FlyBase Allele Report: Dmel\pum[1]

General Information

Symbol

Dmel\pum¹

Species

D. melanogaster

Name

FlyBase ID

FBal0014072

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

Also Known As

pum^ET1

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Barker et al., 1992)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Ye et al., 2004)

female sterile

(Forbes and Lehmann, 1998)

lethal | maternal effect

(Barker et al., 1992)

lethal - all die during embryonic stage | maternal effect | recessive

(Parisi and Lin, 1999)

some die during embryonic stage | recessive | maternal effect | recessive

(Parisi and Lin, 1999)

sterile (with pum⁰²⁰⁰³)

(Ye et al., 2004)

visible (with pum³)

(Kim et al., 2012)

visible (with pum⁰¹⁶⁸⁸)

(Kim et al., 2012)

visible (with pum^Msc)

(Kim et al., 2012)

Phenotype Manifest In

dendritic arborising neuron & dendrite | somatic clone

(Ye et al., 2004)

female germline stem cell | somatic clone

(Gilboa and Lehmann, 2004)

germarium (with pum⁹)

(Szakmary et al., 2005)

germline cell

(Forbes and Lehmann, 1998)

macrochaeta | ectopic (with pum^Msc)

(Kim et al., 2012)

metathoracic tergum (with pum^Msc)

(Kim et al., 2012)

mouth hook (with pum⁶⁸⁰)

(Gamberi et al., 2002)

ovariole

(Forbes and Lehmann, 1998)

ovariole (with pum⁹)

(Szakmary et al., 2005)

ovariole | germline clone | maternal effect

(Parisi and Lin, 1999)

wing vein | ectopic (with pum³)

(Kim et al., 2012)

wing vein | ectopic (with pum⁰¹⁶⁸⁸)

(Kim et al., 2012)

wing vein | ectopic (with pum^Msc)

(Kim et al., 2012)

Detailed Description

Statement

Reference

Trans-heterozygotes of pum¹/pum⁰¹⁶⁸⁸, pum¹/pum^Msc and pum¹/pum³ display extra wing veins. The penetrance of the pum¹/pum⁰¹⁶⁸⁸ phenotype is incomplete.

Trans-heterozygotes of pum¹/pum^Msc display extra macrochaetae on their notum. The penetrance of the phenotype is incomplete.

(Kim et al., 2012)

pum¹/pum⁹ ovarioles are characterised by germline-empty germaria and an egg chamber.

(Szakmary et al., 2005)

Homozygous germ line stem cell (GSC) clones are rapidly lost from the adult ovary; GSC loss is seen as early as 4 days after clone induction, and by the 6th or 7th day after clone induction, most ovarioles do not contain a mutant GSC.

(Gilboa and Lehmann, 2004)

Third larval instar class I or class II dendritic arborization neurons which are mutant for pum¹ (generated using the MARCM technique) do not show an alteration in dendrite morphology, as assessed by total length of dendrites and quantitation of dendritic order. Third larval instar class III dendritic arborization neurons which are mutant for pum¹ (generated using the MARCM technique) have significantly elongated dendritic spikes, but the order of dendrites and the length of major dendritic branches is indistinguishable from wild type. Third larval instar class IV dendritic arborization neurons which are mutant for pum¹ (generated using the MARCM technique) show a defect in their dendrites; incomplete coverage of the epidermis is seen in about 15% of the mutant neurons (wild-type neurons cover the epidermis in a complete but non-overlapping fashion), due to a reduction of higher-order branches.

(Ye et al., 2004)

98% of pum¹³/pum¹ larvae fail to develop the normal mouth hook projection.

(Gamberi et al., 2002)

Homozygous female germline clones induced at the third larval instar stage produce "differentiated" ovarioles that on average 2.2 +/- 1.5 mature egg chambers. Females containing homozygous female germline clones achieve an oviposition efficiency of 78% (for absolute number of eggs laid by the females). The eggs show posterior patterning defects and fail to hatch.

(Parisi and Lin, 1999)

pum¹/In(3R)Scr^Msc mutant females lay some eggs during their first week of life, by 10 days after eclosion the ovaries are completely depleted of germline. 18 days after eclosion some of the now germline-less germaria appear swollen, by 35 days after eclosion some ovarioles have become massively tumorous. The tumours are somatic, rather than germline in origin. Tumours are also seen in ovarioles that have not lost their germline and not in ovarioles that have. Transplantation of wild type germ cells can rescue the pum ovary phenotype.

(Forbes and Lehmann, 1998)

In embryos derived from pum¹³/pum¹ mothers, the domain of hb protein is expanded in a posterior direction.

(Barker et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

pum¹/pum¹ is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4^109(2)80, nanos^{αTub84B.3'UTR.UAS}

(Ye et al., 2004)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

pum^Msc/pum¹ has ovary phenotype, non-suppressible by cup^unspecified

(Verrotti and Wharton, 2000)

Suppressor of

Statement

Reference

pum¹/pum¹ is a suppressor | partially of dendritic arborising neuron & dendritic tree phenotype of Scer\GAL4^109(2)80, nanos^{αTub84B.3'UTR.UAS}

(Ye et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Most germaria are devoid of germ cells in pum¹ bam^Δ86/pum⁹ bam^Δ86 double mutant females.

(Szakmary et al., 2005)

The reduction in high-order dendritic branches seen in class IV dendritic arborization neurons of third instar larvae expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4⁴⁷⁷ is not as extreme if the larvae are also homozygous for pum¹. Third larval instar class I dendritic arborization neurons of third instar larvae which are mutant for both pum¹ and nos¹⁸ (generated using the MARCM technique) do not have any defects in dendrite morphology. Third larval instar class III dendritic arborization neurons which are mutant for both pum¹ and nos¹⁸ (generated using the MARCM technique) show an increase in the number of long dendritic spikes to a similar extent as that seen in the single mutant class III dendritic arborization neurons. Third larval instar class IV dendritic arborization neurons which are mutant for both pum¹ and nos¹⁸ (generated using the MARCM technique) show incomplete innervation of the epidermal territory in about 18% of neurons, an extent similar to that seen in the single mutant class IV dendritic arborization neurons.

(Ye et al., 2004)

cup^unspecified has no effect on the oogenesis defects associated with pum¹/pum^Msc females.

(Verrotti and Wharton, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

pum⁰¹⁶⁸⁸

(Parisi and Lin, 1999)

Comments

pum⁰¹⁶⁸⁸ partially complements the embryonic lethality of the maternal effect allele pum¹, resulting in viable yet sterile progeny.

(Parisi and Lin, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pum¹

(Kim et al., 2012)

pum^ET-1

(Reichardt et al., 2018)

pum^ET1

(Cairrão et al., 2022, Flora et al., 2018, Marchetti et al., 2014, Harris et al., 2011, Szakmary et al., 2005, Cinnamon et al., 2004, Ye et al., 2004, Gilboa et al., 2003, Verrotti and Wharton, 2000, Parisi and Lin, 1999, Forbes and Lehmann, 1998, Lin and Spradling, 1997, Barker et al., 1992, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (18)

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