FB2024_03 , released June 25, 2024
Allele: Ecol\lacZCecA1.A11
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General Information
Symbol
Ecol\lacZCecA1.A11
Species
E. coli
Name
FlyBase ID
FBal0043844
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{CecA1.A11-lacZ}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Roos et al., 1998, Petersen et al., 1995, Roos et al., 1994, Engstrom et al., 1993)
Progenitor genotype
Ecol\lacZCecA1.A10
(Engstrom et al., 1993)
Carried in construct
P{CecA1.A11-lacZ}
Cytology
Description

lacZ expression is regulated by upstream untranslated region of CecA1 from -68 to +62bp. CecA1 sequence includes R2 motif but not the R1 and κΒ-like motifs.

(Petersen et al., 1995)

Deletion of a SphI-SalI fragment from position -760 to -68.

(Engstrom et al., 1993)
Allele components
Component
Use(s)
Regulatory region(s)
CecA1_pA11
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference

      Fails to show LPS-inducible expression in blood cell line assay (see report for EcollacZCecA1.A10RA).

      (Engstrom et al., 1993)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Cannot be transactivated by Dif gene product.

      (Petersen et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pA11" and transfected into mbn-2 cells to identify cis regulatory sequences necessary for CecA1 expression.

      (Roos et al., 1998)

      Carried in plasmid "pA11", transfected into mbn-2 cells and assayed for expression.

      (Miele et al., 2001)

      Carried in plasmid "pA11" and expressed in mbn-2 (malignant blood neoplasm) cells to identify minimal cis regulatory sequences necessary for the induction of CecA1 expression.

      (Engstrom et al., 1993)

      Carried in plasmid "pA11". Assayed in mbn-2 cells.

      (Petersen et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZCecA1.A11
      lacZCecA1.A11
      Name Synonyms
      Secondary FlyBase IDs
        References (6)