lacZ expression is regulated by upstream untranslated region of CecA1 from -760bp to -105bp and -69bp to +62bp. CecA1 sequence includes R2 motif but not the R1 and κΒ-like motifs.
Carried in plasmid "pA10", transiently expressed in mbn-2 cells to assay the requirements of GATA site for CecA1 mediated expression. Also cotransfected into mbn-2 cells to assay the requirements of Dif for maximal transactivation of CecA1.
Carried in plasmid "pA15" and transfected into mbn-2 cells to identify cis regulatory sequences necessary for CecA1 expression.
Carried in plasmid "pA15". Assayed in mbn-2 cells.
Carried in plasmid "pA10", transiently expressed in mbn-2 cells to assay the requirements of GATA site for CecA1 mediated expression. Also cotransfected into mbn-2 cells to assay the requirements of Dif for maximal transactivation of CecA1.