FB2024_03 , released June 25, 2024
Allele: Dmel\cths.PB
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General Information
Symbol
Dmel\cths.PB
Species
D. melanogaster
Name
heat shock construct of Blochlinger
FlyBase ID
FBal0030995
Feature type
allele
Associated gene
Dmel\ct
Associated Insertion(s)
Carried in Construct
P{hsCut}
Key Links
Transgenic product class
wild_type
(Blochlinger et al., 1991)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Blochlinger et al., 1991)
Mutagen
in vitro construct
(Blochlinger et al., 1991)
Progenitor genotype
Carried in construct
P{hsCut}
Cytology
Description

Expression of the entire ct coding region from nucleotide -268 to +6682 is driven by an Hsp70 promoter.

(Blochlinger et al., 1991)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
ct
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ectopic expression of cths.PB during oogenesis produces egg chambers containing 30 polyploid nurse cell nuclei and two diploid oocyte nuclei in over 75% of cases. The two oocytes are located at opposite poles of the egg chamber. These egg chambers are probably 'compound egg chambers' containing two adjacent germline cysts.

      (Jackson and Blochlinger, 1997)

      Heat stimulation of wandering larvae for 90mins and of middle third instar for more than 90mins at 37oC results in larval or pupal lethality. Ectopic ct expression in the eye disc causes maldifferentiation of photoreceptor precursors and excessive cell death. Compound eyes of transformants heat shocked at middle third instar exhibit irregular arrays of ommatidia along the dorsoventral axis. Interommatidial bristles are lost or arrangement disturbed.

      (Awasaki et al., 1994)

      ch organs are transformed to as es identity, scolopales are absent.

      (Blochlinger et al., 1991)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      cths.PB
      hsCut
      (Johnston et al., 1998)
      Name Synonyms
      heat shock construct of Blochlinger
      Secondary FlyBase IDs
        References (5)