FB2024_03 , released June 25, 2024
Allele: Dhyd\MinosThs.PF
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General Information
Symbol
Dhyd\Minos\Ths.PF
Species
D. hydei
Name
heat shock construct of Franz
FlyBase ID
FBal0033602
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

An Hsp70 promoter drives expression of an intronless Dhyd\Minos\Ths.PF gene.

Left hand repeat of Dhyd\Minos is replaced by the Hsp70 promoter.

456bp XbaI-XmnI fragment of Hsp70 promoter and 206bp of untranslated leader sequence replace the 5' inverted repeat of Dhyd\Minos\T.

Hsp70 promoter drives expresson of Dhyd\Minos\T missing the 5' terminus.

Dhyd\Minos transposase gene is under the control of a heat shock promoter.

Allele components
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Carried in plasmid pHSS6hsMi2 and injected into D.melanogaster preblastoderm embryos as a source of transposase. Also carried in P{hsILMiT} and used to generate transgenic D.melanogaster flies containing a helper chromosome providing transposase.

Used as a Dhyd\Minos transposase source assayed in the D.melanogaster genome.

Carried in plasmid pHSS6hsMi2, expressed in D.melanogaster flies as a source of transposase.

A defective Dhyd\Minos transposon that is inserted into the D.melanogaster genome is stable in the absence of Dhyd\Minos\Ths.PF but is mobilised at relatively high frequencies in the presence of transposase.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Dhyd\Minos\Ths.PF
Name Synonyms
heat shock construct of Franz
Secondary FlyBase IDs
    References (9)