11bp deletion of bases 379 to 389. This deletion results in a frameshift and produces a truncated protein that diverges from wild-type grn after 52 amino acids and lacks both zinc fingers.
grn7L mutant embryos do not show morphological abnormalities in the midgut.
In grn7L/Df(3R)dsx3 mutants the intersegmental nerve (ISN) motor axons are stalled at muscles 2/10, leading to a near complete loss of innervation (~12-18%) of the dorsal-most muscles 1/9. In addition, grn7L/Df(3R)dsx3 mutants display aberrant innervation of muscle 8. In affected hemisegments, a grossly normal pattern of axonal projections to the dorsal muscles 2/10 is observed, indicating that aCC and/or RP2, and at least one of the U motoneurons project aberrantly to muscle 8.
grn7L/Df(3R)dsx3 mutants display a partially penetrant muscle patterning phenotype, evident as an imprecise insertion of muscles 21-24 into the body wall.
In grn7L/Df(3R)dsx3 mutants, the intersegmental nerve fails to innervate muscles 1/9, but axonal projections are still observed contacting muscles 2/10.
In grn7L/Df(3R)dsx3 mutants, approximately 36% of hemisegments show ectopic innervation of muscle 8, together with defasciculation of aCC and RP2 motor axons. Muscle 9 is not innervated (by U1), while U axon terminals contact muscles field 2/10.
grn7L/Df(3R)dsx10M embryos have an abnormal head skeleton with aberrant lateralgraten and dorsal bridge. The stigmatophore does not protrude (in contrast to wild type) and the filzkorper is short. Other cuticular structures are normal. The number of stigmatophore cells abutting the posterior spiracular chamber and the number of cell tiers does not change during development in mutant embryos, in contrast to wild type. Germline clonal analysis indicates that grn is not required maternally. No abnormalities are seen when homozygous clones occupy the wing or distal leg segments. Clones on the proximal leg segments often severely affect the shape of the femur and tibia, which become shorter and broader than in wild type. In these cases the segments are approximately 1/3 shorter and 1/3 broader than normal, but leg segmentation is normal. The number of leg trichomes is normal. The shape of homozygous clones in the leg disc is similar to that of wild-type twin clones.
No changes in phenotype of tor13D embryos.
grn7L has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by unc-5UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4eve.RN2
grn7L/Df(3R)dsx3 has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4eve.RN2/zfh1UAS.cPa
grn7L, unc-5[+]/unc-58 has abnormal neuroanatomy | embryonic stage phenotype
grn7L has larval intersegmental nerve phenotype, suppressible | partially by unc-5UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4eve.RN2
grn7L/Df(3R)dsx3 has larval DA1 motor neuron phenotype, suppressible | partially by Scer\GAL4eve.RN2/zfh1UAS.cPa
grn7L/Df(3R)dsx3 has larval RP2 motor neuron phenotype, suppressible | partially by Scer\GAL4eve.RN2/zfh1UAS.cPa
grn7L, unc-5[+]/unc-58 has larval intersegmental nerve phenotype
unc-58/+ ; grn7L/+ double heterozygous embryos show guidance defects in the intersegmental nerve.
The defects in intersegmental nerve innervation of muscles 1/9 are partially rescued by expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eve.RN2.
Expression of zfh1Scer\UAS.cPa in the aCC/RP2 neurons, under the control of Scer\GAL4eve.RN2 can only partially rescue the grn7L/Df(3R)dsx3 motoneuron phenotype, with muscle 1/9 innervation increasing to 34% compared with the more severe (approximately 12%) grn7L/Df(3R)dsx3 mutant phenotype.
grn7L/Df(3R)dsx3 is rescued by Scer\GAL4eve.RN2/grnUAS.cBa
grn7L/Df(3R)dsx3 is partially rescued by grnUAS.cBa/Scer\GAL4eve.CQ2
Expression of grnScer\UAS.cBa in the aCC/RP2 neurons, under the control of Scer\GAL4eve.RN2 efficiently rescues grn7L/Df(3R)dsx3 mutant axon pathfinding (with 100% muscle 1/9 innervation). By contrast, expression of grnScer\UAS.cBa in the U motoneurons, under the control of Scer\GAL4eve.CQ2 only partially rescues the grn7L/Df(3R)dsx3 mutant phenotypes, with approximately 54% of muscles 1/9 being innervated.
