FlyBase Allele Report: Dmel\lz[3]

General Information

Symbol

Dmel\lz³

Species

D. melanogaster

Name

FlyBase ID

FBal0011764

Feature type

allele

Associated gene

Dmel\lz

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Riesgo-Escovar et al., 1997, Riesgo-Escovar et al., 1997, Green and Green, 1956)

Progenitor genotype

Cytology

Description

Deletion of 358bp causing a frame shift, along with the insertion of 19bp.

(Jackson Behan et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

X:9,302,740..9,303,097 [+]

Comment:

Deletion of 358bp and insertion of 19bp leading to a frameshift.

Inserted_sequence:

TATAATATAATAATTTACT

(Jackson Behan et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe commissure

(Stocker and Gendre, 1988)

adult antennal nerve

(Stocker and Gendre, 1988)

antennal lobe glomerulus

(Jhaveri and Rodrigues, 2002)

antennal lobe glomerulus DL2

(Stocker and Gendre, 1988)

antennal lobe glomerulus V

(Jhaveri and Rodrigues, 2002, Stocker and Gendre, 1988)

antennal lobe glomerulus VL2

(Stocker and Gendre, 1988)

basiconic sensillum

(Goulding et al., 2000)

basiconic sensillum of antennal segment 3

(Riesgo-Escovar et al., 1997, Ray and Rodrigues, 1995, Stocker et al., 1993, Venard and Stocker, 1991, Stocker and Gendre, 1989, Stocker and Gendre, 1988)

coeloconic sensillum of antennal segment 3

(Riesgo-Escovar et al., 1997, Venard and Stocker, 1991, Stocker and Gendre, 1988)

eye

(Siddall et al., 2003, Batterham et al., 1996, Green and Green, 1956)

eye-antennal disc | P-stage

(Lienhard and Stocker, 1991)

eye disc

(Siddall et al., 2003)

fascicle & antennal segment 3

(Jhaveri et al., 2000)

maxillary palp basiconic sensillum

(Riesgo-Escovar et al., 1997, Venard and Stocker, 1991, Stocker and Gendre, 1988)

ommatidium

(Batterham et al., 1996)

ovary

(Green and Green, 1956)

pigment cell

(Green and Green, 1956)

sensillum coeloconicum & antennal segment 3

(Jhaveri et al., 2000)

spermatheca

(Green and Green, 1956)

third segment of antenna

(Stocker et al., 1993, Stocker and Gendre, 1988)

trichoid sensillum

(Goulding et al., 2000, Stocker and Gendre, 1988)

trichoid sensillum of antennal segment 3

(Ray and Rodrigues, 1995, Stocker et al., 1993, Venard and Stocker, 1991)

unguis

(Green and Green, 1956)

Detailed Description

Statement

Reference

lz³ adults have a "spectacle" eye phenotype. Ectopic cell death is seen in third larval instar eye discs (this phenotype is variable).

(Siddall et al., 2003)

Mutants lack antennal glomerulus V and some central glomeruli are poorly formed.

(Jhaveri and Rodrigues, 2002)

The number of sensilla basiconica is reduced in lz³ mutants. The number of sensilla trichodea is approximately 50% of wild type.

(Goulding et al., 2000)

Approximately 300 of the total 450 sensilla coeloconicum normally seen in the third antennal segment and a subsequent reduction of thickness is seen in all three fascicles. Fascicle 2 is the most significantly affected. However the fascicular pattern is comparable to wild-type and the glial cell numbers and positions are nor significantly affected.

(Jhaveri et al., 2000)

The amplitude of the physiological response of the maxillary palp to ethyl acetate, benzaldehyde, propionic acid, methyl-cyclohexanol, 3-octanol, 1-butanol and acetone is reduced compared to wild-type. This phenotype is partly rescued if the flies are carrying Dp(1;Y)y⁺lz⁺. The size and shape of the maxillary palps is normal, although approximately 60% of the basiconic sensilla of the maxillary palp are reduced in size, altered in shape and/or lacking in pores. 8% of the maxillary palp basiconic sensilla are duplicated. This phenotype is partly rescued if the flies are carrying Dp(1;Y)y⁺lz⁺; only 9% of the maxillary palp basiconic sensilla are abnormal in lz³/Dp(1;Y)y⁺lz⁺ flies.

(Riesgo-Escovar et al., 1997)

The basiconic sensilla of the antenna are missing in lz³ flies. The sensilla trichodea of the antenna appear normal. Many antennal coeloconic sensilla appear abnormal, having projections of varying lengths which are not always straight and may appear splayed. Duplication of the antennal coeloconic sensilla is also seen. lz³/Dp(1;Y)y⁺lz⁺ flies have large and small basiconic sensilla on the antenna, and many antennal coeloconic sensilla appear normal. The amplitude of the physiological response of the antenna to ethyl acetate, propionic acid, 3-octanol, benzaldehyde, acetaldehyde and acetone is reduced compared to wild-type. This phenotype is at least partially rescued if the flies are also carrying Dp(1;Y)y⁺lz⁺.

(Riesgo-Escovar et al., 1997)

Homozygotes have a total lack of ommatidial structure in the eye. Pigment is dispersed as a ring around the edge of the eye ("spectacle" phenotype). No fenestrated membrane is visible.

(Batterham et al., 1996)

Females rarely accept courting males and exhibit an unusually high rate of spontaneous ovulation.

(Fuyama, 1995)

Antennae lack all basiconic and trichoid sensilla.

(Ray and Rodrigues, 1995)

The antennal funiculus is reduced in size in hemizygous males. All large and small basiconic sensilla on the antennal funiculus are missing, and the number of trichoid sensilla on the antennal funiculus is reduced. The number of coeloconic sensilla on the antennal funiculus is normal. The density of the coeloconic sensilla on the antennal funiculus is increased and the density of the trichoid sensilla on the antennal funiculus is decreased compared to wild-type.

(Stocker et al., 1993)

The onset of antennal disc eversion is delayed in lz³ mutant pupae. After eversion, no neurons can be seen at the t3 cluster site. The nerve originating in the arista does not fuse with one of the two remaining funicular nerves.

(Lienhard and Stocker, 1991)

Mutants lack antennal basiconic sensilla, have a marked deficit in trichoid sensilla and small increase in coeloconic sensilla. Basiconic-like sensilla on the maxillary palps are only partially eliminated. In a 'jump assay' mutants do not jump in response to strong olfactory stimuli, undiluted organic chemicals. Flies are sluggish and tend to become motionless after short periods of time. Strong concentrations of chemicals elicit a strong response, flies start immediately to move, the walk response. Elimination of the maxillary palp sensilla by palp ablation does not change the response. Ethanol is capable of evoking a walk response at concentrations lower than 10%. EAG responses to alcohols (but not to esters) are markedly lower than wild type.

(Venard and Stocker, 1991)

Lacks basiconic antennal sensilla. Courtship vigor is similar to wild type under red light but is much lower in normal light conditions. When maxillary palps are surgically removed males court mated females and immature males. lz³ females are much less active in a courtship situation than wild type females, comparisons with lz³⁷ females demonstrate this is not due to an olfactory defect.

(Stocker and Gendre, 1989)

The basiconic sensilla of the funiculus are completely missing in lz³ flies. The number of trichoid and pit sensilla are reduced. The funiculus is smaller, but the number of coeloconic sensilla is significantly enlarged. Basiconica-like sensilla on the maxillary palps are relatively unaffected, with the shaft occasionally being shortened. The number of nerve fibres in the antennal nerve and antennal commissure is significantly reduced. The antennal glomeruli V are almost always missing. Antennal glomeruli VL2 and DL2 usually appear fused.

(Stocker and Gendre, 1988)

Eyes are greatly reduced in size and have a smooth surface in homozygous flies. Red eye pigment is sparse and confined primarily to the margin of the eye. Tarsal claws are vestigial. The spermathecae and pars ovariae are absent in homozygous females.

(Green and Green, 1956)

Eye size sharply reduced; surface smooth. Optic disk of mature larva and prepupa two-thirds normal size (Chen, 1929). Red pigment greatly reduced; color yellowish brown, cream colored in combination with v. Tarsal claws vestigial. Homozygous females lack parovaria and spermathecae and are sterile; lz³/+ females lack parovaria and many have abnormal spermathecae (Anderson, 1945). Unaffected by su(f)⁶ (Schalet, 1970). RK1. glossy; tarsal structures reduced or absent; spermathecae absent; parovaria absent female-sterile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hs.PB, lz³ has visible | recessive phenotype, suppressible by amos^UAS.cGa

(Goulding et al., 2000)

Other

Statement

Reference

Antp[+]/Antp⁶, lz³ has abnormal neuroanatomy phenotype

(Roy et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hs.PB, lz³ has basiconic sensillum phenotype, suppressible | partially by amos^UAS.cGa/Scer\GAL4^hs.PB

(Goulding et al., 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.PB, lz³ has basiconic sensillum phenotype, non-suppressible by ato^UAS.cJa

(Goulding et al., 2000)

lz³ has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Suppressor of

Statement

Reference

lz³ is a suppressor of glial cell | ectopic & antennal disc phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Dll-981

(Sen et al., 2004)

Other

Statement

Reference

Antp[+]/Antp⁶, lz³ has antennal lobe glomerulus phenotype

(Roy et al., 2007)

Scer\GAL4^hs.PB, ato^UAS.cJa, lz³ has coeloconic sensillum phenotype

(Goulding et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The olfactory glomeruli are poorly defined in lz³, Antp⁶/+ animals. The architecture of the CSD interneuron (CSDn), however, closely resembles that of the wild-type.

(Roy et al., 2007)

When BacA\p35^Scer\UAS.cHa is driven by Scer\GAL4^Dll-981 in the antennal disc of lz³/Y mutants, a complete suppression of the ectopic glial cell phenotype is seen.

(Sen et al., 2004)

The number of sensilla trichodea in lz³ mutants is further reduced, to 24% of wild type, if one copy of the chromosomal region containing amos is also removed. The reduction in number of sensilla basiconica seen in lz³ flies is partially rescued by expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^hs.PB. The reduction in number of sensilla basiconica seen in lz³ flies is not rescued by expression of ato^Scer\UAS.cJa under the control of Scer\GAL4^hs.PB even though the number of sensilla coeloconica is increased. lz³; ato¹/ato¹ double mutants show a complete absence of sensilla basiconica and coeloconica, as expected from loss of lz and ato functions respectively.

(Goulding et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

lz^50e

(Batterham et al., 1996)

lz^77a7

(Batterham et al., 1996)

Partially complements

lz³⁷

(Stocker et al., 1993)

Fails to complement

lz^y4

(Batterham et al., 1996)

lz^g

(Batterham et al., 1996)

lz^48f

(Batterham et al., 1996)

lz⁴⁶

(Batterham et al., 1996)

lz³⁶

(Batterham et al., 1996)

lz¹

(Batterham et al., 1996)

lz^pm2

(Batterham et al., 1996)

lz^L

(Batterham et al., 1996)

lz^86b

(Batterham et al., 1996)

lz^mr2

(Batterham et al., 1996)

lz^pm1

(Batterham et al., 1996)

lz^ts1

(Stocker et al., 1993)

lz^K

(Stocker et al., 1993)

lz^g

(Stocker et al., 1993)

lz^50e

(Stocker et al., 1993)

lz¹

(Stocker et al., 1993)

lz^77a7

(Stocker et al., 1993)

lz^b

(Stocker et al., 1993)

lz^L

(Stocker et al., 1993)

lz^38.71

(Stocker et al., 1993)

lz^spm2a

(Stocker et al., 1993)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

105709

Notes on Origin

Discoverer

Bridges, 14th Feb. 1922.

Comments

Clonal analysis suggests that the lack of antennal glomeruli V in lz³ is due to the lack of afferents from antennal basiconic sensilla.

(Stocker and Gendre, 1988)

Mutation affects early steps in sensillum development and many of the founder cells fail to form. Those that are present proceed to form mature olfactory sensilla.

(Ray and Rodrigues, 1995)

lz³ maps to the glossy sublocus of lz.

(Batterham et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gly³

lz³

(Roy et al., 2007, Batterham and MacKechnie, 1980)

Name Synonyms

glossy-3

Secondary FlyBase IDs

References (32)

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