FB2024_03 , released June 25, 2024
Allele: Dmel\robo13
Open Close
General Information
Symbol
Dmel\robo13
Species
D. melanogaster
Name
FlyBase ID
FBal0032590
Feature type
allele
Associated gene
Dmel\robo1
Associated Insertion(s)
Carried in Construct
Also Known As
roboZ14
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Kidd et al., 1998)
Mutagen
ethyl methanesulfonate
(Kidd et al., 1998, Seeger et al., 1993)
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In embryonic stage 16 robo3 mutants, the medial axonal fascicle misroutes across the midline, whereas the intermediate and lateral fascicles extend laterally, some longitudinal glia migrate over the midline whereas some retain their lateral positions (100% penetrance). In robo3 embryos the pCC/vMP2 fascicle is affected, collapsing over the midline (100% penetrance). In robo3 mutants at stage 12.1 only the dMP2/MP1 fascicle extends along its normal trajectory (with 27.7% penetrance) and curves medially slightly more than in wild-type only at the most posterior end. At stage 14, the vMP2/pCC fascicle is always collapsed over the midline in robo3, but the dMP2/MP1 fascicle can form normally.

      (Kinrade and Hidalgo, 2004)

      Fas2-expressing axons are seen crossing back and forth across the midline in homozygous embryos, in contrast to wild type. Heterozygous embryos show only extremely rare midline crossing defects.

      (Crowner et al., 2002)

      Some, but not all, interface glia migrate over the CNS midline. Longitudinal connectives are somewhat closer to the midline than in wild type, and the pCC/MP2 fascicle may cross the midline. Expression of roboScer\UAS.cKa driven by Scer\GAL4s.gcm rescues the lateral positions of the glia but does not rescue the axonal misrouting across the midline of robo3 mutants.

      (Kinrade et al., 2001)

      Fas2 positive neurons, including the pCC, cross the midline in robo3 embryos.

      (Kidd et al., 1998)

      "Fuzzy commisure" phenotype in embryonic central nervous system.

      (Seeger et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Statement
      Reference

      robo13 is an enhancer of abnormal neuroanatomy phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo[+]/robo13 is an enhancer of abnormal neuroanatomy phenotype of lolaORE120

      (Crowner et al., 2002)
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Statement
      Reference

      robo13 is an enhancer of larval MP1 neuron phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo13 is an enhancer of dMP2 neuron phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo13 is an enhancer of fascicle phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo13 is an enhancer of intermediate longitudinal fascicle phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo13 is an enhancer of medial longitudinal fascicle phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo13 is an enhancer of pCC neuron phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo13 is an enhancer of vMP2 neuron phenotype of gcmΔP1

      (Kinrade and Hidalgo, 2004)

      robo[+]/robo13 is an enhancer of larval longitudinal connective phenotype of lolaORE120

      (Crowner et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In gcmΔP1 robo3 double mutants, some embryonic central nervous system Fas2-positive fascicles collapse across the midline, with full collapse observed in 12.5% of cases and collapse from one side of the embryo (i.e. one hemisegment) in 22.6% of cases. This phenotype is never observed in either mutant alone. In gcmΔP1 robo3 double mutants, 31% of Fas2-positive axons also misroute towards the muscle, which is a phenotype observed with less frequency in gcm mutants. In gcmΔP1 robo3 double mutant stage 12.1 embryos both dMP2/MP1 and vMP2/pCC fascicles are affected, as the vMP2/pCC fascicle collapses completely over the midline (100% penetrance) and the dMP2/MP1 fascicle either does not extend or misroutes towards the muscle (89%). In gcmΔP1 robo3 double mutant stage 14 embryos there is a synergistic effect on both fascicles: the vMP2/pCC fascicle is completely collapsed over the midline (96% penetrance) and the dMP2/MP1 fascicle is missing (97% penetrance). The effect of glial loss is in severe gcmΔP1 robo3 double mutant embryos in which the longitudinal fascicles either collapse along the midline (63.3% penetrance when visualised with Avic\GFPScer\UAS.T:Hsap\Myr2) or misroute severely towards the periphery and exit the central nervous system (13.3% penetrance when visualised with Avic\GFPScer\UAS.T:Hsap\Myr2). However, when MP2 axons in these embryos misexpress leaScer\UAS.T:Hsap\MYC, under the control of Scer\GAL415J2, in the MP2 neurons, they do not extend longitudinally. Instead, they either do not grow (36.6% penetrance) and remain stunted at the base of the commissures, or they leave the central nervous system and misroute towards the muscle (30.7% penetrance). Ectopic expression of leaScer\UAS.T:Hsap\MYC under the control of Scer\GAL415J2 in a robo3 mutant embryo can displace Fas2-positive axons laterally and longitudinally.

      (Kinrade and Hidalgo, 2004)

      93% of lolaORE120 robo3/lolaORE120 embryos show Fas2-expressing axons crossing the midline. There are an average of 4.4 crossovers per affected embryo.

      (Crowner et al., 2002)

      Df(2L)ast2 robo3 double heterozygotes show little if any midline crossing defects in the embryonic central nervous system.

      (Wills et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      robo13 is rescued by robo1UAS.cKa/Scer\GAL4ftz.ng

      (Kidd et al., 1998)

      robo13 is rescued by Scer\GAL4elav.PLu/robo1UAS.cKa

      (Kidd et al., 1998)

      robo13 is rescued by robo1+t16

      (Kidd et al., 1998)
      Partially rescued by

      robo13 is partially rescued by Scer\GAL4s.gcm/robo1UAS.cKa

      (Kinrade et al., 2001)
      Comments

      The axon guidance phenotype is rescued in all segments by roboScer\UAS.cKa expressed under the control of Scer\GAL4elav.PLu. The pCC axon phenotype is also rescued by roboScer\UAS.cKa expressed under the control of Scer\GAL4ftz.ng.

      (Kidd et al., 1998)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Induced on: Fas3 null chromosome.

      (Kidd et al., 1998)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Z14
      (Kidd et al., 1998)
      robo13
      (Ing et al., 2007)
      robo3
      roboZ14
      (Kinrade and Hidalgo, 2004, Kinrade et al., 2001)
      roboz14
      (Crowner et al., 2002)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)