FlyBase Allele Report: Dmel\H[E31]

General Information

Symbol

Dmel\H^E31

Species

D. melanogaster

Name

FlyBase ID

FBal0038852

Feature type

allele

Associated gene

Dmel\H

Associated Insertion(s)

P{3'lacW}H^E31

Carried in Construct

Also Known As

H³¹

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bang et al., 1995)

Mutagen

P-element activity

(Schweisguth and Posakony, 1994)

Delta2-3 transposase

(Schweisguth and Lecourtois, 1998)

Progenitor genotype

H^D179

(Schweisguth and Lecourtois, 1998, Schweisguth and Posakony, 1994)

Associated Insertion(s)

P{3'lacW}H^E31

Cytology

Description

Imprecise excision of the P{lacW} element, removing the 5' P-element end, the Ecol\lacZ gene and part of the w^+mC sequence. The deletion extends from position -2046 in w^+mC to position +3101 of H, deleting sequences encoding the first 804 amino acids of the H protein.

(Schweisguth and Lecourtois, 1998)

Deletion of about two thirds of the protein coding region.

(Schweisguth and Posakony, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior orbital bristle

(Barolo et al., 2002)

chaeta | somatic clone

(Duan et al., 2011)

intestinal stem cell | somatic clone

(Bardin et al., 2010)

macrochaeta

(Coumailleau and Schweisguth, 2014, Duan et al., 2011, Barolo et al., 2002, Schweisguth, 1999, Bang et al., 1995)

macrochaeta | adult stage

(Dai et al., 2013)

macrochaeta | somatic clone

(Schweisguth and Lecourtois, 1998)

mechanosensory sensory organ | adult stage

(Dai et al., 2013)

medial row of triple row of wing sensilla

(Schweisguth and Lecourtois, 1998)

mesothoracic tergum & macrochaeta | somatic clone

(Schweisguth and Lecourtois, 1998)

microchaeta

(Schweisguth, 1999)

ocellar bristle

(Barolo et al., 2002)

sense organ | adult stage

(Lai et al., 1998)

tormogen cell | ectopic

(Duan et al., 2011)

tormogen cell | embryonic stage

(Schweisguth and Lecourtois, 1998)

trichogen cell

(Duan et al., 2011)

wing vein

(Lai and Rubin, 2001, Schweisguth and Lecourtois, 1998)

wing vein | cell autonomous | somatic clone

(Schweisguth and Lecourtois, 1998)

wing vein L5

(Lai and Rubin, 2001, Thumm and Kadowaki, 2001)

Detailed Description

Statement

Reference

Heterozygous H^E31 mutant flies exhibit a mild double-socket macrochaetae phenotype and a weak bristle loss phenotype.

(Coumailleau and Schweisguth, 2014)

H^E31/+ mutants exhibit socket-to-shaft transformations in mechanosensory organs, particularly evident amongst head macrochaetes.

(Dai et al., 2013)

Heterozygotes show a double-socketing phenotype in a few macrochaetae on the notum.

Homozygous clones in the adult notum lack external sensory organ structures.

(Duan et al., 2011)

Whereas wild-type intestinal stem cell (ISC) clones proliferate over time, homozygous ISC clones fail to grow, forming either very small groups of cells or remaining as single cells at 14 days after clone induction. Cell fate appears to be affected in the mutant clones; many of the mutant cells appear to have lost ISC characteristics without properly differentiating into enteroblasts when marker expression is analysed at 6 days after clone induction.

(Bardin et al., 2010)

Heterozygotes show developmental defects in the macrochaetae (shaft-to-socket conversions or bristle loss).

(Barolo et al., 2002)

Mutants show a small amount of wing vein truncation mostly at the tip of wing vein L5.

(Lai and Rubin, 2001)

Heterozygotes have a shortened wing vein L5.

(Thumm and Kadowaki, 2001)

Heterozygotes show a few double socket bristles on the notum.

(Schweisguth, 1999)

Heterozygotes have several missing or double socket sensory organs, particularly on the head.

(Lai et al., 1998)

Homozygous clones in the notum are characterised by a loss of bristle phenotype. The ratio between wild-type bristles and wild-type epidermal cells along the clone border is 0.1 (compared to 0.05 for control clones) suggesting that H^E31 cells produce a weaker inhibitory signal than wild-type cells. No veins form in the wings of homozygous pharate adults. Double sockets are seen at the position of the stout bristles on the anterior wing margin. Homozygous clones interrupt vein formation in a cell autonomous manner. The ventral nerve cord appears similar to wild-type in stage 14 homozygous embryos. A single socket cell per external sense organ is seen in most positions in homozygous embryos. Occasionally, missing or extra socket cells are seen.

(Schweisguth and Lecourtois, 1998)

The bristle loss phenotype of H²⁰/H^E31 can be suppressed by deleting components of the E(spl)-complex. The degree of suppression depends on both the number and identity of E(spl)-complex transcription units removed. Rescued bristles display a double socket phenotype. Clonal analysis revealed that the gro mutant bristle tufting phenotype is epistatic to the H null bristle loss phenotype.

(Bang et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

H^E31 has visible | dominant phenotype, enhanceable by insv^23B

(Coumailleau and Schweisguth, 2014)

H^E31, insv^23B has visible | dominant phenotype, enhanceable by insb^Δ1/Tes^+t22/qkr54B^+t22/CG14478^+t22

(Coumailleau and Schweisguth, 2014)

H^E31 has visible | dominant phenotype, enhanceable by Tes^+t22/qkr54B^+t22/CG14478^+t22

(Coumailleau and Schweisguth, 2014)

H^E31 has visible | dominant phenotype, enhanceable by insv^unspecified

(Duan et al., 2011)

H^E31 has visible | dominant phenotype, enhanceable by gro^E73/gro[+]

(Barolo et al., 2002)

H^E31 has visible | dominant phenotype, enhanceable by CtBP[+]/CtBP^87De-10

(Barolo et al., 2002)

H^E31 has visible phenotype, enhanceable by Scer\GAL4^VMQ/neur^αRING.UAS

(Lai and Rubin, 2001)

H^E31 has visible phenotype, enhanceable by neur^UAS.cLa/Scer\GAL4^VMQ

(Lai and Rubin, 2001)

H^E31 has visible | dominant phenotype, enhanceable by E(spl)m8-HLH^K:CAACdel

(Lai et al., 1998)

H^E31 has visible | dominant phenotype, enhanceable by E(spl)m8-HLH^K1K2mut

(Lai et al., 1998)

NOT Enhanced by

Statement

Reference

H^E31 has visible | dominant phenotype, non-enhanceable by E(spl)m8-HLH^tLa

(Lai et al., 1998)

Suppressed by

Statement

Reference

H^E31 has visible | dominant phenotype, suppressible by Scer\GAL4^sca.PU/insv^UAS.cDa

(Dai et al., 2013)

H^E31 has visible | dominant phenotype, suppressible by Scer\GAL4^sca.PU/Mmus\Bend6^UAS.Tag:MYC

(Dai et al., 2013)

H^E31 has visible | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/insv^UAS.cDa

(Duan et al., 2011)

H^E31 has visible | dominant phenotype, suppressible by CG6194[+]/Atg4b^P0997

(Thumm and Kadowaki, 2001)

Suppressor of

Statement

Reference

H[+]/H^E31 is a suppressor of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008)

H[+]/H^E31 is a suppressor of visible | heat sensitive phenotype of N^l1N-ts1

(Barolo et al., 2002)

H[+]/H^E31 is a suppressor of visible phenotype of rg^γ6

(Shamloula et al., 2002)

Other

Statement

Reference

H^E31, Su(H)^del47 has abnormal size | somatic clone phenotype

(Bardin et al., 2010)

H^E31, Ras85D^e1F, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

H^E31 has macrochaeta phenotype, enhanceable by insb^Δ1/Tes^+t22/qkr54B^+t22/CG14478^+t22

(Coumailleau and Schweisguth, 2014)

H^E31 has macrochaeta phenotype, enhanceable by insv^23B

(Coumailleau and Schweisguth, 2014)

H^E31, insv^23B has macrochaeta phenotype, enhanceable by insb^Δ1/Tes^+t22/qkr54B^+t22/CG14478^+t22

(Coumailleau and Schweisguth, 2014)

H^E31 has macrochaeta phenotype, enhanceable by insv^unspecified

(Duan et al., 2011)

H^E31 has tormogen cell | ectopic phenotype, enhanceable by insv^unspecified

(Duan et al., 2011)

H^E31 has trichogen cell phenotype, enhanceable by insv^unspecified

(Duan et al., 2011)

H^E31 has macrochaeta phenotype, enhanceable by gro^E73/gro[+]

(Barolo et al., 2002)

H^E31 has ocellar bristle phenotype, enhanceable by gro^E73/gro[+]

(Barolo et al., 2002)

H^E31 has anterior orbital bristle phenotype, enhanceable by CtBP[+]/CtBP^87De-10

(Barolo et al., 2002)

H^E31 has macrochaeta phenotype, enhanceable by CtBP[+]/CtBP^87De-10

(Barolo et al., 2002)

H^E31 has wing vein phenotype, enhanceable by neur^UAS.cLa/Scer\GAL4^VMQ

(Lai and Rubin, 2001)

H^E31 has wing vein L5 phenotype, enhanceable by Scer\GAL4^VMQ/neur^αRING.UAS

(Lai and Rubin, 2001)

H^E31 has wing vein phenotype, enhanceable by Scer\GAL4^VMQ/neur^αRING.UAS

(Lai and Rubin, 2001)

H^E31 has wing vein L5 phenotype, enhanceable by neur^UAS.cLa/Scer\GAL4^VMQ

(Lai and Rubin, 2001)

H^E31 has macrochaeta phenotype, enhanceable by Pros26[+]/Prosβ6¹

(Schweisguth, 1999)

H^E31 has microchaeta phenotype, enhanceable by Pros26[+]/Prosβ6¹

(Schweisguth, 1999)

H^E31 has macrochaeta phenotype, enhanceable by Prosβ2¹/Prosbeta2[+]

(Schweisguth, 1999)

H^E31 has microchaeta phenotype, enhanceable by Prosβ2¹/Prosbeta2[+]

(Schweisguth, 1999)

H^E31 has sense organ | adult stage phenotype, enhanceable by E(spl)m8-HLH^K:CAACdel

(Lai et al., 1998)

H^E31 has sense organ | adult stage phenotype, enhanceable by E(spl)m8-HLH^K1K2mut

(Lai et al., 1998)

NOT Enhanced by

Statement

Reference

H^E31 has sense organ | adult stage phenotype, non-enhanceable by E(spl)m8-HLH^tLa

(Lai et al., 1998)

Suppressed by

Statement

Reference

H^E31 has mechanosensory sensory organ | adult stage phenotype, suppressible by Scer\GAL4^sca.PU/Mmus\Bend6^UAS.Tag:MYC

(Dai et al., 2013)

H^E31 has macrochaeta | adult stage phenotype, suppressible by Scer\GAL4^sca.PU/Mmus\Bend6^UAS.Tag:MYC

(Dai et al., 2013)

H^E31 has mechanosensory sensory organ | adult stage phenotype, suppressible by Scer\GAL4^sca.PU/insv^UAS.cDa

(Dai et al., 2013)

H^E31 has macrochaeta | adult stage phenotype, suppressible by Scer\GAL4^sca.PU/insv^UAS.cDa

(Dai et al., 2013)

H^E31 has chaeta | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/insv^UAS.cDa

(Duan et al., 2011)

H^E31 has wing vein L5 phenotype, suppressible by CG6194[+]/Atg4b^P0997

(Thumm and Kadowaki, 2001)

Enhancer of

Statement

Reference

H[+]/H^E31 is an enhancer of macrochaeta phenotype of Prosβ2¹

(Schweisguth, 1999)

H[+]/H^E31 is an enhancer of microchaeta phenotype of Prosβ2¹

(Schweisguth, 1999)

H[+]/H^E31 is an enhancer of macrochaeta phenotype of Prosβ6¹

(Schweisguth, 1999)

H[+]/H^E31 is an enhancer of microchaeta phenotype of Prosβ6¹

(Schweisguth, 1999)

Suppressor of

Statement

Reference

H[+]/H^E31 is a suppressor of wing margin phenotype of Bx¹

(Bejarano et al., 2008)

H^E31 is a suppressor of wing disc phenotype of Psn^I2

(Koelzer and Klein, 2006)

H[+]/H^E31 is a suppressor of head phenotype of eyg^M3-12

(Chao et al., 2004)

H[+]/H^E31 is a suppressor | partially of eye phenotype of eyg¹/eyg^M3-12

(Chao et al., 2004)

H[+]/H^E31 is a suppressor of chaeta | heat sensitive phenotype of N^l1N-ts1

(Barolo et al., 2002)

H[+]/H^E31 is a suppressor of eye phenotype of rg^γ6

(Shamloula et al., 2002)

H^E31 is a suppressor of wing disc phenotype of ap^UGO35

(Klein et al., 2000)

H^E31 is a suppressor of wing disc phenotype of Psn^I2/Psn^B3

(Klein et al., 2000)

H^E31 is a suppressor of wing margin phenotype of Psn^I2/Psn^B3

(Klein et al., 2000)

H[+]/H^E31 is a suppressor of wing margin phenotype of sno^71e3

(Majumdar et al., 1997)

NOT Suppressor of

Statement

Reference

H^E31/H^E31 is a non-suppressor of intestinal stem cell | increased number | somatic clone phenotype of Su(H)^del47

(Bardin et al., 2010)

H^E31/H^E31 is a non-suppressor of wing disc phenotype of ap^rK568/ap^UGO35

(Koelzer and Klein, 2006)

H^E31 is a non-suppressor of wing disc phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

Other

Statement

Reference

H^E31, ap^UGO35 has wing margin phenotype

(Klein et al., 2000)

H^E31, Ras85D^e1F, pb^hs.PB has wing vein L5 phenotype

(Boube et al., 1998)

H^E31, Ras85D^e1F, pb^hs.PB has wing vein L4 phenotype

(Boube et al., 1998)

H^E31, Ras85D^e1F, pb^hs.PB has campaniform sensillum of dorsal radius phenotype

(Boube et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The bristle loss phenotype seen in H^E31/+ mutant flies is enhanced by loss of insb (using insb^Δ1, in which the function of CG14478, Tes and qkr54B has been restored using P{CG14478-Tes-qkr54B-mCherry} (which comprises of the CG14478^{T:Disc\RFP-mCherry}, Tes^{T:Disc\RFP-mCherry} and qkr54B^{T:Disc\RFP-mCherry} alleles). Expression of insb^T:Avic\GFP rescues this phenotype, returning the number of transformed macrochaetae to levels seen in H^E31 heterozygotes alone.

insv^23B enhances the double-socket macrochaetae phenotype seen in H^E31/+ mutants. The bristle loss phenotype is further enhanced by loss of insb, using insb^Δ1, in which the function of CG14478, Tes and qkr54B has been restored using P{CG14478-Tes-qkr54B-mCherry} (which comprises of the CG14478^{T:Disc\RFP-mCherry}, Tes^{T:Disc\RFP-mCherry} and qkr54B^{T:Disc\RFP-mCherry} alleles).

(Coumailleau and Schweisguth, 2014)

Expression of insv^Scer\UAS.cDa under the control of Scer\GAL4^sca.PU partially suppresses the socket-to-shaft transformations in mechanosensory organs observed in H^E31/+ mutants.

(Dai et al., 2013)

insv^unspecified ; H^E31/+ flies show double socketing of nearly all external sensory organs on the notum.

Homozygous H^E31 clones in the adult notum which are also expressing insv^Scer\UAS.cDa under the control of Scer\GAL4^tub.PU can develop external sensory organ structures.

(Duan et al., 2011)

H^E31 Su(H)^del47 double mutant intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells, as occurs in Su(H)^del47 single mutant clones.

H^E31 Df(3R)E(spl)δ-6 double mutant intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells, as occurs in Su(H)^del47 single mutant clones.

(Bardin et al., 2010)

In contrast to Psn^I2 single mutants, the dorsal/ventral boundary forms correctly in H^E31; Psn^I2 double mutants.

(Koelzer and Klein, 2006)

CtBP^87De-10 dominantly enhances the bristle defects seen in H^E31/+ flies. This enhancement is almost entirely due to shaft-socket transformations, with only a mild effect on bristle loss. gro^E73 dominantly enhances the bristle defects seen in H^E31/+ flies. Both the shaft-to-socket and bristle loss phenotypes are enhanced.

(Barolo et al., 2002)

The shortening of wing vein L5 seen in H^E31/+ flies is suppressed by CG6194^P0997/+.

(Thumm and Kadowaki, 2001)

ap^UGO35,H^E31 double mutants have large wing pouches with no margin structures.

(Klein et al., 2000)

Double heterozygotes of H^E31 with Pros26¹ or Prosβ2¹ show strong synergistic enhancement of the double socket bristle and microchaete phenotypes.

(Schweisguth, 1999)

pb^hs.PB H^E31/Ras85D^e1F triple heterozygotes have deletions of the distal portions of wing veins L4 and L5, up to and including the posterior crossvein. The spacing and position of the campaniform sensilla along the proximo-distal axis of wing vein L3 is altered.

(Boube et al., 1998)

The heterozygous phenotype is enhanced by a single copy of E(spl)^K1K2mut or E(spl)^K:CAACdel but not by E(spl)^tLa.

(Lai et al., 1998)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Bend6^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sca.PU partially suppresses the socket-to-shaft transformations in mechanosensory organs observed in H^E31/+ mutants.

(Dai et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Germ line clonal analysis indicates that H activity is not essential for embryogenesis.

(Schweisguth and Lecourtois, 1998)

Double mutant analysis fails to establish a strict epistatic relationship between H and Su(H) for the specification of sensory organ precursor fate.

(Schweisguth and Posakony, 1994)

H²⁰/H^E31 is used as the standard null genotype.

(Bang et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

H³¹

(Thumm and Kadowaki, 2001, Furriols and Bray, 2000)

H^E31

(Kavaler et al., 2018, Berndt et al., 2017, Dai et al., 2015, Coumailleau and Schweisguth, 2014, Duan et al., 2011, Walrad et al., 2011, Bardin et al., 2010, Stern et al., 2009, Bejarano et al., 2008, Koelzer and Klein, 2006)

Hairless^E31

(Dai et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (30)

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