FlyBase Allele Report: Dmel\dpp[UAS.cNa]

General Information

Symbol

Dmel\dpp^UAS.cNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Nellen

FlyBase ID

FBal0049439

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

P{UAS(-FRT)dpp.N}

Also Known As

UAS-dpp, UAS dpp

Key Links

Transgenic product class

wild_type

(Nellen et al., 1996)

Mutagen

FLPase

Nature of the Allele

Transgenic product class

wild_type

(Nellen et al., 1996)

Mutagen

FLPase

(Tanimoto et al., 2000, Greenwood and Struhl, 1999, Lecuit and Cohen, 1997, Nellen et al., 1996)

Progenitor genotype

dpp^FRT.UAS

(Tanimoto et al., 2000, Greenwood and Struhl, 1999, Lecuit and Cohen, 1997, Burke and Basler, 1996, Nellen et al., 1996)

Carried in construct

P{UAS(-FRT)dpp.N}

Cytology

Description

FRT cassette has been excised, juxtaposing UAS regulatory sequences with dpp coding sequences.

(Nellen et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dpp

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PH

(Lee et al., 2007)

antenna | increased number, with Scer\GAL4^ey.PH

(Lee et al., 2007)

antennal disc, with Scer\GAL4^ey.PH

(Lee et al., 2007)

eye, with Scer\GAL4^ey.PH

(Lee et al., 2007)

eye disc, with Scer\GAL4^ey.PH

(Lee et al., 2007)

female germline stem cell, with Scer\GAL4^C587

(Xi et al., 2005)

female germline stem cell, with Scer\GAL4^dpp.PS

(Zhao et al., 2008)

female germline stem cell | ectopic, with Scer\GAL4^C587

(Kai and Spradling, 2004, Song et al., 2004)

female germline stem cell | ectopic | heat sensitive, with Scer\GAL4^hs.PB

(Song et al., 2004)

female germline stem cell | increased number, with Scer\GAL4^C587

(Kai et al., 2005)

germarium, with Scer\GAL4^hs.PB

(Xie and Spradling, 1998)

germarium cap cell, with Scer\GAL4^C587

(Kai et al., 2005)

germline cell | female | increased number, with Scer\GAL4^C587

(Zhu and Xie, 2003)

hub cell | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Kawase et al., 2004)

male germline stem cell, with Scer\GAL4^{VP16.nanos.UTR}

(Kawase et al., 2004)

maxillary palp | increased number, with Scer\GAL4^ey.PH

(Lee et al., 2007)

primary spermatocyte | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Kawase et al., 2004)

stem cell fusome, with Scer\GAL4^C587

(Kai et al., 2005)

wing disc, with Scer\GAL4^C-765

(Tanimoto et al., 2000, Burke and Basler, 1996)

Detailed Description

Statement

Reference

Overexpression of dpp^Scer\UAS.cNa driven by Scer\GAL4^dpp.PS promotes germ line stem cell proliferation in both young and aged germaria. There is a significant increase of egg production at day 3 after eclosion as a result of dpp^Scer\UAS.cNa overexpression. However, the number of eggs produced by these females rapidly drops to comparable levels as that of the control flies at day 15 and thereafter indicating that there is no extension of the reproductive life span.

(Zhao et al., 2008)

Expression of dpp^Scer\UAS.cNa under the control of Scer\GAL4^ey.PH leads to a reduction in the size of the eye disc, with the retinal field being enlarged at the expense of the rest of the disc. The antennal disc is broader and is partially duplicated. Adults of this genotype have heads that are significantly reduced in size, with misplaced, duplicated antennae and maxillary palps. Eyes are reduced in size and protrude from the head. The dorsal head is reduced in size but retains much of its normal appearance.

(Lee et al., 2007)

When dpp^Scer\UAS.cNa is expressed under the control of Scer\GAL4^Act5C.PI in several inner germarial sheath cells of the adult ovary, many spectrosome-containing single germ cells accumulate. The IGS cells that are expressing dpp^Scer\UAS.cNa under the control of Scer\GAL4^Act5C.PI maintain their normal morphology.

(Song et al., 2007)

Overexpression of dpp^Scer\UAS.cNa in somatic cells surrounding the female stem cell niche, driven by Scer\GAL4^C587, causes germline stem cells (GSCs) to divide and accumulate, so that the number of GSCs vastly outnumbers those found in wild-type ovarioles. As with wild-type GSCs, GSCs expressing dpp^Scer\UAS.cNa under the control of Scer\GAL4^C587, delay cytokinesis. However, these GSCs lack the associated cap cells seen in wild type. Additionally, the new fusome of dpp^Scer\UAS.cNa-expressing GSCs grows to the same size as the old fusome and do not stretch out into the "exclamation point" configuration characteristic of GSCs in contact with cap cells.

(Kai et al., 2005)

Expression of dpp^Scer\UAS.cNa in inner sheath cells under the control of Scer\GAL4^C587 blocks germ cell differentiation and results in the formation of germline stem cell-like tumours, leading to female sterility.

(Xi et al., 2005)

When dpp^Scer\UAS.cNa is driven by Scer\GAL4^C587 germ-line stem cell tumours are created in ovarioles.

(Kai and Spradling, 2004)

In the testes of dpp^Scer\UAS.cNa; Scer\GAL4^{nos.UTR.T:Hsim\VP16} adult males, the hub appears bigger with more cells, and there are slightly more single germ cells with a spectrosome around the hub cells. These testes also contain spermatogonial clusters with more than 16 germ cells.

(Kawase et al., 2004)

dpp^Scer\UAS.cNa; Scer\GAL4^C587 germaria are filled with single germ cells, each with a single spectrosome. After 3 successive days of 2 hour heat-shock treatment, the anterior half of the germaria in dpp^Scer\UAS.cNa; Scer\GAL4^hs.PB adult females is filled with single-spectrosome containing germ cells.

(Song et al., 2004)

Females expressing dpp^Scer\UAS.cNa under the control of Scer\GAL4^C587 have an increased number of primordial germ cells (PGCs) per gonad, averaging 245 +/- 92 per gonad compared to 136 +/- 26 in the wild type. 20.5 +/- of the PGCs are BrdU-positive in the mutant gonad compared to 15.8 +/- 2.0 % in the wild type. The mitotic activity of anterior PGCs is decreased in comparison to wild type, while the posterior PGCs are more mitotically active than wild type.

(Zhu and Xie, 2003)

Wing discs expressing dpp^Scer\UAS.cNa under the control of Scer\GAL4^C-765 show significant overgrowth.

(Tanimoto et al., 2000)

Eye discs expressing dpp^Scer\UAS.cNa under the control of Scer\GAL4^arm.PS appear to have a normal pattern of neural differentiation.

(Greenwood and Struhl, 1999)

When expressed via Scer\GAL4^hs.PB plus daily heat shock for 3 days germaria become tumorous. Large germline cells filling germarium regions 1 and 2a contain spectrosomes but show no evidence of cyst formation. Regions 2b and 3 show 16-cell cysts that probably derive from differentiated cystoblasts, or cysts that formed before the first heat shock. After 4-5 days of heat shock single germ line cells fill the germarium and 16 cell cysts are rarely detected. Germline cysts start to form in the most posterior part of the tumor 2 days after returning flies to normal temperatures. Many 16 cell cysts are seen by 4 days after the downshift, and must derive from tumor cells. Not all cysts contain 16 cells.

(Xie and Spradling, 1998)

When expression is driven by Scer\GAL4^C-765, even small clones induce significant over-proliferation. The large majority of cells in the outgrowth are wild-type and do not express dpp themselves.

(Burke and Basler, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^C587, dpp^UAS.cNa has female sterile phenotype, suppressible by Df(2L)s1402

(Xi et al., 2005)

Scer\GAL4^C587, dpp^UAS.cNa has neoplasia phenotype, suppressible | partially by pelo[+]/pelo¹

(Xi et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^C587, dpp^UAS.cNa has female germline stem cell phenotype, suppressible | partially by pelo[+]/pelo¹

(Xi et al., 2005)

NOT Suppressor of

Statement

Reference

dpp^UAS.cNa/Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B is a non-suppressor of female germline stem cell | progressive | heat sensitive phenotype of Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037, enok^HM05195

(Xin et al., 2013)

Other

Statement

Reference

Scer\GAL4^C587, bam^hs.PO, dpp^UAS.cNa has germline cyst | heat sensitive phenotype

(Kai and Spradling, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dpp^Scer\UAS.cNa with enok^GD4037 and enok^HM05195 under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B is unable to attenuate the decline in germline stem cell numbers.

(Xin et al., 2013)

The Df(2L)s1402 deficiency can rescue the sterility of females that express dpp^Scer\UAS.cNa under the control of Scer\GAL4^C587. When dpp^Scer\UAS.cNa is expressed under the control of Scer\GAL4^C587 in a pelo¹/+ background, 64% of ovarioles no longer show the stem cell tumour phenotype seen when dpp^Scer\UAS.cNa is expressed in a wild-type background. These ovarioles contain differentiated germline cysts, developing egg chambers and mature eggs. When dpp^Scer\UAS.cNa is expressed in a pelo¹ homozygous background, the tumour phenotype is further suppressed.

(Xi et al., 2005)

When bam^hs.PO is driven by heat shock in dpp^Scer\UAS.cNa, Scer\GAL4^C587 adults, cysts break down by a process of ring canal closure and fusome scission, de-differentiating. The multiple germ-line stem cells in each ovariole immediately and synchronously begin to differentiate after heat shock and soon generate 4-cell and 8-cell cysts. There are no single cells or cell pairs remaining at 20 h after heat shock. however by 30 h after heat shock (AHS) the cysts stop growing and start to beak down. By 40 h AHS closed ring canals and ring cell remnants are common. By 50 h AHS almost all germ cells are single.

(Kai and Spradling, 2004)

Scer\GAL4^69B-mediated expression creates dorsalised embryos that undergo normal dorsal closure. Scer\GAL4^69B-mediated expression in Df(2L)flp147E embryos causes a partially dorsalised phenotype and partial rescue of the dorsal closure phenotype.

(Riesgo-Escovar and Hafen, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of dpp^Scer\UAS.cNa under the control of Scer\GAL4^dpp.s-hc at 29^oC causes an almost complete rescue of the head capsule defects of dpp^TgR46.1/Df(2L)DTD2, dpp^Sal20 mutants. Expression at 27^oC is less effective and causes a 55% reduction in penetrance while expression at 25^oC results in a 22% reduction in penetrance.

(Stultz et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Analysed in clones after flipping out FRT cassette from progenitor with Scer\FLP1^hs.PG.

(Burke and Basler, 1996)

Exists in clones, not reported as being germ-line transmitted.

(Lecuit and Cohen, 1997, Nellen et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp^Scer\UAS.cNa

dpp^UAS.cNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Nellen

(Nellen et al., 1996)

Secondary FlyBase IDs

References (25)

Report Sections

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