FlyBase Allele Report: Dmel\msn[102]

General Information

Symbol

Dmel\msn¹⁰²

Species

D. melanogaster

Name

FlyBase ID

FBal0061022

Feature type

allele

Associated gene

Dmel\msn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Kadrmas et al., 2004)

Mutagen

X ray

(Su et al., 1998, Treisman et al., 1997)

Progenitor genotype

Caused by aberration

In(3)msn¹⁰²

Cytology

Description

Inversion breakpoint within the coding region.

(Su et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

visible

(Treisman et al., 1997)

Phenotype Manifest In

axon, with Scer\GAL4^69B, msn^{P656A,P659A.UAS}

(Su et al., 2000)

egg | somatic clone

(Horne-Badovinac et al., 2012)

embryonic/first instar larval cuticle | dorsal

(Kadrmas et al., 2004, Su et al., 1998)

eye disc, with Scer\GAL4^69B, msn^{P656A,P659A.UAS}

(Su et al., 2000)

eye photoreceptor cell

(Treisman et al., 1997)

eye photoreceptor cell & axon | somatic clone

(Ruan et al., 1999)

eye photoreceptor cell | somatic clone

(Paricio et al., 1999)

lamina | somatic clone

(Su et al., 2000, Ruan et al., 1999)

lamina anlage | somatic clone

(Su et al., 2000)

medulla | somatic clone

(Ruan et al., 1999)

ommatidial precursor cluster | somatic clone

(Paricio et al., 1999)

ommatidium | somatic clone

(Paricio et al., 1999)

photoreceptor & axon, with Scer\GAL4^69B, msn^{P656A,P659A.UAS}

(Su et al., 2000)

photoreceptor & axon | somatic clone

(Su et al., 2000)

photoreceptor cell | precursor & nucleus | somatic clone

(Houalla et al., 2005)

photoreceptor cell R1 & axon (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

photoreceptor cell R1 & axon | somatic clone

(Ruan et al., 1999)

photoreceptor cell R2 & axon (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

photoreceptor cell R2 & axon | somatic clone

(Ruan et al., 1999)

photoreceptor cell R3 & axon (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

photoreceptor cell R3 & axon | somatic clone

(Ruan et al., 1999)

photoreceptor cell R4 & axon (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

photoreceptor cell R4 & axon | somatic clone

(Ruan et al., 1999)

photoreceptor cell R4 | somatic clone

(Paricio et al., 1999)

photoreceptor cell R5 & axon (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

photoreceptor cell R5 & axon | somatic clone

(Ruan et al., 1999)

photoreceptor cell R6 & axon (with msn⁰³³⁴⁹)

(Ruan et al., 2002)

photoreceptor cell R6 & axon | somatic clone

(Ruan et al., 1999)

rhabdomere

(Treisman et al., 1997)

rhabdomere | somatic clone

(Paricio et al., 1999)

Detailed Description

Statement

Reference

Females carrying homozygous follicle cell clones can produce round eggs.

(Horne-Badovinac et al., 2012)

When somatic clones are made in the developing eye, man differentiated R-cell precursor nuclei are localised abnormally at the basal region. This appears to reflect a failure for R-cell precursor nuclei to migrate along the apical-basal axis. About 60% of nuclei exhibit this phenotype. When the size of mutant clone patches exceeds 40 ommatidia, a furrow like structure is often formed within the clone. This was always positioned in parallel to the morphogenetic furrow.

(Houalla et al., 2005)

Approximately 30% of msn¹⁰² homozygous embryos shows a 'dorsal open' phenotype.

(Kadrmas et al., 2004)

Many R1-R6 growth cones fail to stop at the lamina layer in msn⁰³³⁴⁹/msn¹⁰² larvae.

(Ruan et al., 2002)

Somatic clones in the eye leads to pathfinding defects in the photoreceptor axons. While photoreceptor axons derived from clones extend normally into the brain, the R1 to R6 axons fail to elaborate a smooth retinotopic array in the lamina. Many R1 ro R6 axons terminate prematurely giving rise to an uneven lamina neuropil. Surprisingly the growth cones that innervate the medulla appear to expand normally, although their projections are somewhat disorganised. msn^{P656A,P659A.Scer\UAS} when driven by Scer\GAL4^69B in a msn¹⁰² background produces third instar larvae with severe defects in axonal targeting. On leaving the optic stalk axons from photoreceptors form very large axonal bundles and fail to defasciculate in the region of either the lamina or the medulla.

(Su et al., 2000)

In msn¹⁰² somatic clones in the eye ommatidia often have malformed or missing photoreceptors. Those ommatidia that have the normal complement of photoreceptors are often misrotated and display the wrong chiral form or are symmetrical. In mosaic photoreceptor R3/R4 pairs, the msn⁺ photoreceptor has a strong preference for adopting the R3 fate. This can often lead to chirality inversions where the msn⁺ R4 precursor adopts the R3 position displacing the msn¹⁰² R3 precursor. The mosaic analysis also showed that for correct 90^o rotation there is no bias for any particular photoreceptor.

(Paricio et al., 1999)

Homozygous clones in the developing eye result in defects in photoreceptor cell innervation; the lamina appears uneven and abnormal, large bundles of axons are seen in the medulla. R1-R6 photoreceptor cell axons from mutant clones pass over the lamina and terminate abnormally in the medulla.

(Ruan et al., 1999)

Homozygous or msn¹⁰²/msn¹⁷² embryos show defects in dorsal closure.

(Su et al., 1998)

Clones in the eye are externally rough. They contain elongated rhabdomeres and some ommatidia also contain missing or an extra photoreceptor.

(Treisman et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

msn[+]/msn¹⁰² is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

msn¹⁰² is a non-enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

msn[+]/msn¹⁰² is a non-enhancer of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

msn¹⁰² is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

Suppressor of

Statement

Reference

msn[+]/msn¹⁰² is a suppressor | partially of visible phenotype of Rala^35d

(Balakireva et al., 2006)

msn[+]/msn¹⁰² is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

msn[+]/msn¹⁰² is a suppressor of visible phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

msn[+]/msn¹⁰² is a suppressor of abnormal planar polarity phenotype of dsh^hs.sev.B

(Paricio et al., 1999)

NOT Suppressor of

Statement

Reference

msn[+]/msn¹⁰² is a non-suppressor of visible phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

msn[+]/msn¹⁰² is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

msn¹⁰² is a non-suppressor of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

msn[+]/msn¹⁰² is a non-suppressor of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

msn¹⁰² is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by stck[+]/stck¹⁸

(Kadrmas et al., 2004)

msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by ics^BG02577/ics^BG02577

(Kadrmas et al., 2004)

msn¹⁷²/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by tkv^{Q253D.UAS.cNb}/Scer\GAL4^69B

(Su et al., 1998)

msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by Jra^Asp.hs.sev

(Su et al., 1998)

Enhancer of

Statement

Reference

msn¹⁰² is an enhancer of photoreceptor & axon phenotype of dock⁰⁴⁷²³

(Su et al., 2000)

msn[+]/msn¹⁰² is an enhancer of embryonic/first instar larval cuticle | dorsal phenotype of bsk¹

(Su et al., 1998)

NOT Enhancer of

Statement

Reference

msn[+]/msn¹⁰² is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

msn¹⁰² is a non-enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

msn[+]/msn¹⁰² is a non-enhancer of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

msn¹⁰² is a non-enhancer of photoreceptor cell R1 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-enhancer of photoreceptor cell R2 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-enhancer of photoreceptor cell R3 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-enhancer of photoreceptor cell R4 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-enhancer of photoreceptor cell R5 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-enhancer of photoreceptor cell R6 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

Suppressor of

Statement

Reference

msn[+]/msn¹⁰² is a suppressor | partially of microchaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn[+]/msn¹⁰² is a suppressor of macrochaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn[+]/msn¹⁰² is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

msn[+]/msn¹⁰² is a suppressor of eye phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

msn[+]/msn¹⁰² is a suppressor of ommatidium phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

msn[+]/msn¹⁰² is a suppressor of ommatidium phenotype of dsh^hs.sev.B

(Paricio et al., 1999)

NOT Suppressor of

Statement

Reference

msn[+]/msn¹⁰² is a non-suppressor of wing phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

msn[+]/msn¹⁰² is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

msn[+]/msn¹⁰² is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Fiehler and Wolff, 2008)

msn¹⁰² is a non-suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

msn[+]/msn¹⁰² is a non-suppressor of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

msn¹⁰² is a non-suppressor of photoreceptor cell R1 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-suppressor of photoreceptor cell R2 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-suppressor of photoreceptor cell R3 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-suppressor of photoreceptor cell R4 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-suppressor of photoreceptor cell R5 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

msn¹⁰² is a non-suppressor of photoreceptor cell R6 & axon phenotype of Scer\GAL4^GMR.PF, bif^UAS.cRa

(Ruan et al., 2002)

Other

Statement

Reference

bsk², msn[+]/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

bsk^flp147E, msn[+]/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

hep¹, msn[+]/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

hep^r75, msn[+]/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

bsk¹/bsk[+], msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

bsk²/bsk[+], msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

bsk^flp147E/bsk[+], msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

hep[+]/hep^r75, msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

hep[+]/hep¹, msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

msn¹⁰² does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; msn¹⁰² clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36^+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, the proportion of Df(1)su(s)R194/+ cells in the larval eye disc is not increased by the presence of msn¹⁰².

(Tyler et al., 2007)

Introduction of a single copy of stck¹⁸ into the homozygous msn¹⁰² background reduces the incidence of a 'dorsal open' phenotype from approximately 30% to approximately 20%.

Homozygosity for ics^BG02577 suppresses the msn¹⁰² 'dorsal open' phenotype, reducing its incidence from approximately 30% to approximately 2%.

(Kadrmas et al., 2004)

The photoreceptor projection phenotype caused by expression of bif^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF is not modified by msn¹⁰².

(Ruan et al., 2002)

msn¹⁰², suppresses the ommatidial polarity defects seen in dsh^hs.sev.B flies grown at 29^oC. About 85% of ommatidia are in their correct polarity compared to 55% in dsh^hs.sev.B alone.

(Paricio et al., 1999)

msn¹⁰²/+ in double heterozygous combination with either bsk¹/+, bsk²/+, bsk^flp147E/+, hep¹/+ or hep^r75/+ produces embryos with a dorsal open phenotype. The dorsal open phenotype of msn¹⁰²/msn¹⁷² embryos is partially rescued by Jra^Asp.hs.sev expressed using heat shock or by tkv^{Q253D.Scer\UAS.cNb} expressed under the control of Scer\GAL4^69B.

(Su et al., 1998)

Xenogenetic Interactions

Statement

Reference

msn¹⁰²/+ does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

(Marchal et al., 2012)

Complementation and Rescue Data

Rescued by

msn¹⁰² is rescued by msn^UAS.cSa/Scer\GAL4^69B

(Su et al., 2000)

msn¹⁰² is rescued by msn^UAS.cSa/Scer\GAL4^69B

(Su et al., 1998)

Partially rescued by

msn¹⁰² is partially rescued by msn^{P656A,P659A.UAS}/Scer\GAL4^69B

(Su et al., 2000)

msn¹⁰² is partially rescued by msn^-CT.UAS/Scer\GAL4^69B

(Su et al., 2000)

msn¹⁰² is partially rescued by msn^{Δ332-667.UAS}/Scer\GAL4^69B

(Su et al., 2000)

Not rescued by

msn¹⁰² is not rescued by Scer\GAL4^69B/msn^KD.UAS

(Su et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5945

w^*; msn¹⁰² P{neoFRT}80B/TM6B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

msn¹⁰²

(Deichsel et al., 2024, Kiely and Gilbert-Ross, 2021, Isabella and Horne-Badovinac, 2016, Muñoz-Soriano et al., 2016, Horne-Badovinac et al., 2012, Marchal et al., 2012, Djiane and Mlodzik, 2010, Fiehler and Wolff, 2008, Buff et al., 2007, Muñoz-Descalzo et al., 2007, Tyler et al., 2007, Balakireva et al., 2006, Delaney et al., 2006, Polaski et al., 2006, Kadrmas et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (25)

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