FlyBase Allele Report: Dmel\eya[UAS.cBa]

General Information

Symbol

Dmel\eya^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Bonini

FlyBase ID

FBal0083045

Feature type

allele

Associated gene

Dmel\eya

Associated Insertion(s)

Carried in Construct

P{UAS-eya.B.I}

Also Known As

UAS-eya

Key Links

Transgenic product class

cDNA

(Bonini et al., 1997)

wild_type

(Bonini et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bonini et al., 1997)

wild_type

(Bonini et al., 1997)

Mutagen

in vitro construct

(Bonini et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-eya.B.I}

Cytology

Description

A full-length eya type I cDNA is expressed under the control of UASt regulatory sequences.

(Bonini et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

eya

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antennal segment, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

antennal segment & eye | ectopic, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

egg chamber, with Scer\GAL4^hs.PB

(Bai and Montell, 2002)

egg chamber & basement membrane | apical, with Scer\GAL4^upd1.PU

(Medioni and Noselli, 2005)

eye, with Scer\GAL4^ey.PH

(Curtiss and Mlodzik, 2000)

eye | ectopic, with Scer\GAL4^dpp.blk1

(Bui et al., 2000, Curtiss and Mlodzik, 2000, Seimiya and Gehring, 2000, Bonini et al., 1997)

follicle cell, with Scer\GAL4^hs.PB

(Bai and Montell, 2002)

leg, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

leg & ommatidium | ectopic, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

ommatidium, with Scer\GAL4^ey.PH

(Curtiss and Mlodzik, 2000)

ommatidium | ectopic, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

ovary, with Scer\GAL4^GMR21A11

(Abrieux et al., 2020)

ovary, with Scer\GAL4^{GMR.Switch.PR}

(Abrieux et al., 2020)

ovary, with Scer\GAL4^{Ilp2.Switch.PK}

(Abrieux et al., 2020)

polar follicle cell, with Scer\GAL4^upd1.PU

(Medioni and Noselli, 2005, Bai and Montell, 2002)

stalk follicle cell, with Scer\GAL4^hs.PB

(Bai and Montell, 2002)

wing, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

wing & ommatidium | ectopic, with Scer\GAL4^dpp.blk1

(Bonini et al., 1997)

Detailed Description

Statement

Reference

Expression of eya^Scer\UAS.cBa driven by Scer\GAL4^GMR.PU does not induce any discernible eye phenotypes.

(Wang et al., 2016)

Scer\GAL4^GMR.PU-mediated expression of eya^Scer\UAS.cBa does not affect eye development.

(Anderson et al., 2014)

Overexpression of eya^Scer\UAS.cBa using Scer\GAL4^upd blocks anterior polar cell differentiation in egg chambers. As a consequence, the apical cap, which is formed from basement membrane components, that covers anterior polar cells in wild-type egg chambers is not present.

(Medioni and Noselli, 2005)

Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^hs.PB results in compound egg chambers which are not separated by interfollicular stalks and which are not always completely enveloped by the somatic epithelial layer. No germ cells are connected to each other by more than four ring canals indicating that there has not been an extra round of cell division. Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^upd results in one-third of egg chambers lacking polar cells at one end.

(Bai and Montell, 2002)

Expression of eya^Scer\UAS.cBa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult.

(Bui et al., 2000)

Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of eya^Scer\UAS.cBa under the control of Scer\GAL4^dpp.blk1 results in the induction of ectopic eyes.

(Curtiss and Mlodzik, 2000)

Ectopic eyes are produced at a frequency of 10% if eya^Scer\UAS.cBa is expressed under the control of Scer\GAL4^dpp.blk1.

(Seimiya and Gehring, 2000)

10% of flies carrying a single copy of eya^Scer\UAS.cBa expressed under the control of a single copy of Scer\GAL4^dpp.blk1 in a wild-type background have ectopic eyes on the antennal segment which resemble normal eyes. These ectopic eyes contain photoreceptor cells in a pattern similar to that of the normal compound eye. 96% of flies carrying two copies of eya^Scer\UAS.cBa expressed under the control of two copies of Scer\GAL4^dpp.blk1 in a wild-type background have ectopic eyes on the antennae. 80% of these flies also show ommatidial formation on the legs, and occasionally on the wings.

(Bonini et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/eya^UAS.cBa is an enhancer of visible phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

Suppressor of

Statement

Reference

eya^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Lmx1a^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2016)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of visible | recessive phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

NOT Suppressor of

Statement

Reference

eya^UAS.cBa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Warrick et al., 1999)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has visible phenotype

(Bui et al., 2000, Seimiya and Gehring, 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

eya^UAS.cBa/Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, so^FL.UAS

(Anderson et al., 2014)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is an enhancer of proboscis phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is an enhancer of haltere phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is an enhancer of leg phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is an enhancer of wing phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is an enhancer of antenna phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Bonini et al., 1997)

Suppressor of

Statement

Reference

eya^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Lmx1a^UAS.cWa, Scer\GAL4^GMR.PU

(Wang et al., 2016)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of ommatidium phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of eye disc phenotype of dpp^d12

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of ommatidium phenotype of dpp^d12

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of eye disc | posterior | somatic clone phenotype of Mad^1-2

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of photoreceptor neuron | somatic clone phenotype of Mad^1-2

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of eye phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of eye disc morphogenetic furrow phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

Scer\GAL4^dpp.blk1/eya^UAS.cBa is a suppressor of eye disc phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

NOT Suppressor of

Statement

Reference

eya^UAS.cBa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Warrick et al., 1999)

Other

Statement

Reference

Scer\GAL4^twi.PG, Six4^UAS.cCa, eya^UAS.cBa has ventral oblique muscle cell phenotype

(Clark et al., 2006)

Scer\GAL4^twi.PG, Six4^UAS.cCa, eya^UAS.cBa has visceral mesoderm phenotype

(Clark et al., 2006)

Scer\GAL4^twi.PG, Six4^UAS.cCa, eya^UAS.cBa has embryonic somatic muscle cell phenotype

(Clark et al., 2006)

Scer\GAL4^twi.PG, Six4^UAS.cCa, eya^UAS.cBa has mesoderm phenotype

(Clark et al., 2006)

Scer\GAL4^twi.PG, Six4^UAS.cCa, eya^UAS.cBa has fat body/gonad primordium phenotype

(Clark et al., 2006)

Scer\GAL4^twi.PG, Six4^UAS.cCa, eya^UAS.cBa has trunk mesoderm phenotype

(Clark et al., 2006)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has eye | ectopic phenotype

(Bui et al., 2000, Bui et al., 2000, Seimiya and Gehring, 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has wing phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has leg phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has antenna phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, ey^UAS.cHa, eya^UAS.cBa has genitalia phenotype

(Bonini et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of eya^Scer\UAS.cBa partially suppresses eye defects seen in flies with Lmx1a^Scer\UAS.cWa driven by Scer\GAL4^GMR.PU.

(Wang et al., 2016)

Co-expression of eya^Scer\UAS.cBa and Six4^Scer\UAS.cCa in the twi domain, under the control of Scer\GAL4^twi.PG strongly affects the mesoderm. Several cell types derived from the ventral and dorsolateral mesoderm expand. Greatly increased numbers of somatic gonadal precursors results in an enlarged gonad. There are also more fat body precursors, and these now extend into the dorsal region of the mesoderm, suggesting that dorsal cells have taken on more ventral fates. In contrast, derivatives of the dorsal mesoderm are disrupted. In stage 17 embryos no myosin-expressing cardioblasts are visible, while there are gaps in the pattern of dorsal muscles. Similarly, there are gaps in the visceral mesoderm at stage 12. In the ventral region of the somatic musculature, there are duplications of the ventral acute muscle (VA3), while the pattern of ventral oblique muscles is disrupted. The lateral somatic musculature also appears larger.

(Clark et al., 2006)

Expression of eya^Scer\UAS.cBa and so^Scer\UAS.cPa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult.

(Bui et al., 2000)

Co-expression of eya^Scer\UAS.cBa and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in ectopic eye tissue at many sites, including the antenna, legs and wings.

(Bui et al., 2000)

The dpp^d-blk phenotype is partially rescued by eya^Scer\UAS.cBa expressed under the control of Scer\GAL4^dpp.blk1. The small size of dpp^d-blk/dpp^d12 eye discs is partially rescued by eya^Scer\UAS.cBa expressed under the control of Scer\GAL4^dpp.blk1 and greater numbers of developing ommatidia are seen. Eye differentiation is rescued in Mad^1-2 clones that touch the posterior margin of the eye disc when eya^Scer\UAS.cBa is expressed under the control of Scer\GAL4^dpp.blk1.

(Curtiss and Mlodzik, 2000)

Ectopic eyes are produced at a frequency of 60% if eya^Scer\UAS.cBa and so^Scer\UAS.cPa are coexpressed under the control of Scer\GAL4^dpp.blk1.

(Seimiya and Gehring, 2000)

Ectopic eye formation in flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 is greatly enhanced if the flies also carry eya^Scer\UAS.cBa; the ectopic eyes formed in flies expressing both ey^Scer\UAS.cHa and eya^Scer\UAS.cBa are larger and form with higher penetrance than in flies expressing ey^Scer\UAS.cHa alone, and eye formation occurs in the genitalia in flies expressing both ey^Scer\UAS.cHa and eya^Scer\UAS.cBa.