FlyBase Allele Report: Dmel\l(2)gl[334]

General Information

Symbol

Dmel\l(2)gl³³⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0009238

Feature type

allele

Associated gene

Dmel\l(2)gl

Associated Insertion(s)

Carried in Construct

Also Known As

lgl³³⁴, 334, l(2)gl^U334

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

spontaneous

natural population

(Mechler et al., 1985)

Progenitor genotype

Cytology

Description

Distal portion of l(2)gl and telomeric flanking sequences are deleted.

(Opper et al., 1987)

Deletion of sequences from 0 to 10kb (coordinates with respect to proximal end of a cloned l(2)gl region, close to the tip of 2L).

(Mechler et al., 1985)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with l(2)gl⁴)

(Bell et al., 2015)

abnormal neuroanatomy

(Lee et al., 2006)

abnormal neuroanatomy | larval stage (with l(2)gl³⁶⁴⁴)

(Haenfler et al., 2012)

increased cell number | larval stage

(Lee et al., 2006)

increased cell number | larval stage | somatic clone

(Lee et al., 2006)

lethal

Phenotype Manifest In

dorsal mesothoracic disc & epithelial cell

(Rolls et al., 2003)

embryonic/larval brain

embryonic/larval brain | third instar larval stage

(Rolls et al., 2003)

embryonic/larval brain | third instar larval stage (with l(2)gl⁴)

(Rolls et al., 2003)

embryonic/larval salivary gland (with l(2)gl⁴)

(Farkas and Mechler, 2000)

imaginal disc

(Rolls et al., 2003)

imaginal disc epithelial cell (with l(2)gl⁴), with Scer\GAL4^hh.PU, l(2)gl^ASA.UAS.GFP

(Bell et al., 2015)

imaginal disc epithelial cell (with l(2)gl⁴), with Scer\GAL4^hh.PU, l(2)gl^{UAS.Tag:Myr(Unk)}

(Bell et al., 2015)

intermediate neuronal progenitor | larval stage | increased number (with l(2)gl³⁶⁴⁴)

(Haenfler et al., 2012)

larval brain & neuroblast | supernumerary

(Lee et al., 2006)

larval neuroblast | third instar larval stage

(Rolls et al., 2003)

leg disc

(Rolls et al., 2003)

mitotic spindle (with l(2)gl⁴), with Scer\GAL4^hh.PU, l(2)gl^ASA.UAS.GFP

(Bell et al., 2015)

mitotic spindle (with l(2)gl⁴), with Scer\GAL4^hh.PU, l(2)gl^{UAS.Tag:Myr(Unk)}

(Bell et al., 2015)

type II neuroblast | larval stage | increased number (with l(2)gl³⁶⁴⁴)

(Haenfler et al., 2012)

type I neuroblast | larval stage | increased number (with l(2)gl³⁶⁴⁴)

(Haenfler et al., 2012)

ventral thoracic disc & epithelial cell

(Rolls et al., 2003)

wing disc

(Rolls et al., 2003)

wing disc (with l(2)gl⁴), with Scer\GAL4^hh.PU, l(2)gl^ASA.UAS.GFP

(Bell et al., 2015)

wing disc (with l(2)gl⁴), with Scer\GAL4^hh.PU, l(2)gl^{UAS.Tag:Myr(Unk)}

(Bell et al., 2015)

Detailed Description

Statement

Reference

Expression of Scer\GAL4^hh.PU>l(2)gl^{ASA.Scer\UAS.T:Avic\GFP} rescues the cell polarity defects in l(2)gl⁴/l(2)gl³³⁴ mutant wing disc epithelia, but reveals spindle orientation defects.

Expression of Scer\GAL4^hh.PU>l(2)gl^{Scer\UAS.T:Uuuu\Myr-Unk} rescues the cell polarity defects in l(2)gl⁴/l(2)gl³³⁴ mutant wing disc epithelia, but reveals spindle orientation defects.

(Bell et al., 2015)

At 24 hours after clone induction, type I and II neuroblast clones in l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains contain a single neuroblast, as occurs in wild type. However, at 48 hours after clone induction, the mutant type I and II neuroblast clones contain supernumerary neuroblasts.

Intermediate neural progenitor cell derived clones in l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains contain multiple supernumerary type II neuroblasts.

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains contain an increased number of type II neuroblasts and intermediate neural progenitor cells compared to wild type.

(Haenfler et al., 2012)

Mutants show an increased number of brain neuroblasts at 96 hours after larval hatching compared to controls. Mutant neuroblast clones in the larval brain often contain two neuroblasts, and can contain up to six neuroblasts, in contrast to control clones which contain a single neuroblast.

(Lee et al., 2006)

l(2)gl⁴/l(2)gl³³⁴ L3 larval brains are larger in volume (65% larger) than l(2)gl³³⁴/+ brains. l(2)gl³³⁴/l(2)gl³³⁴ L3 larval brains possess 136 +/- 12 neuroblasts compared to a l(2)gl³³⁴/+ value of 92 +/- 6. Homozygous l(2)gl³³⁴ L3 larval imaginal discs are enlarged compared to l(2)gl³³⁴ heterozygous discs. They don't exhibit a monolayer at L2/L3 larval stages and have a ~49% increase in the number of neuroblasts compared to controls. l(2)gl³³⁴ mutant third instar leg and wing imaginal discs are highly enlarged and disorganised, and often fuse with each other or nearby tissues. At the cellular level, mutant epithelial cells are round instead of columnar, with an expanded apical membrane domain.

(Rolls et al., 2003)

Salivary glands isolated from 8 day old third instar l(2)gl³³⁴/l(2)gl⁴ larvae and maintained in Schneider culture medium for 24 hours are considerably reduced in size compared to wild-type controls and have a "bunch of grapes" shape. The cells of the mutant glands are nearly spherical in form with a reduced surface of cell-to-cell contact. Administration of 10[-5]M 20-hydroxyecdysone fails to induce the histolysis of the mutant glands. No sign of vacuolisation or abnormal pattern of organelle distribution can be detected in treated mutant salivary gland cells and the overall organisation of the glands and structure of the cells is similar to those of untreated mutant glands.

(Farkas and Mechler, 2000)

l(2)gl⁵⁵⁸/l(2)gl³³⁴ transheterozygotes show temperature-sensitive viability. The temperature-sensitive period is biphasic; the first phase is embryonic and the second phase is in the third larval-prepupal stage.

(Sokolova and Golubovsky, 1979)

Some Df(2L)U558/l(2)gl³³⁴ flies are viable. The viability is affected by temperature; the temperature sensitive period is diphasic at 17^oC and 29^oC.

(Sokolova and Golubovsky, 1978)

18.4% of mutants die as larvae, 81.6% as pupae. Weak allele: brain and discs are small and rudimentary and grow slowly in transplants.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible by aPKC^k06403/aPKC[+]

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible by numb^UAS.Tag:HA/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^ase.neuro/numb^{ΔC.UAS.Tag:HA}

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible by numb^{ΔN.UAS.Tag:MYC}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible by numb^{S2A.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible by numb^{S2D.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

NOT suppressed by

Statement

Reference

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, non-suppressible by numb^{ΔPTB.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has abnormal neuroanatomy | larval stage phenotype, non-suppressible by numb^{ΔAB.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

Suppressor of

Statement

Reference

l(2)gl³³⁴/l(2)gl[+] is a suppressor | partially of visible | dominant phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has intermediate neuronal progenitor | increased number | larval stage phenotype, suppressible by aPKC^k06403/aPKC[+]

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, suppressible by numb^UAS.Tag:HA/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, suppressible by Scer\GAL4^ase.neuro/numb^{ΔC.UAS.Tag:HA}

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, suppressible by numb^{ΔN.UAS.Tag:MYC}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, suppressible by numb^{S2A.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, suppressible by numb^{S2D.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type II neuroblast | increased number | larval stage phenotype, suppressible by aPKC^k06403/aPKC[+]

(Haenfler et al., 2012)

l(2)gl³³⁴ has larval brain & neuroblast | supernumerary phenotype, suppressible by aPKC^k06403

(Lee et al., 2006)

l(2)gl³³⁴ has wing disc phenotype, suppressible by aPKC^k06403/aPKC[+]

(Rolls et al., 2003)

l(2)gl³³⁴ has neuroblast & larval brain & third instar larva phenotype, suppressible by aPKC^k06403/aPKC[+]

(Rolls et al., 2003)

l(2)gl³³⁴ has ventral thoracic disc & epithelial cell phenotype, suppressible by aPKC^k06403/aPKC[+]

(Rolls et al., 2003)

l(2)gl³³⁴ has leg disc phenotype, suppressible by aPKC^k06403/aPKC[+]

(Rolls et al., 2003)

l(2)gl³³⁴ has dorsal mesothoracic disc & epithelial cell phenotype, suppressible by aPKC^k06403/aPKC[+]

(Rolls et al., 2003)

l(2)gl³³⁴/l(2)gl⁴ has embryonic/larval brain | third instar larval stage phenotype, suppressible | partially by aPKC^k06403/aPKC[+]

(Rolls et al., 2003)

l(2)gl³³⁴ has phenotype, suppressible by Dpse\l(2)gl^+t12.2

(Torok et al., 1993)

NOT suppressed by

Statement

Reference

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, non-suppressible by numb^{ΔPTB.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

l(2)gl³³⁴/l(2)gl³⁶⁴⁴ has type I neuroblast | increased number | larval stage phenotype, non-suppressible by numb^{ΔAB.UAS.Tag:HA}/Scer\GAL4^ase.neuro

(Haenfler et al., 2012)

Suppressor of

Statement

Reference

l(2)gl³³⁴/l(2)gl[+] is a suppressor | partially of eye phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

l(2)gl³³⁴/l(2)gl[+] is a suppressor | partially of ommatidium phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

l(2)gl³³⁴/l(2)gl[+] is a suppressor | partially of retina phenotype of Fmr1^sev.PW

(Zarnescu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

aPKC^k06403/+ suppresses the increased number of type II neuroblasts and intermediate neural progenitor cells seen in l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains.

The formation of supernumerary type I neuroblasts which is seen in l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains is suppressed by expression of either numb^{Scer\UAS.T:Ivir\HA1}, numb^{ΔN.Scer\UAS.hs.T:Hsap\MYC}, numb^{S2A.Scer\UAS.T:Ivir\HA1}, numb^{S2D.Scer\UAS.T:Ivir\HA1} or numb^{ΔC.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ase.neuro, but is not suppressed by expression of either numb^{ΔPTB.Scer\UAS.T:Ivir\HA1} or numb^{ΔAB.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ase.neuro.

The formation of supernumerary type I neuroblasts which is seen in l(2)gl³³⁴/l(2)gl³⁶⁴⁴ larval brains is suppressed by co-expression of numb^{ΔC.Scer\UAS.T:Ivir\HA1}, but is not suppressed by co-expression of either numb^{ΔAB.Scer\UAS.T:Ivir\HA1} or numb^{ΔPTB.Scer\UAS.T:Ivir\HA1}.

(Haenfler et al., 2012)

The brains of l(2)gl³³⁴ raps^P62 double mutant larvae contain large numbers of ectopic, proliferating, self-renewing neuroblasts.

(Lee et al., 2006)

l(2)gl³³⁴ aPKC^k06403/l(2)gl³³⁴ + larvae exhibit essentially normal leg and wing imaginal disc morphology including disc size, columnar cell shape and an approximately normal apical membrane domain, at the early third instar stage. The increase in L3 larval brain volume seen in l(2)gl³³⁴/l(2)gl⁴ animals is partially suppressed by aPKC^k06403/+. The increase in neuroblast number seen in homozygous l(2)gl³³⁴ third larval instar brains is partially suppressed by aPKC^k06403/+.

(Rolls et al., 2003)

Xenogenetic Interactions

Statement

Reference

Mutant phenotype completely rescued by Dpse\l(2)gl^+t12.2.

(Torok et al., 1993)

Complementation and Rescue Data

Fails to complement

Rescued by

l(2)gl³³⁴/l(2)gl⁴ is rescued by Scer\GAL4^hh.PU/l(2)gl^UASp.GFP

(Bell et al., 2015)

l(2)gl³³⁴ is rescued by l(2)gl^+t13.1

(Opper et al., 1987)

Partially rescued by

l(2)gl³³⁴/l(2)gl⁴ is partially rescued by Scer\GAL4^hh.PU/l(2)gl^{UAS.Tag:Myr(Unk)}

(Bell et al., 2015)

l(2)gl³³⁴/l(2)gl⁴ is partially rescued by l(2)gl^ASA.UAS.GFP/Scer\GAL4^hh.PU

(Bell et al., 2015)

Comments

Expression of Scer\GAL4^hh.PU>l(2)gl^{Scer\UAS.P\T.T:Avic\GFP} rescues the cell polarity defects in l(2)gl⁴/l(2)gl³³⁴ mutants.

Expression of Scer\GAL4^hh.PU>l(2)gl^{ASA.Scer\UAS.T:Avic\GFP} rescues the cell polarity defects in l(2)gl⁴/l(2)gl³³⁴ mutants and l(2)gl⁴ mutant somatic clones, but reveals spindle orientation defects.

Expression of Scer\GAL4^hh.PU>l(2)gl^{Scer\UAS.T:Uuuu\Myr-Unk} rescues the cell polarity defects in l(2)gl⁴/l(2)gl³³⁴ mutants and l(2)gl⁴ mutant somatic clones, but reveals spindle orientation defects.

(Bell et al., 2015)

Mutant phenotype can be completely rescued by l(2)gl^+t13.1.

(Opper et al., 1987)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Isolated from: Uman', Ukraine population.

(Mechler et al., 1985)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

334

(Golubovsky et al., 2001, Sokolova and Golubovsky, 1979)

l(2)gl334

(Gateff, 1978)

l(2)gl³³⁴

(Zarnescu et al., 2005)

l(2)gl^U334

(Farkas and Mechler, 2000, Mechler et al., 1985)

l(2)glU334

(Gateff and Mechler, 1989, Opper et al., 1987)

lgl³³⁴

(Bell et al., 2015, Haenfler et al., 2012, Atwood and Prehoda, 2009, Lee et al., 2006, Lee et al., 2006, Albertson and Doe, 2003, Rolls et al., 2003, Peng et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (19)

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