FlyBase Allele Report: Dmel\Mad[UAS.cNa]

General Information

Symbol

Dmel\Mad^UAS.cNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Newfeld

FlyBase ID

FBal0052172

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

Carried in Construct

P{UAS-Mad.N}

Also Known As

UAS-Mad, UAS.Mad

Key Links

Transgenic product class

cDNA

(Newfeld et al., 1996)

wild_type

(Newfeld et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Newfeld et al., 1996)

wild_type

(Newfeld et al., 1996)

Mutagen

in vitro construct

(Newfeld et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-Mad.N}

Cytology

Description

Expression of the complete Mad cDNA is governed by UASt regulatory sequences.

(Newfeld et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mad

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^A9

(Fischer et al., 2012)

visible, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

visible, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

visible, with Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

visible, with Scer\GAL4^vg.PM

(Tsuneizumi et al., 1997)

Phenotype Manifest In

leg | ectopic, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

wing

(Gesualdi and Haerry, 2007)

wing, with Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

wing, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

wing, with Scer\GAL4^T80

(Takaesu et al., 2012)

wing, with Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

wing, with Scer\GAL4^vg.PM

(Tsuneizumi et al., 1997)

wing disc

(Gesualdi and Haerry, 2007)

wing vein

(Gesualdi and Haerry, 2007)

wing vein, with Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

wing vein | ectopic, with Scer\GAL4^A9

(Fischer et al., 2012)

wing vein | ectopic, with Scer\GAL4^ptc-559.1

(Takaesu et al., 2005, Marquez et al., 2001)

wing vein | ectopic, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing vein | ectopic, with Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Detailed Description

Statement

Reference

Overexpression of Mad^Scer\UAS.cNa with Scer\GAL4^A9 leads to a dramatic reduction in wing size, ectopic vein tissue and blistering.

(Fischer et al., 2012)

Expression of Mad^Scer\UAS.cNa in the wing discs under the control of Scer\GAL4^T80 leads to wing vein truncation.

(Takaesu et al., 2012)

Expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^sca-537.4 results in wild-type wings 34% of the time, while the remaining wings have a variety of mild ectopic vein phenotypes.

(Quijano et al., 2011)

Expression of Mad^Scer\UAS.cNa using Scer\GAL4^salm-459.2 has no severe effects on wing growth or patterning.

Expression of Mad^Scer\UAS.cNa using Scer\GAL4^nub-AC-62 causes alterations in the vein pattern and wing size.

(Barrio et al., 2007)

Overexpression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^A9 results in wings of almost normal size that exhibit some ectopic vein tissue.

(Gesualdi and Haerry, 2007)

Expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^vg.int2.1 results in a broader wing and ectopic veins along L2 and L5 and emanating from the posterior crossvein.

(Zeng et al., 2007)

Expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^ptc-559.1 produces excess vein tissue between L3/L4/L5 wing veins.

(Takaesu et al., 2005)

When expression is driven by Scer\GAL4^ptc-559.1, ectopic vein tissue appears between wing veins L3 and L5. The distance between L3 and L5 appears reduced. Wings are 22% smaller than wild type. An ectopic leg develops on the ventral side of the normal limb. The ectopic leg is well formed with several segments and tarsal claws.

(Marquez et al., 2001)

When expression is driven by Scer\GAL4^vg.PM, outgrowth of wing tissue results.

(Tsuneizumi et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has visible phenotype, enhanceable by rl^Sem.S.UAS, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has visible phenotype, enhanceable by rl[+]/rl^Sem

(Zeng et al., 2007)

Suppressed by

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^A9 has abnormal size phenotype, suppressible by fuss^UAS.C, Scer\GAL4^A9

(Fischer et al., 2012)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has visible phenotype, suppressible by nmo^UAS.cUa, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.PM has visible phenotype, suppressible by Dad^UAS.cTa, Scer\GAL4^vg.PM

(Tsuneizumi et al., 1997)

Suppressor of

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^A9 is a suppressor | partially of visible phenotype of Scer\GAL4^A9, Snoo^UAS.cTa

(Takaesu et al., 2006)

NOT Suppressor of

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^elav-C155 is a non-suppressor of abnormal locomotor behavior | heat sensitive phenotype of Hsap\TOR1A^ΔE.UAS, Scer\GAL4^elav-C155

(Koh et al., 2004)

Other

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.UAS has visible phenotype

(Quijano et al., 2011)

Axn^ΔRGS.UAS, Mad^UAS.cNa, Scer\GAL4^sca-537.4 has visible phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has visible phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^A81T.UAS has visible phenotype

(Quijano et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mad^UAS.cNa has wing vein phenotype, enhanceable by Scer\GAL4^A9/babo^Q302D.UAS

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa has wing phenotype, enhanceable by Scer\GAL4^A9/babo^Q302D.UAS

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa has wing disc phenotype, enhanceable by Scer\GAL4^A9/babo^Q302D.UAS

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has wing phenotype, enhanceable by rl[+]/rl^Sem

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has wing vein | ectopic phenotype, enhanceable by rl[+]/rl^Sem

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has wing phenotype, enhanceable by rl^Sem.S.UAS, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has wing vein | ectopic phenotype, enhanceable by rl^Sem.S.UAS, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Suppressed by

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^A9 has wing vein | ectopic phenotype, suppressible | partially by fuss^UAS.C, Scer\GAL4^A9

(Fischer et al., 2012)

Mad^UAS.cNa, Scer\GAL4^nub-AC-62 has wing phenotype, suppressible by Snoo^M67, Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

Mad^UAS.cNa, Scer\GAL4^nub-AC-62 has wing vein phenotype, suppressible by Snoo^M67, Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

Mad^UAS.cNa, Scer\GAL4^nub-AC-62 has wing phenotype, suppressible | partially by Snoo^M67/Snoo^2c3, Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

Mad^UAS.cNa, Scer\GAL4^nub-AC-62 has wing vein phenotype, suppressible | partially by Snoo^M67/Snoo^2c3, Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

Mad^UAS.cNa has wing phenotype, suppressible by Scer\GAL4^A9/myo^UAS.cGa

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa has wing vein phenotype, suppressible by Scer\GAL4^A9/myo^UAS.cGa

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa has wing phenotype, suppressible by Scer\GAL4^A9/Actβ^CM.UAS

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa has wing vein phenotype, suppressible by Scer\GAL4^A9/Actβ^CM.UAS

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has wing phenotype, suppressible by nmo^UAS.cUa, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.int2.1 has wing vein | ectopic phenotype, suppressible by nmo^UAS.cUa, Scer\GAL4^vg.int2.1

(Zeng et al., 2007)

Mad^UAS.cNa, Scer\GAL4^vg.PM has wing phenotype, suppressible by Dad^UAS.cTa, Scer\GAL4^vg.PM

(Tsuneizumi et al., 1997)

Enhancer of

Statement

Reference

Scer\GAL4^A9/Mad^UAS.cNa is an enhancer of wing vein phenotype of babo^Q302D.UAS

(Gesualdi and Haerry, 2007)

Scer\GAL4^A9/Mad^UAS.cNa is an enhancer of wing disc phenotype of babo^Q302D.UAS

(Gesualdi and Haerry, 2007)

Suppressor of

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^salm-459.2 is a suppressor of wing vein phenotype of Scer\GAL4^salm-459.2, Snoo^M67

(Barrio et al., 2007)

Mad^UAS.cNa, Scer\GAL4^nub-AC-62 is a suppressor of wing phenotype of Scer\GAL4^nub-AC-62, Snoo^M67

(Barrio et al., 2007)

Mad^UAS.cNa, Scer\GAL4^nub-AC-62 is a suppressor of wing vein phenotype of Scer\GAL4^nub-AC-62, Snoo^M67

(Barrio et al., 2007)

Mad^UAS.cNa, Scer\GAL4^salm-459.2 is a suppressor of wing phenotype of Scer\GAL4^salm-459.2, Snoo^M67

(Barrio et al., 2007)

Scer\GAL4^A9/Mad^UAS.cNa is a suppressor of wing phenotype of babo^Q302D.UAS

(Gesualdi and Haerry, 2007)

Mad^UAS.cNa, Scer\GAL4^A9 is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Snoo^UAS.cTa

(Takaesu et al., 2006)

Mad^UAS.cNa, Scer\GAL4^A9 is a suppressor | partially of wing vein phenotype of Scer\GAL4^A9, Snoo^UAS.cTa

(Takaesu et al., 2006)

Other

Statement

Reference

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^A81T.UAS has wing vein L3 phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^A81T.UAS has wing sensillum | ectopic phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^A81T.UAS has wing margin bristle | ectopic phenotype

(Quijano et al., 2011)

Axn^ΔRGS.UAS, Mad^UAS.cNa, Scer\GAL4^sca-537.4 has wing margin bristle phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.UAS has wing vein phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.UAS has wing margin bristle phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, sgg^S9A.UAS has wing sensillum phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has wing sensillum phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has wing margin bristle | ectopic phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^sca-537.4, dsh^UAS.cAa has wing vein L3 | distal phenotype

(Quijano et al., 2011)

Mad^UAS.cNa, Scer\GAL4^A9, daw^UAS.cGa has wing phenotype

(Gesualdi and Haerry, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of fuss^Scer\UAS.C in a Mad^Scer\UAS.cNa, Scer\GAL4^A9 background restores not only the size of the wing, but leads to a notable amelioration of the vein-intervein patterning.

(Fischer et al., 2012)

Co-expression of sgg^S9A.Scer\UAS and Mad^Scer\UAS.cNa under the control of Scer\GAL4^sca-537.4 results in wings with no L1 or L3 sensilla, missing margin bristles of all types and occasional vein truncations.

Co-expression of dsh^Scer\UAS.cAa and Mad^Scer\UAS.cNa under the control of Scer\GAL4^sca-537.4 results in wings with no L1 or L3 sensilla, ectopic mechanosensory bristles on the dorsal margin and expansion of the L3 vein distally.

Co-expression of sgg^{A81T.Scer\UAS} and Mad^Scer\UAS.cNa under the control of Scer\GAL4^sca-537.4 results in wings with ectopic sensilla on a slightly thickened L3 vein and ectopic mechanosensory bristles on the dorsal margin.

Flies co-expressing Axn^{ΔRGS.Scer\UAS} and Mad^Scer\UAS.cNa under the control of Scer\GAL4^sca-537.4 have normal wing sensilla with occasional small gaps in the row or stout mechanosensory bristles on the margin.

(Quijano et al., 2011)

Co-expression of Mad^Scer\UAS.cNa suppresses the wing phenotypes caused by Scer\GAL4^salm-459.2-mediated expression of Snoo^M67, giving rise to wings of almost normal size and pattern.

Co-expression of Mad^Scer\UAS.cNa and Snoo^M67 using Scer\GAL4^nub-AC-62 results in wings with a normal pattern of veins.

Scer\GAL4^MD-638-mediated expression of the Snoo^2c3 mutant via the P{GSV1} element associated with Snoo^M67 partially suppresses the wing phenotype caused by Scer\GAL4^MD-638-mediated expression of Mad[Scer\UAS.cNa].

(Barrio et al., 2007)

Co-expression of babo^{Q302D.Scer\UAS} and Mad^Scer\UAS.cNa, both under the control of Scer\GAL4^A9, increases the transformation of intervein into vein tissue. Although the wing discs are larger, the adult wings are smaller due to cell death and transformation of intervein into more tightly associated vein cells.

Co-expression of myoglianin^Scer\UAS.cGa with Mad^Scer\UAS.cNa, both under the control of Scer\GAL4^A9, represses the pattern defects caused by excess Mad^Scer\UAS.cNa.

Co-expression of Actβ^CM.Scer\UAS and Mad^Scer\UAS.cNa, both under the control of Scer\GAL4^A9 dramatically enhances the ectopic wing vein formation seen in Mad^Scer\UAS.cNa mutants alone.

Co-expression of daw^Scer\UAS.cGa and Mad^Scer\UAS.cNa, under the control of Scer\GAL4^A9, decreases the wing size to 88% without affecting ectopic vein formation. Male wing discs are smaller in size in these double mutants.

(Gesualdi and Haerry, 2007)

Co-expression of nmo^Scer\UAS.cUa suppresses the mutant wing phenotype caused by expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^vg.int2.1.

rl^Sem/+ enhances the mutant wing phenotype caused by expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^vg.int2.1.

Co-expression of rl^{Sem.S.Scer\UAS} enhances the mutant wing phenotype caused by expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^vg.int2.1.

(Zeng et al., 2007)

Co-expression of Mad^Scer\UAS.cNa partially rescues the phenotype caused by expression of Snoo^Scer\UAS.cTa under the control of Scer\GAL4^A9; wing size is rescued to wild type and the penetrance of the multiple wing vein defects is reduced to 19%.

(Takaesu et al., 2006)

Dad^Scer\UAS.cTa suppresses the wing outgrowth phenotype of Mad^Scer\UAS.cNa driven by Scer\GAL4^vg.PM, resulting in an almost wild type wing.

(Tsuneizumi et al., 1997)

Xenogenetic Interactions

Statement

Reference

Expression of Mad^Scer\UAS.cNa in flies expressing Hsap\TOR1A^ΔE.Scer\UAS under the control of Scer\GAL4^elav-C155 does not suppress the locomotor defects observed in flies expressing Hsap\TOR1A^ΔE.Scer\UAS alone.

(Koh et al., 2004)

Complementation and Rescue Data

Rescues

Mad^UAS.cNa/Scer\GAL4^elav.PLu rescues Df(2L)C28/Mad¹

(McCabe et al., 2004)

Comments

Expression of Mad^Scer\UAS.cNa under the control of Scer\GAL4^elav.PLu in the neurons rescues neuromuscular junction size in Mad¹/Df(2L)C28 mutants.

(McCabe et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mad^ScerUAS.cNa

(Moser and Campbell, 2005)

Mad^Scer\UAS.cNa

Mad^UAS.cNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Newfeld

(Newfeld et al., 1996)

Secondary FlyBase IDs

References (18)

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