FlyBase Allele Report: Dmel\arm[ΔN.UAS.Tag:HA,Tag:Myr(Unk)]

General Information

Symbol

Dmel\arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}

Species

D. melanogaster

Name

FlyBase ID

FBal0057864

Feature type

allele

Associated gene

Dmel\arm

Associated Insertion(s)

Carried in Construct

P{UAS-arm.ΔN.myr.flu}

Also Known As

UAS-ΔArm, UAS-fluΔarm, UAS-Flu-ΔArm, UAS-ArmΔN, UAS-arm*

Key Links

Transgenic product class

inframe_deletion

(Zecca et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Zecca et al., 1996)

Mutagen

in vitro construct

(Hazelett et al., 1998, Brunner et al., 1997, Zecca et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-arm.ΔN.myr.flu}

Cytology

Description

UAS regulatory sequences drive expression of a constitutively active form of arm that lacks the N-terminal part of the protein (amino acids 155-843 are present). The protein is tagged at the N-terminal end with a myristoylation signal (MGNKCCSKRQGTMAGNI) followed by two copies of the Tag:HA epitope. Sequence encoding an inactive nuclear localization signal has been added at the C-terminal end of the protein.

(Zecca et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle | ectopic, with Scer\GAL4^arm.PS, arm¹, arm^{Tag:MYC,Tag:PM(hKRAS)}

(Tolwinski and Wieschaus, 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS

(Tolwinski and Wieschaus, 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS, arm¹, arm^{Tag:MYC,Tag:PM(hKRAS)}

(Tolwinski and Wieschaus, 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS, arm², arm^{Tag:MYC,Tag:PM(hKRAS)}

(Tolwinski and Wieschaus, 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS, arm^{Tag:MYC,Tag:PM(hKRAS)}

(Tolwinski and Wieschaus, 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^da.G32

(Townsley et al., 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^GQ2

(Schupbach and Wieschaus, 1998)

embryonic/larval salivary gland, with Scer\GAL4^Act5C.PP

(Taniue et al., 2010)

eye, with Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

eye | somatic clone, with Scer\GAL4^Act5C.PI

(Baonza and Freeman, 2002, Singh et al., 2002)

eye disc, with Scer\GAL4^Act5C.PP

(Hazelett et al., 1998)

eye disc, with Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

genital disc | somatic clone, with Scer\GAL4^Act5C.PP

(Keisman and Baker, 2001)

nucleus, with Scer\GAL4^Act5C.PP

(Taniue et al., 2010)

ommatidium | somatic clone, with Scer\GAL4^Act5C.PI

(Baonza and Freeman, 2002)

photoreceptor neuron, with Scer\GAL4^Act5C.PP

(Hazelett et al., 1998)

pleural membrane, with Scer\GAL4^GF1

(Schupbach and Wieschaus, 1998)

sternite, with Scer\GAL4^GF1

(Schupbach and Wieschaus, 1998)

ventral denticle belt, with Scer\GAL4^GQ2

(Schupbach and Wieschaus, 1998)

ventral denticle belt, with Scer\GAL4^hs.PB

(Brunner et al., 1997, Zecca et al., 1996)

ventral denticle belt, with Scer\GAL4^{VP16.mat.αTub67C}

(Tolwinski and Wieschaus, 2001)

wing, with Scer\GAL4^71B

(Zeng and Verheyen, 2004)

wing, with Scer\GAL4^HV1

(Schupbach and Wieschaus, 1998)

wing disc | somatic clone, with Scer\GAL4^αTub84B.PZ

(Ryoo et al., 2004)

wing margin bristle | ectopic, with Scer\GAL4^vg.PM

(Bajpai et al., 2004)

wing vein, with Scer\GAL4^71B

(Zeng and Verheyen, 2004)

wing vein, with Scer\GAL4^HV1

(Schupbach and Wieschaus, 1998)

Detailed Description

Statement

Reference

Flip-out clones in the salivary gland expressing arm^{Delta.Scer\UAS.T:Ivir\HA1} have abonormally small nuclei.

(Taniue et al., 2010)

The wings of arm^{Δ.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^vg.PM flies have tufts of ectopic wing margin bristles.

(Bajpai et al., 2004)

Expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PY results in a slight increase in apoptosis in third larval instar eye discs compared to controls. Expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PY results in a rough eye phenotype.

(Kouzmenko et al., 2004)

Somatic clones of arm^{Δ.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^αTub84B.PZ cells in the periphery (proximal regions) of the wing disc show increased BrdU incorporation within the clone compared to wild-type. These clones also distort the morphology of the late third instar wing disc.

(Ryoo et al., 2004)

When arm^{Δ.Scer\UAS.T:Ivir\HA1} is driven by Scer\GAL4^arm.PS a naked cuticle phenotype is seen.

Co-expression of arm^{T:Hsap\MYC,T:Hsap\CAAX} and arm^{Δ.Scer\UAS.T:Ivir\HA1} (driven by Scer\GAL4^arm.PS) in either a wild-type background, or in a arm² background, leads to embryos with naked cuticle. When arm^{T:Hsap\MYC,T:Hsap\CAAX} and arm^{Δ.Scer\UAS.T:Ivir\HA1} are expressed in a arm¹ background however, ectopic denticles are seen.

When arm^{Δ.Scer\UAS.T:Ivir\HA1} is driven in a arm^F1α germ-line clone background, a cuticle pattern with periodic denticle belts and regions of intervening naked cuticle are formed.

(Tolwinski and Wieschaus, 2004)

Overexpression of arm^{Δ.Scer\UAS.T:Ivir\HA1}, under the regulation of Scer\GAL4^da.G32 produces a largely naked ventral cuticle.

(Townsley et al., 2004)

arm^{Δ.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^71B flies have severely abnormal wings.

(Zeng and Verheyen, 2004)

Clones in the wing pouch region of the wing disc expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP during the third larval instar have many fewer cells than control clones induced in parallel. This is primarily due to a slower rate of cell division. Cells in the wing disc expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP cycle aberrantly, compared to internal control cells; the cells have cell cycle phasing characteristic of mature wing disc cells, rather than phasing appropriate for their chronological age. Cell size is normal.

(Johnston and Sanders, 2003)

Clones in the eye expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI show considerable overgrowth and do not differentiate as ommatidia.

(Baonza and Freeman, 2002)

Internal ventral clones in the eye expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI can suppress eye development, whereas clones at the posterior margin do not suppress eye fate.

(Singh et al., 2002)

Clones in the genital disc expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP tend to sort out from the surrounding epithelium.

(Keisman and Baker, 2001)

Expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS results in the complete absence of denticles from the cuticle. Embryos derived from females with arm² homozygous germline clones and carrying Scer\GAL4^arm.PS crossed to males carrying arm^{Δ.Scer\UAS.T:Ivir\HA1} produce embryos with 4 different cuticle phenotypes; small abnormally shaped, denticle covered cuticles (25%, characteristic of arm²), naked cuticles (25%, characteristic of activated arm), wild-type cuticles (25%) and a phenotype where the embryo shows the cell transformations to naked cuticle, but is small and abnormally shaped (25%). This last phenotype seems to be intermediate between the naked and arm² phenotype.

(Tolwinski and Wieschaus, 2001)

Clones expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} in the eye disc under the control of Scer\GAL4^Act5C.PP do not show photoreceptor differentiation within the clone.

(Hazelett et al., 1998)

Embryos expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GQ2 have a naked cuticle phenotype. Adults expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GF1 have abdominal sternites which are enlarged at the expense of the adjacent pleural membrane. Adults expressing arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^HV1 have extra wing vein material between wing veins 2 and 3.

(Schupbach and Wieschaus, 1998)

Causes a 'naked' phenotype, in which the denticles normally located in the anterior of each embryonic segment are partially or completely replaced with naked cuticle, when expressed in a wild-type or ci^Ce-1 background using Scer\GAL4^hs.PB. Does not suppress the lawn of denticles phenotype of pan¹³ flies when expressed using Scer\GAL4^hs.PB, indicating that pan acts downstream of arm.

(Brunner et al., 1997)

Expression under the control of Scer\GAL4^hs.PB causes a larval naked phenotype.

(Zecca et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PY, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has visible phenotype, enhanceable by Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

Scer\GAL4^GMR.PY, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has increased cell death phenotype, enhanceable by Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

Enhancer of

Statement

Reference

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, Scer\GAL4^GMR.PY is an enhancer of visible phenotype of Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, Scer\GAL4^GMR.PY is an enhancer of increased cell death phenotype of Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PY, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has eye disc phenotype, enhanceable by Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

Scer\GAL4^GMR.PY, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has eye phenotype, enhanceable by Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

NOT Enhanced by

Statement

Reference

Scer\GAL4^vg.PM, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has wing margin bristle | ectopic phenotype, non-enhanceable by tws[+]/tws^j11C8

(Bajpai et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^71B, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has wing phenotype, suppressible | partially by Scer\GAL4^71B/nmo^c5-1.UAS

(Zeng and Verheyen, 2004)

Scer\GAL4^71B, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has wing vein phenotype, suppressible | partially by Scer\GAL4^71B/nmo^c5-1.UAS

(Zeng and Verheyen, 2004)

Scer\GAL4^unspecified, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has ventral denticle belt phenotype, suppressible | partially by Axn^A2.UAS, Scer\GAL4^unspecified

(Tolwinski and Wieschaus, 2001)

NOT suppressed by

Statement

Reference

Scer\GAL4^vg.PM, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has wing margin bristle | ectopic phenotype, non-suppressible by tws[+]/tws^j11C8

(Bajpai et al., 2004)

Scer\GAL4^Act5C.PP, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has eye disc phenotype, non-suppressible by tkv^{Q253D.UAS.cNb}/Scer\GAL4^Act5C.PP

(Hazelett et al., 1998)

Scer\GAL4^Act5C.PP, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)} has photoreceptor neuron phenotype, non-suppressible by tkv^{Q253D.UAS.cNb}/Scer\GAL4^Act5C.PP

(Hazelett et al., 1998)

Enhancer of

Statement

Reference

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, Scer\GAL4^GMR.PY is an enhancer of eye disc phenotype of Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, Scer\GAL4^GMR.PY is an enhancer of eye phenotype of Hsap\ESR1^UAS.cKa, Scer\GAL4^GMR.PY

(Kouzmenko et al., 2004)

Suppressor of

Statement

Reference

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^h-1J3 is a suppressor | partially of embryo phenotype of fz2^C1, fz¹⁵

(Chen and Struhl, 1999)

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^h-1J3 is a suppressor | partially of embryonic epidermis phenotype of fz2^C1, fz¹⁵

(Chen and Struhl, 1999)

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^h-1J3 is a suppressor | partially of ventral denticle belt phenotype of fz2^C1, fz¹⁵

(Chen and Struhl, 1999)

NOT Suppressor of

Statement

Reference

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^da.G32 is a non-suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of pygo^S123

(Townsley et al., 2004)

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP is a non-suppressor of photoreceptor neuron | ectopic | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PP

(Hazelett et al., 1998)

Other

Statement

Reference

Scer\GAL4^Act5C.PI, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, tsh^UAS.cGa has eye phenotype

(Singh et al., 2002)

Scer\GAL4^ey.PH, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, tkv^{Q253D.UAS.cNb} has eye disc phenotype

(Lee and Treisman, 2001)

Scer\GAL4^ey.PH, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, tkv^{Q253D.UAS.cNb} has eye disc morphogenetic furrow | ectopic phenotype

(Lee and Treisman, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic wing margin bristles on the wings of arm^{Δ.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^vg.PM animals are unaffected by tws^j11C8/+.

(Bajpai et al., 2004)

Co-expression of Cby^{dsRNA.Scer\UAS.WIZ} in a arm^F1α, arm^{Δ.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS background leads to increased naked cuticle compared to arm^F1α, arm^{Δ.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^arm.PS embryos.

arm^F1α, arm^{Δ.Scer\UAS.T:Ivir\HA1}, Cby^{dsRNA.Scer\UAS.WIZ} (driven by Scer\GAL4^arm.PS) germ-line clones exhibit a naked cuticle phenotype. When Cby^{dsRNA.Scer\UAS.WIZ} is driven by Scer\GAL4^arm.PS in a arm^{Δ.Scer\UAS.T:Ivir\HA1} germ-line clone background, the ectopic denticle phenotype is enhanced.

(Tolwinski and Wieschaus, 2004)

Expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 has no effect on the cuticle phenotype of pygo^S28 or pygo^S123 mutant embryos which lack both maternal and zygotic pygo function.

(Townsley et al., 2004)

The wing phenotype of arm^{Δ.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^71B flies is almost completely suppressed by nmo^{c5-1.Scer\UAS}.

(Zeng and Verheyen, 2004)

Coexpression of both arm^{Δ.Scer\UAS.T:Ivir\HA1} and tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI suppresses eye development in both the dorsal and ventral domains and both in marginal and internal regions of the eye disc. Some of these clones are associated with tissue overgrowth in the adult eye. Coexpression of both arm^{Δ.Scer\UAS.T:Ivir\HA1} and tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in complete suppression of the eye. Coexpression of both arm^{Δ.Scer\UAS.T:Ivir\HA1} and tsh^Scer\UAS.cGa under the control of Scer\GAL4^bi-omb-Gal4 results in eyes which are reduced in both the dorsal and ventral margins in discs and in adults. Coexpression of both wg^Scer\UAS.cAa and tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI results in eye suppression in the dorsal eye disc. Coexpression of both wg^Scer\UAS.cAa and tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in suppression of eye fate at the dorsal and ventral margins of the eye.

(Singh et al., 2002)

Co-expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} and tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^ey.PH in the eye disc often results in ectopic morphogenetic furrow formation from the anterior margin of the disc and a reduction in posterior differentiation.

(Lee and Treisman, 2001)

The naked cuticle phenotype caused by expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^unspecified is partially suppressed by coexpression of Axn^A2.Scer\UAS.

(Tolwinski and Wieschaus, 2001)

The phenotype caused by expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} in clones in the eye disc under the control of Scer\GAL4^Act5C.PP is not rescued if the clones are also co-expressing tkv^{Q253D.Scer\UAS.cNb}. Clones expressing Ras85D^V12.Scer\UAS in the eye disc under the control of Scer\GAL4^Act5C.PP show ectopic photoreceptor formation anterior or posterior to the morphogenetic furrow. This ectopic photoreceptor formation is not prevented by coexpression of arm^{Δ.Scer\UAS.T:Ivir\HA1}.

(Hazelett et al., 1998)

Xenogenetic Interactions

Statement

Reference

Co-expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} and Hsap\ESR1^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PY results in a marked increase in apoptosis in third larval instar eye discs compared to controls. Treatment with estradiol significantly increases the rate of apoptosis in these double mutant flies. Co-expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} and Hsap\ESR1^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PY synergistically enhances the rough eye phenotype seen when either is expressed alone. Treatment of the double mutant flies with estrogen further aggravates the severity of the eye phenotype.

(Kouzmenko et al., 2004)

Complementation and Rescue Data

Fails to rescue

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^arm.PS fails to rescue arm^043A01

(Tolwinski and Wieschaus, 2001)

Comments

The adhesion defect of arm^043A01 is not rescued by expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS.

(Tolwinski and Wieschaus, 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Exists in clones, not reported as being germ-line transmitted.

(Zecca et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Arm*

(Johnston and Sanders, 2003)

arm^{Δ.Scer\UAS.T:Ivir\HA1}

arm^Δ.UAS.T:HA1

arm^{ΔN.Scer\UAS.T:Ivir\HA1,T:Uuuu\Myr-Unk}

arm^{ΔN.Scer\UAS.T:Ivir\HA1,T:Uuuu\Myr5}

arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}

ΔArm

(Tolwinski and Wieschaus, 2001)

Name Synonyms

Secondary FlyBase IDs

References (36)

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