FlyBase Allele Report: Dmel\eya[2]

General Information

Symbol

Dmel\eya²

Species

D. melanogaster

Name

FlyBase ID

FBal0030759

Feature type

allele

Associated gene

Dmel\eya

Associated Insertion(s)

Carried in Construct

Also Known As

eya^ph

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Bonini et al., 1998, Leiserson et al., 1994)

Progenitor genotype

Cytology

Description

322bp deletion 581bp upstream of the transcription start site of the type I eya transcript.

(Zimmerman et al., 2000)

Small deletion outside the coding region.

(Leiserson et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:6,547,553..6,547,872 [-]

Comment:

comment - Mutation reported as a 322bp deletion but corresponds to a 320 bp region in the 5' UTR of eya on the R6 reference sequence. The mapped sequence boundaries are taken from the sequence in Figure 4.

(Zimmerman et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Fedele et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | adult stage

(Senthilan et al., 2012)

abnormal eclosion rhythm

(McNabb and Truman, 2008)

abnormal neuroanatomy | third instar larval stage (with eya^A188)

(Xiong et al., 2009)

abnormal size | adult stage (with eya³)

(Tian et al., 2019)

abnormal size | adult stage (with eya⁴)

(Tian et al., 2019)

abnormal size | adult stage (with eya^54C2)

(Tian et al., 2019)

abnormal size | adult stage (with eya^cli-IID)

(Tian et al., 2019)

abnormal size | adult stage (with eya^D3)

(Tian et al., 2019)

abnormal size | adult stage (with eya^E1)

(Tian et al., 2019)

abnormal size | adult stage (with eya^E4)

(Tian et al., 2019)

female fertile

(Bonini et al., 1998)

viable

(Tian et al., 2019, Zimmerman et al., 2000, Bonini et al., 1998, Leiserson et al., 1998, Bonini et al., 1997)

visible

(Jin et al., 2013, Jusiak et al., 2012, Liu et al., 2012, Yeh et al., 2012, Mutsuddi et al., 2005)

visible | adult stage

(Tian et al., 2019, Weasner et al., 2016)

visible | adult stage (with eya³)

(Tian et al., 2019)

visible | adult stage (with eya⁴)

(Tian et al., 2019)

visible | adult stage (with eya^54C2)

(Tian et al., 2019)

visible | adult stage (with eya^cli-IID)

(Tian et al., 2019)

visible | adult stage (with eya^cs)

(Tian et al., 2019)

visible | adult stage (with eya^D3)

(Tian et al., 2019)

visible | adult stage (with eya^E1)

(Tian et al., 2019)

visible | adult stage (with eya^E4)

(Tian et al., 2019)

visible | recessive

(Bui et al., 2000, Zimmerman et al., 2000, Bonini et al., 1998, Leiserson et al., 1998, Bonini et al., 1997, Pignoni et al., 1997, Bonini et al., 1993)

Phenotype Manifest In

adult subretinal glial cell

(Choi and Benzer, 1994)

eye

(Tian et al., 2019, Weasner et al., 2016, Jin et al., 2013, Jusiak et al., 2012, Liu et al., 2012, Yeh et al., 2012, Mutsuddi et al., 2005, Bui et al., 2000, Zimmerman et al., 2000, Bonini et al., 1998, Leiserson et al., 1998, Bonini et al., 1997, Pignoni et al., 1997, Choi and Benzer, 1994, Leiserson et al., 1994)

eye (with eya³)

(Tian et al., 2019)

eye (with eya⁴)

(Tian et al., 2019)

eye (with eya^54C2)

(Tian et al., 2019)

eye (with eya^cli-IID)

(Tian et al., 2019)

eye (with eya^cs)

(Tian et al., 2019)

eye (with eya^D3)

(Tian et al., 2019)

eye (with eya^E1)

(Tian et al., 2019)

eye (with eya^E4)

(Tian et al., 2019)

eye | cell autonomous | somatic clone

(Leiserson et al., 1998)

eye disc

(Anderson et al., 2006)

lamina | third instar larval stage (with eya^A188)

(Xiong et al., 2009)

ocellus

(Choi and Benzer, 1994)

ommatidium

(Rayapureddi et al., 2003)

photoreceptor neuron

(Malpel et al., 2002)

presumptive embryonic salivary gland

(Vining et al., 2005)

retina

(Anderson et al., 2006)

Detailed Description

Statement

Reference

eya²/eya² and almost all eya²/eya^cs adults are eyeless. eya²/eya⁴ adults exhibit smaller eyes compared to controls.

eya²/eya⁴ and eya²/eya³ adults exhibit significantly higher number of ommatidia in the eye than eya²/eya^E1, eya²/eya^E4, eya²/eya^54C2 and eya²/eya^D3 adults.

eya²/eya⁴ but not eya²/eya³ adults exhibit significantly higher number of ommatidia in the eye than eya²/eya^cli-IID adults.

eya^cs/eya² adults exhibit more severe eye phenotypes than eya^cs/eya¹, eya^cs/In(2LR)ETD2.2, eya^cs/eya^cs and eya^cs/eya^E(P)10 adults.

(Tian et al., 2019)

Adult eya² homozygous mutants are eyeless.

(Weasner et al., 2016)

Homozygous adults completely lack eyes.

(Jin et al., 2013)

Homozygotes show complete loss of eyes.

(Jusiak et al., 2012)

The eyes do not develop in flies carrying homozygous eya².

(Liu et al., 2012)

Mutant flies show mild but significant defects in the auditory response to sound compared to wild type.

(Senthilan et al., 2012)

Instead of forming an even plexus of growth cones at the lamina, photoreceptor axons in eya^A188/eya² larvae fasciculate aberrantly to produce an irregular pattern of gaps and thickenings in the lamina, with a significant percentage of axon bundles failing to terminate properly at the lamina.

(Xiong et al., 2009)

The eclosion of eya² mutant flies under photoperiod conditions lacks the prominent lights-on response seen in wild-type flies. No significant increases in eclosion are seen regardless of when flies are exposed to the lights-on signal. The eclosion rate is variable and substantial eclosion precedes the lights-on signal, but overall eclosion appears circadian. Flies exhibit normal geotaxis. The time taken for the flies to spread their wings post-eclosion is similar in flies that eclose in the dark and those that eclose in response to the lights-on signal. This is in contrast to controls, in which inflation occurs more quickly in the dark.

(McNabb and Truman, 2008)

eya² mutants have a small eye disc and show a complete loss of the retina.

(Anderson et al., 2006)

Mutants completely lack eye tissue.

(Mutsuddi et al., 2005)

In eya² embryos the distal tip of the salivary gland is not elongated along the anterior-posterior axis like in wild type, but is either oriented ventrally or curled underneath the long axis of the gland.

(Vining et al., 2005)

eya² mutant flies have no ommatidia.

(Rayapureddi et al., 2003)

Homozygous mutant larvae show no glial cell migration defects in the developing eye.

(Hummel et al., 2002)

Third instar larvae lack the developing adult photoreceptors. The dendritic arborisation of the lateral neurons appears normal.

(Malpel et al., 2002)

Homozygotes completely lack eyes, whereas the ocelli are normal.

(Zimmerman et al., 2000)

eya²/eya¹ flies are eyeless. eya²/eya³ flies have eyes that are between 3/4 and less than normal size, and eya²/eya³ females are fertile.

(Bonini et al., 1998)

eya²/eya^E3 flies are viable, and have eyes which range from 1/2 to less than normal size. eya²/eya⁴ flies have eyes which range from 1/2 to less than normal size. Homozygotes are eyeless. Homozygous clones in the eye do not survive. This phenotype is cell autonomous.

(Leiserson et al., 1998)

Homozygotes are completely eyeless. Eyes are restored to up to 3/4 of normal size in eya² flies carrying one copy of eya^Scer\UAS.cBa expressed under the control of either Scer\GAL4^ey.PB, Scer\GAL4^dpp.blk1, Scer\GAL4^T59 or Scer\GAL4^T155.

(Bonini et al., 1997)

Homozygotes are completely eyeless.

(Pignoni et al., 1997)

Displays locomotor activity rhythm with an approximately 24h period.

(Vosshall and Young, 1995)

Migration of the retinal basal glia from the optic stalk to the eye disc does not occur.

(Choi and Benzer, 1994)

Homozygous flies are eyeless. eya²/eya⁴ heterozygous flies have 3/4 sized eyes.

(Leiserson et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

eya^cs/eya² has visible | adult stage phenotype, enhanceable by Pc¹/Pc[+]

(Tian et al., 2019)

NOT Enhanced by

Statement

Reference

eya^E4/eya² has visible | adult stage phenotype, non-enhanceable by Cap-H2^Z3-0019/Cap-H2[+]

(Tian et al., 2019)

eya^E4/eya² has abnormal size | adult stage phenotype, non-enhanceable by Cap-H2^Z3-0019/Cap-H2[+]

(Tian et al., 2019)

Suppressed by

Statement

Reference

eya^E4/eya² has abnormal size | adult stage phenotype, suppressible | partially by Cap-H2^Z3-5163/Cap-H2^Z3-0019

(Tian et al., 2019)

eya^cs/eya² has visible | adult stage phenotype, suppressible | partially by E(z)[+]/E(z)⁶⁰

(Tian et al., 2019)

eya^E4/eya² has visible | adult stage phenotype, suppressible | partially by Cap-H2^Z3-5163/Cap-H2^Z3-0019

(Tian et al., 2019)

eya² has visible | adult stage phenotype, suppressible by so^{VP16.UAS.FL.NT}/Scer\GAL4^ey.PU

(Weasner et al., 2016)

eya² has visible | recessive phenotype, suppressible by Scer\GAL4^ey.PB/Mmus\Eya2^UAS.cBa

(Bui et al., 2000, Bonini et al., 1997)

eya² has visible | recessive phenotype, suppressible by Mmus\Eya1^UAS.cBa/Scer\GAL4^ey.PB

(Bui et al., 2000)

eya² has visible | recessive phenotype, suppressible by Scer\GAL4^ey.PB/Mmus\Eya3^UAS.cBa

(Bui et al., 2000)

NOT suppressed by

Statement

Reference

eya^E4/eya² has visible | adult stage phenotype, non-suppressible by Cap-H2^Z3-0019/Cap-H2[+]

(Tian et al., 2019)

eya^E4/eya² has abnormal size | adult stage phenotype, non-suppressible by Cap-H2^Z3-0019/Cap-H2[+]

(Tian et al., 2019)

eya² has visible | adult stage phenotype, non-suppressible by so^UAS.cUa/Scer\GAL4^ey.PU

(Weasner et al., 2016)

Suppressor of

Statement

Reference

eya² is a suppressor of viable phenotype of DNaseII^lo

(Liu et al., 2012)

Other

Statement

Reference

Abl², eya^A188/eya² has lethal - all die before end of larval stage phenotype

(Xiong et al., 2009)

Abl³/Abl², eya^A188/eya² has lethal - all die before end of larval stage phenotype

(Xiong et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

eya^cs/eya² has eye phenotype, enhanceable by Pc¹/Pc[+]

(Tian et al., 2019)

eya^WT.UAS/eya² has eye phenotype, enhanceable by Scer\GAL4^unspecified/Abl^K417N.UAS

(Xiong et al., 2009)

NOT Enhanced by

Statement

Reference

eya^E4/eya² has eye phenotype, non-enhanceable by Cap-H2^Z3-0019/Cap-H2[+]

(Tian et al., 2019)

Suppressed by

Statement

Reference

eya^E4/eya² has eye phenotype, suppressible | partially by Cap-H2^Z3-5163/Cap-H2^Z3-0019

(Tian et al., 2019)

eya^cs/eya² has eye phenotype, suppressible | partially by E(z)[+]/E(z)⁶⁰

(Tian et al., 2019)

eya² has eye phenotype, suppressible by so^{VP16.UAS.FL.NT}/Scer\GAL4^ey.PU

(Weasner et al., 2016)

eya² has eye phenotype, suppressible by Scer\GAL4^ey.PB/Mmus\Eya2^UAS.cBa

(Bui et al., 2000, Bonini et al., 1997)

eya² has eye phenotype, suppressible by Mmus\Eya1^UAS.cBa/Scer\GAL4^ey.PB

(Bui et al., 2000)

eya² has eye phenotype, suppressible by Scer\GAL4^ey.PB/Mmus\Eya3^UAS.cBa

(Bui et al., 2000)

NOT suppressed by

Statement

Reference

eya^E4/eya² has eye phenotype, non-suppressible by Cap-H2^Z3-0019/Cap-H2[+]

(Tian et al., 2019)

eya² has eye phenotype, non-suppressible by so^UAS.cUa/Scer\GAL4^ey.PU

(Weasner et al., 2016)

NOT Enhancer of

Statement

Reference

eya² is a non-enhancer of eye phenotype of pb^Rev3.HSPB

(Benassayag et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^dpp.blk1/eya² is a suppressor of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

NOT Suppressor of

Statement

Reference

eya² is a non-suppressor of adult subretinal glial cell | ectopic phenotype of gish¹

(Hummel et al., 2002)

eya² is a non-suppressor of lamina anlage glial cell | ectopic phenotype of gish¹

(Hummel et al., 2002)

eya² is a non-suppressor of lamina anlage | ectopic phenotype of gish¹

(Hummel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The eyeless phenotype of eya² adult mutants can be partially rescued by expression of so^{Scer\UAS.FL.NT.T:Hsim\VP16} (but not so^Scer\UAS.cUa) under the control of Scer\GAL4^ey.PU in the mutant background.

(Weasner et al., 2016)

Flies carrying homozygous eya² and DNaseII^lo mutations die at the pupal stage.

(Liu et al., 2012)

Coexpression of Abl^{K417N.Scer\UAS} reduces the efficiency of eya^WT.Scer\UAS to rescue the 'eyeless' phenotype of eya² from 100% to 40%.

(Xiong et al., 2009)

Flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 in a eya² background do not develop ectopic eyes, in contrast to flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 in a wild-type background.

(Bonini et al., 1997)

Xenogenetic Interactions

Statement

Reference

Mmus\Eya2^Scer\UAS.cBa restores eye formation in eya² homozygotes when expressed under the control of Scer\GAL4^ey.PB. The eyes produced have a pattern of ommatidial units with bristle cells similar to wild-type, and the pattern of photoreceptor neurons is normal.

(Bonini et al., 1997)

Complementation and Rescue Data

Rescued by

eya² is rescued by eya^RB.enh1+E+2

(Weasner et al., 2016)

eya² is rescued by eya^+tGR

(Jin et al., 2013)

eya² is rescued by eya^D493N.GR

(Jin et al., 2013)

eya² is rescued by eya^E728Q.GR

(Jin et al., 2013)

eya² is rescued by eya^NQ.GR

(Jin et al., 2013)

eya² is rescued by eya^+tGR

(Jusiak et al., 2012)

eya² is rescued by eya^SA.GR

(Jusiak et al., 2012)

eya² is rescued by eya^SDE.GR

(Jusiak et al., 2012)

eya² is rescued by eya^Hsp70Bb.PL

(Liu et al., 2012)

eya² is rescued by eya^Y4.Hsp70Bb

(Liu et al., 2012)

eya² is rescued by eya^WT.UAS/Scer\GAL4^dpp.blk1

(Xiong et al., 2009)

eya² is rescued by eya^{WT.UAS.NLS-MUT}/Scer\GAL4^dpp.blk1

(Xiong et al., 2009)

eya² is rescued by eya^UAS.Tag:FLAG/Scer\GAL4^dpp.blk1

(Mutsuddi et al., 2005)

eya² is rescued by Scer\GAL4^ey.PB/eya^UAS.cBa

(Bui et al., 2000)

eya² is rescued by Scer\GAL4^unspecified/eya^UAS.cBa

(Zimmerman et al., 2000)

eya² is rescued by Scer\GAL4^unspecified/eya^UAS.cBb

(Zimmerman et al., 2000)

eya² is rescued by eya^II.hs

(Leiserson et al., 1998)

eya² is rescued by eya^I.hs

(Leiserson et al., 1998)

Partially rescued by

eya² is partially rescued by eya^RB.enh1

(Weasner et al., 2016)

eya² is partially rescued by eya^RB.enhE

(Weasner et al., 2016)

eya² is partially rescued by eya^RB.enh1+E

(Weasner et al., 2016)

eya² is partially rescued by eya^RB.enh1+s+2

(Weasner et al., 2016)

eya² is partially rescued by eya^{WT.UAS.Tag:NLS(SV40-largeT)}/Scer\GAL4^dpp.blk1

(Xiong et al., 2009)

eya² is partially rescued by eya^{WT.UAS.Tag:NLS(SV40-largeT)}/Scer\GAL4^dpp.blk1/eya^{WT.UAS.Tag:Myr(v-Src)}

(Xiong et al., 2009)

eya² is partially rescued by eya^{WT.UAS.Tag:NLS(SV40-largeT)}/Scer\GAL4^dpp.blk1/eya^{K699Q.UAS.Tag:Myr(v-Src)}

(Xiong et al., 2009)

eya² is partially rescued by Scer\GAL4^dpp.blk1/eya^{T497M.UAS.Tag:FLAG}

(Mutsuddi et al., 2005)

eya² is partially rescued by eya^{E528K.UAS.Tag:FLAG}/Scer\GAL4^dpp.blk1

(Mutsuddi et al., 2005)

eya² is partially rescued by eya^{R715G.UAS.Tag:FLAG}/Scer\GAL4^dpp.blk1

(Mutsuddi et al., 2005)

eya² is partially rescued by Scer\GAL4^dpp.blk1/eya^{G594S.UAS.Tag:FLAG}

(Mutsuddi et al., 2005)

eya² is partially rescued by eya^{S655P.UAS.Tag:FLAG}/Scer\GAL4^dpp.blk1

(Mutsuddi et al., 2005)

eya² is partially rescued by Scer\GAL4^ey.PB/eya^UAS.cHa

(Rayapureddi et al., 2003)

eya² is partially rescued by eya^D493N.UAS/Scer\GAL4^ey.PB

(Rayapureddi et al., 2003)

eya² is partially rescued by eya^K699Q.UAS/Scer\GAL4^dpp.blk1

(Tootle et al., 2003)

eya² is partially rescued by eya^D493N.UAS/Scer\GAL4^dpp.blk1

(Tootle et al., 2003)

eya² is partially rescued by eya^S670A.UAS/Scer\GAL4^dpp.blk1

(Tootle et al., 2003)

eya² is partially rescued by Scer\GAL4^dpp.blk1/eya^{D493N.D724N.UAS}

(Tootle et al., 2003)

eya² is partially rescued by eya^{D493N.S670A.UAS}/Scer\GAL4^dpp.blk1

(Tootle et al., 2003)

eya² is partially rescued by Scer\GAL4^eya.PB/eya^UAS.cBa

(Bui et al., 2000)

eya² is partially rescued by Scer\GAL4^ey.PB/eya^E7.UAS

(Bui et al., 2000)

eya² is partially rescued by Scer\GAL4^ey.PB/eya^E11.UAS

(Bui et al., 2000)

eya² is partially rescued by Scer\GAL4^ey.PB/eya^UAS.cBa

(Bonini et al., 1997)

eya² is partially rescued by Scer\GAL4^T155/eya^UAS.cBa

(Bonini et al., 1997)

eya² is partially rescued by Scer\GAL4^T59/eya^UAS.cBa

(Bonini et al., 1997)

eya² is partially rescued by Scer\GAL4^dpp.blk1/eya^UAS.cBa

(Bonini et al., 1997)

eya² is partially rescued by Scer\GAL4^upd1-E132/eya^UAS.cPa

(Pignoni et al., 1997)

eya² is partially rescued by Scer\GAL4^dpp.blk1/eya^UAS.cPa

(Pignoni et al., 1997)

Not rescued by

eya² is not rescued by eya^RB.enh2

(Weasner et al., 2016)

eya² is not rescued by eya^RB.enh3

(Weasner et al., 2016)

eya² is not rescued by eya^RB.enh4

(Weasner et al., 2016)

eya² is not rescued by eya^RB.enh1+2

(Weasner et al., 2016)

eya² is not rescued by eya^{RB.enh1+5bp+2}

(Weasner et al., 2016)

eya² is not rescued by eya^RB.+535.+840

(Weasner et al., 2016)

eya² is not rescued by eya^{D493N.Hsp70Bb}

(Liu et al., 2012)

eya² is not rescued by eya^{T497M.Hsp70Bb}

(Liu et al., 2012)

eya² is not rescued by eya^335.Hsp70Bb

(Liu et al., 2012)

eya² is not rescued by Scer\GAL4^dpp.blk1/eya^{WT.UAS.Tag:Myr(v-Src)}

(Xiong et al., 2009)

eya² is not rescued by Scer\GAL4^dpp.blk1/eya^{L673R.UAS.Tag:FLAG}

(Mutsuddi et al., 2005)

eya² is not rescued by eya^{T643I.UAS.Tag:FLAG}/Scer\GAL4^dpp.blk1

(Mutsuddi et al., 2005)

eya² is not rescued by Scer\GAL4^dpp.blk1/eya^{G723E.UAS.Tag:FLAG}

(Mutsuddi et al., 2005)

Comments

The no-eye phenotype of adult eya² homozygous mutants can be fully rescued by combination with eya^RB.enh1+E+2 and partially restored by combination with any of the following: eya^RB.enh1, eya^RB.enhE, eya^RB.enh1+E or eya^RB.enh1+s+2 but cannot be rescued by any of these transgenes: eya^RB.enh2, eya^RB.enh3, eya^RB.enh4, eya^RB.enh1+2, eya^{RB.enh1+5bp+2} and eya^RB.+535.+840.

(Weasner et al., 2016)

One copy of either eya^+tGR, eya^D493N.GR, eya^E728Q.GR or eya^NQ.GR rescues rescues eye formation in eya² homozygotes. The external and internal morphology of the eyes is indistinguishable from wild type.

(Jin et al., 2013)

One copy of eya^+tGR fully rescues eye formation in eya² homozygotes.

One copy of either eya^SA.GR or eya^SDE.GR rescues eye formation in eya² homozygotes. The external morphology of the eyes are indistinguishable from wild type and cross sections show a normal number and arrangement of photoreceptors.

(Jusiak et al., 2012)

Inducible expression of eya^Y4.Hsp70Bb from egg to pupa under the control of both Scer\GAL4^hs.PB and Scer\GAL80^ts.αTub84B rescues the eyes absent phenotype of eya² mutants.

Inducible expression of eya^Hsp70Bb.PL from egg to pupa under the control of both Scer\GAL4^hs.PB and Scer\GAL80^ts.αTub84B rescues the eyes absent phenotype of eya² mutants.

(Liu et al., 2012)

The posterior and lateral regions of the eye are rescued by eya^Scer\UAS.cPa expressed under the control of Scer\GAL4^dpp.blk1. The development of the neuronal array is rescued in the most posterior region of the eye disc by eya^Scer\UAS.cPa expressed under the control of Scer\GAL4^E132.

(Pignoni et al., 1997)

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type