FlyBase Allele Report: Dmel\Pen[D14]

General Information

Symbol

Dmel\Pen^D14

Species

D. melanogaster

Name

FlyBase ID

FBal0061691

Feature type

allele

Associated gene

Dmel\Pen

Associated Insertion(s)

Carried in Construct

Also Known As

imp-α2^D14

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Torok et al., 1995)

Progenitor genotype

P{lacW}Pen^k14401a

(Torok et al., 1995)

Cytology

Description

Imprecise excision of the P{lacW} element deleting 1.7kb of the Pen transcription unit.

(Torok et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | male

(Mason et al., 2002)

decreased size | adult stage

(Serway et al., 2020)

decreased size | adult stage (with Pen^mbmB)

(Serway et al., 2020)

female sterile

(Serway et al., 2020, Gorjanacz et al., 2002)

female sterile (with Pen^mbmB)

(Serway et al., 2020)

female sterile | recessive

(Mason et al., 2002)

fertile (with Pen^k06324)

(Cook, 2014.7.8)

lethal - all die during embryonic stage | maternal effect

(Gorjanacz et al., 2006)

majority die during embryonic stage | maternal effect, with Pen^-UTR.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

male semi-sterile

(Gorjanacz et al., 2002)

male sterile | recessive

(Mason et al., 2002)

partially lethal - majority die | maternal effect, with Pen^-UTR.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

partially lethal - majority live | maternal effect

(Virágh et al., 2012)

partially lethal - majority live | maternal effect, with Pen^GD10665, Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

partially lethal - majority live | maternal effect, with Pen^UASp.cGa, Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

some die during embryonic stage | maternal effect

(Virágh et al., 2012)

some die during embryonic stage | maternal effect, with Pen^GD10665, Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

some die during embryonic stage | maternal effect, with Pen^UASp.cGa, Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

viable (with Pen^k06324)

(Cook, 2014.7.8)

Phenotype Manifest In

Detailed Description

Statement

Reference

Pen^D14/Pen^D14 or Pen^D14/Pen^mbmB adults exhibit a reduction in mushroom body volume.

(Serway et al., 2020)

The neuromuscular junction appears grossly normal in homozygous third instar larvae; the number of boutons, total synaptic area, average muscle surface area and the bouton number/muscle surface area not altered compared to wild-type controls.

Homozygous third instar larvae have axon commissure defects, with apparent discontinuities in the commissure (all larvae have at least one discontinuity and 89% have 2 or more as assayed by Fas3 immunoreactivity).

Mutant larvae have defects in the patterning of the body-wall muscles. Most frequently, muscles 6 and 7 appear crossed (instead of running parallel as in wild type), appearing to insert improperly at the insertion site of the other. Muscle 5 shows two types of defect; it is either abnormally thin or absent altogether. Branched muscle fibres are also seen at several positions.

(Mosca and Schwarz, 2010)

Homozygous females lay on average only 1.2 eggs/day. None of the eggs hatch.

(Gorjanacz et al., 2006)

Homozygous mothers re unable to lay eggs, except in rare cases. Egg development in mutant mothers in retarded and the lower extremity of each ovary is occluded by shorter than normal eggs displaying deformed egg appendages. The rows of blocked egg chambers remain in the ovaries and later degenerate. Few mature eggs are deposited. They are all shorter and smaller in size than normal, and their dorsal appendages are reduced in length, flattened and frequently fused. Mutant egg chambers exhibit a dumpless phenotype. Homozygous mutant clones in the follicle cells exhibit a wild-type phenotype, but mutant germ-line clones give rise to a dumpless phenotype. Although ring canals in mutant egg chambers reach a diameter similar to wild-type rings they are partially occluded by an apparently loose masses of actin filaments often organised in concentric rings with a small opening in the central area.

(Gorjanacz et al., 2002)

In rare cases, homozygous males produce a few offspring when mated with wild-type females. Homozygous male and female adults appear morphologically wild type. Homozygous testes do contain large numbers of elongated sperm bundles, but squashed testes release few, if any, motile sperm and no sperm are observed in the seminal receptacle or spermathecum of wild-type females mated to homozygous males. A large number of spermatids fail to individualise in homozygous testes and remain syncytial. Normal appearing individualisation complexes are seen. The organisation of the sperm axonemes and structure of the minor and major mitochondrial derivatives appear normal. Eggs laid by homozygous females are smaller than normal and appear deflated. Dorsal appendages are missing, fused together or otherwise severely deformed. The chorions are thinner than normal.

(Mason et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Fs(2)Ket^RE34

(Virágh et al., 2012)

Fs(2)Ket^RX13, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^NLSB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^RX13, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Pen^SNLSB-.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^NLSB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Pen^SNLSB-.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

Fs(2)Ket^e02657, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^NLSB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^e02657, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Pen^SNLSB-.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

Fs(2)Ket^e03750, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^NLSB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^e03750, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Pen^SNLSB-.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

Fs(2)Ket^RP13, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^NLSB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^RP13, Pen^D14 has increased mortality during development | maternal effect phenotype, enhanceable by Pen^SNLSB-.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Virágh et al., 2012)

NOT Enhanced by

Statement

Reference

Fs(2)Ket^RX13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^RX13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Fs(2)Ket^c02473

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Fs(2)Ket^e02657

(Virágh et al., 2012)

Fs(2)Ket^e02657, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^e02657, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Fs(2)Ket^e03750

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Fs(2)Ket^RP13

(Virágh et al., 2012)

Fs(2)Ket^e03750, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^e03750, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Fs(2)Ket^RP13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^RP13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-enhanceable by Fs(2)Ket^RX13

(Virágh et al., 2012)

Suppressed by

Statement

Reference

Fs(2)Ket^RE34, Pen^D14 has increased mortality during development | maternal effect phenotype, suppressible | partially by Pen^+t6

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has increased mortality during development | maternal effect phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/Pen^UASp.cGa

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has increased mortality during development | maternal effect phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/Pen^-UTR.UASp

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has increased mortality during development | maternal effect phenotype, suppressible | partially by Fs(2)Ket^+t13.8

(Virágh et al., 2012)

Pen^D14 has male sterile | recessive phenotype, suppressible by Scer\GAL4^hs.PB/Kap-α3^UAS.cMa

(Mason et al., 2002)

Pen^D14 has male sterile | recessive phenotype, suppressible by Kap-α1^UAS.cMa/Scer\GAL4^hs.PB

(Mason et al., 2002)

NOT suppressed by

Statement

Reference

Fs(2)Ket^RE34, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UASp.cMa

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Kap-α3^-UTR.UASp

(Virágh et al., 2012)

Fs(2)Ket^e02657, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^e02657, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Fs(2)Ket^e03750, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^e03750, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Fs(2)Ket^RX13

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Fs(2)Ket^e02657

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Fs(2)Ket^e03750

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Fs(2)Ket^c02473

(Virágh et al., 2012)

Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Fs(2)Ket^RP13

(Virágh et al., 2012)

Fs(2)Ket^RP13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^RP13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Fs(2)Ket^RX13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^RX13, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14 has increased mortality during development | maternal effect phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Virágh et al., 2012)

Pen^D14 has female sterile | recessive phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UAS.cMa

(Mason et al., 2002)

Pen^D14 has female sterile | recessive phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Kap-α3^UAS.cMa

(Mason et al., 2002)

Enhancer of

Statement

Reference

Pen^D14 is an enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RE34

(Virágh et al., 2012)

NOT Enhancer of

Statement

Reference

Pen^D14 is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^c02473

(Virágh et al., 2012)

Pen^D14 is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RP13

(Virágh et al., 2012)

Pen^D14 is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e02657

(Virágh et al., 2012)

Pen^D14 is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e03750

(Virágh et al., 2012)

Pen^D14 is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RX13

(Virágh et al., 2012)

NOT Suppressor of

Statement

Reference

Pen^D14 is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e02657

(Virágh et al., 2012)

Pen^D14 is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e03750

(Virágh et al., 2012)

Pen^D14 is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RX13

(Virágh et al., 2012)

Pen^D14 is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RP13

(Virágh et al., 2012)

Pen^D14 is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^c02473

(Virágh et al., 2012)

Other

Statement

Reference

Fs(2)Ket^RE34, Pen^D14 has abnormal mitotic cell cycle | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has abnormal mitotic cell cycle | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has abnormal developmental rate | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has abnormal developmental rate | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Df(3L)kto2/+, Pen^D14 has male semi-sterile | dominant phenotype

(Mason et al., 2002)

Df(3R)by416/+, Pen^D14 has male semi-sterile | dominant phenotype

(Mason et al., 2002)

Fs(2)Ket^RE34, Pen^D14 has female sterile phenotype

(Erdelyi et al., 1997)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Pen^D14 has nurse cell ring canal phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Pen^D14 has nurse cell ring canal phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UASp.cMa

(Mason et al., 2003)

Other

Statement

Reference

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has mitotic spindle | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has aster | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has mitotic spindle microtubule | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has nuclear envelope | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^c02473, Pen^D14, Pen^NLSB-.UASp, Scer\GAL4^{VP16.nanos.UTR} has spindle pole centrosome | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has mitotic spindle | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has aster | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has mitotic spindle microtubule | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has nuclear envelope | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Fs(2)Ket^RE34, Pen^D14 has spindle pole centrosome | maternal effect | embryonic stage phenotype

(Virágh et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Kap-α1^Scer\UAS.cMa or Kap-α3^Scer\UAS.cMa under the control of Scer\GAL4^hs.PB using heat shock partially rescues the sterility of Pen^D14 males.

(Mason et al., 2002)

Fs(2)Ket^RE34 Pen^D14 double heterozygotes deposit as many eggs as wild type, they look normal but embryogenesis never commences.

(Erdelyi et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Pen^mbmB

(Serway et al., 2020)

Rescued by

Pen^D14 is rescued by Scer\GAL4^how-24B/Pen^UAS.cMa

(Mosca and Schwarz, 2010)

Pen^D14 is rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^UASp.cMa

(Mason et al., 2002)

Pen^D14 is rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^S56A.UASp

(Mason et al., 2002)

Partially rescued by

Pen^D14 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^ΔIBB.UASp

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^S37A.UASp

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^{S56A.UASp.cGa}

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Pen^S98A.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^3xSA.UASp

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Pen^SNLSB-.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^DIM-.UASp

(Gorjanacz et al., 2006)

Pen^D14 is partially rescued by Pen^UAS.cMa/Scer\GAL4^hs.PB

(Mason et al., 2002)

Not rescued by

Pen^D14 is not rescued by Scer\GAL4^elav.PLu/Pen^UAS.cMa

(Mosca and Schwarz, 2010)

Pen^D14 is not rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^NLSB-.UASp

(Gorjanacz et al., 2006)

Pen^D14 is not rescued by Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp

(Gorjanacz et al., 2006)

Pen^D14 is not rescued by Scer\GAL4^T155/Pen^UASp.cGa

(Gorjanacz et al., 2002)

Comments

Expression of Pen^Scer\UAS.cMa under the control of Scer\GAL4^how-24B largely rescues the somatic muscle patterning defects seen in Pen^D14 larvae.

Expression of Pen^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu does not rescue the somatic muscle patterning defects seen in Pen^D14 larvae.

(Mosca and Schwarz, 2010)

Expression of Pen^{Scer\UAS.P\T.cGa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 13.6 eggs/day and 88.2% of the eggs hatch.

Expression of Pen^{ΔIBB.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 12.0 eggs/day. However, 86% of the eggs have abnormally fused dorsal appendages, approximately 10% are shorter than normal and none of the eggs hatch. 27% of ring canals in the ovary are fully occluded by actin.

Expression of Pen^{S37A.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 12.2 eggs/day. However, only 8.4% of the eggs hatch.

Expression of Pen^{S56A.Scer\UAS.P\T.cGa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 12.4 eggs/day. Meiosis occurs in most eggs, with the formation of polar bodies. However, mitosis in the embryos is considerably delayed and asynchronous. Only 3.4% of the eggs hatch.

Expression of Pen^{S98A.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 13.0 eggs/day. 31.2% of the eggs hatch.

Expression of Pen^{3xSA.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 10.9 eggs/day. Only 9.1% of the eggs hatch.

Expression of Pen^{SNLSB-.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 12.1 eggs/day. However, none of the eggs hatch.

Expression of Pen^{NLSB-.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the low rate of egg laying in Pen^D14 females. None of the eggs that are laid hatch. Ring canals in the ovary are occluded by actin.

Expression of Pen^{DIM-.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in Pen^D14 females restores egg laying to an average of 13.1 eggs/day. However, none of the eggs hatch.

Expression of Pen^{CASB-.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the low rate of egg laying in Pen^D14 females. None of the eggs that are laid hatch. Ring canals in the ovary are occluded by actin.

(Gorjanacz et al., 2006)

Expression of Pen^Scer\UAS.cMa under the control of Scer\GAL4^hs.PB using heat shock partially rescues the sterility of Pen^D14 males. Expression of Pen^{Scer\UAS.P\T.cMa} or Pen^{S56A.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the sterility of Pen^D14 females. Expression of Kap-α1^{Scer\UAS.P\T.cMa} or Kap-α3^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the sterility of Pen^D14 females. Pen^D14/+ ; Df(3L)kto2/+ females are fertile. 90% of Pen^D14/+ ; Df(3R)by416/+ females are fertile.

(Mason et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25394

w^*; Pen^D14/CyO; P{GAL4-nanos.NGT}9/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

D14

(Torok et al., 1995)

Pen^D14

(Cook, 2014.7.8)

imp-alpha2D14

(Virágh et al., 2007)

imp-α2^D14

(Serway et al., 2020, Virágh et al., 2012, Mosca and Schwarz, 2010, Gorjanacz et al., 2006, Gorjanacz et al., 2002)

importin α2^D14

(Mason et al., 2002)

oho31^D14

(Torok et al., 1995)

α2^D14

(Mason et al., 2003)

Name Synonyms

importin-α2D14

(Hogarth et al., 2005)

Secondary FlyBase IDs

References (13)

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