FB2024_03 , released June 25, 2024
Allele: Dmel\DeltaMtnA.PF
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General Information
Symbol
Dmel\DeltaMtnA.PF
Species
D. melanogaster
Name
Metallothionein A promoter construct of Fehon
FlyBase ID
FBal0033606
Feature type
allele
Associated gene
Dmel\Delta
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct
(Klueg et al., 1998, Frise et al., 1996, Fehon et al., 1990)
Progenitor genotype
Cytology
Description

Construct: Dl1 cDNA, which contains the entire coding sequence from the major embryonic Dl transcript (FBrf0048107), expression is driven by MtnA promoter.

(Fehon et al., 1990)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      S2 cells expressing DlMtnA.PF can form homotypic aggregates.

      (Parks et al., 2006)

      S2 cells that express DlC301S.MtnA or DlC301Y.MtnA are able to bind to and aggregate with N-expressing cells at significant, albeit reduced, frequencies compared to S2 cells that express DlMtnA.PF.

      (Parks et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      S2 cells expressing DlMtnA.PF can form heterotypic aggregates with S2 cells expressing NMGIIa.MtnA.

      (Parks et al., 2006)

      In S2 cells treated with p115dsRNA.cKa for 96hrs, the percentage of cells with at least one golgi stack is the same before and after DlMtnA.PF induction.

      (Kondylis and Rabouille, 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid, and transfected into S2 cells. The different Dl isoforms in these cells have been characterised. Also used in assays to determine whether trans-endocytosis of Dl by N expressing cells is dependent on the Dl intracellular domain.

      (Klueg et al., 1998)

      Carried in plasmid "DeltaWT", transfected into S2 cells and used in aggregation assays to compare the affinity of Dl and N proteins with that of Ser and N proteins.

      (Klueg and Muskavitch, 1999)

      Carried in a plasmid and transfected into S2 cells.

      (Fetchko et al., 2002, Rand et al., 2000, Fehon et al., 1990)

      Carried in a plasmid, transfected into S2 cells and EH34A3 cells (a shi1 cell line) and used to determine whether intercellular trafficking of N protein requires shi-dependent subcellular trafficking of Dl protein.

      (Parks et al., 2000)

      Expressed in S2 cells, purified and used as a substrate for glycosylation assays in vitro.

      (Panin et al., 2002)

      Carried in plasmid " pMT:DeltaWT " and transfected into S3 cells. Also transfected into S2 cells and used in cell-cell aggregation assays.

      (Klueg et al., 2002)

      Transfected into S2 cells.

      (Bland et al., 2003)

      DlMtnA.PF is used to monitor the flow of endosomes in the golgi and endoplasmic reticulum.

      (Kondylis and Rabouille, 2003)

      Carried in a plasmid and transfected S2 cells for use in cell aggregation assays.

      (Parks et al., 2006)

      Carried in plasmid "pMtDl" and expressed in Schneider S2 cells upon CuSO4 induction. Protein signalling indicates proper sorting amd membrane insertion of the protein.

      (Sun and Artavanis-Tsakonas, 1996)

      Carried in a plasmid, transfected into Schneider S2 cells, and used to study the ability of numb to inhibit Dl-dependent N activity.

      (Frise et al., 1996)

      Carried in a plasmid, transfected into Schneider S2 cells and the effects of exogenous Dl on Xenopus retinal eye development is assayed by filling the eye cup with S2 cells (S2 cells have access to the vitreal surface of the retina).

      (Ahmad, 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      DeltaMtnA.PF
      DeltaWT
      (Parks et al., 2000)
      DlMtnA.PF
      pmt-Delta
      (Fetchko et al., 2002)
      Name Synonyms
      Metallothionein A promoter construct of Fehon
      (Fehon et al., 1990)
      Secondary FlyBase IDs
        References (18)