FlyBase Allele Report: Dmel\ey[D]

General Information

Symbol

Dmel\ey^D

Species

D. melanogaster

Name

Dominant

FlyBase ID

FBal0028184

Feature type

allele

Associated gene

Dmel\ey

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

Progenitor genotype

Caused by aberration

Dp(2;4)ey^D

Cytology

Description

A piece of the 2nd chromosome causes Dp(2;4)ey^D by inserting into exon 5 of the ey gene replacing 320bp of ey sequence. This causes a truncated ey protein (346 aa) to be produced. Its 32 C terminal amino acids are coded by the inserted sequences of the second chromosome. There is also a 327bp insertion close to the distal end of the duplication - within the ey gene.

(Kronhamn et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | larval stage

(Jacobsson et al., 2009)

lethal | recessive

(Kronhamn et al., 2002, Renfranz and Benzer, 1989, Hochman et al., 1964)

lethal - all die before end of pupal stage

(Jacobsson et al., 2009)

some die during pupal stage

(Jacobsson et al., 2009)

visible

(Jiao et al., 2001)

Phenotype Manifest In

adult head

(Jacobsson et al., 2009, Jiao et al., 2001)

eye

(Jacobsson et al., 2009, Hochman et al., 1964)

eye (with ey²)

(Jang et al., 2003, Kronhamn et al., 2002)

eye (with ey^R)

(Kronhamn et al., 2002)

eye-antennal disc

(Jacobsson et al., 2009, Kronhamn et al., 2002)

head

(Kronhamn et al., 2002)

Detailed Description

Statement

Reference

Homozygous ey^D mutant escaper adults are headless.

Eye-antennal discs dissected from ey^D homozygous larvae exhibit massive cell death.

(Jacobsson et al., 2009)

ey^D/ey² animals have no eyes and is nearly completely penetrant.

(Jang et al., 2003)

About half of ey^D homozygous pharate adults exhibit a headless phenotype, missing all or most structures derived from the eye-antennal disc. ey²/ey^D and ey^R/ey^D animals have small eyes.

(Kronhamn et al., 2002)

Homozygotes show a headless phenotype.

(Jiao et al., 2001)

Geotaxis score no different from Canton S in a Hirsch-type geotaxis maze.

(McMillan and McGuire, 1992)

Adult eyes vary in size from full to nothing, eye disc varies from rudimentary to full-sized, due to precursor cell defects.

(Renfranz and Benzer, 1989)

Eyes are greatly reduced in size.

(Hochman et al., 1964)

Eye¹ and ey^D show an additive effect with respect to eye phenotype; Eye¹/+; ey^D/+ shows more marked eyelessness than either single heterozygote.

(Edmondson, 1952)

RK2. Eyes of heterozygotes small, outline irregular, displaced toward top and rear. Head large, often with duplicated antennae or ocelli. Fully dominant in triplo-4 flies (Sturtevant, 1936). Eye size of B; ey^D/+ males larger than of B alone. Produces extreme phenotype in combination with D. D/+; ey^D/+ almost completely lethal (Sobels, Kruijt and Spronk, 1951). Homozygous lethal; two lethal crises, one during first or second larval instar and the other just prior to or during pupal stage. Cell degeneration observed in optic discs of homozygous second-instar larval (Ransom, 1979). Larvae which are unable to pupate rescuable by injection of α-ecdysone (Arking, 1969). Homozygotes reaching pupal stage lack adult derivatives of eye-antennal discs; adult derivatives are formed by ey^D/ey^D eye-antennal discs transplanted into wild-type hosts; brain present but number of cortical cells severely reduced (Arking, Putnam, and Schubiger, 1975).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ey^D has lethal | pupal stage phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^UAS.cHa

(Jacobsson et al., 2009)

ey^D has increased cell death | larval stage phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^UAS.cHa

(Jacobsson et al., 2009)

ey^D has lethal | pupal stage phenotype, suppressible by toy^UAS.cCa/Scer\GAL4^toy2

(Jacobsson et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

ey^D has adult head phenotype, suppressible by toy^UAS.cCa/Scer\GAL4^toy2

(Jacobsson et al., 2009)

ey^D has eye-antennal disc phenotype, suppressible by toy^UAS.cCa/Scer\GAL4^toy2

(Jacobsson et al., 2009)

ey^D has eye phenotype, suppressible by toy^UAS.cCa/Scer\GAL4^toy2

(Jacobsson et al., 2009)

ey^D has adult head phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^UAS.cHa

(Jacobsson et al., 2009)

ey^D has eye phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^UAS.cHa

(Jacobsson et al., 2009)

ey^D/ey² has eye phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/eyg^UAS.lune

(Jang et al., 2003)

ey^D has eye-antennal disc phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Kronhamn et al., 2002)

ey^D has head phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Kronhamn et al., 2002)

NOT suppressed by

Statement

Reference

ey^D has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ey.PH, ey^D has eye phenotype

(Kronhamn et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 significantly suppresses the lethality seen in ey^D mutants, raising the fraction of homozygous adults from 0.27% to 7.3%. The morphology of compound eyes in these flies is also improved by targeted expression of toy^Scer\UAS.cCa, but never to wild-type proportions. A small eye phenotype is found in 76% of rescued flies.

(Jacobsson et al., 2009)

The addition of BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^ey.PH more than half of the flies are rescued to viable adults that are eyeless, but have developed most other head structures derived from the eye-antennal discs, usually including all three ocelli.

(Kronhamn et al., 2002)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^toy2 significantly increases the survival of toy^hdl homozygous mutants to the adult stage. The head structure defects in these rescued flies, apart from the compound eyes, are also improved.

(Jacobsson et al., 2009)

The addition of BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^ey.PH rescues more than half of the flies to viable adults that are eyeless, but have developed most other head structures derived from the eye-antennal discs, usually including all three ocelli.

(Kronhamn et al., 2002)

Complementation and Rescue Data

Rescued by

ey^D is rescued by ey^UAS.cHa/Scer\GAL4^ey.PH

(Jacobsson et al., 2009)

ey^D is rescued by ey^UAS.cHa

(Jacobsson et al., 2009)

Comments

Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH significantly rescues the lethality seen in ey^D homozygous mutants. The flies that eclose from pupae exhibit smaller than normal heads and a majority of the compound eyes are reduced in size, while the ocelli are normal. The viability of these flies is more efficient at 18[o]C compared to 25[o]C and 29[o]C. The rescue efficiencies of flies carrying both driver and effector constructs or effector construct alone (i.e only ey^Scer\UAS.cHa) are similar.

(Jacobsson et al., 2009)

Dp(2;4)ey^D chromosome is also associated with a dominant gain of function mutation, Alp^eyD (FBal0027957), which is responsible for the leg phenotype previously associated with ey^D.

(Cohen, 1995.4.19)

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type