Construct: Double site compensatory mutation, 3A to G and 9C to G, to allow base pairing with the mutations of wDR18.
Eye colour phenotype of wDR18 flies cannot be suppressed by one copy of P{U1a-3G,9G}.
Carried in plasmid "U1a-3G,9G" and expressed in Schneider tissue culture cells. Splicing efficiency of wDR18 is suppressed greater than 4-5 fold.
Carried in plasmid "U1a-3G,9G" and expressed in Schneider tissue culture cells. Splicing efficiency of wDR18 is suppressed greater than 4-5 fold.