FlyBase Allele Report: Dmel\Gαs[UAS.cCa]

General Information

Symbol

Dmel\Gαs^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Connolly

FlyBase ID

FBal0058938

Feature type

allele

Associated gene

Dmel\Gαs

Associated Insertion(s)

Carried in Construct

P{UAS-Gαs.C}

Also Known As

UAS-Gα_s

Key Links

Transgenic product class

cDNA

(Connolly et al., 1996)

wild_type

(Connolly et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Connolly et al., 1996)

wild_type

(Connolly et al., 1996)

Mutagen

in vitro construct

(Connolly et al., 1996, Wolfgang et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-Gαs.C}

Cytology

Description

A cDNA encoding the short form of Gαs is expressed under the control of UASt regulatory sequences.

(Connolly et al., 1996, Wolfgang et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gαs

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Phenotype Manifest In

labellar taste bristle, with Scer\GAL4^Gr5a.PU

(Ueno et al., 2006)

large labellar taste bristle, with Scer\GAL4^Gr5a.PU

(Ueno et al., 2006)

Detailed Description

Statement

Reference

Expression of G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^P2.4.Pdf significantly reduces the response of the M pacemaker neurons in the brain to Pdf and to Dh31.

Expression of G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^Mai179 significantly reduces the response of the E pacemaker neurons in the brain to Pdf.

(Duvall and Taghert, 2012)

Over 60% of flies expressing G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^P0.5.Pdf in constant darkness are arrhythmic. While the remaining rhythmic flies exhibit normal periods, they are only weakly rhythmic, as shown by a lower rhythm power than control flies.

(Dahdal et al., 2010)

Expression of G-salpha60A^Scer\UAS.cCa under the control of either Scer\GAL4^c309, Scer\GAL4²⁴⁷ (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4^Tab2-201Y (in the γ-lobes) has no effect on spontaneous odor intensity or identity discrimination, nor conditioned odor intensity and identity discrimination.

(Xia and Tully, 2007)

Expression of G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^c205 has no effect on visual pattern memory.

(Liu et al., 2006)

G-sα60A^Scer\UAS.cCa; Scer\GAL4^en-e16E adults have normal wings.

(Kimura et al., 2004)

When expression of G-sα60A^Scer\UAS.cCa is driven in the dopamine and serotonin neurons by Scer\GAL4^Ddc.PL, flies display a reduced responsiveness to an initial dose of cocaine compared to wild-type. Also no sensitization was seen on repeated exposures to cocaine, in contrast to wild-type. Flies expressing G-iα65A^Scer\UAS.cLa under the control of Scer\GAL4^Ddc.PL show significantly decreased locomotor responses to the dopamine D2-like agonist quinpirole compared to controls. Flies expressing G-iα65A^Scer\UAS.cLa under the control of Scer\GAL4^Ddc.PL exhibit no overt behavioural or lethality phenotypes are seen.

(Li et al., 2000)

When expression is driven by Scer\GAL4^238Y, Scer\GAL4^c309, Scer\GAL4^c747 or Scer\GAL4^Tab2-201Y no effect on learning is seen.

(Connolly et al., 1996)

Does not produce a visible phenotype when expressed using Scer\GAL4^30A.

(Wolfgang et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Gαs^UAS.cCa, Scer\GAL4^sNPF-R.2.5 is a suppressor of abnormal feeding behavior | adult stage phenotype of Scer\GAL4^sNPF-R.2.5, sNPF-R^UAS.cLa

(Hong et al., 2012)

Other

Statement

Reference

AdoR^UAS.cDa, Gαs^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype

(Dolezelova et al., 2007)

AdoR^UAS.cDa, Gαs^UAS.cCa, Scer\GAL4^en-e16E has lethal | pharate adult stage phenotype

(Dolezelova et al., 2007)

Phenotype Manifest In

Other

Statement

Reference

AdoR^UAS.cDa, Gαs^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype

(Dolezelova et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Pdfr^Scer\UAS.cMa fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^P2.4.Pdf.

Co-expression of Dh31-R1^Scer\UAS.cDa fully rescues the response of the M pacemaker neurons in the brain to Dh31 to wild-type levels in flies expressing G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^P2.4.Pdf.

(Duvall and Taghert, 2012)

The increase in food consumption compared to controls seen in adults expressing sNPF-R^Scer\UAS.cLa under the control of Scer\GAL4^sNPF-R.2.5 is suppressed by co-expression of Gαs^Scer\UAS.cCa.

(Hong et al., 2012)

Co-expression of Gr21a^Scer\UAS.cKa and Gr63a^Scer\UAS.cKa under the control of Scer\GAL4^Or22a.8010 in the "empty neuron" system ("Δhalo" embryos - Dp(2;2)dpp^d21 + Df(2L)dp-79b - in which Or22a and Or22b are deleted) confers a modest response to CO[[2]] in the ab3A olfactory receptor neuron (ORN). Additional co-expression of G-sα60A^Scer\UAS.cCa does not increase the response of the ab3A ORN to CO[[2]].

(Yao and Carlson, 2010)

Animals co-expressing G-sα60A^Scer\UAS.cCa and AdoR^Scer\UAS.cDa under the control of Scer\GAL4^en-e16E show 20% lethality as pharate adults, whereas expression of either G-sα60A^Scer\UAS.cCa or AdoR^Scer\UAS.cDa alone under the control of Scer\GAL4^en-e16E does not cause lethality. The flies that do emerge show strong wing deformation and melanisation.

(Dolezelova et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Gαs^UAS.cCa/Scer\GAL4^VP16.Orco rescues Gαs^R60

(Yao and Carlson, 2010)

Gαs^UAS.cCa/Scer\GAL4^Gr5a.PU rescues Gαs^R19/G-salpha60A[+]

(Ueno et al., 2006)

Gαs^UAS.cCa/Scer\GAL4^B185 rescues Gαs^B19

(Wolfgang et al., 2004)

Partially rescues

Scer\GAL4^futsch-C380/Gαs^UAS.cCa partially rescues Gαs^B19

(Wolfgang et al., 2004)

Gαs^UAS.cCa/Scer\GAL4^C57 partially rescues Gαs^B19

(Wolfgang et al., 2004)

Gαs^UAS.cCa/Scer\GAL4^Mhc.PW partially rescues Gαs^B19

(Wolfgang et al., 2004)

Gαs^UAS.cCa/Scer\GAL4^elav.PLu partially rescues Gαs^B19

(Wolfgang et al., 2004)

Comments

Expression of G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^{Orco.T:Hsim\VP16} in homozygous G-sα60A^R60 ab3A olfactory receptor neurons rescues the reduced odour response seen in these neurons at near saturating concentrations of either methyl butyrate or butyric acid.

(Yao and Carlson, 2010)

G-sα60A^R19; Scer\GAL4^Tre.PU rescues the reduced trehalose uptake phenotype of G-sα60A^R19/+ flies fed on 10-40mM trehalose.

Stimulation of long labellar taste bristles in G-sα60A^R19/Scer\GAL4^Tre.PU; G-sα60A^Scer\UAS.cCa flies with trehalose induces significantly more biphasic spikes in associated sensory neurons than seen in G-sα60A^R19/+ flies but not significantly more than that seen in wild-type flies.

(Ueno et al., 2006)

When G-sα60A^Scer\UAS.cCa is driven by Scer\GAL4^B185 the synaptic bouton and the late larval lethality phenotypes of G-sα60A^B19 homozygotes. When driven by Scer\GAL4^C380 or Scer\GAL4^elav.PLu the neuromuscular junction phenotypes are rescued to wild-type. When driven by Scer\GAL4^C57 or Scer\GAL4^Mhc.PW in muscles, the defects in bouton formation is rescued, while the branching defects are only partially rescued.