FlyBase Allele Report: Dmel\ovo[D1]

General Information

Symbol

Dmel\ovo^D1

Species

D. melanogaster

Name

FlyBase ID

FBal0013375

Feature type

allele

Associated gene

Dmel\ovo

Associated Insertion(s)

Carried in Construct

Also Known As

ovo^D, Fs(1)K1237

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Mevel-Ninio et al., 1996, Chou et al., 1993, Lindsley and Zimm, 1992, Oliver et al., 1990)

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992)

Progenitor genotype

Cytology

Description

Mutation creates a new in frame AUG codon in the open reading frame in exon 2.

(Mevel-Ninio et al., 1996)

Nucleotide substitution: A1282T.

(Mevel-Ninio et al., 1996)

Amino acid replacement: K79M.

(Mevel-Ninio et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:5,064,663..5,064,663 [+]

Nucleotide change:

A5064663T

Reported nucleotide change:

A1282T

Amino acid change:

K208M | ovo-PB; K79M | ovo-PC; K208M | ovo-PE

Reported amino acid change:

K79M

(Mevel-Ninio et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The pH of the fecal output of mutant females is more similar to that of wild-type virgin males than to that of wild-type virgin females.

Wild-type mated females often excrete fecal deposits which are extremely concentrated, referred to as reproductive oblong deposits (RODs), compared to the fecal deposits of wild-type males and wild-type virgin females. Mated ovo^D1 females show reduced ROD production compared to wild-type mated females.

(Cognigni et al., 2011)

Ovaries from heterozygous ovo^D1 females produce only very rarely egg chambers, which then only survive until stages 2-3 of oogenesis.

(Vazquez-Pianzola et al., 2011)

ovo^D1 flies feed more than control flies. This is because they increase the volume of intake per proboscis extension, rather than by feeding at a greater frequency.

(Wong et al., 2009)

ovo^D1 mutant germ lines exhibit growth arrest after stage 4 resulting in small stage 6 egg chambers, which later degenerate.

(Wang and Riechmann, 2007)

Heterozygous females have an extended life-span compared to controls.

(Clancy et al., 2001)

Heterozygous adult females have atrophied ovaries containing some germ cells but lacking vitellogenic egg chambers. Wild-type clones made in the ovaries of a ovo^D1 female, are significantly larger than clones of heterozygous mutant cells. This size difference is greater in clones initiated at 48 hours after egg laying (AEL) than 2 hours AEL.

(Extavour and Garcia-Bellido, 2001)

The frequency of gypsy insertion into the ovo locus in a flam permissive background is higher in ovo^D1/+ females than in wild-type females. gypsy insertions in ovo^D1 females occur during most stages of germ-line development, in contrast to wild-type females where insertions occur only in late stages.

(Labrador and Corces, 2001)

Heterozygous females have small ovaries bearing only early egg chambers.

(Andrews et al., 2000)

Oogenesis stops at about stage 4 in heterozygous females.

(Sahut-Barnola and Pauli, 1999)

Females carrying ovo^D1 in a wild-type or ovo^-/+ background show dominant female sterility. Heterozygous females contain no mature eggs.

(Andrews et al., 1998)

In heterozygous females oogenesis is arrested prior to or at stage 4.

(Mevel-Ninio et al., 1996)

Oogenesis in heterozygous females is mainly arrested prior to stage 4.

(Granadino et al., 1992)

Germ-cell arrest.

(Lindsley and Zimm, 1992)

Heterozygous females do not lay eggs, egg chambers form but degenerate before vitellogenic stages.

(Mevel-Ninio et al., 1991)

The egg chambers of heterozygous females fail to initiate vitellogenesis, and no eggs are laid. The soma is wild-type. Females heterozygous for both ovo^D1 and snf¹ have germ cells with a male-like morphology.

(Oliver et al., 1990)

Mutant gene activity works in opposition to wild type activity. Viable. Male germ line is fertile, female germ line has no vitellogenic egg chambers.

(Oliver et al., 1987)

The presence of ovo^D1 has no effect on the dl¹ phenotype.

(Campos-Ortega, 1983)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

ovo^D1 has long lived | dominant phenotype, non-enhanceable by chico¹

(Clancy et al., 2001)

NOT Enhancer of

Statement

Reference

ovo^D1 is a non-enhancer of long lived | dominant phenotype of chico¹

(Clancy et al., 2001)

Suppressor of

Statement

Reference

ovo^D1 is a suppressor of abnormal cell shape | somatic clone phenotype of sqh^AX3

(Wang and Riechmann, 2007)

ovo^D1 is a suppressor of abnormal cytokinesis | somatic clone phenotype of sqh^AX3

(Wang and Riechmann, 2007)

Phenotype Manifest In

Suppressor of

Statement

Reference

ovo^D1 is a suppressor of epithelium | somatic clone phenotype of sqh^AX3

(Wang and Riechmann, 2007)

ovo^D1 is a suppressor of follicle cell | somatic clone phenotype of sqh^AX3

(Wang and Riechmann, 2007)

ovo^D1 is a suppressor of egg chamber | somatic clone phenotype of sqh^AX3

(Wang and Riechmann, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Wild-type mated females often excrete fecal deposits which are extremely concentrated, referred to as reproductive oblong deposits (RODs), compared to the fecal deposits of wild-type males and wild-type virgin females. ovo^D1 females which have been mated to Acp70A⁰/Df(3L)Δ130 males do not produce RODs.

(Cognigni et al., 2011)

The deleterious effect on female lifespan of expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 is suppressed by ovo^D1.

The deleterious effect on female lifespan of expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 is suppressed by ovo^D1.

The deleterious effect on female lifespan of expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 is partially suppressed by ovo^D1.

(Tricoire et al., 2009)

sqh^AX3 follicle cell clones maintain their rectangular shape when cyst growth is blocked in ovo^D1 mutants, whereas sqh^AX3 cells are deformed when the cyst grows. The cyst bulges out underneath the sqh^AX3 clones only in the wild-type background, not in ovo^D1 mutant cysts.

(Wang and Riechmann, 2007)

ovo^D1 chico¹ double heterozygotes live as long as chico¹/+ single heterozygotes and significantly longer than ovo^D1/+ single heterozygotes.

(Clancy et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Not rescued by

ovo^D1 is not rescued by ovo^S-7.2

(Mevel-Ninio et al., 1991)

Comments

ovo^S-7.2 cannot rescue the sterility of heterozygous females.

(Mevel-Ninio et al., 1991)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

1309

ovo^D1 v²⁴/C(1)DX, y¹ w¹ f¹

1813

w^* ovo^D1 v²⁴ P{FRT(w^hs)}101/C(1)DX, y¹ f¹; P{hsFLP}38

Kyoto

106273

ovo^D1 v²⁴/C(1)DX, y¹ w¹ f¹

106517

w^* ovo^D1 v²⁴ P{FRT(w^hs)}101/C(1)DX, y¹ f¹/Y; P{hsFLP}38

Notes on Origin

Discoverer

Komitopoulou.

(Lindsley and Zimm, 1992)

Comments

The dominant ovo^D1 mutation has been reverted to a recessive loss of function mutation by insertion of a gypsy element.

(Mevel-Ninio et al., 1991)

The ovo^D1 allele has been cloned and transposed to several regions of the genome in P element vectors to facilitate the generation of germ line mosaics for autosomal mutations.

(Chou et al., 1993)

Parental strain: fs(1)A273.

(Mevel-Ninio et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Fs(1)K1237

(Mathe et al., 1998, Szabad, 1998, Campos-Ortega, 1983)

Ovo^D1

(Fowler et al., 2022, Biehs et al., 2010)

Ovo^D

(Perrimon et al., 1986)

fs(1)K1237

(Perrimon et al., 1986)

ovo^D1

(Yu et al., 2023, Boehm et al., 2022, Rodríguez-Muñoz et al., 2022, van Dam et al., 2020, Ray et al., 2019, Genenncher et al., 2018, Fedina et al., 2017, Reiff et al., 2015, Vilmos et al., 2013, Cognigni et al., 2011, Popodi et al., 2010-, Tricoire et al., 2009, Wong et al., 2009, Labrador et al., 2008, Wang and Riechmann, 2007)

ovo^D

(Dey et al., 2023, Ho et al., 2022, Soffers et al., 2021, Catterson et al., 2018, Starble and Pokrywka, 2018, Beaucher et al., 2007, Shapiro and Anderson, 2006, Aigaki et al., 2002, Cox et al., 2001, Yamamoto and Nakano, 1999, Fuyama, 1994)

ovoD1

(Torres-Zelada et al., 2022)

ovoD¹

(Vazquez-Pianzola et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (72)

Report Sections

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