FlyBase Allele Report: Dmel\shot[3]

In cultured neurons from stage 11 shot³ homozygous embryos, there are significantly more microtubules in axon shaft filopodia, as compared to controls. In both shot³ hetero- and homozygous conditions, the axonal microtubules are significantly more disorganized than in controls.

The medulla in shot³ heterozygous adults shows axon swelling with microtubule disorganization.

(Qu et al., 2019)

The microtubule organization in shot³ mutant follicle cell clones is perturbed as their apical enrichment is lost.

(Nashchekin et al., 2016)

Cultured primary neurons derived from homozygous embryos have a significantly reduced axon length compared to wild-type controls.

(Beaven et al., 2015)

shot³/shot^VV third instar larvae show overgrowth (increase in bouton number) at the neuromuscular junction.

shot³/shot^VV axons show enhanced regenerative axonal sprouting compared to controls after a crushing injury.

(Valakh et al., 2013)

shot³/Df(2R)MK1 primary neuronal cultures show a significantly reduced axonal length compared to controls. They also show an increase of neurons with unbundled microtubules - these microtubules display irregular trajectories and frequent loops in axons and growth cones.

Scer\GAL4^sca-537.4-mediated expression of shot^{LC.Scer\UAS.T:Avic\GFP} fails to restore the microtubule unbundling and axonal length phenotypes of shot³/Df(2R)MK1 primary neuronal cultures.

shot³/Df(2R)MK1 primary neuronal cultures show a significant reduction in filopodia number.

Scer\GAL4^sca-537.4-mediated expression of shot^{LA-ΔEF-hand.Scer\UAS.T:Avic\GFP} fails to fully rescue the reduced filopodia phenotype of shot³/Df(2R)MK1 primary neuronal cultures.

(Sanchez-Soriano et al., 2009)

In shot³ embryos, the pCC axon aberrantly crosses the midline in 16% of CNS segments.

(Lee et al., 2007)

Homozygous germ-line clones produce egg chambers, but they do not progress beyond stage 7 of oogenesis. Each mutant egg chamber contains 16 nuclei, and the pattern of divisions is also normal. Fusome structure and persistence appear normal. In addition the fusome/spectrosome is still distributed asymmetrically in early stem cell/cystoblast division, with the larger portion being found in the stem cell after division. In mutant cysts all of the nuclei, including the two at the posterior of the egg chamber appear polyploid: All of the germ-line cells adopt a nurse cell fate, no oocyte is specified. The synaptonemal complex accumulates in none or two or more cells all throughout region 2a of the cyst, but this accumulation fails to refine onto one cell only and is always lost region 2b. In wild-type ovaries, microtubules are highly concentrated around the fusome from region 2a on, and in region 2b and 3 a microtubule organising centre (MTOC) is visible in the oocyte. This organisation and concentration of microtubules is lost in mutant germ-line clones; microtubules fail to concentrate around the fusome., and an MTOC does not form in region 2b or 3. the migration of the centrosomes into the oocyte normally seen in wild-type ovaries fails to occur.

(Roper and Brown, 2004)

Mutant mushroom body neuroblast clones examined in the adult show abnormal processes projecting out from the calyx. Mutant mushroom body neuroblast clones have a significant reduction in cell number compared to control clones.

(Reuter et al., 2003)

shot³ germline clones do not produce viable eggs. shot³ clones of cells are often double-layered in egg chambers from stage 6-7 onwards, with actin accumulation at the contacts between the two layers. Double-layered clones are only detected in cells overlying the oocyte, and at stage 10 of oogenesis are generally found in the posterior half of the follicle cells covering the oocyte. Microtubule levels and organisation, integrin localisation and oocyte elongation are normal in shot³ mutant cells.

(Roper and Brown, 2003)

Mutant embryos show defects in motor axon extension; the ISN stalls at the approximate position where it would normally make the second dorsalmost muscle contact, the SNa stalls where it would normally bifurcate and the ISNb stalls in the ventral muscle field and fails to reach muscle 12. Mutant embryos also show defects in sensory axon extension.

(Lee and Kolodziej, 2002)

The tracheal dorsal trunk lumen is discontinuous at 75% of anastomosis sites in stage 14 or older mutant embryos. Lateral trunk connections and all anastomoses at the dorsal midline are affected. The overall branching pattern of trachea within hemisegments appears normal. 6% of tracheal dorsal trunk connections form apparently normally and 19% are constricted relative to wild type. Fusion cells appear to be present at the dorsal trunk anastomosis sites. Microtubules are not apically located at anastomosis sites of the tracheal dorsal trunk in mutant embryos, in contrast to wild type, though they remain largely at the cell periphery. F-actin accumulates apically in the tracheal cells but the lumen does not extend through the fusion cells at the anastomosis site.

(Lee and Kolodziej, 2002)

shot³ mutants show strong reduction of output synapses at the NMJ, while this phenotype is not seen in the CNS.

(Lohr et al., 2002)

The lateral chordotonal sensory axons arrest at variable stages before reaching the central nervous system (CNS); 93% extend towards the intersegmental nerve (ISN) and stall or fail to reach the ISN, 7% travel a significant distance within the ISN, but none of the axons reach the CNS border. The morphology of the LCH growth cones is normal, with the growth cone adopting the general morphology of wild-type growth cones at the place where they stall. Defects in LCH dendrite morphology are seen in shot³ and shot²/shot³ mutant embryos. 90-100% of the motor axons of all three major pathways (ISN, SNa and ISNb) stall prematurely in late stage 16/stage 17 homozygous embryos. 3% of ISN axons lack the most dorsal terminal arborisation, 68% lack the 2 most dorsal terminal arborisations and 24% lack all three terminal arborisations. 1% of ISN axons stall before entering the dorsal muscle field. The SNa and ISNb motor axons stall before reaching their muscle targets. 75% of ISNb motor axons stall in the ventral muscle field (somewhere between muscle 6 and muscle 13) and 14% stall at the muscle field border. 33% of SNa axons have one branch missing (wild-type axons form two branches), 38% lack both branches and 17% stall over the ventral muscles or are absent. Some SNa and ISNb motor axon bundles fail to exit the CNS successfully or stall earlier than the muscle field entry points in shot³/Df(2R)CX1 embryos. 61% of ISNb motor axons stall in the ventral muscle field (somewhere between muscle 6 and muscle 13) and 20% stall at the muscle field border. 40% of ISN axons lack the 2 most dorsal terminal arborisations and 42% lack all three terminal arborisations. 17% of ISN axons stall before entering the dorsal muscle field. 24% of SNa axons have one branch missing (wild-type axons form two branches), 60% lack both branches and 8% stall over the ventral muscles or are absent. 6% of ISN axons lack the most dorsal terminal arborisation, 48% lack the 2 most dorsal terminal arborisations and 43% lack all three terminal arborisations in shot²/shot³ embryos. 57% of ISNb motor axons stall in the ventral muscle field (somewhere between muscle 6 and muscle 13) and 30% stall at the muscle field border. 46% of SNa axons have one branch missing (wild-type axons form two branches) and 28% lack both branches. Muscle morphology and number is normal in late stage 16 homozygous embryos.

(Lee et al., 2000)

Mushroom body neuroblast clones induced using the MARCM system exhibit drastic axon projection defects compared with wild type controls. The origin of the pathfinding errors appears to be near the dendritic projection area. In the majority of cases the abnormal axon bundles do project in a generally wild type direction, towards the midline region. Some even eventually fuse with the medial lobe, as they do in the wild type.

(Lee and Luo, 1999)

Mutant phenotype as assayed by Ecol\lacZ^rp staining: longitudinal connectives missing. Mutant phenotype of lateral chordotonal axons includes: missing axons.

(Kolodziej et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

shot³/shot[+], sli² has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

shot³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

shot³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by kra²/kra¹

(Lee et al., 2007)

robo1², shot³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

Suppressed by

Statement

Reference

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by wnd[+]/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wnd³/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Valakh et al., 2013)

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Valakh et al., 2013)

Enhancer of

Statement

Reference

shot³ is an enhancer of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

shot³/shot[+] is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of sli²

(Lee et al., 2007)

shot³ is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of robo1²

(Lee et al., 2007)

shot³/shot[+] is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of kra²/kra¹

(Lee et al., 2007)

Suppressor of

Statement

Reference

shot³/shot[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

shot³/shot[+] is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

shot³ has terminal tracheal cell | embryonic stage phenotype, enhanceable by tau^MR22

(Ricolo and Araujo, 2020)

shot³ has tracheal lumen | embryonic stage phenotype, enhanceable by tau^MR22

(Ricolo and Araujo, 2020)

shot³ has axon | embryonic stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^elav.PLu

(Qu et al., 2019)

shot³ has filopodium | embryonic stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^elav.PLu

(Qu et al., 2019)

shot³ has microtubule | embryonic stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^elav.PLu

(Qu et al., 2019)

shot³ has axon microtubule bundle | embryonic stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^elav.PLu

(Qu et al., 2019)

shot³ has axon | adult stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^GMR31F10

(Qu et al., 2019)

shot³ has medulla | adult stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^GMR31F10

(Qu et al., 2019)

shot³ has axon microtubule bundle | adult stage phenotype, enhanceable by Efa6^GD14945/Scer\GAL4^GMR31F10

(Qu et al., 2019)

shot³ has axon microtubule bundle | embryonic stage phenotype, enhanceable by Efa6^GX6w-

(Qu et al., 2019)

shot³/shot[+], sli² has axon & pCC neuron phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

shot³ has axon & pCC neuron phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

shot³ has axon & pCC neuron phenotype, enhanceable by kra²/kra¹

(Lee et al., 2007)

robo1², shot³ has axon & pCC neuron phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

NOT Enhanced by

Statement

Reference

shot³ has axon microtubule bundle | embryonic stage phenotype, non-enhanceable by Eb1⁰⁴⁵²⁴/Eb1⁰⁴⁵²⁴

(Hahn et al., 2021)

Suppressed by

Statement

Reference

shot³ has tracheal lumen | embryonic stage phenotype, suppressible | partially by Scer\GAL4^btl.PU/Avic\GFP^{UASp.Tag:MT(btau)}

(Ricolo and Araujo, 2020)

shot³ has terminal tracheal cell | embryonic stage phenotype, suppressible | partially by Scer\GAL4^btl.PU/Scer\GAL4^btl.PU/Avic\GFP^{UASp.Tag:MT(btau)}/Avic\GFP^{UASp.Tag:MT(btau)}

(Ricolo and Araujo, 2020)

shot³ has microtubule | embryonic stage phenotype, suppressible | partially by Scer\GAL4^btl.PU/Scer\GAL4^btl.PU/Avic\GFP^{UASp.Tag:MT(btau)}/Avic\GFP^{UASp.Tag:MT(btau)}

(Ricolo and Araujo, 2020)

shot³ has actin cytoskeleton | embryonic stage phenotype, suppressible | partially by Scer\GAL4^btl.PU/Scer\GAL4^btl.PU/Avic\GFP^{UASp.Tag:MT(btau)}/Avic\GFP^{UASp.Tag:MT(btau)}

(Ricolo and Araujo, 2020)

shot³ has axon microtubule bundle | embryonic stage phenotype, suppressible by Efa6^UAS.EGFP/Scer\GAL4^elav.PLu

(Qu et al., 2019)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible | partially by wnd[+]/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by wnd³/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Valakh et al., 2013)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Valakh et al., 2013)

NOT suppressed by

Statement

Reference

shot³ has axon phenotype, non-suppressible by CLIP-190^KO/CLIP-190^KO

(Beaven et al., 2015)

Enhancer of

Statement

Reference

shot³/shot³ is an enhancer of axon | embryonic stage phenotype of Efa6^GX6w-

(Qu et al., 2019)

shot³/shot³ is an enhancer of filopodium | embryonic stage phenotype of Efa6^GX6w-

(Qu et al., 2019)

shot³/shot³ is an enhancer of microtubule | embryonic stage phenotype of Efa6^GX6w-

(Qu et al., 2019)

shot³/shot³ is an enhancer of axon microtubule bundle | embryonic stage phenotype of Efa6^GX6w-

(Qu et al., 2019)

shot³/shot[+] is an enhancer of axon microtubule bundle | embryonic stage phenotype of Efa6^GD14945, Scer\GAL4^elav.PLu

(Qu et al., 2019)

shot³/shot[+] is an enhancer of axon | adult stage phenotype of Efa6^GD14945, Scer\GAL4^GMR31F10

(Qu et al., 2019)

shot³/shot[+] is an enhancer of medulla | adult stage phenotype of Efa6^GD14945, Scer\GAL4^GMR31F10

(Qu et al., 2019)

shot³/shot[+] is an enhancer of axon microtubule bundle | adult stage phenotype of Efa6^GD14945, Scer\GAL4^GMR31F10

(Qu et al., 2019)

shot³ is an enhancer of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

shot³/shot[+] is an enhancer of axon & pCC neuron phenotype of sli²

(Lee et al., 2007)

shot³ is an enhancer of axon & pCC neuron phenotype of robo1²

(Lee et al., 2007)

shot³/shot[+] is an enhancer of axon & pCC neuron phenotype of kra²/kra¹

(Lee et al., 2007)

NOT Enhancer of

Statement

Reference

shot³/shot³ is a non-enhancer of axon microtubule bundle | embryonic stage phenotype of Eb1⁰⁴⁵²⁴

(Hahn et al., 2021)

Suppressor of

Statement

Reference

shot³/shot[+] is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

shot³/shot[+] is a suppressor of embryonic head phenotype of mira^{αTub67C.mGFP6}

(Chang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in axon length seen in cultured primary neurons derived from homozygous shot³ embryos is unchanged if the embryos are also homozygous for CLIP-190^KO.

(Beaven et al., 2015)

wnd²/+ partially suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

wnd²/wnd³ fully suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

Expression of either kay^Fbz.Scer\UAS or bsk^{K53R.Scer\UAS} under the control of Scer\GAL4^elav.PU suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

(Valakh et al., 2013)

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is suppressed if the females are also heterozygous for shot³.

(Chang et al., 2011)

The axon midline phenotype of sli²/+ embryos is enhanced in sli²/+; exba²/+ and shot³/+, sli²/+ double mutants. The phenotype is further enhanced in shot³/+, sli²/+; exba²/+ triple mutants.

(Lee et al., 2007)

The frequency of aberrant pCC axon midline crossovers in shot³/+; exba¹/exba² and shot³/shot³; exba²/+ double mutant embryos is significantly greater than in either single mutant homozygous embryos. The midline crossing phenotype is slightly more severe in shot³/shot³; exba¹/exba² embryos compared to shot³/shot³; exba²/+ embryos, while shot³/+; exba²/+ embryos show no significant midline defects.

The pCC axon midline crossing phenotype of robo² embryos is dramatically enhanced in robo²/+; exba²/+ embryos and in shot³, robo²/+ embryos. The phenotype is further enhanced in shot³, robo²/+; exba²/+ embryos.

(Lee et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

shot⁶

(Reuter et al., 2003)

shot⁷

(Reuter et al., 2003)

shot⁸

(Reuter et al., 2003)

Rescued by

shot³ is rescued by Scer\GAL4^btl.PU/shot^LA.UAS.GFP

(Ricolo and Araujo, 2020)

shot^VV/shot³ is rescued by Scer\GAL4^elav.PU/shot^LA.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is rescued by Scer\GAL4^elav.PU/shot^{LA-ΔPlakin.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is rescued by Scer\GAL4^elav.PU/shot^{LA-Δrod1.UAS.GFP}

(Valakh et al., 2013)

shot³/Df(2R)MK1 is rescued by shot^LC.UAS.GFP/Scer\GAL4^sca-537.4

(Sanchez-Soriano et al., 2009)

shot³/Df(2R)MK1 is rescued by shot^{LA-ΔEF-hand.UAS.GFP}/Scer\GAL4^sca-537.4

(Sanchez-Soriano et al., 2009)

shot³ is rescued by Scer\GAL4^insc-Mz1407/shot^LA.UAS.GFP

(Lee et al., 2007)

shot³ is rescued by Scer\GAL4^insc-Mz1407/shot^{LA-ΔGAS2.UAS.GFP}

(Lee et al., 2007)

shot³ is rescued by Scer\GAL4^insc-Mz1407/shot^LB.UAS.GFP

(Lee and Kolodziej, 2002)

shot³ is rescued by Scer\GAL4^insc-Mz1407/shot^LA.UAS.GFP

(Lee and Kolodziej, 2002)

shot³ is rescued by Scer\GAL4^insc-Mz1407/shot^{LA-Δrod1.UAS.GFP}

(Lee and Kolodziej, 2002)

shot³ is rescued by shot^{LA-ΔGAS2.UAS.GFP}/Scer\GAL4^btl.PS

(Lee and Kolodziej, 2002)

shot³ is rescued by shot^LC.UAS.GFP/Scer\GAL4^btl.PS

(Lee and Kolodziej, 2002)

shot³ is rescued by shot^LA.UAS.GFP/Scer\GAL4^NP5130

(Lee and Kolodziej, 2002)

shot³ is rescued by Scer\GAL4^btl.PS/shot^LA.UAS.GFP

(Lee and Kolodziej, 2002)

Partially rescued by

shot³/Df(2R)MK1 is partially rescued by shot^{LA-ΔEF-hand.UAS.GFP}/Scer\GAL4^sca-537.4

(Sanchez-Soriano et al., 2009)

shot³ is partially rescued by Scer\GAL4^insc-Mz1407/shot^{LA-ΔEF-hand.UAS.GFP}

(Lee et al., 2007)

shot³ is partially rescued by Scer\GAL4^insc-Mz1407/shot^{LA-ΔEF-hand.UAS.GFP}

(Lee and Kolodziej, 2002)

Not rescued by

shot³ is not rescued by Scer\GAL4^btl.PU/shot^{Ctail.UAS.GFP}

(Ricolo and Araujo, 2020)

shot³ is not rescued by Scer\GAL4^btl.PU/shot^LC.UAS.GFP

(Ricolo and Araujo, 2020)

shot³ is not rescued by Scer\GAL4^btl.PU/shot^{RE-ΔCtail.UAS.GFP}

(Ricolo and Araujo, 2020)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^LA.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^elav.PU/shot^LC.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^LC.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^elav.PU/shot^{LA-ΔEF-hand.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^{LA-ΔEF-hand.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by shot^{LA-ΔPlakin.UAS.GFP}/Scer\GAL4^how-24B

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^{LA-Δrod1.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^elav.PU/shot^{LA-ΔGAS2.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^{LA-ΔGAS2.UAS.GFP}

(Valakh et al., 2013)

shot³/Df(2R)MK1 is not rescued by shot^LC.UAS.GFP/Scer\GAL4^sca-537.4

(Sanchez-Soriano et al., 2009)

shot³ is not rescued by Scer\GAL4^insc-Mz1407/shot^LC.UAS.GFP

(Lee et al., 2007)

shot³ is not rescued by Scer\GAL4^insc-Mz1407/shot^LC.UAS.GFP

(Lee and Kolodziej, 2002)

shot³ is not rescued by Scer\GAL4^insc-Mz1407/shot^{LA-ΔGAS2.UAS.GFP}

(Lee and Kolodziej, 2002)

shot³ is not rescued by shot^{LC-ΔGAS2.UAS.GFP}/Scer\GAL4^btl.PS

(Lee and Kolodziej, 2002)

Comments

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae, while expression under the control of Scer\GAL4^how-24B fails to rescue the phenotype.

Expression of either shot^{LA-ΔPlakin.Scer\UAS.T:Avic\GFP} or shot^{LA-Δrod1.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

Expression of either shot^{LC.Scer\UAS.T:Avic\GFP}, shot^{LA-ΔEF-hand.Scer\UAS.T:Avic\GFP} or shot^{LA-ΔGAS2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

(Valakh et al., 2013)

Scer\GAL4^sca-537.4-mediated expression of shot^{LC.Scer\UAS.T:Avic\GFP} fails to restore the microtubule unbundling and axonal length phenotypes of shot³/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4^sca-537.4-mediated expression of shot^{LA-ΔEF-hand.Scer\UAS.T:Avic\GFP} fully restores the microtubule unbundling and axonal length phenotypes of shot³/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4^sca-537.4-mediated expression of shot^{LC.Scer\UAS.T:Avic\GFP} fully rescues the reduced filopodia phenotype of shot³/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4^sca-537.4-mediated expression of shot^{LA-ΔEF-hand.Scer\UAS.T:Avic\GFP} fails to fully rescue the reduced filopodia phenotype of shot³/Df(2R)MK1 primary neuronal cultures.

(Sanchez-Soriano et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

5141

w^*; P{FRT(w^hs)}G13 shot³/CyO, P{ase-lacZF:2.0}PK2

29649

w^*; P{UAS-shot.L(A)-ΔPlakin-GFP}2, shot³/CyO, P{en1}wg^en11

Kyoto

108072