.PS/fu^UAS.Tag:HA

(Liu et al., 2007)

fu⁴¹ is rescued by fu^hs.PT

(Therond et al., 1999)

fu⁴¹ is rescued by fu^UAS.cTa/Scer\GAL4^wg.PM

(Therond et al., 1999)

fu⁴¹ is rescued by Scer\GAL4^ptc-559.1/fu^UAS.cAa

(Alves et al., 1998)

fu⁴¹ is rescued by fu^+t5.1

(Therond et al., 1993)

fu⁴¹ is rescued by fu^+t7.4

(Therond et al., 1993)

fu⁴¹ is rescued by fu^+t5.1

(Preat et al., 1990)

Partially rescued by

fu⁴¹ is partially rescued by fu^UAS.cAa/Scer\GAL4^dpp.blk1

(Alves et al., 1998)

Not rescued by

fu⁴¹ is not rescued by fu^KD

(Xia et al., 2010)

fu⁴¹ is not rescued by fu^{G13V.UAS.Tag:HA}/Scer\GAL4^arm.PS

(Liu et al., 2007)

fu⁴¹ is not rescued by Scer\GAL4^en-e16E/fu^UAS.cTa

(Therond et al., 1999)

Comments

Expression of fu^+t5.1 rescues the increase in GSC-like cells seen in fu⁴¹ mutant germaria.

Expression of fu^KD fails to rescue the increase in GSC-like cells seen in fu⁴¹ mutant germaria.

(Xia et al., 2010)

Wing and embryonic phenotypes rescued by fu^+t5.1 and fu^+t7.4. Complete maternal rescue, 90% of embryos hatch to larvae and of these larvae 70% reach adulthood and those carrying fu^+t5.1 or fu^+t7.4 have wild type wings.

(Therond et al., 1993)

Mutant phenotype has been rescued by the introduction of the wild type fu gene by P element mediated transformation.

(Preat et al., 1990)

Mutant phenotype is partially rescued by injected fu^CosC4 and less so by injected fu^cMa.

(Mariol et al., 1987)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7203

w¹ fu⁴¹/FM6

Notes on Origin

Discoverer

Comments

Class II fu allele.

(Pham et al., 1995)

Class II mutation based on interaction with Su(fu)^LP, suppression of embryonic and adult phenotype but display an additional phenotype.

(Therond et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

FuA

(Sardi et al., 2021)

fu⁴¹

fu^A

(Ranieri et al., 2014, Xia et al., 2010, Liu et al., 2007, Dussillol-Godar et al., 2006, Narbonne-Reveau et al., 2006, Vegh and Basler, 2003, Besse et al., 2002, Glise et al., 2002, Methot and Basler, 2000, Therond et al., 1999, Alves et al., 1998, Sanchez-Herrero et al., 1996, Therond et al., 1996, Pham et al., 1995, Preat et al., 1993, Therond et al., 1993, Preat et al., 1990, Busson et al., 1988, Mariol et al., 1987)

fu^a

(Limbourg-Bouchon et al., 1991)

Name Synonyms

Secondary FlyBase IDs

References (21)

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