In the presence of a copy of P{hsp70:Mos1}, the rate of germline excision of M{peach} from P{M{peach}w^pch} is 16.9% (tested in D.melanogaster). Five out of seven insertions of P{hsp70:peach} result in a statistically significant reduction in the rate of germline excision of M{peach} from P{M{peach}w^pch} caused by P{hsp70:Mos1} (tested in D.melanogaster).

Overproduction of transposase causes a smaller frequency of excision in the Dmau\w^pch assay, regulation by overproduction inhibition (OPI).

In the presence of P{hsp70:Mos1} the uniform peach colour of w::Dmau\w^pch.tGa changes to dark reddish-brown, in the absence of heat shock, resulting in a mosaic red phenotype. Homozygous P{hsp70:Mos1} genotypes have a lower frequency of w::Dmau\w^pch.tGa germline excision compared to heterozygous P{hsp70:Mos1}/+ genotypes: negative complementation. Double heterozygotes carrying two different insertions of P{hsp70:Mos1} still exhibit a lower frequency of w::Dmau\w^pch.tGa germline excision confirming negative complementation is not caused by transvection. The effect of heat shock on males and females carrying P{hsp70:Mos1} is a significant decrease in the frequency of excision. In the absence of heat shock P{G292R} and P{E297K} exhibit negative complementation when heterozygous with P{hsp70:Mos1} with respect to the extent of Dmau\mariner element excision from w::Dmau\w^pch.tGa in the soma and P{G292R}, P{T42I} and P{E297K} exhibit negative complementation when heterozygous with P{hsp70:Mos1} in the germline.