FlyBase Allele Report: Dmel\nej[3]

General Information

Symbol

Dmel\nej³

Species

D. melanogaster

Name

FlyBase ID

FBal0059993

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

Carried in Construct

Also Known As

dCBP³

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Akimaru et al., 1997)

Progenitor genotype

nej^P

(Akimaru et al., 1997)

Cytology

Description

2-3kb deletion near the 5' end of the nej gene.

(Akimaru et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Bodai et al., 2012)

exacerbates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Gavory et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

nej³/+ adults expressing Hsap\HOXA9::Hsap\NUP98^WT.UAS under the control of Scer\GAL4^ey.PH show ectopic wing-like structures in the dorsal-anterior part of the eye.

(Gavory et al., 2021)

Heterozygous nej³ significantly blocks or reduces tolerance to both benzyl alcohol and ethanol.

(Ghezzi et al., 2013)

nej³ does not show any dominant suppressor (Su(var)) or enhancer (E(var)) of variegation activity. Any second site Su(var) mutations on the original chromosome can be separated from nej³ by recombination. Additional copies of nej^+mSa (or nej^T:SV5\V5) fail to rescue the Su(var) phenotype of the original chromosome carrying nej³.

(Smolik, 2009)

Small homozygous clones can be recovered at a low frequency in the wing disc if they are induced in a Minute/+ background.

(Li et al., 2007)

Mutants show developmental arrest soon after the prepupal-pupal transition, with persistent larval salivary glands.

(Yin et al., 2007)

The severe rough eye phenotype caused by expression of nej^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GMR.PF is suppressed if the flies also carry nej³/+. The very mild rough eye phenotype caused by expression of nej^KIX.Scer\UAS under the control of Scer\GAL4^GMR.PF is suppressed if the flies also carry nej³/+. The moderate rough eye phenotype caused by expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry nej³/+. The severe rough eye phenotype caused by expression of nej^{ΔBHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry nej³/+. The mild rough eye phenotype caused by expression of nej^{ΔHQ.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not affected if the flies also carry nej³/+. The severe rough eye phenotype caused by expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^hs.2sev is not affected if the flies also carry nej³/+.

(Kumar et al., 2004)

Homozygous embryos that die at stage 9 often have a characteristic twisted phenotype.

(Ludlam et al., 2002)

Mutant stage 17 embryos do not show significant defects in the location or formation of the tracheal dorsal trunk branch, dorsal branch 10, spiracular branch 10 or the posterior spiracle.

(Takaesu et al., 2002)

Gastric caecae frequently fail to elongate. Formation of the first midgut constriction is often impeded. Dorsal trunk and visceral branches of the developing trachea are essentially normal. Branching defects occur, typically one of two dorsal branches fail to form and ganglionic branches fail to fuse.

(Waltzer and Bienz, 1999)

Only 15% of nej³ hemizygotes die as embryos.

(Florence and McGinnis, 1998)

Mutant embryos look comparatively normal though demonstrate midgut abnormalities reminiscent of sgg mutants. The midgut constriction is present. Epidermis of embryos shows frequent loss of anterior denticles of the denticle belt, similar to the phenotype seen for mild over-activation of wg.

(Waltzer and Bienz, 1998)

Embryos appear twisted at germ band elongation, clockwise or anticlockwise just behind the cephalic furrow, often with the posterior side down. The ventral and cephalic furrows appear normal, but the mesodermally derived internal tissues and the block of ectodermal cells are often missing. Embryos die at stage 9 or 10 during embryogenesis, some embryos survive to hatch. Cuticles of late-lethal embryos exhibit defects. No eggs are produced from germ line clones.

(Akimaru et al., 1997)

Hemizygous embryos generally die at stage 9 or 10, although some survive to hatching. Embryos are twisted from 0-70% egg length at germband elongation.

(Akimaru et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mei-41^RT1, nej[+]/nej³ has abnormal mitotic cell cycle | dominant phenotype, suppressible by nej^+t16

(Smolik and Jones, 2007)

Enhancer of

Statement

Reference

nej[+]/nej³ is an enhancer of decreased size | adult stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

nej[+]/nej³ is an enhancer of visible | adult stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

nej[+]/nej³ is an enhancer of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Bodai et al., 2012)

nej[+]/nej³ is an enhancer of visible | dominant phenotype of so^D

(Kumar et al., 2004)

nej³ is an enhancer of increased cell size | pupal stage phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of abnormal cell shape | pupal stage phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of lethal phenotype of Myb¹/Myb²

(Fung et al., 2003)

nej³ is an enhancer of lethal phenotype of Myb²

(Fung et al., 2003)

nej³ is an enhancer of lethal phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of melanotic mass phenotype phenotype of krz^L8

(Bantignies et al., 2002)

NOT Enhancer of

Statement

Reference

nej³ is a non-enhancer of partially lethal - majority die phenotype of Dfd¹³/Dfd³

(Florence and McGinnis, 1998)

Suppressor of

Statement

Reference

nej[+]/nej³ is a suppressor of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

nej³ is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

nej³ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, sax^{Q263D.UAS.cDa}

(Waltzer and Bienz, 1999)

nej[+]/nej³ is a suppressor of visible | dominant phenotype of ci^D

(Akimaru et al., 1997)

Other

Statement

Reference

mei-41^RT1, nej[+]/nej³ has abnormal mitotic cell cycle | dominant phenotype

(Smolik and Jones, 2007)

nej³, so^D has increased cell death phenotype

(Kumar et al., 2004)

nej[+]/nej³, so^D has increased cell death phenotype

(Kumar et al., 2004)

Myb¹, nej³ has abnormal developmental rate phenotype

(Fung et al., 2003)

Myb², nej³ has abnormal developmental rate phenotype

(Fung et al., 2003)

Myb¹/Myb², nej³ has abnormal developmental rate phenotype

(Fung et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

nej³ has embryonic/larval midgut | embryonic stage phenotype, enhanceable by sgg^M11

(Waltzer and Bienz, 1998)

Suppressed by

Statement

Reference

nej³ has ventral denticle belt | anterior phenotype, suppressible by arm²

(Waltzer and Bienz, 1998)

Enhancer of

Statement

Reference

nej[+]/nej³ is an enhancer of eye phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

nej[+]/nej³ is an enhancer of eye disc phenotype of so^D

(Kumar et al., 2004)

nej[+]/nej³ is an enhancer of eye phenotype of so^D

(Kumar et al., 2004)

nej³ is an enhancer of adult cuticle phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of adult abdominal segment phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of nuclear chromosome phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of wing & cell phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of wing & nucleus phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of wing hair | precursor | increased number phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of wing hair | precursor phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of abdomen phenotype of Myb¹

(Fung et al., 2003)

nej³ is an enhancer of wing vein L4 phenotype of dpp^s1

(Waltzer and Bienz, 1999)

nej³ is an enhancer of wing vein L2 phenotype of dpp^s1

(Waltzer and Bienz, 1999)

nej[+]/nej³ is an enhancer of haltere & macrochaeta | ectopic phenotype of Ubx^M1

(Florence and McGinnis, 1998)

NOT Enhancer of

Statement

Reference

nej[+]/nej³ is a non-enhancer of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

nej³ is a non-enhancer of centrosome phenotype of Myb¹

(Fung et al., 2003)

nej³, Scer\GAL4^30A is a non-enhancer of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Suppressor of

Statement

Reference

nej[+]/nej³ is a suppressor of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

nej³ is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

nej³ is a suppressor of wing margin phenotype of ci^D

(Florence and McGinnis, 1998)

nej[+]/nej³ is a suppressor of wing phenotype of Scer\GAL4^en-e16E, shg^UAS.cSa

(Waltzer and Bienz, 1998)

nej[+]/nej³ is a suppressor of wing phenotype of ci^D

(Akimaru et al., 1997)

nej[+]/nej³ is a suppressor of trichome of the posterior wing margin phenotype of ci^D

(Akimaru et al., 1997)

NOT Suppressor of

Statement

Reference

nej[+]/nej³ is a non-suppressor of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

nej³, Scer\GAL4^30A is a non-suppressor of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Other

Statement

Reference

Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH, nej[+]/nej³ has wing | ectopic phenotype

(Gavory et al., 2021)

Myb¹, nej³ has wing hair | ectopic phenotype

(Fung et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The number of mitotic cells is slightly but significantly higher in hydroxyurea-treated discs from mei-41^RT1/nej³ transheterozygotes compared with heterozygous mei-41^RT1 controls.

Expression of nej^+t16 suppresses the mitosis phenotypes in response to hydroxyurea seen in mei-41^RT1/nej³ transheterozygotes.

(Smolik and Jones, 2007)

nej³/+ ; so^D/+ adults lack eyes and the nondifferentiated field of tissue that is seen in place of the eye in so^D/+ single mutants is replaced by head tissue in the double mutant adults. nej³/+ ; so^D/+ eye imaginal discs are small and undergo increased levels of cell death.

(Kumar et al., 2004)

When nej³ is added to Myb¹ animals at 18^oC a dramatic reduction in viability is seen as well as a significantly delayed emergence of surviving animals. When nej³ is added to Myb¹/Myb² animals an even more marked effect is seen on viability and emergence. When nej³ is added to Myb¹ animals at 18^oC no effect is seen on viability or any delay in development seen. When nej³/+ is added to Myb¹ animals at 18^oC an enhancement of the multiple wing hair phenotype is seen. The abdomen defects are also enhanced. White differentiated cuticle is present between most segments and along the dorsal midline, indicating fusion defects. Defects in wing cell and nucleus size are also enhanced, nuclei and cells are larger and more variable in size. The mitotic index phenotype is also enhanced in these mutants, though no increase in the rate of centrosomal and chromosomal defects is seen.

(Fung et al., 2003)

Double mutants for sgg^M11 and nej³ show a worse gut phenotype than do nej³ single mutants. The embryonic epidmeris phenotype is suppressed in double mutants with arm². Dominantly suppresses the wing margin phenotype of shg^Scer\UAS.cSa, Scer\GAL4^en-e16E.

(Waltzer and Bienz, 1998)

Suppresses a subset of the ci^D wing defects (loss of posterior row wing hairs, abnormal wing shape) in ci^D/+ nej³/+ transheterozygotes.

(Akimaru et al., 1997)

Xenogenetic Interactions

Statement

Reference

One copy of nej³ enhances the reduction in eclosion rate seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155. These flies have a relative eclosion rate of 67.3%.

(Bodai et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

3729

107202

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dCBP³

(Smolik, 2009, Jung and Bonini, 2007, Smolik and Jones, 2007)

nej³

(Alfonso-Gonzalez et al., 2023, Gavory et al., 2021, Nakamura et al., 2019, Ghezzi et al., 2017, Ghezzi et al., 2013, Bodai et al., 2012, Ito et al., 2012, Popkova et al., 2012, Kirilly et al., 2011, Li et al., 2007, Nagraj et al., 2007, Smolik and Jones, 2007, Yin et al., 2007, Ito et al., 2004, Ludlam et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (33)

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