FlyBase Allele Report: Dmel\dac[UAS.cSa]

General Information

Symbol

Dmel\dac^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Shen

FlyBase ID

FBal0057664

Feature type

allele

Associated gene

Dmel\dac

Associated Insertion(s)

Carried in Construct

P{UAS-dac.S}

Also Known As

UAS-dac

Key Links

Transgenic product class

cDNA

(Shen and Mardon, 1997)

wild_type

(Shen and Mardon, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Shen and Mardon, 1997)

wild_type

(Shen and Mardon, 1997)

Mutagen

in vitro construct

(Chen et al., 1997, Shen and Mardon, 1997)

Progenitor genotype

Carried in construct

P{UAS-dac.S}

Cytology

Description

UASt regulatory sequences drive expression of a full-length dac cDNA.

(Shen and Mardon, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dac

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by

(Moran et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^hs.PB

(Shen and Mardon, 1997)

visible, with Scer\GAL4^7B

(Anderson et al., 2006)

visible, with Scer\GAL4^dpp.blk1

(Dong et al., 2001, Curtiss and Mlodzik, 2000, Chen et al., 1997)

visible, with Scer\GAL4^ey.PH

(Jiao et al., 2001, Curtiss and Mlodzik, 2000)

visible, with Scer\GAL4^GMR.long

(Anderson et al., 2006)

visible, with Scer\GAL4^ptc-559.1

(Rauskolb, 2001)

Phenotype Manifest In

adult head, with Scer\GAL4^dpp.blk1

(Chen et al., 1997)

adult head, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

adult mushroom body, with Scer\GAL4^238Y

(Noveen et al., 2000)

antenna, with Scer\GAL4^dpp.blk1

(Dong et al., 2001, Shen and Mardon, 1997)

antenna, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

anterior-posterior compartment boundary of the leg disc, with Scer\GAL4^dpp.blk1

(Shen and Mardon, 1997)

anterior-posterior compartment boundary of the wing disc, with Scer\GAL4^dpp.blk1

(Shen and Mardon, 1997)

bract | ectopic, with Scer\GAL4^dpp.blk1

(Dong et al., 2001)

eye, with Scer\GAL4^dpp.blk1

(Shen and Mardon, 1997)

eye, with Scer\GAL4^ey.PH

(Jiao et al., 2001, Curtiss and Mlodzik, 2000)

eye | ectopic, with Scer\GAL4^dpp.blk1

(Bui et al., 2000, Curtiss and Mlodzik, 2000, Chen et al., 1997, Shen and Mardon, 1997)

eye | ectopic & adult head, with Scer\GAL4^GMR.long

(Anderson et al., 2006)

eye | ectopic & female genitalia, with Scer\GAL4^7B

(Anderson et al., 2006)

interommatidial bristle, with Scer\GAL4^dpp.blk1

(Shen and Mardon, 1997)

leg, with Scer\GAL4^dpp.blk1

(Chen et al., 1997, Shen and Mardon, 1997)

leg, with Scer\GAL4^ptc-559.1

(Rauskolb, 2001)

leg joint, with Scer\GAL4^ptc-559.1

(Rauskolb, 2001)

ommatidium, with Scer\GAL4^ey.PH

(Curtiss and Mlodzik, 2000)

rhabdomere, with Scer\GAL4^dpp.blk1

(Shen and Mardon, 1997)

wing, with Scer\GAL4^dpp.blk1

(Chen et al., 1997, Shen and Mardon, 1997)

Detailed Description

Statement

Reference

Expression of dac^Scer\UAS.cSa, under the control of Scer\GAL4^GMR.long, results in the formation of a small ectopic eye along the anterior margin of the head cuticle.

Expression of dac^Scer\UAS.cSa, under the control of Scer\GAL4^7B, results in ectopic eye development within the female genitals, but not within male genitals.

(Anderson et al., 2006)

Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 results in the differentiation of medial leg structures (such as bracted bristles) in 100% of antennae.

(Dong et al., 2001)

Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH results pharate adults with a headless phenotype. Four classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae, class III consists of eyeless animals with large parts of the head missing but one or both antennae present and class IV consists of animals in which most of the head and one or both eyes of reduced size are present.

(Jiao et al., 2001)

Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 results in fusion of segments in the leg.

(Rauskolb, 2001)

Expression of dac^Scer\UAS.cSa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult head.

(Bui et al., 2000)

Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 results in the induction of ectopic eyes. Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH results in a variable decrease in the size of dpp^d-blk eyes.

(Curtiss and Mlodzik, 2000)

When expression is driven by Scer\GAL4^238Y minor defects in the mushroom body are evident.

(Noveen et al., 2000)

Expression of dac^Scer\UAS.cSa under the control of Scer\GAL4^30A does not result in the induction of ectopic retinal cells in the wing disc.

(Chen et al., 1999)

Ectopic expression of dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 produces very small ectopic eyes on the anterior surface of the head ventral to the antennae in 56% of flies. Leg and wing morphology are severely disrupted.

(Chen et al., 1997)

In a wild type background Scer\GAL4^dpp.blk1 mediated expression truncates the adult eye in the dorsal-ventral dimension but ommatidial assembly is normal. Expression in the antennal disc and at the anterior-posterior compartment boundary of the leg and wing discs causes increased cell death and truncation of the antenna, leg and wing respectively. 20% of these flies develop ectopic eyes just ventral to the antenna on the anterior surface of the head. Eyes comprise up to 40 to 50 ommatidia that are of abnormal shape (rhabdomeres are unusually small and disorganised) and have irregular bristle arrangement. Ectopic expression also induces retinal development on the thorax just dorsal to the prothoracic leg, only 5% present obvious ommatidial structures. Patches of red pigment are also seen on the leg and wing with no well formed ommatidial structures.

(Shen and Mardon, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, dac^UAS.cSa has visible phenotype, enhanceable by Myc^UAS.cZa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Scer\GAL4^dpp.blk1, dac^UAS.cSa has visible phenotype, enhanceable by eya^E11.UAS/Scer\GAL4^dpp.blk1

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, dac^UAS.cSa has visible phenotype, enhanceable by eya^E7.UAS/Scer\GAL4^dpp.blk1

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, dac^UAS.cSa has visible phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^UAS.cPa

(Chen et al., 1997)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, dac^UAS.cSa has visible phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Enhancer of

Statement

Reference

Scer\GAL4^ey.PH/dac^UAS.cSa is an enhancer of visible | recessive phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

Scer\GAL4^30A/dac^UAS.cSa is an enhancer of visible phenotype of Scer\GAL4^30A, ey^UAS.cHa

(Chen et al., 1999)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has visible phenotype

(Chen et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, dac^UAS.cSa has adult head phenotype, enhanceable by Myc^UAS.cZa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, dac^UAS.cSa has adult head phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Enhancer of

Statement

Reference

Scer\GAL4^ey.PH/dac^UAS.cSa is an enhancer of eye phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

Scer\GAL4^30A/dac^UAS.cSa is an enhancer of wing disc phenotype of Scer\GAL4^30A, ey^UAS.cHa

(Chen et al., 1999)

Scer\GAL4^30A/dac^UAS.cSa is an enhancer of wing phenotype of Scer\GAL4^30A, ey^UAS.cHa

(Chen et al., 1999)

Scer\GAL4^30A/dac^UAS.cSa is an enhancer of haltere phenotype of Scer\GAL4^30A, ey^UAS.cHa

(Chen et al., 1999)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^E7.UAS has eye | ectopic phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^E11.UAS has eye | ectopic phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has wing phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has adult head phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has eye-antennal disc phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has wing disc phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has leg disc phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has dorsal mesothorax phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has femur | dorsal phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has tibia | dorsal phenotype

(Chen et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

eya^E7.Scer\UAS shows a synergy in ectopic eye formation when coexpressed under the control of Scer\GAL4^dpp.blk1 with dac^Scer\UAS.cSa. eya^E11.Scer\UAS shows a synergy in ectopic eye formation when coexpressed under the control of Scer\GAL4^dpp.blk1 with dac^Scer\UAS.cSa.

(Bui et al., 2000)

Enhances the ectopic retinal development phenotype in the wing and haltere discs caused by expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^30A. When ey^Scer\UAS.cHa and dac^Scer\UAS.cSa are coexpressed under the control of Scer\GAL4^30A, large ectopic eyes are seen on the wing and haltere hinges with complete penetrance. Clear ommatidial structures are seen on both the dorsal and ventral side of the wing hinge and the structure of the lens and interommatidial bristles is similar to wild-type.

(Chen et al., 1999)

Coexpression of eya^Scer\UAS.cPa and dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 induces substantial ectopic eyes on the head, legs, wings and dorsal thorax. The cuticle between the normal eye field and the antenna is transformed into retinal cells such that the normal retinal field is expanded. Large patches of pigment are induced on the dorsal side of the femur and tibia of all legs, which are severely truncated. Ommatidial structures are observed in these patches. Red pigment, but no clear ommatidial morphology, is induced on the wing blade. Ectopic eyes are induced bilaterally on the dorsal thorax. All these phenotypes have 100% penetrance. Coexpression of eya^Scer\UAS.cPa and dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 induces ectopic morphogenetic furrow advancement from the ventral side of the eye in late larval eye-antennal discs. Substantial ectopic photoreceptor development is also seen. The axons of these ectopic photoreceptors form a bundle that extends posteriorly into the eye imaginal disc. These axons appear to fuse with the axon tracts sent out by photoreceptors of the normal retinal field and probably exit through the optic stalk and synapse with the larval brain. Ectopic neurons are induced in leg and wing discs. Axons extended by these neurons retract during late larval and pupal development.

(Chen et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^dpp.blk1/dac^UAS.cSa rescues dac¹

(Shen and Mardon, 1997)

Scer\GAL4^dpp.blk1/dac^UAS.cSa rescues dac³

(Shen and Mardon, 1997)

Comments

Scer\GAL4^dpp.blk1 mediated expression of dac^Scer\UAS.cSa rescues eye development in dac³ and dac¹ homozygotes (it fully rescues the morphogenetic furrow initiation defect) but the ommatidial array is disorganised.

(Shen and Mardon, 1997)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

78353

w^*; P{UAS-dac.S}21M5

78352

w^*; P{UAS-dac.S}20M2/CyO

Notes on Origin

Discoverer