FlyBase Allele Report: Dmel\ttk[1e11]

General Information

Symbol

Dmel\ttk^1e11

Species

D. melanogaster

Name

FlyBase ID

FBal0035855

Feature type

allele

Associated gene

Dmel\ttk

Associated Insertion(s)

Carried in Construct

Also Known As

ttk^lell, ttk^le11

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

loss of function allele

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Giesen et al., 1997, Pritchard and Schubiger, 1996, Xiong and Montell, 1993)

hypomorphic allele - genetic evidence

(Vervoort et al., 1997)

loss of function allele

(Lai and Li, 1999)

Mutagen

jumpstarter activity

(Xiong and Montell, 1993)

P-element activity

(Giesen et al., 1997)

Progenitor genotype

ttk¹

(Xiong and Montell, 1993)

Cytology

Description

Deletion of 1.7kb of 3' P-element and 0.8kb of flanking genomic DNA that encodes the zinc fingers in the ttk p69 protein.

(Xiong and Montell, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:31,729,632..31,732,395 [+]

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. See Figure 4 of FBrf0155483. Mutation is a deletion of 1.7kb of 3' P-element and 0.8kb of flanking genomic DNA that encodes the zinc fingers in the ttk p69 protein according to FBrf0058514.

(French et al., 2003, Xiong and Montell, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell | cell autonomous | somatic clone

(Wang et al., 2006)

cone cell | P-stage | somatic clone

(Lai and Li, 1999)

cone cell | somatic clone | third instar larval stage

(Siddall et al., 2009)

dorsal appendage | maternal effect (with ttk¹)

(French et al., 2003)

dorsal appendage | maternal effect (with ttk^twp)

(French et al., 2003)

dorsal appendage | somatic clone

(Boyle and Berg, 2009)

egg | maternal effect (with ttk^twp)

(French et al., 2003)

egg chamber | maternal effect (with ttk^twp)

(French et al., 2003)

embryonic/larval glial cell | increased number

(Badenhorst, 2001)

embryonic/larval tritocerebral commissure | embryonic stage 15

(Page, 2004)

eye | somatic clone

(Siddall et al., 2009)

eye photoreceptor cell | somatic clone

(Lai and Li, 1999)

filzkorper

(Xiong and Montell, 1993)

follicle cell | maternal effect (with ttk^twp)

(French et al., 2003)

follicle cell | somatic clone

(Jordan et al., 2006)

interommatidial bristle | somatic clone

(Siddall et al., 2009)

larval abdominal 1 lch5 neuron

(Kennerdell and Carthew, 1998)

larval abdominal 2 lch5 neuron

(Kennerdell and Carthew, 1998)

larval abdominal 3 lch5 neuron

(Kennerdell and Carthew, 1998)

larval abdominal 4 lch5 neuron

(Kennerdell and Carthew, 1998)

larval abdominal 5 lch5 neuron

(Kennerdell and Carthew, 1998)

larval abdominal 6 lch5 neuron

(Kennerdell and Carthew, 1998)

larval abdominal 7 lch5 neuron

(Kennerdell and Carthew, 1998)

lens | somatic clone

(Siddall et al., 2009, Lai and Li, 1999)

mouth hook

(Xiong and Montell, 1993)

ommatidial precursor cluster | somatic clone

(Lai and Li, 1999)

ommatidium | somatic clone

(Siddall et al., 2009, Xiong and Montell, 1993)

oocyte | oogenesis stage S10B | somatic clone

(Boyle and Berg, 2009)

oocyte | oogenesis stage S13 | somatic clone

(Boyle and Berg, 2009)

photoreceptor cell R7

(Yamamoto et al., 1996)

photoreceptor cell R7 | somatic clone | increased number | third instar larval stage

(Siddall et al., 2009)

presumptive embryonic/larval tracheal system

(Araujo et al., 2007)

rhabdomere | somatic clone

(Lai and Li, 1999)

thecogen cell

(Vervoort et al., 1997)

Detailed Description

Statement

Reference

Heterozygous ttk^1e11 females lay normal eggs.

(Ge et al., 2015)

The nuclei in homozygous follicle cell clones are more densely packed as compared to the surrounding wild-type tissue.

(Technau et al., 2012)

Cells derived from mosaic clones of ttk^1e11 do not participate in dorsal appendage morphogenesis. Mutant cells clump together, block surrounding cells, and occasionally split the dorsal appendage.

Cells derived from mosaic clones of ttk^1e11 at S10B show dramatic apical constriction of normally cuboidal cells. Basal surfaces of mutant cells are slightly shrunken and rounder than control cells, but far larger than their apical surfaces. Mutant nuclei are more closely packed at later stages of oogenesis, but this effect is not pronounced at early stages.

Cells derived from mosaic clones of ttk^1e11 are taller than control cells.

(Boyle and Berg, 2009)

ttk^1e11 mutant eye clones display severe degeneration of the corneal lens, with ommatidia and sensory bristles failing to properly develop.

In 87% of ttk^1e11 mutant third instar eye disc clones, 2-3 presumptive R7 photoreceptor cells are observed seven rows posterior to the morphogenetic furrow, compared to just one R7 cell in control tissue. A reduced number of cone cells are observed in mutant clones.

(Siddall et al., 2009)

ttk^1e11 mutant embryos display defects in tracheal patterning.

(Araujo et al., 2007)

Follicle cells in ttk^1e11 clones show a decrease in the size of cell nuclei and quantification of the number of cells in these clones compared to the number in their sister clones shows that on average they are twice as large, which implies that ttk^1e11 mutant cells have undergone an extra round of mitotic division.

(Jordan et al., 2006)

In mosaic egg chambers in which border cells retain ttk[+] function, border cell migration is completed normally. However, homozygous border cells show a severe migration defect and remain at the anterior tip of the stage 10 egg chamber. Homozygous centripetal cells appear to be delayed in their inward migration.

(Wang et al., 2006)

ttk^1e11 mutant stage 15 embryos show defects in the separation of tritocerebral and mandibular commissures.

(Page, 2004)

Only 40% of eggs laid by ttk¹/ttk^1e11 mothers are wild-type,, the rest exhibit a dorsal appendage phenotype, 3% having only the nub of an appendage.

(French et al., 2003)

The second mitotic wave is normal in ttk^1e11 clones in the eye disc.

(Baonza et al., 2002)

Glia undergo ectopic rounds of replication in mutant embryos. Stage 12 embryos have 17.8 +/- 2.23 glia associated with the longitudinal connectives (compared to 9.9 +/- 0.76 in wild-type embryos).

(Badenhorst, 2001)

Homozygous clones in the adult eye cause degeneration of the corneal lens and a failure of photoreceptor development. Rhabdomeres of photoreceptors are not observed in clones, but residual cellular structures in the mutant ommatidia are still recognizable. Genetically mosaic ommatidia are not seen near the boundary of the clone. Ectopic neurons are not seen between or below the developing ommatidia in homozygous clones in third instar larvae. Larval eye development seems close to normal in the absence of ttk, although the ommatidial clusters in clones were somewhat disorganised. Cone cell development appears normal. In homozygous clones in the developing pupal eye, some cone cells failed to develop. Photoreceptors appear normal even up to midpupal stage.

(Lai and Li, 1999)

The number of lch5 neurons is approximately doubled in homozygous embryos.

(Kennerdell and Carthew, 1998)

Neurons and sheath cells of es and ch organs are duplicated at the expense of support cells. There is no modification of md neurons, they are present in the normal number and pattern.

(Vervoort et al., 1997)

ttk^bose1/ttk^1e11 flies have extra R7 photoreceptor cells in some ommatidia.

(Yamamoto et al., 1996)

Embryonic development arrests before dorsal closure. Transformation of ommatidial cells into nonommatidial cell types in mosaic flies.

(Xiong and Montell, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ttk^1e11 has visible | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

Enhancer of

Statement

Reference

ttk[+]/ttk^1e11 is an enhancer of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

ttk[+]/ttk^1e11 is an enhancer of visible phenotype of phyl²/phyl⁴

(Pi et al., 2001)

Suppressor of

Statement

Reference

ttk[+]/ttk^1e11 is a suppressor of visible phenotype of phyl²/phyl⁴

(Pi et al., 2001)

Other

Statement

Reference

mir-318^Δ1/mir-318^Δ2, ttk[+]/ttk^1e11 has lethal - all die during embryonic stage phenotype

(Ge et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

ttk^1e11 has interommatidial bristle | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

ttk^1e11 has photoreceptor cell R7 | increased number | somatic clone | third instar larval stage phenotype, suppressible by lz^77a7

(Siddall et al., 2009)

ttk^1e11 has eye | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

ttk^1e11 has ommatidium | somatic clone phenotype, suppressible by lz^mr2

(Siddall et al., 2009)

Enhancer of

Statement

Reference

ttk[+]/ttk^1e11 is an enhancer of egg phenotype of mir-318^Δ1/mir-318^Δ2

(Ge et al., 2015)

ttk[+]/ttk^1e11 is an enhancer of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

ttk[+]/ttk^1e11 is an enhancer of adult abdominal segment 3 & chaeta phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is an enhancer of adult abdominal segment 3 & tormogen cell | supernumerary phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is an enhancer of adult abdominal segment 3 & trichogen cell | supernumerary phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is an enhancer of adult abdominal segment 4 & chaeta phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is an enhancer of adult abdominal segment 4 & tormogen cell | supernumerary phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is an enhancer of adult abdominal segment 4 & trichogen cell | supernumerary phenotype of phyl²/phyl⁴

(Pi et al., 2001)

NOT Enhancer of

Statement

Reference

ttk[+]/ttk^1e11 is a non-enhancer of wing margin bristle | ectopic phenotype of hry⁴¹

(Badenhorst et al., 2002)

Suppressor of

Statement

Reference

ttk[+]/ttk^1e11 is a suppressor of adult abdominal segment 3 & microchaeta phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is a suppressor of adult abdominal segment 4 & microchaeta phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is a suppressor of mesothoracic tergum & chaeta phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is a suppressor of mesothoracic tergum & microchaeta phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is a suppressor of mesothoracic tergum & tormogen cell | supernumerary phenotype of phyl²/phyl⁴

(Pi et al., 2001)

ttk[+]/ttk^1e11 is a suppressor of mesothoracic tergum & trichogen cell | supernumerary phenotype of phyl²/phyl⁴

(Pi et al., 2001)

NOT Suppressor of

Statement

Reference

ttk[+]/ttk^1e11 is a non-suppressor of wing margin bristle | ectopic phenotype of hry⁴¹

(Badenhorst et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of ttk^1e11 enhances the abnormal egg phenotype seen in mir-318^Δ1/mir-318^Δ2 mutants, including short dorsal appendages. None of the mir-318^Δ1 mir-318^Δ2/ttk^1e11 mutant flies are able to hatch into first instar larval females. A more severe reduction in chorion gene amplification is seen.

(Ge et al., 2015)

The eye phenotype observed in ttk^1e11 clones is partially suppressed in a lz^mr2 mutant background. Ommatidial structures and sensory bristles are present, and the eye appears less scarred.

No recruitment of R7 precursor cells is observed when ttk^1e11 mutant cells are generated in a lz^77a7 background.

(Siddall et al., 2009)

The addition of ttk^1e11/+ to h⁴¹/+ animals does not lead to a significant ectopic wing margin bristle phenotype.

(Badenhorst et al., 2002)

The partial lethality due to run^Scer\UAS.cLa; Scer\GAL4^nos.PG (3% viable) is partially suppressed by maternal heterozygosity for ttk^1e11 (rescues to 21% viable).

(Wheeler et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

ttk¹

(Lai and Li, 1999)

Partially complements

ttk¹

(French et al., 2003)

Fails to complement

ttk^twp

(French et al., 2003)

ttk¹⁵⁰

(Horn et al., 2003)

Partially rescued by

ttk^twp/ttk^1e11 is partially rescued by ttk^p69.UAS/Scer\GAL4^hs.PB

(French et al., 2003)

Not rescued by

ttk^twp/ttk^1e11 is not rescued by ttk^p88.UAS/Scer\GAL4^hs.PB

(French et al., 2003)

Comments

94% of the ommatidia in ttk¹/ttk^1e11 flies were wild-type, as opposed to 50-60% in ttk¹ homozygotes.

(Lai and Li, 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

41754

w^*; P{neoFRT}82B ttk^1e11/TM6B, Tb⁺

Kyoto

107607

w^*; ttk^1e11/TM3, ry^* Sb¹

Notes on Origin

Discoverer

Comments

Disrupts the mRNA encoding ttk protein p69 and p88.

(Xiong and Montell, 1993)

Excision allele.

(Giesen et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Ttk^1e11

(Wheeler et al., 2002)

ttk^1E11

(Technau et al., 2012)

ttk^1e11

(Liaw, 2022, Kitatani et al., 2020, Knapp et al., 2019, Simon et al., 2019, Davis and Rebay, 2017, Liaw, 2016, Romani et al., 2016, Ge et al., 2015, Huang et al., 2013, Hwang and Rulifson, 2011, Walrad et al., 2011, Boyle and Berg, 2009, Siddall et al., 2009, Christensen et al., 2008.4.15, Araujo et al., 2007, Letizia et al., 2007, Jordan et al., 2006, Wang et al., 2006, Page, 2004)

ttk^ie11

(Araujo et al., 2007)

ttk^le11

(Chen et al., 2009, Vander Zwan et al., 2003, Merabet et al., 2002)

ttk^lell

(Liaw, 2022, French et al., 2003, Pi et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (47)

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