Base pairs 91 to 722 of pgc (containing no T3dh coding sequences) are expressed in an antisense orientation from an Hsp70 promoter.
gonad
primordial germ cell
Causes defects in pole cell migration. Most pole cells fail to colonize the gonad. In resulting adult females, 53% of ovaries are agametic.
Mutants raised at 25oC with two copies of P{hsp70-AS-Pgc}, to eliminate non-specific deleterious effects on oogenesis.
Mutants raised at 25oC with two copies of P{hsp70-AS-Pgc}, to eliminate non-specific deleterious effects on oogenesis.