FB2024_03 , released June 25, 2024
Allele: Dmel\hhUAS.cCa
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General Information
Symbol
Dmel\hhUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Capdevila
FlyBase ID
FBal0039403
Feature type
allele
Associated gene
Dmel\hh
Associated Insertion(s)
Carried in Construct
P{UAS-hh.C}
Also Known As
UAS-hh
Key Links
Transgenic product class
wild_type
(Capdevila and Guerrero, 1994)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Capdevila and Guerrero, 1994)
Mutagen
in vitro construct
(Biehs et al., 1998, Bilder and Scott, 1998, Capdevila and Guerrero, 1994)
Progenitor genotype
Carried in construct
P{UAS-hh.C}
Cytology
Description

UASt regulatory sequences drive expression of a 3091bp HindIII/EcoRI fragment carrying the entire hh open reading frame.

(Capdevila and Guerrero, 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
hh
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      1st posterior cell, with Scer\GAL4en-e16E

      (Vervoort et al., 1999)

      anterior-posterior compartment boundary of the wing blade, with Scer\GAL4C-734

      (Mullor et al., 1997)

      anterior-posterior compartment boundary of the wing blade, with Scer\GAL4dpp.blk1

      (Mullor et al., 1997)

      campaniform sensillum of dorsal radius, with Scer\GAL4en-e16E

      (Vervoort et al., 1999)

      dorsal row of double row of wing sensilla | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      dorsal row of double row of wing sensilla | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      dorsal row of triple row of wing sensilla | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      dorsal row of triple row of wing sensilla | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      eye, with Scer\GAL4ey.PH

      (Cavodeassi et al., 1999)

      female genital disc, with Scer\GAL4cad-em459

      (Gorfinkiel et al., 2003)

      female genitalia | increased number, with Scer\GAL4cad-em459

      (Gorfinkiel et al., 2003)

      macrochaeta | ectopic, with Scer\GAL4C-734

      (Mullor et al., 1997)

      male genital disc, with Scer\GAL4cad-em459

      (Gorfinkiel et al., 2003)

      male genitalia | increased number, with Scer\GAL4cad-em459

      (Gorfinkiel et al., 2003)

      medial row of triple row of wing sensilla | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      medial row of triple row of wing sensilla | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      prescutum, with Scer\GAL4pnr-MD237

      (Mullor et al., 1997)

      scutellum, with Scer\GAL4C-734

      (Mullor et al., 1997)

      scutellum, with Scer\GAL4en-e16E

      (Mullor et al., 1997)

      scutellum, with Scer\GAL4pnr-MD237

      (Mullor et al., 1997)

      scutellum & macrochaeta, with Scer\GAL4C-734

      (Mullor et al., 1997)

      scutellum & macrochaeta, with Scer\GAL4en-e16E

      (Mullor et al., 1997)

      scutum, with Scer\GAL4pnr-MD237

      (Mullor et al., 1997)

      sensory mother cell, with Scer\GAL4C-734

      (Mullor et al., 1997)

      sensory mother cell, with Scer\GAL4dpp.blk1

      (Mullor et al., 1997)

      sensory mother cell, with Scer\GAL4en-e16E

      (Mullor et al., 1997)

      ventral row of double row of wing sensilla | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      ventral row of double row of wing sensilla | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      ventral row of triple row of wing sensilla | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      ventral row of triple row of wing sensilla | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      wing, with Scer\GAL4en-e16E

      (Biehs et al., 1998)

      wing | anterior, with Scer\GAL430A

      (Guillen et al., 1995)

      wing | anterior, with Scer\GAL4Bx-MS1096

      (Guillen et al., 1995)

      wing | anterior, with Scer\GAL4C-765

      (Guillen et al., 1995)

      wing | pharate adult stage, with Scer\GAL4MS941

      (Capdevila and Guerrero, 1994)

      wing blade anterior compartment | pharate adult stage, with Scer\GAL4Bx-MS1096

      (Capdevila and Guerrero, 1994)

      wing disc, with Scer\GAL4ap-md544

      (Nfonsam et al., 2012)

      wing vein, with Scer\GAL471B

      (Mullor et al., 1997)

      wing vein L1 | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      wing vein L1 | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      wing vein L2 | pharate adult stage, with Scer\GAL430A

      (Capdevila and Guerrero, 1994)

      wing vein L2 | pharate adult stage, with Scer\GAL471B

      (Capdevila and Guerrero, 1994)

      wing vein L2 | pharate adult stage, with Scer\GAL4zfh2-MS209

      (Capdevila and Guerrero, 1994)

      wing vein L3, with Scer\GAL4en-e16E

      (Vervoort et al., 1999, Mullor et al., 1997)

      wing vein L3, with Scer\GAL4MD-638

      (Mullor et al., 1997)

      wing vein L3 | pharate adult stage, with Scer\GAL471B

      (Capdevila and Guerrero, 1994)

      wing vein L3 | pharate adult stage, with Scer\GAL4MS941

      (Capdevila and Guerrero, 1994)
      Detailed Description
      Statement
      Reference

      Expression of hhScer\UAS.cCa under the control of Scer\GAL4ap-md544 results in an increase in size of the wing disc.

      (Nfonsam et al., 2012)

      Expression of hhScer\UAS.cCa under the control of Scer\GAL4cad-em459 results in duplication of the genital discs and the external genital structures in both males and females.

      (Gorfinkiel et al., 2003)

      hhScer\UAS.cCa when expressed under the control of Scer\GAL4en-e16E produces embryos that have no significant defects in their hindgut.

      (Sanchez Soriano and Russell, 2000)

      Expression of hhScer\UAS.cCa under the control of Scer\GAL4ey.PH dorsalises the eye, severely reducing its size.

      (Cavodeassi et al., 1999)

      When driven by Scer\GAL4en-e16E the width of the L3-L4 intervein area is increased. Wing vein L3 is displaced anteriorly, truncated and partly fused to L2. L3 specific sensilla are displaced together in most but not all cases.

      (Vervoort et al., 1999)

      Veins L3 and L4 are further apart in the wings of flies expressing hhScer\UAS.cCa under the control of Scer\GAL4en-e16E than in wild-type flies.

      (Biehs et al., 1998)

      Produces a repetitive distribution of all wing sensory organ precursors normally located close to the A/P compartment border, when expressed using either Scer\GAL4C-734 or Scer\GAL4dpp.blk1. Produces overgrowth and ectopic bristles in the scutellum when expressed using Scer\GAL4C-734 at 25oC. Almost completely represses scutum and prescutum formation, and produces an enlarged scutellum, when expressed using Scer\GAL4pnr-MD237. Produces an anterior displacement of wing vein 3 and the L3 sensillae when expressed using Scer\GAL4en-e16E at 17oC. The wing vein is displaced more anteriorly, the L3 sensilla are absent, the costa is duplicated and the scutellum is covered with extra bristles when hhScer\UAS.cCa is expressed using Scer\GAL4en-e16E at 25oC.

      (Mullor et al., 1997)

      When expression is driven by Scer\GAL4Bx-MS1096, posterior transformation of the anterior wing margin occurs. Less extreme anterior to posterior transformations are caused by Scer\GAL430A and Scer\GAL4C-765.

      (Guillen et al., 1995)

      Ectopic expression of dpp in the wing of pharate adults, in the Scer\GAL4 expressing line MS209 and 30A line, induces overgrowth with a mirror image duplications restricted to the anterior edge of the wing. Ectopic expression in 71A Scer\GAL4 expressing line causes branching of L2 and L3. Ectopic expression in MS941 Scer\GAL4 expressing line causes defects in the anterior wing margin and vein L3. Ectopic expression in MS1096 Scer\GAL4 expressing line causes severe disorganisation and necrosis in the anterior wing only.

      (Capdevila and Guerrero, 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Scer\GAL4en-e16E, hhUAS.cCa has visible phenotype, suppressible by kncol-1

      (Vervoort et al., 1999)
      Enhancer of
      Statement
      Reference

      Scer\GAL4GMR.PF/hhUAS.cCa is an enhancer of visible phenotype of roDOM

      (Chanut et al., 2000)
      Suppressor of
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4en-e16E, hhUAS.cCa has wing vein L3 phenotype, suppressible by kncol-1

      (Vervoort et al., 1999)

      Scer\GAL4en-e16E, hhUAS.cCa has submarginal cell phenotype, suppressible by kncol-1

      (Vervoort et al., 1999)
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of hhScer\UAS.cCa suppresses the eye overgrowth phenotype seen when DlScer\UAS.cDa and mir-7Scer\UAS.cLb are expressed under the control of Scer\GAL4ey.PH. The phenotype is seen with 100% penetrance.

      (Da Ros et al., 2013)

      Co-expression of hhScer\UAS.cCa results in enlarged wing discs containing a larger field (30-40% of disc surface area) of differentiating ectopic eye precursors compared to expression of eyScer\UAS.cHa alone under the control of Scer\GAL4ap-md544.

      (Nfonsam et al., 2012)

      When dppScer\UAS.cSa, eyScer\UAS.cHa and hhScer\UAS.cCa are driven by Scer\GAL430A, photoreceptor differentiation is induced in both compartments of the wing disc. When hhScer\UAS.cCa, eyaScer\UAS.cPa and eyScer\UAS.cHa are driven by Scer\GAL430A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment.

      (Pappu et al., 2003)

      When expression is driven by Scer\GAL4GMR.PF in a roDOM mutant, the enhancement of the stop-furrow phenotype is strong.

      (Chanut et al., 2000)

      The wing phenotype caused by hhScer\UAS.cCa expressed under the control of Scer\GAL4en-e16E is suppressed by kncol-1, restoring an almost wild type position of wing vein L3 and its associated sensillae. An ectopic crossvein can be seen in the L2-L3 intervein.

      (Vervoort et al., 1999)

      ptcScer\UAS.cJa Scer\GAL471B flies carrying a relatively weakly expressing hhScer\UAS.cCa insertion line have wing discs that are indistinguishable from wild-type. ptcScer\UAS.cJa Scer\GAL471B flies carrying a more strongly expressing hhScer\UAS.cCa insertion line lack all veins in the anterior compartment, while vein 4 is present. Flies expressing both ptcScer\UAS.cJa and hhScer\UAS.cCa using Scer\GAL4MD-638 show recovery of the posterior compartment veins, ectopic induction of vein 3 and L3 sensilla and an enlargement of the region between veins 3 and 4.

      (Mullor et al., 1997)

      The phenotype produced by ectopic expression of hhScer\UAS.cCa using Scer\GAL4C-335a is partially rescued by fuJ. The phenotype produced by ectopic expression of hhScer\UAS.cCa using Scer\GAL430A is partially rescued by fu41.

      (Sanchez-Herrero et al., 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      hhScer\UAS.cCa
      hhUAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Capdevila
      (Capdevila and Guerrero, 1994)
      Secondary FlyBase IDs
        References (21)