FlyBase Allele Report: Dmel\tws[j11C8]

General Information

Symbol

Dmel\tws^j11C8

Species

D. melanogaster

Name

FlyBase ID

FBal0035892

Feature type

allele

Associated gene

Dmel\tws

Associated Insertion(s)

P{lacW}tws^j11C8

Carried in Construct

Also Known As

tws^P, twins^P

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}tws^j11C8

Cytology

Description

P{lacW} has inserted into a tip of the second intron, 4bp downstream from an exon-intron boundary.

(Shiomi et al., 1994)

P{lacW} insertion into one of the exon/intron junctions 7bp downstream of the first initiation codon.

(Uemura et al., 1993)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:10,133,211..10,133,218 [-]

(Bajpai et al., 2004, Spradling et al., 1999, BDGP Project Members, 1994-1999, Uemura et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval brain | third instar larval stage (with Df(3R)by62)

(Merigliano et al., 2017)

embryonic/larval brain | third instar larval stage (with tws¹⁹⁶)

(Merigliano et al., 2017)

embryonic/larval brain | third instar larval stage (with tws⁴³⁰)

(Merigliano et al., 2017)

embryonic/larval optic lobe | somatic clone

(Chabu and Doe, 2009)

larval neuroblast | somatic clone | increased number

(Chabu and Doe, 2009)

larval neuroblast | somatic clone | increased number (with Df(3R)Exel6265)

(Chabu and Doe, 2009)

macrochaeta

(Mayer-Jaekel et al., 1994)

mitochondrion

(Wang et al., 2011)

nuclear chromosome | third instar larval stage (with Df(3R)by62)

(Merigliano et al., 2017)

nuclear chromosome | third instar larval stage (with tws¹⁹⁶)

(Merigliano et al., 2017)

nuclear chromosome | third instar larval stage (with tws⁴³⁰)

(Merigliano et al., 2017)

nucleus | third instar larval stage (with Df(3R)by62)

(Merigliano et al., 2017)

nucleus | third instar larval stage (with tws¹⁹⁶)

(Merigliano et al., 2017)

wing disc | larval stage

(Bajpai et al., 2004, Mayer-Jaekel et al., 1994)

wing margin | somatic clone

(Bajpai et al., 2004)

Detailed Description

Statement

Reference

tws^j11C8/Df(3R)by62 (exhibit the strongest phenotype), tws^j11C8/tws¹⁹⁶ and tws^j11C8/tws⁴³⁰ third instar larval brains exhibit high frequencies of cells with chromosome aberrations (CABs, metaphases displaying from one to five CABs and metaphases with more than five CABs often showing an extensive chromosome fragmentation) compared to controls. Specifically they exhibit: chromatid and isochromatid deletions and exchanges of chromatid and chromosome type. Most aberrations and exchanges are complete, namely they contain all the elements that give rise to the CAB.

Under normal conditions tws^j11C8/Df(3R)by62 and tws^j11C8/tws¹⁹⁶ third instar larval brains exhibit an increase in the average frequency of nuclei with γ-H2Av foci (indicative of double strand breaks) compared to controls. After 5 Gy X-ray treatment, in mutant and control brains, the frequency of nuclei with γ-H2Av-positive foci peaks at 5 minutes post irradiation (PIR) and remains high at 30 minutes PIR to progressively decrease at 1 and 2 hr PIR. At 4 and 6 hr PIR, the frequencies of nuclei with foci remain high in mutant brains while they drop in controls.

(Merigliano et al., 2017)

Large tubular mitochondria are seen in photoreceptor neurons of mutant flies. The intercristal network in these mitochondria appears swollen and disorganised.

(Wang et al., 2011)

Larval single cell tws^j11C8 neuroblast clones often result in multiple neuroblasts compared to controls.

Mutant third instar optic lobes contain only neuroblasts and no recognisable neuroectoderm. Neuroblasts expand up to four-cell layers deep into the optic lobe and have no columnar cell morphology. There is no detectable phenotype in the inner optic lobe.

tws^j11C8/Df(3R)Exel6265 trans-heterozygote mutant larval brains have more neuroblasts per brain lobe compared to wild-type. The neuroblasts in trans-heterozygotes are smaller than normal.

(Chabu and Doe, 2009)

tws^j11C8 homozygous animals die during early pupal stages. During the third larval instar, the wing discs of these animals exhibit mirror-symmetric pattern duplications. tws^j11C8/tws^j11C8 somatic clones in the wing lead to notching of the wing margin - the frequency of the clones observed in imaginal discs is far higher than the number of clones observed in pharate adult/adult flies, suggesting many that clones die during pupal stages. The presence of more tws⁺ twinspots than clones in imaginal discs, suggests that clones also die during larval stages.

(Bajpai et al., 2004)

Homozygotes die as early pupae. Third instar larvae have duplicated wing imaginal discs. tws^j11C8/tws^aar-1 flies die as pharate adults or young adults which have duplicated bristles. tws^j11C8/Df(3R)by62 flies die as early pupae and third instar larvae have small wing imaginal discs.

(Mayer-Jaekel et al., 1994)

Wing discs exhibit unusual outgrowths in the posterior compartment, this overgrown region resembles the wing pouch. Similar outgrowths are seen on the haltere disc. Eye-antennal and leg defects exhibit no defects. Homozygotes exhibit extended larval periods and wandering third instar larvae are larger than wild-type.

(Uemura et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tws^j11C8 has increased cell number | somatic clone phenotype, suppressible by aPKC^k06403/aPKC[+]

(Chabu and Doe, 2009)

Enhancer of

Statement

Reference

tws[+]/tws^j11C8 is an enhancer | maternal effect of female semi-sterile | dominant phenotype of gwl^scant, polo[+]/polo¹

(Rangone et al., 2011)

Suppressor of

Statement

Reference

tws[+]/tws^j11C8 is a suppressor | partially of abnormal meiotic cell cycle | oogenesis phenotype of gwl¹⁸⁰/gwl^Sr18

(Kim et al., 2012)

NOT Suppressor of

Statement

Reference

tws[+]/tws^j11C8 is a non-suppressor of abnormal meiotic cell cycle | oogenesis phenotype of endos⁰⁰⁰⁰³/endos^215-4

(Kim et al., 2012)

Other

Statement

Reference

gwl^scant, polo[+]/polo¹¹, tws[+]/tws^j11C8 has female sterile | dominant phenotype

(Rangone et al., 2011)

gwl^scant, polo[+]/polo¹, tws[+]/tws^j11C8 has female sterile | dominant phenotype

(Rangone et al., 2011)

polo[+]/polo¹, tws^j11C8 has female fertile phenotype

(Rangone et al., 2011)

polo[+]/polo¹¹, tws^j11C8 has female semi-sterile | dominant phenotype

(Rangone et al., 2011)

gwl[+]/gwl^scant, tws^j11C8 has female semi-sterile | dominant phenotype

(Rangone et al., 2011)

polo¹¹, tws[+]/tws^j11C8 has lethal | dominant | maternal effect | embryonic stage phenotype

(Wang et al., 2011)

gwl^scant, tws[+]/tws^j11C8 has lethal | dominant | maternal effect | embryonic stage phenotype

(Wang et al., 2011)

Scer\GAL4^{VP16.mat.αTub67C}, gwl^L.UASp, tws^j11C8 has abnormal meiotic cell cycle | dominant phenotype

(Wang et al., 2011)

Scer\GAL4^{VP16.mat.αTub67C}, gwl^L.UASp, tws^j11C8 has sterile | dominant phenotype

(Wang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(3R)Exel6265/tws^j11C8 has embryonic/larval optic lobe phenotype, suppressible | partially by aPKC^k06403/aPKC[+]

(Chabu and Doe, 2009)

Df(3R)Exel6265/tws^j11C8 has larval neuroblast | increased number phenotype, suppressible | partially by aPKC^k06403/aPKC[+]

(Chabu and Doe, 2009)

tws^j11C8 has larval neuroblast | increased number | somatic clone phenotype, suppressible by aPKC^k06403/aPKC[+]

(Chabu and Doe, 2009)

tws^j11C8 has wing margin phenotype, suppressible by arm^{S10.UAS.Tag:MYC}/Scer\GAL4^vg.PM

(Bajpai et al., 2004)

NOT suppressed by

Statement

Reference

tws^j11C8 has wing margin phenotype, non-suppressible by Scer\GAL4^vg.PM/arm^{S2.UAS.Tag:MYC}

(Bajpai et al., 2004)

Enhancer of

Statement

Reference

tws[+]/tws^j11C8 is an enhancer of centrosome | embryonic stage 4 | maternal effect phenotype of polo¹¹

(Wang et al., 2011)

tws[+]/tws^j11C8 is an enhancer of mesothoracic tergum | ectopic phenotype of Scer\GAL4^vg.PM, sgg^UAS.cSa

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is an enhancer of wing phenotype of Scer\GAL4^vg.PM, sgg^UAS.cSa

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is an enhancer of wing margin phenotype of Scer\GAL4^vg.PM, sgg^UAS.cSa

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is an enhancer of wing phenotype of Scer\GAL4^vg.PM, pan^ΔN.UAS

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is an enhancer of wing margin phenotype of Scer\GAL4^vg.PM, pan^ΔN.UAS

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is an enhancer of wing margin phenotype of Scer\GAL4^vg.PM, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Bajpai et al., 2004)

NOT Enhancer of

Statement

Reference

tws[+]/tws^j11C8 is a non-enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^vg.PM, arm^{S10.UAS.Tag:MYC}

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is a non-enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^vg.PM, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}

(Bajpai et al., 2004)

Suppressor of

Statement

Reference

tws[+]/tws^j11C8 is a suppressor | partially of wing margin bristle | ectopic phenotype of Scer\GAL4^vg.PM, dsh^UAS.cNa

(Bajpai et al., 2004)

NOT Suppressor of

Statement

Reference

tws[+]/tws^j11C8 is a non-suppressor of wing margin bristle | ectopic phenotype of Scer\GAL4^vg.PM, arm^{S10.UAS.Tag:MYC}

(Bajpai et al., 2004)

tws[+]/tws^j11C8 is a non-suppressor of wing margin bristle | ectopic phenotype of Scer\GAL4^vg.PM, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}

(Bajpai et al., 2004)

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, gwl^L.UASp, tws^j11C8 has spindle | embryonic stage phenotype

(Wang et al., 2011)

Scer\GAL4^vg.PM, pan^ΔN.UAS, tws[+]/tws^j11C8 has mesothoracic tergum | ectopic phenotype

(Bajpai et al., 2004)

Hsap\APC^UAS.cBa, Scer\GAL4^vg.PM, tws^j11C8 has wing margin phenotype

(Bajpai et al., 2004)

Hsap\APC^UAS.cBa, Scer\GAL4^vg.PM, tws[+]/tws^j11C8 has wing margin phenotype

(Bajpai et al., 2004)

Scer\GAL4^vg.PM, pan^ΔN.UAS, tws^j11C8 has mesothoracic tergum | ectopic phenotype

(Bajpai et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

tws^j11C8/+ suppresses many of the gwl⁷¹⁶/gwl¹⁰⁸⁰ and gwl⁷¹⁶/gwl²⁷⁹⁰ mutant phenotypes, with adults eclosing at the normal time with normal wing and eye morphologies. Males are fertile, but females lay eggs that do not develop.

tws^j11C8/+ suppresses many of the gwl⁷¹⁶/gwl¹⁸⁰ mutant phenotypes, with adults eclosing at the normal time with normal wing and eye morphologies. Males are fertile, but females lay eggs that do not develop. The mitotic index of mutant larval neuroblasts is near normal.

tws^j11C8/+ suppresses the endos^215-4 homozygous phenotype, with the animals developing into viable adults whose larval brains have wild-type mitotic parameters and normal chromosome condensation. Adult males are fertile, but females lay eggs that do not develop.

tws^j11C8/+ significantly rescues the meiotic maturation defect of gwl^Sr18/gwl¹⁸⁰ oocytes.

tws^j11C8/+ does not rescue the meiotic maturation defect of endos⁰⁰⁰⁰³/endos^215-4 oocytes.

(Kim et al., 2012)

tws^j11C8/+ enhances the reduced fertility phenotype of polo¹/+ gwl^scant/+ double heterozygous females, such that the triple heterozygotes are completely sterile.

(Rangone et al., 2011)

The embryos resulting from the cross between polo¹¹/+, tws^j11C8 mothers and wild-type fathers fail to hatch.

The embryos resulting from the cross between gwl^scant/+, tws^j11C8/+ mothers and wild-type fathers fail to hatch. These embryos abort very early during syncitial divisions with most or all centrosomes detached already in the first few cycles.

Overexpression of gwl^L.Scer\UAS in eggs/embryos under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} from tws^j11C8/+ mothers is almost completely lethal. Most eggs/embryos are blocked in metaphase of meiosis I, even after 4 to 6 hours post-laying. Only a few eggs manage to complete meiosis and attempt to initiate embryonic development. However, these embryos usually abort in the first mitosis, displaying several aberrant structures with condensed chromatin at the center of small spindles. The few mitotic nuclei observed show a very high incidence of detached centrosomes.

Overexpression of gwl^{K87R.Scer\UAS.P\T.T:Hsap\MYC} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} from tws^j11C8/+ mothers has no effect on embryonic viability.

(Wang et al., 2011)

Larval tws^j11C8 mutant clones in an aPKC^k06403/+ background contain an intermediate number of neuroblasts per clone, partially rescuing the single cell tws^j11C8 clone phenotype.

The third instar optic lobe of tws^j11C8/Df(3R)Exel6265 in a aPKC^k06403/+ background have a partially reduced number of optic lobe neuroblasts, partially rescuing the tws^j11C8/Df(3R)Exel6265 phenotype.

(Chabu and Doe, 2009)

The serrated posterior wing margin phenotype of tws^Scer\UAS.cBa; Scer\GAL4^vg.PM flies is suppressed by arm^{S10.Scer\UAS.T:Hsap\MYC}, but not by arm^{S2.Scer\UAS.T:Hsap\MYC}.

(Bajpai et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

tws⁰²⁴¹⁴

(BDGP Project Members, 1994-1999)

tws^s1801

(BDGP Project Members, 1994-1999)

Partially rescued by

tws^j11C8 is partially rescued by tws^UAS.cBa/Scer\GAL4^en-e16E

(Bajpai et al., 2004)

Not rescued by

tws^j11C8 is not rescued by tws^UAS.cBa/Scer\GAL4^hs.PB

(Bajpai et al., 2004)

Comments

tws^Scer\UAS.cBa; Scer\GAL4^hs.PB fails to rescue the late larval/early pupal lethal phenotype of tws^j11C8 homozygotes.

(Bajpai et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

L. and Y. Jan.

Uemura.

(Uemura et al., 1993)

Comments

Reversion tests suggest that the insertion is responsible for pupal lethality and the wing disc phenotype.

(Uemura et al., 1993)

Complements: l(3)j9A5^j9A5.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

l(3)11C8

(Uemura et al., 1993)

l(3)j11C8

(Spradling et al., 1999)

l(3)j11C8^j11C8

twins^P

(Kim et al., 2012, Mayer-Jaekel et al., 1994)

tws^J11C8

(Wang et al., 2011)

tws^P

(Larouche et al., 2021, Garrido et al., 2020, Merigliano et al., 2017, Rangone et al., 2011, Wang et al., 2011, Chabu and Doe, 2009, Cai et al., 2008, Bajpai et al., 2004, Mayer-Jaekel and Hemmings, 1995, Mayer-Jaekel and Hemmings, 1994, Uemura et al., 1993)

tws^j11C8

tws^p

(Wang et al., 2011)

Name Synonyms

twinsP

(Chabu and Doe, 2009)

Secondary FlyBase IDs

FBal0010884

References (18)

Report Sections

Open Close