Deletion of nucleotide 2271A.
Small deletion or point mutation.
A one base deletion of an A nucleotide.
The addition of L-DOPA to food ingested by ple2 heterozygotes significantly improves food intake.
Flies expressing pleFS.Scer\UAS in a ple2 background exhibit reduced walking speed compared to wild-type. Similarly, they cover a shorter distance in a 15 minute period. These flies also respond poorly to mechanical shock (that induces climbing in wild-type flies).
Flies expressing pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) are overall less active and possibly sleep more frequently than controls. In addition, sleeping pleFS.Scer\UAS; ple2 flies respond less frequently to mild and moderate mechanical stimuli, whereas response to strong stimuli is comparable to controls.
Flies expressing pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) show no response to 3-iodotyrosine (whereas controls exhibit decreased activity). In contrast, caffeine is found to decrease resting periods in these flies.
Flies expressing pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) do not show any avoidance to shock-associated odors indicating impaired adversive olfactory learning. Interestingly, these flies show an inverse tendency to choose the shock-associated odor during testing. This effect is significant 2 hours after training.
Treatment of flies expressing pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) with the DA precursor L-DOPA significantly improves their negative geotaxis behavior and olfactory learning.
Flies expressing pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) show no significant response to sucrose. The proboscis extension reflex appears normal in these mutants however, indicating that sugar perception and reflex appetitive reactions are preserved. Quantification of food intake suggests that these flies eat one-third as much as controls, demonstrating that they are markedly hypophagic. The addition of L-DOPA significantly improves this phenotype.
Flies expressing pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) appear to show a lack of phototaxis despite displaying normal electroretinograms and optomotor responses.
Flies expressing pleScer\UAS.cFa or pleFS.Scer\UAS in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) display slightly less accurate visual orientation than wild-type. Similarly, using the detour paradigm, both pleScer\UAS.cFa; ple2 and pleFS.Scer\UAS; ple2 flies demonstrate a roughly wild-type spatial orientation memory.
ple2 flies expressing either pleScer\UAS.cFa or pleFS.Scer\UAS under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL exhibit normal adult life expectancy.
Flies expressing pleScer\UAS.cFa in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) demonstrate associative memory similar to wild-type, indicating normal olfactory learning.
Flies expressing pleScer\UAS.cFa in a ple2 background (under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL) demonstrate partial rescue of ple2 phototactic behavior.
Heterozygous embryos have ectopically migrating tracheal branches and tracheal cells. The dorsal trunk is often convoluted.
Heterozygotes have normal locomotor activity at 25oC.
ple+t8 rescues mutant embryos to viable adults. Some adults die attempting to free themselves from the pupal case, the greatest lethality occurs during the pharate adult stage. Adults that do eclose are paler than their ple+ siblings and they appear weaker in their movements with a tendency to drown in the food medium. Rescued larvae are normal in their response to both light and NaCl.
TorsinKO13, ple2/ple[+] has abnormal locomotor behavior | third instar larval stage phenotype
Double heterozygous TorsinKO13/+; ple2/+ female larvae show a slight but significant reduction in peristaltic frequency relative to either single heterozygote.
ple2 is rescued by Scer\GAL4ple.PF/pleUAS.cFa/Scer\GAL4Ddc.PL
ple2 is rescued by Scer\GAL4ple.PF/pleFS.UAS/Scer\GAL4Ddc.PL
ple2 is rescued by Scer\GAL4ple.PF/pleUAS.cFa
ple2 is rescued by Scer\GAL4elav-C155/ple1.UAS
ple2 is rescued by Scer\GAL4C1003/ple1.UAS
ple2 is rescued by Scer\GAL4ple.PF/ple2.UAS
ple2 is rescued by Scer\GAL4elav-C155/ple2.UAS
ple2 is rescued by Scer\GAL4C1003/ple2.UAS
ple2 is not rescued by Scer\GAL4ple.PF/pleFStrunc.UAS/Scer\GAL4Ddc.PL
ple2 is not rescued by Scer\GAL4ple.PF/ple1.UAS
ple2 is not rescued by ple1.UAS/Scer\GAL4ple.5'
ple2 is not rescued by Scer\GAL4ple.5'/ple2.UAS
Expression of pleScer\UAS.cFa under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL fully rescues (to the adult stage) the embryonic lethality normally found in ple2 mutants.
Expression of pleFStrunc.Scer\UAS under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL fails to rescue the embryonic lethality normally found in ple2 mutants.
Expression of pleFS.Scer\UAS under the control of both Scer\GAL4ple.PF and Scer\GAL4Ddc.PL fully rescues (to the adult stage) the embryonic lethality normally found in ple2 mutants.
ple2-induced embryonic lethality is not rescued by the presence of pleΔ1g.
The presence of pleΔ2g transgene either significantly rescues ple2 embryonic lethality or full pupal rescue. Few rescued adults are observed with pleΔ2g, possibly because of a limiting expression level of the transgene at this stage.
Expression of pleScer\UAS.cFa in DA cells under the control of Scer\GAL4ple.PF leads to full pupal rescue of ple2.
Expression of pleΔ1.Scer\UAS in DA cells under the control of Scer\GAL4ple.PF fails to rescue the embryonic lethality seen in ple2 mutants.
Expression of pleΔ2.Scer\UAS in DA cells under the control of Scer\GAL4ple.PF leads to full pupal rescue of ple2.
Neuronal expression of pleΔ1.Scer\UAS under the control of Scer\GAL4elav-C155 or Scer\GAL4C1003 results in significant rescue of ple2 larvae. Examination of the rescued ple2 larvae reveals that their mouth hooks and denticle belts are pigmented. Rare adult escapers are observed exhibiting a weak and pale cuticle and much reduced viability.
Neuronal expression of pleΔ2.Scer\UAS under the control of Scer\GAL4elav-C155 or Scer\GAL4C1003 results in significant rescue of ple2 larvae.
Expression of pleΔ1.Scer\UAS or pleΔ2.Scer\UAS under the control of Scer\GAL4ple.5' does not rescue the embryonic lethality seen in ple2 mutants.
ple+t8 rescues mutant embryos to viable adults.