P{EP} insertion is in reverse orientation relative to the direction of transcription.
visible (with apP44), with Scer\GAL4ap-md544, apΔHD.UAS
visible (with apts78j), with Scer\GAL4ap-md544, apΔHD.UAS
wing (with apP44), with Scer\GAL4ap-md544, apUAS.cOa
wing (with apP44), with Scer\GAL4ap-md544, apΔHD.UAS
wing (with apts78j), with Scer\GAL4ap-md544, apHA-B.UAS
wing (with apts78j), with Scer\GAL4ap-md544, apΔHD.UAS
wing | heat sensitive (with apts78j)
wing margin (with apUGO35)
apmd544/+ imaginal discs show random apoptosis in the wing pouch that is higher in the dorsal region of the developing wing, but that does not seem to affect the morphology of the adult, because those wings are morphologically normal.
In apUGO35/apmd544 and in apmd544/Df(2R)nap1 flies, the fourth tarsal segment is almost completely lost. The remnant is fused to the fifth tarsal segment. Other segments are normal.
Neurons which normally express ap (Ap neurons) are still present and extend axons in apP44/apmd544 embryonic brains. However, Ap brain interneurons show defasciculation and pathfinding defects in these animals. The most severe defects are seen in the deutocerebrum, where the two pairs of Ap neuronal cell bodies are positioned incorrectly. These neurons do not appear to undergo the movements seen in wild-type embryos and never become localised on the inner surface of the developing deutocerebrum, instead remaining on the outer surface, with their axons projecting aberrantly along the frontal commissure of the stomatogastric nervous system or in rare cases along the recurrent nerve. The cell bodies of the Ap neurons in the protocerebrum appear to be positioned properly and have a normal size and shape, but many of their commissural axons fail to form commissural fascicles correctly and often leave their commissural bundle and cross over to grow along a neighbouring fascicle. This defect is seen in 30% of cases. Ap neurons that innervate the ring gland have normal axonal projection patterns. Fas2-expressing commissural fascicles are indistinguishable from wild type.
Transheterozygotes with Df(2R)nap1 show fusion of tarsus 4 with 5, reduction and deformity or loss of tarsus 4. Tarsus four and five fusions appear with lower frequency and expressivity in apUGO35/apmd544 transheterozygotes.
apmd544/apUGO35 flies have immature ovaries, are sterile, die within 24-72 hours of eclosion and have no wings. apmd544/apts78j flies grown at 25oC have wings that lack most of the wing margin but have good proximo-distal growth. apmd544/apts78j flies grown at 25oC have wings that lack most of the wing margin but have good proximo-distal growth. The addition of apHA-B.Scer\UAS severely reduces proximo-distal growth and wing size.
In apmd544/apP44 embryos, all neurons that normally express ap survive and extend axons, but these axons display pathfinding errors in nearly every segment, including defasciculation of axon bundles, aberrant pathway selection within the longitudinal connectives, occasional misrouting across the midline and stalling.
apmd544/apP44 flies have no wings, although they often have a ribbon-like outgrowth in the wing region that lacks any recognisable structures. Homozygous adults are sterile, uncoordinated and have a reduced lifespan. Axon pathfinding errors are seen in the central nervous system of apmd544/apP44 embryos; neurons that normally express ap wander within the longitudinal connective and fail to fasciculate with one another. apmd544/apts78j flies have defective wing margins and unfused wing surfaces at 18oC. Introduction of apΔHD.Scer\UAS into this background (expressed under the control of Scer\GAL4ap-md544) eliminates the wing blade altogether.
apmd544 has lethal | recessive phenotype, suppressible by Scer\GAL4ap-md544/lncRNA:HsrωRNAi.Sym.UAS/ lncRNA:Hsromega [+]
apmd544 has lethal | recessive phenotype, suppressible by Scer\GAL4ap-md544, lncRNA:HsrωRNAi.Sym.UAS/lncRNA:HsrωRNAi.Sym.UAS
apmd544 has increased cell death | dominant | third instar larval stage phenotype, suppressible by Scer\GAL4ap-md544/Diap1UAS.cHa
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by cicEP3-1005/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/EP3-1199EP3-1199
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/taraEP3-1575
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/nufEP3-1715
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by EP3-1729EP3-1729/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/EP3-1789EP3-1789
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by EP3-1809EP3-1809/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/Su(Tpl)EP3-2015
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by SlhEP2-18/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by lilliEP2-27/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-235
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-356
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-402
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by cbtEP2-408/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/EP2-430EP2-430
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by nmdEP2-446
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by sbbEP2-473/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/EP2-760EP2-760
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-814
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/CG8405EP2-865
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by drprEP2-867
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/chmEP2-1069
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/EP2-1080EP2-1080
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by mir-14EP2-1142/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/shnEP2-1279
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/apEP2-1583
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by shnEP2-1743/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by mir-282EP3-26/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/Pcif1EP3-28
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by mir-279EP3-364/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/EP3-378EP3-378
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-473
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/Mpcp2EP3-488
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/fngEP3-511
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by skdEP3-532
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/AnxB9EP3-562
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/fngEP3-581
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/EP3-612EP3-612
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by EP3-934EP3-934/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-619
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-900
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/osaEP3-941
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by osaEP3-1074/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-1386
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by osaEP3-1591/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by pntEP3-853/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/pntEP3-1638
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by pntEP3-1859/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by Scer\GAL4ap-md544/14-3-3εEP3-732
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/CG1943EP3-797
Bx1, ap[+]/apmd544 has visible phenotype, suppressible by CG8369EP3-826/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/EP3-980EP3-980
apmd544/apts78j has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/mewUAS.cWa/fngUAS.cKa
aprK568/apmd544 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/fngUAS.cKa
apmd544/apUGO35 has visible phenotype, suppressible by Scer\GAL4ap-md544/ap::BxdLAp.UAS.Tag:FLAG
apmd544/apP44 has visible phenotype, suppressible by ap::ChiChAp.UAS/Scer\GAL4ap-md544
apmd544/apUGO35 has visible phenotype, suppressible by Scer\GAL4ap-md544/SerUAS.cSa
apmd544/apUGO35 has visible phenotype, suppressible by fngEP3082/Scer\GAL4ap-md544
apmd544/apUGO35 has visible phenotype, suppressible by Scer\GAL4ap-md544/Hsap\LHX2UAS.cRa
apmd544/apUGO35 has sterile phenotype, suppressible by Scer\GAL4ap-md544/Hsap\LHX2UAS.cRa
apmd544/apUGO35 has short lived phenotype, suppressible by Scer\GAL4ap-md544/Hsap\LHX2UAS.cRa
apmd544/apP44 has visible phenotype, suppressible | partially by Scer\GAL4ap-md544/ap::ChiΔLID.ΔLIM.UAS.Tag:MYC
apmd544/apP44 has visible phenotype, suppressible | partially by Lim3UAS.cOa/Scer\GAL4ap-md544
apmd544 has increased cell death | dominant | third instar larval stage phenotype, non-suppressible by SPARCUASp.cPa/Scer\GAL4ap-md544
apmd544/apP44 has visible phenotype, non-suppressible by Scer\GAL4ap-md544/ChiΔLID.UAS.Tag:MYC
Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35, fngEP3082 has visible phenotype
Hsap\LHX2UAS.cRa, Scer\GAL4ap-md544, apmd544/apUGO35 has visible phenotype
Hsap\LHX2UAS.cRa, Scer\GAL4ap-md544, apmd544 has visible | recessive phenotype
apmd544 has wing disc | third instar larval stage phenotype, suppressible by Scer\GAL4ap-md544/Diap1UAS.cHa
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by SlhEP2-18/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by lilliEP2-27/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-235
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-356
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-402
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by cbtEP2-408/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/EP2-430EP2-430
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by nmdEP2-446
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by sbbEP2-473/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/EP2-760EP2-760
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/mir-14EP2-814
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/CG8405EP2-865
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by drprEP2-867
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/chmEP2-1069
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/EP2-1080EP2-1080
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by mir-14EP2-1142/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/shnEP2-1279
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/apEP2-1583
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by shnEP2-1743/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by mir-282EP3-26/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/Pcif1EP3-28
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by mir-279EP3-364/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/EP3-378EP3-378
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-473
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/Mpcp2EP3-488
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/fngEP3-511
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by skdEP3-532
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/AnxB9EP3-562
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/fngEP3-581
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/EP3-612EP3-612
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by EP3-934EP3-934/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-619
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-900
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/osaEP3-941
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by osaEP3-1074/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/osaEP3-1386
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by osaEP3-1591/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by pntEP3-853/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/pntEP3-1638
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by pntEP3-1859/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/14-3-3εEP3-732
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/CG1943EP3-797
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by CG8369EP3-826/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/EP3-980EP3-980
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by cicEP3-1005/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/EP3-1199EP3-1199
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/taraEP3-1575
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/nufEP3-1715
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by EP3-1729EP3-1729/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/EP3-1789EP3-1789
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by EP3-1809EP3-1809/Scer\GAL4ap-md544
Bx1, ap[+]/apmd544 has wing margin phenotype, suppressible by Scer\GAL4ap-md544/Su(Tpl)EP3-2015
apmd544/apUGO35 has tarsal segment 4 phenotype, suppressible by Scer\GAL4ap-md544/ap::Lim3UAS.cOa
apmd544/apUGO35 has pretarsus phenotype, suppressible by Scer\GAL4ap-md544/ap::ChiΔLID.ΔLIM.UAS.Tag:MYC
apmd544/apUGO35 has pretarsus phenotype, suppressible by Scer\GAL4ap-md544/ap::Lim3UAS.cOa
apmd544/apUGO35 has tarsal segment 4 phenotype, suppressible by Scer\GAL4ap-md544/ap::ChiΔLID.ΔLIM.UAS.Tag:MYC
apmd544 has wing disc phenotype, suppressible by fngUAS.B.Tag:MYC/Scer\GAL4ap-md544
apmd544 has wing disc phenotype, suppressible by fngEP3082/Scer\GAL4ap-md544
aprK568/apmd544 has wing disc phenotype, suppressible | partially by fngUAS.B.Tag:MYC/Scer\GAL4ap-md544
aprK568/apmd544 has dorsal-ventral compartment boundary of the wing disc phenotype, suppressible by fngUAS.B.Tag:MYC/Scer\GAL4ap-md544
apmd544/apUGO35 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/DrUAS.cIa
apmd544/apUGO35 has wing margin phenotype, suppressible | partially by fngEP3082/Scer\GAL4ap-md544
apmd544/apts78j has wing phenotype, suppressible | partially by Scer\GAL4ap-md544/mewUAS.cWa/fngUAS.cKa
aprK568/apmd544 has wing disc phenotype, suppressible by Scer\GAL4ap-md544/fngUAS.cKa
aprK568/apmd544 has wing margin phenotype, suppressible | partially by Scer\GAL4ap-md544/fngUAS.cKa
aprK568/apmd544 has wing phenotype, suppressible | partially by Scer\GAL4ap-md544/fngUAS.cKa
apmd544/apUGO35 has wing phenotype, suppressible by Scer\GAL4ap-md544/ap::BxdLAp.UAS.Tag:FLAG
Chie5.5, apmd544/apP44 has embryonic/larval neuron phenotype, suppressible by Scer\GAL4ap-md544/ap::ChiΔLID.ΔLIM.UAS.Tag:MYC
apmd544/apP44 has wing phenotype, suppressible by ap::ChiChAp.UAS/Scer\GAL4ap-md544
apmd544/apUGO35 has wing phenotype, suppressible by Scer\GAL4ap-md544/SerUAS.cSa
apmd544/apUGO35 has wing margin bristle phenotype, suppressible by Scer\GAL4ap-md544/SerUAS.cSa
apmd544/apUGO35 has wing phenotype, suppressible by fngEP3082/Scer\GAL4ap-md544
apmd544/apUGO35 has wing margin bristle phenotype, suppressible by fngEP3082/Scer\GAL4ap-md544
apmd544/apUGO35 has wing phenotype, suppressible by Scer\GAL4ap-md544/Hsap\LHX2UAS.cRa
apmd544/apUGO35 has haltere phenotype, suppressible by Scer\GAL4ap-md544/Hsap\LHX2UAS.cRa
apmd544/apUGO35 has scutellum phenotype, suppressible by Scer\GAL4ap-md544/Hsap\LHX2UAS.cRa
apmd544/apP44 has wing phenotype, suppressible | partially by Scer\GAL4ap-md544/ap::ChiΔLID.ΔLIM.UAS.Tag:MYC
apmd544/apP44 has wing phenotype, suppressible | partially by Lim3UAS.cOa/Scer\GAL4ap-md544
apmd544/apP44 has larval longitudinal connective phenotype, suppressible | partially by Lim3UAS.cOa/Scer\GAL4ap-md544
apmd544/apP44 has wing phenotype, suppressible | partially by Scer\GAL4ap-md544/ap::Lim3UAS.cOa
apmd544/apP44 has larval longitudinal connective phenotype, suppressible | partially by Scer\GAL4ap-md544/ap::Lim3UAS.cOa
apmd544 has wing disc | third instar larval stage phenotype, non-suppressible by SPARCUASp.cPa/Scer\GAL4ap-md544
apmd544/apUGO35 has wing disc phenotype, non-suppressible by fngUAS.B.Tag:MYC/Scer\GAL4ap-md544
apmd544/apUGO35 has wing disc phenotype, non-suppressible by fngEP3082/Scer\GAL4ap-md544
apmd544/apUGO35 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by Scer\GAL4ap-md544/ConUAS.cRa
apmd544/apUGO35 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by fngEP3082/Scer\GAL4ap-md544
aprK568/apmd544 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by fngEP3082/Scer\GAL4ap-md544
apmd544/apUGO35 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by Scer\GAL4ap-md544/capsUAS.cSa
apmd544/apUGO35 has wing phenotype, non-suppressible by Scer\GAL4ap-md544/DrUAS.cIa
apmd544/apUGO35 has pretarsus phenotype, non-suppressible by ChiFL.UAS.Tag:MYC/Scer\GAL430A
apmd544/apUGO35 has tarsal segment 4 phenotype, non-suppressible by ChiFL.UAS.Tag:MYC/Scer\GAL430A
apmd544/apP44 has phenotype, non-suppressible by apΔLIM.UAS/Scer\GAL4ap-md544/ChiΔLID.UAS.Tag:MYC
apmd544/apP44 has phenotype, non-suppressible by Scer\GAL4ap-md544/ChiΔLID.UAS.Tag:MYC
apmd544/apP44 has wing phenotype, non-suppressible by Scer\GAL4ap-md544/ChiΔLID.UAS.Tag:MYC
ap[+]/apmd544 is an enhancer of wing margin phenotype of Bx1
Scer\GAL4ap-md544, aprK568/apmd544, fngUAS.cKa has haltere phenotype
Bx1, ap[+]/apmd544 has wing margin phenotype
Scer\GAL4ap-md544, apmd544/apUGO35, fngEP3082 has wing margin | anterior | dorsal phenotype
Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has wing vein phenotype
Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has dorsal row of wing sensilla phenotype
Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has wing margin phenotype
Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has alula & macrochaeta | supernumerary phenotype
Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has sensillum campaniformium | supernumerary & wing vein L3 | ventral phenotype
Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has ventral row of wing sensilla | increased number phenotype
Scer\GAL4ap-md544, aprK568/apmd544, fngUAS.cKa has dorsal row of wing sensilla phenotype
Scer\GAL4ap-md544, aprK568/apmd544, fngUAS.cKa has ventral row of wing sensilla | increased number phenotype
Scer\GAL4ap-md544, apmd544/apUGO35, fngEP3082 has wing disc phenotype
Scer\GAL4ap-md544, apmd544/apUGO35, fngEP3082 has wing margin bristle | ectopic phenotype
Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35, fngEP3082 has wing phenotype
Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35 has wing disc phenotype
Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35 has wing margin bristle | ectopic phenotype
Overexpression of BM-40-SPARCScer\UAS.cPa under the control of Scer\GAL4ap-md544 fails to suppress the increased apoptosis in apmd544/+ wing discs.
Overexpression of thScer\UAS.cHa under the control of Scer\GAL4ap-md544 suppresses the increased apoptosis in apmd544/+ wing discs.
Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 in a aprK568/apmd544 background have ventralised halteres. In addition, the halteres form dark-pigmented bristles that are similar to those formed at the wing margin.
apmd544/+ does not cause any visible leg phenotypes in combination with Chig371/+, Chig96.1/+ or Chie5.5/+. ap::ChiΔLID.ΔLIM.Scer\UAS.T:Hsap\MYC; Scer\GAL4ap-md544 suppresses fusion of tarsal segments 4 and 5 in apUGO35/apmd544 flies. ap::Lim3Scer\UAS.cOa; Scer\GAL4ap-md544 suppresses fusion of tarsal segments 4 and 5 in apUGO35/apmd544 flies.
Expression of capsScer\UAS.cSa under the control of Scer\GAL4ap-md544 restores the smooth interface between dorsal and ventral cells in apmd544/apUGO35 wing discs. The boundary between the dorsal and ventral cells is still irregular in apmd544/apUGO35 wing discs expressing trnScer\UAS.cMa under the control of Scer\GAL4ap-md544. Expression of ConScer\UAS.cRa or fngEP3082 under the control of Scer\GAL4ap-md544 does not restore a smooth boundary between the dorsal and ventral cells in apmd544/apUGO35 wing discs.
Expression of DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Growth of the wing and wing margin formation can be restored in apmd544/apUGO35 flies expressing fngEP3082 under the control of Scer\GAL4ap-md544. Both surfaces of the wing differentiate ventral structures; the anterior wing margin and alula differentiate ventral bristles on both surfaces and the pattern of vein corrugation is ventral. Co-expression of fngEP3082 and DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin.
A sharp boundary between dorsal and ventral cells is restored in aprK568/apmd544 wing discs expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544.
No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure.
apUGO35/apmd544 flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 do not have wing margin defects.
Coexpression of mewScer\UAS.cWa and fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 partially rescues the apts78j/apmd544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristles types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they are entirely of ventral identity (the veins show a ventral corrugation pattern on both surfaces of the wing).
A sharp boundary between dorsal and ventral cells is restored in aprK568/apmd544 wing discs expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544. No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure. apUGO35/apmd544 flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 do not have wing margin defects. Coexpression of mewScer\UAS.cWa and fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 partially rescues the apts78j/apmd544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristles types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity (the veins show a ventral corrugation pattern on both surfaces of the wing).
No increase in axon guidance defects is seen in apmd544/Chie5.5 transheterozygotes compared to apmd544/+ or Chie5.5/+ embryos. ap::ChiΔLID.ΔLIM.Scer\UAS.T:Hsap\MYC rescues pathway recognition and axon fasciculation in a majority of segments in apmd544/apP44 embryos when expressed under the control of Scer\GAL4ap-md544. The axon guidance defects seen in apmd544/apP44 embryos are not rescued by coexpression of apΔLIM.Scer\UAS and ChiΔLID.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ap-md544. The axon defects seen in Chie5.5 apmd544/apP44 double mutants are completely rescued by ap::ChiΔLID.ΔLIM.Scer\UAS.T:Hsap\MYC expressed under the control of Scer\GAL4ap-md544.
apmd544/apP44 flies show wing defects. This phenotype is suppressed by expression of apScer\UAS.cMa or ap::ChiChAp.Scer\UAS under the control of Scer\GAL4ap-md544.
Expression of SerScer\UAS.cSa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies restores the wing margin and supports growth of the wing. Patches of ectopic wing margin are found in both surfaces of the rescued wings and overgrowth of the wing can be seen. Expression of fngEP3082 under the control of Scer\GAL4ap-md544 rescues the phenotype of apmd544/apUGO35 flies. Patches of ectopic wing margin form and can be associated with outgrowth of the wing. Groups of cells appear to violate the dorsoventral boundary in apmd544/apUGO35 wing discs which are also expressing SerScer\UAS.cSa under the control of Scer\GAL4ap-md544. The mispositioned patches of dorsal or ventral cells form relatively smooth interfaces with surrounding cells. Cells appear to violate the dorsoventral boundary in apmd544/apUGO35 wing discs which are also expressing fngEP3082 under the control of Scer\GAL4ap-md544. Expression of both SerScer\UAS.cSa and fngEP3082 under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in well-developed wings which differentiate ventral features on both surfaces; the wing margin, veins and sense organs on the topologically dorsal surface have exclusively ventral identity.
Lim3Scer\UAS.cOa expressed under the control of Scer\GAL4ap-md544 partially rescues both the wing and embryonic central nervous system phenotype of apmd544/apP44 animals at 18oC and 25oC respectively. The wing, sterility and uncoordinated behaviour phenotypes of apmd544/apP44 flies are rescued by ap::Lim3Scer\UAS.cOa expressed under the control of Scer\GAL4ap-md544 to the same extent as the rescue seen by apScer\UAS.cOa. In contrast, the axon pathfinding phenotype of apmd544/apP44 embryos is only partially rescued by ap::Lim3Scer\UAS.cOa expressed under the control of Scer\GAL4ap-md544.
apmd544/apUGO35 flies rescued by the expression of Hsap\LHX2Scer\UAS.cRa under the control of Scer\GAL4ap-md544 often develop one to two extra bristles in the scutellum. The phenotypes of apmd544/apUGO35 flies are rescued by Hsap\LHX2Scer\UAS.cRa expressed under the control of Scer\GAL4ap-md544. apmd544/apUGO35 flies rescued by the expression of Hsap\LHX2Scer\UAS.cRa under the control of Scer\GAL4ap-md544 often develop one to two extra bristles in the scutellum.
apmd544/Df(2R)nap1 is rescued by apUAS.cOa/Scer\GAL4ap-md544
apmd544 is rescued by Scer\GAL4ap-md544/apUAS.cMa
apmd544/Df(2R)nap1 is rescued by Scer\GAL4ap-md544/apUAS.cBa
apmd544/apUGO35 is rescued by Scer\GAL4ap-md544/apUAS.cRa
apmd544/apP44 is rescued by Scer\GAL4ap-md544/apUAS.cMa
apmd544/apUGO35 is rescued by Scer\GAL4ap-md544/apUAS.cRa
apmd544/apUGO35 is partially rescued by Scer\GAL4ap-md544/apL1.UAS
apmd544/apP44 is partially rescued by apUAS.cOa/Scer\GAL4ap-md544
apmd544/apP44 is not rescued by apΔLIM.UAS/Scer\GAL4ap-md544
apmd544/apP44 is not rescued by apΔLIM.UAS/Scer\GAL4ap-md544
apmd544/apP44 is not rescued by Scer\GAL4ap-md544/apΔHD.UAS
Reduction and fusion of the fourth tarsal segment in apmd544/Df(2R)nap1 animals is rescued by apScer\UAS.cOa.
A single copy of apScer\UAS.cOa expressed under the control of Scer\GAL4ap-md544 almost completely rescues the wing phenotype of apmd544/apP44 flies; the wing blade is normal in size, with a normal margin and vein pattern, but is held at right angles away from the body. The sterility and uncoordinated behaviour phenotypes of apmd544/apP44 flies are also rescued and the central nervous system defects of embryos are rescued. The adult and embryonic phenotypes of apmd544/apP44 animals are not rescued by apΔLIM.Scer\UAS expressed under the control of Scer\GAL4ap-md544. The ribbon-like outgrowth seen in apmd544/apP44 flies is entirely eliminated in flies expressing apΔHD.Scer\UAS under the control of Scer\GAL4ap-md544.