FlyBase Allele Report: Dmel\pk[pk-sple-13]

General Information

Symbol

Dmel\pk^pk-sple-13

Species

D. melanogaster

Name

FlyBase ID

FBal0060943

Feature type

allele

Associated gene

Dmel\pk

Associated Insertion(s)

Carried in Construct

Also Known As

pk^pk-sple13, pk-sple¹³, pk¹³

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Gubb et al., 1999)

Progenitor genotype

Cytology

Description

Lesion present between 40.2 and 66.2kb downstream of zero (zero being 973bp proximal to the pk transcription start site.).

(Gubb et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdomen

(Gubb et al., 1999)

eye

(Gubb et al., 1999)

eye photoreceptor cell | somatic clone

(Strutt et al., 2002)

eye photoreceptor cell | somatic clone (with pk^sple-1)

(Strutt et al., 2002)

leg

(Gubb et al., 1999)

ommatidium

(Strutt and Strutt, 2007, Jenny et al., 2003, Gubb et al., 1999)

ommatidium | somatic clone

(Strutt et al., 2002)

ommatidium | somatic clone (with pk^sple-1)

(Strutt et al., 2002)

photoreceptor cell R4 | precursor | somatic clone

(Jenny et al., 2003)

scutum

(Gubb et al., 1999)

tarsal segment 3

(Gubb et al., 1999)

tarsal segment 3 & joint

(Gubb et al., 1999)

tarsal segment 4

(Gubb et al., 1999)

tarsal segment 4 & joint

(Gubb et al., 1999)

trichome & adult abdomen | somatic clone | cell autonomous

(Lawrence et al., 2004)

triple row of wing sensilla

(Gubb et al., 1999)

wing

(Gubb et al., 1999)

wing | cell autonomous | somatic clone

(Adler et al., 2000)

wing | cell non-autonomous | somatic clone

(Adler et al., 2000)

wing hair

(Gubb et al., 1999)

Detailed Description

Statement

Reference

Overall length of the tracheal dorsal trunk is normal in pk^pk-sple-13/pk^eq stage 16 embryos.

(Chung et al., 2009)

Homozygous adults show defects in ommatidial patterning, with ommatidia having random orientation and chirality.

Homozygous clones in the pupal wing (23 hours after puparium formation) do not affect polarity of trichomes in surrounding wild-type tissue.

(Strutt and Strutt, 2007)

pk^pk-sple-13 somatic clones in the adult abdomen have disturbed polarity - usually a 'whorl' of hairs. This effect is cell autonomous.

(Lawrence et al., 2004)

Mutant animals display a planar cell polarity eye phenotype: misrotations and random chirality with an associated loss of the mirror symmetry line, the equator, at the dorsoventral midline. Chirality defects appear to be associated with a mutant R4 precursor.

(Jenny et al., 2003)

57.8% of ommatidia in pk^pk-sple-13/pk^sple-1 mutant somatic clones in the eye are normal. 0.6% have rotated ommatidia, 41.6% have chirality defects, none are achiral (0% unscorable). 53.0% of ommatidia in homozygous mutant somatic clones in the eye are normal. 4.6% have rotated ommatidia, 41.9% have chirality defects, 0.5% are achiral (0% unscorable).

(Strutt et al., 2002)

85% of homozygous somatic clones in the wing exhibit cell autonomy, though about 15% display a clear domineering non-autonomy. This is not restricted to large or early induced clones.

(Adler et al., 2000)

Causes no embryonic phenotype even when homozygous mutant embryos develop from homozygous mutant mothers. Has a weak polarity phenotype in the wing, notum, abdomen, eye and legs. In the wing, shows a slight effect on triple-row bristle orientation and gently curved hair polarity vectors. In the eye a mixture of chiral forms of ommatidia are seen. Some ommatidia fail to rotate properly, resulting in imperfections in the hexagonal stacking and a slightly rough eye phenotype. Some ommatidia are aligned at 60^o to the equator and some show anterior-posterior axis reversals. The T3 and T4 segments carry medial duplications of the proximal and distal joint structures, with the middle of each segment deleted. This results in alternating reversed-proximal and reversed-distal tarsal joint structures with half the length of a normal segment. T5 is unaffected. Denticle belt morphology and denticle orientation remains wild-type. Wings in pk^pk-sple-13/pk³⁰ flies show a phenotype stronger than pk^pk-sple-13/pk^pk-sple-13 and weaker than pk³⁰/pk³⁰.

(Gubb et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

pk^pk-sple-13 has abnormal cell polarity phenotype, non-suppressible by dsh^hs.PA

(Tree et al., 2002)

Enhancer of

Statement

Reference

pk[+]/pk^pk-sple-13 is an enhancer of abnormal cell polarity phenotype of dco²/dco^5B2.6

(Strutt et al., 2006)

pk^pk-sple-13/pk^pk-sple-13 is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^hh-Gal4, fz^UAS.cSa

(Lawrence et al., 2004)

pk^pk-sple-13 is an enhancer of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, Vang^UAS.cWa

(Jenny et al., 2003)

NOT Enhancer of

Statement

Reference

pk[+]/pk^pk-sple-13 is a non-enhancer of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

pk[+]/pk^pk-sple-13 is a non-enhancer of decreased size | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

pk[+]/pk^pk-sple-13 is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

pk^pk-sple-13/pk^pk-sple-13 is a non-enhancer of abnormal planar polarity phenotype of fz²¹

(Lawrence et al., 2004)

Suppressor of

Statement

Reference

pk[+]/pk^pk-sple-13 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

pk^pk-sple-13 is a suppressor of abnormal cell polarity phenotype of dsh^hs.PA

(Tree et al., 2002)

pk^pk-sple-13 is a suppressor of visible phenotype of N^nd-3

(Cooper and Bray, 1999)

NOT Suppressor of

Statement

Reference

pk[+]/pk^pk-sple-13 is a non-suppressor of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

pk[+]/pk^pk-sple-13 is a non-suppressor of decreased size | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

pk[+]/pk^pk-sple-13 is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of fz²¹

(Strutt and Strutt, 2007)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of Vang^stbm-6

(Strutt and Strutt, 2007)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of abnormal planar polarity phenotype of fz²¹

(Lawrence et al., 2004)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of fz¹⁵

(Lawrence et al., 2004)

pk^pk-sple-13 is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Strutt et al., 1997)

Other

Statement

Reference

Vang^stbm-6, dgo³⁸⁰, pk^pk-sple-13 has abnormal cell polarity | adult stage phenotype

(Strutt et al., 2016)

Vang^stbm-6, dgo³⁸⁰, dsh³, pk^pk-sple-13 has abnormal cell polarity | adult stage phenotype

(Strutt et al., 2016)

CG15283^GD13108, Scer\GAL4^en-e16E, pk[+]/pk^pk-sple-13 has abnormal planar polarity phenotype

(Weber et al., 2012)

CG15283^GD13108, Scer\GAL4^en-e16E, dgo[+]/dgo³⁸⁰, pk[+]/pk^pk-sple-13 has abnormal planar polarity phenotype

(Weber et al., 2012)

Mks1^GD9100, Scer\GAL4^en-e16E, pk[+]/pk^pk-sple-13 has abnormal planar polarity phenotype

(Weber et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

pk^pk-sple-13 has wing hair phenotype, non-suppressible by dsh^hs.PA

(Tree et al., 2002)

Enhancer of

Statement

Reference

pk[+]/pk^pk-sple-13 is an enhancer of wing hair phenotype of dco²/dco^5B2.6

(Strutt et al., 2006)

pk^pk-sple-13/pk^pk-sple-13 is an enhancer of trichome & adult abdomen phenotype of fz^UAS.cSa

(Lawrence et al., 2004)

pk^pk-sple-13 is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, Vang^UAS.cWa

(Jenny et al., 2003)

NOT Enhancer of

Statement

Reference

pk[+]/pk^pk-sple-13 is a non-enhancer of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

pk[+]/pk^pk-sple-13 is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

pk^pk-sple-13/pk^pk-sple-13 is a non-enhancer of trichome & adult abdomen phenotype of fz²¹

(Lawrence et al., 2004)

Suppressor of

Statement

Reference

pk[+]/pk^pk-sple-13 is a suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

pk^pk-sple-13 is a suppressor of wing hair phenotype of dsh^hs.PA

(Tree et al., 2002)

pk^pk-sple-13 is a suppressor of wing vein phenotype of N^nd-3

(Cooper and Bray, 1999)

pk^pk-sple-13 is a suppressor of wing phenotype of N^nd-3

(Cooper and Bray, 1999)

NOT Suppressor of

Statement

Reference

pk[+]/pk^pk-sple-13 is a non-suppressor of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

pk[+]/pk^pk-sple-13 is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

pk^pk-sple-13 is a non-suppressor of ommatidium phenotype of ec^spok

(Montrasio et al., 2007)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of wing hair | somatic clone | cell non-autonomous | pupal stage phenotype of fz²¹

(Strutt and Strutt, 2007)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of wing hair | somatic clone | cell non-autonomous | pupal stage phenotype of Vang^stbm-6

(Strutt and Strutt, 2007)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of trichome & abdomen | cell non-autonomous | somatic clone phenotype of fz¹⁵

(Lawrence et al., 2004)

pk^pk-sple-13/pk^pk-sple-13 is a non-suppressor of trichome & adult abdomen phenotype of fz²¹

(Lawrence et al., 2004)

Other

Statement

Reference

Vang^stbm-6, dgo³⁸⁰, pk^pk-sple-13 has wing hair phenotype

(Strutt et al., 2016)

Vang^stbm-6, dgo³⁸⁰, dsh³, pk^pk-sple-13 has wing hair phenotype

(Strutt et al., 2016)

CG15283^GD13108, Scer\GAL4^en-e16E, pk[+]/pk^pk-sple-13 has wing hair phenotype

(Weber et al., 2012)

CG15283^GD13108, Scer\GAL4^en-e16E, dgo[+]/dgo³⁸⁰, pk[+]/pk^pk-sple-13 has wing hair phenotype

(Weber et al., 2012)

Mks1^GD9100, Scer\GAL4^en-e16E, pk[+]/pk^pk-sple-13 has wing hair phenotype

(Weber et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Vang^stbm-6, dgo³⁸⁰, pk^pk-sple-13 and dsh³, Vang^stbm-6, dgo³⁸⁰, pk^pk-sple-13 triple and quadruple heterozygotes exhibit mild wing trichome orientation defects in proximal regions.

(Strutt et al., 2016)

Flies expressing Mks1^GD9100 under the control of Scer\GAL4^en-e16E in a pk^pk-sple-13/+ genetic background display wild-type wing hair polarity.

Flies expressing CG15283^GD13108 under the control of Scer\GAL4^en-e16E in a pk^pk-sple-13/+ genetic background display wild-type wing hair polarity.

(Weber et al., 2012)

pk^pk-sple-13/+ suppresses the ommatidial over-rotation phenotype which is caused by expression of nmo^Scer\UAS.cUa under the control of Scer\GAL4^hs.2sev.

(Mirkovic et al., 2011)

The ommatidial rotation defects observed in ec^spok hemizygous adult eyes are not suppressed by pk^pk-sple-13/+.

(Montrasio et al., 2007)

pk^pk-sple-13 fz²¹/fz²¹ clones in the pupal wing (32 hours after puparium formation) cause neighbouring cells to point their trichomes towards the clone, as occurs with fz²¹/fz²¹ single mutant clones in the pupal wing.

pk^pk-sple-13 Vang^stbm-6/Vang^stbm-6 clones in the pupal wing (32 hours after puparium formation) cause neighbouring cells to point their trichomes away from the clone, as occurs with Vang^stbm-6/Vang^stbm-6 single mutant clones in the pupal wing.

dgo³⁸⁰ pk^pk-sple-13/pk^pk-sple-13 clones in the pupal wing (32 hours after puparium formation) do not affect polarity of trichomes in surrounding wild-type tissue.

(Strutt and Strutt, 2007)

The planar polarity phenotype in the abdomen of fz²¹/fz²¹ flies is largely unaffected when the whole animal is pk^pk-sple-13/pk^pk-sple-13.

When fz^Scer\UAS.cZa; Scer\GAL4^hh-Gal4 flies are pk^pk-sple-13 homozygous, the zone of repolarisation straddling the anterior-posterior compartment boundaries in adult abdominal segements is enlarged, but otherwise unchanged.

(Lawrence et al., 2004)

The addition of dsh^hs.PA to pk^pk-sple-13 animals has no effect on the pk^pk-sple-13 wing hair polarity phenotype.

(Tree et al., 2002)

Completely suppresses the wing phenotypes (thickened veins and wing-edge loss) of N^nd-3 flies.

(Cooper and Bray, 1999)

Does not alter the eye tissue polarity phenotype produced by fz^Scer\UAS.cAa expressed under the control of Scer\GAL4^hs.2sev.

(Strutt et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pk^pk-sple-13 is rescued by pk^{Act5C.Tag:MYC,Tag:HA,Tag:CS(PSP)}

(Strutt et al., 2013)

pk^pk-sple-13 is rescued by pk^Act5C.pk

(Strutt and Strutt, 2007)

pk^pk-sple-13 is rescued by pk^sev.sple.PS

(Strutt and Strutt, 2007)

pk^pk-sple-13 is rescued by pk^sple.sev

(Jenny et al., 2003)

pk^pk-sple-13 is rescued by pk^{sple.CtoA.sev}

(Jenny et al., 2003)

Partially rescued by

pk^pk-sple-13 is partially rescued by pk^{Act5C.pk.EGFP}

(Strutt et al., 2013)

pk^pk-sple-13 is partially rescued by pk^{ΔCaaX.Act5C.pk.EGFP}

(Strutt et al., 2013)

pk^pk-sple-13 is partially rescued by pk^{Act5C.sple.EGFP}

(Strutt et al., 2013)

pk^pk-sple-13 is partially rescued by pk^{ΔCaaX.Act5C.sple.EGFP}

(Strutt et al., 2013)

Comments

Expression of pk^{Act5C.T:Hsap\MYC,T:Ivir\HA1,T:Zzzz\PSP.CS} fully rescues the wing planar polarity phenotype seen in pk^pk-sple-13 mutant wings.

Expression of pk^{Act5C.pk.T:Avic\GFP-EGFP} fully rescues the wing planar polarity phenotype seen in pk^pk-sple-13 mutant wings.

Expression of pk^{Act5C.pk.T:Avic\GFP-EGFP} partially rescues the eye planar polarity phenotype seen in pk^pk-sple-13 mutant flies. The misrotation is rescued, but the chirality defects are still present.

Expression of pk^{ΔCaaX.Act5C.pk.T:Avic\GFP-EGFP} fails to rescue the wing planar polarity phenotype seen in pk^pk-sple-13 mutant wings.

Expression of pk^{ΔCaaX.Act5C.pk.T:Avic\GFP-EGFP} partially rescues the eye planar polarity phenotype seen in pk^pk-sple-13 mutant flies. The misrotation is rescued, but the chirality defects are still present.

Expression of pk^{Act5C.sple.T:Avic\GFP-EGFP} fully rescues the planar polarity phenotypes seen in pk^pk-sple-13 mutant eyes and legs. The wing planar polarity phenotype is altered but not rescued; the trichomes point proximally and towards vein 3, as is seen in pk¹ mutant flies.

Expression of pk^{ΔCaaX.Act5C.sple.T:Avic\GFP-EGFP} partially rescues the planar polarity phenotypes seen in pk^pk-sple-13 mutant eyes and legs. 9% of ommatidia are still inverted but the misrotation phenotype is completely rescued.

Expression of pk^{ΔCaaX.Act5C.sple.T:Avic\GFP-EGFP} has no effect on the planar polarity phenotype seen in pk^pk-sple-13 mutant wings.

(Strutt et al., 2013)

pk^Act5C.pk can rescue the wing planar polarity defects seen in pk^pk-sple-13 homozygotes. Expression at 16 and 20 hours after puparium formation (APF) results in only negligible polarity defects in the rescued wings, and expression at 24 and 28 hours APF provides partial rescue.

pk^sev.sple.PS rescues the ommatidial polarity defects of pk^pk-sple-13 homozygotes.

Clones expressing fz^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PI in a pk^pk-sple-13/pk^pk-sple-13 background result in non-clonal cells pointing their trichomes away from the clone. This effect extends 8-9 cells from the clone boundary. This effect is still seen if the clones are also mutant for dgo³⁸⁰, but in this case the effect only extends 5-6 cells from the clone boundary.

(Strutt and Strutt, 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

44230

P{neoFRT}42D pk^pk-sple-13 cn¹/CyO

41790

w^*; P{neoFRT}42D pk^pk-sple-13 cn¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

pk-sple¹³

(Strutt et al., 2016, Merkel et al., 2014, Warrington et al., 2013, Peng et al., 2012, Djiane and Mlodzik, 2010, Montrasio et al., 2007, Strutt et al., 2006, Amonlirdviman et al., 2005, Das et al., 2004, Bastock et al., 2003, Strutt et al., 2002, Gubb, 1998, Strutt et al., 1997, Gubb, 1993)

pk¹³

(Collu et al., 2018, Weber et al., 2012, Mirkovic et al., 2011)

pk^null

(Jenny et al., 2003)

pk^pk-sple-13

(Corgiat et al., 2022, Podratz et al., 2013, Shimizu et al., 2011, Strutt and Strutt, 2008, Strutt and Warrington, 2008, Strutt and Strutt, 2007, Lawrence et al., 2004)

pk^pk-sple13

(Strutt et al., 2023, Cho et al., 2022, Cho et al., 2020, Lee et al., 2020, Strutt et al., 2019, Collu et al., 2018, Ressurreição et al., 2018, Warrington et al., 2017, Cho et al., 2015, Strutt et al., 2013, Strutt et al., 2013, Strutt et al., 2011, Pataki et al., 2010, Shimada et al., 2006, Jenny et al., 2005, Lin and Gubb, 2003)

pk^pk-sple

(Strutt et al., 2013)

pk^sple-13

(Chung et al., 2009)

sple¹³

(Cooper and Bray, 1999)

Name Synonyms

Secondary FlyBase IDs

FBal0060882

References (47)

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