Construct: Single site compensatory mutation, 9C to G, within snRNA:U1:21D to allow base pairing with the -1G to C mutation of wDR18.
Eye colour phenotype of wDR18 flies cannot be suppressed by one copy of P{U1b-9G}.
Carried in plasmid "U1b-9G" and expressed in Schneider tissue culture cells. Splicing efficiency of wDR18 is suppressed 2 fold.
Carried in plasmid "U1b-9G" and expressed in Schneider tissue culture cells. Splicing efficiency of wDR18 is suppressed 2 fold.