Internal deletion of a 588bp PstI fragment (between 837 and 1425, amino acids 192-389).
Expression of HC2.hs using heat shock only induces ectopic wing veins when it is overexpressed between day 5 and 6 after egg deposition. The phenocritical period is restricted to the prepupal and early pupal stage, starting approximately at the larval-prepupal transition. Bifurcation of wing vein L2 is sometimes seen. Overexpression of HC2.hs during the mid- to late-third instar does not result in ectopic vein formation. Co-expression of Hhs.PM and HC2.hs results in a synergistic phenotype with strong induction of ectopic wing veins.
Heat induced expression causes no or negligible bristle duplications or socket to shaft transformations. Expression does cause a specific net-like wing venation is observed on the wings.
HC2.hs has visible | heat sensitive phenotype, enhanceable by CycB3L6540/CycB3[+]
HC2.hs has visible | heat sensitive phenotype, enhanceable by heixk11403/heix[+]
HC2.hs has visible | heat sensitive phenotype, enhanceable by Df(3R)Delta-FX3/+
HC2.hs has visible | heat sensitive phenotype, suppressible by Df(1)N-54l9
HC2.hs has visible | heat sensitive phenotype, suppressible by Df(1)sc-B57/+
HC2.hs, net1 has partially lethal - majority die | heat sensitive phenotype
HC2.hs has wing vein | ectopic | heat sensitive phenotype, enhanceable by CycB3L6540/CycB3[+]
HC2.hs has wing | heat sensitive phenotype, enhanceable by CycB3L6540/CycB3[+]
HC2.hs has wing vein | ectopic | heat sensitive phenotype, enhanceable by heixk11403/heix[+]
HC2.hs has wing | heat sensitive phenotype, enhanceable by heixk11403/heix[+]
HC2.hs has wing vein | ectopic | heat sensitive phenotype, enhanceable by Df(3R)Delta-FX3/+
HC2.hs has wing | heat sensitive phenotype, enhanceable by Df(3R)Delta-FX3/+
HC2.hs has wing vein | ectopic | heat sensitive phenotype, suppressible by Df(1)N-54l9
HC2.hs has wing vein | ectopic | heat sensitive phenotype, suppressible by Df(1)sc-B57/+
HC2.hs has wing | heat sensitive phenotype, suppressible by Df(1)N-54l9
HC2.hs has wing | heat sensitive phenotype, suppressible by Df(1)sc-B57/+
Carried in plasmid "C2" and expressed in S2 cell culture to test for proper protein expression.