In third instar larva, expressed throughout the presumptive leg, with higher levels of expression in rings; in pupal legs, these rings seen to locate proximal to constrictions; in adult legs, corresponding higher levels of expression proximal to the joints.
In second instar larva, expressed throughout the wing disc, at slightly higher level in the ventral rgion; this pattern evolves into a 2-3-cell-wide stripe that saddles the D/V interface.