Construct: Single site compensatory mutation, 3A to G, within snRNA:U1:21D to allow base pairing with the +6U to C mutation of wDR18.
lethal | recessive
Suppression of the eye colour phenotype of wDR18 by one copy of P{U1a-3G}.
Carried in plasmid "U1b-3G" and expressed in Schneider tissue culture cells. Splicing efficiency of wDR18 is suppressed 4-5 fold.
Carried in plasmid "U1b-3G" and expressed in Schneider tissue culture cells. Splicing efficiency of wDR18 is suppressed 4-5 fold.