FlyBase Allele Report: Dmel\so[UAS.cPa]

General Information

Symbol

Dmel\so^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pignoni

FlyBase ID

FBal0083737

Feature type

allele

Associated gene

Dmel\so

Associated Insertion(s)

Carried in Construct

P{UAS-so.P}

Also Known As

UAS-So

Key Links

Transgenic product class

cDNA

(Pignoni et al., 1997)

wild_type

(Pignoni et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pignoni et al., 1997)

wild_type

(Pignoni et al., 1997)

Mutagen

in vitro construct

(Pignoni et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-so.P}

Cytology

Description

so cDNA is expressed under the control of UASt regulatory sequences.

(Pignoni et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by

(Moran et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^ey.PU

(Salzer et al., 2010)

visible, with Scer\GAL4^dpp.blk1

(Curtiss and Mlodzik, 2000)

visible, with Scer\GAL4^ey.PH

(Curtiss and Mlodzik, 2000)

visible, with Scer\GAL4^ey.PU

(Iyer et al., 2016, Salzer et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

Phenotype Manifest In

eye, with Scer\GAL4^dpp.blk1

(Curtiss and Mlodzik, 2000)

eye, with Scer\GAL4^ey.PH

(Curtiss and Mlodzik, 2000)

eye, with Scer\GAL4^ey.PU

(Iyer et al., 2016, Salzer et al., 2010)

eye, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

ommatidium, with Scer\GAL4^dpp.blk1

(Curtiss and Mlodzik, 2000)

ommatidium, with Scer\GAL4^ey.PH

(Curtiss and Mlodzik, 2000)

Detailed Description

Statement

Reference

Flies expressing so^UAS.cPa under the control of either Scer\GAL4^GMR.PU or Scer\GAL4^ey.PU have small, rough eyes.

(Iyer et al., 2016)

Eyes expressing so^Scer\UAS.cPa driven by Scer\GAL4^ey.PU are smaller than controls.

(Salzer et al., 2010)

Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^ey.PH or Scer\GAL4^dpp.blk1 results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes.

(Curtiss and Mlodzik, 2000)

Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 does not result in ectopic eye formation.

(Seimiya and Gehring, 2000)

Ectopic expression of so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 has little or no effect on antennal, wing or leg disc development.

(Pignoni et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(3R)Exel6211

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(3R)BSC806

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, enhanceable by Cpsf100^f00376

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(3R)Exel6180

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(2R)CX1

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(2L)spd^j2

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(3R)by10

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, enhanceable by Df(2R)Jp8

(Iyer et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, suppressible by Df(3R)BSC501

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, suppressible by Df(3R)ED6316

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, suppressible by Chd1^HMS01142

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has visible phenotype, suppressible by Df(3R)XTA1

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, suppressible by Df(2L)BSC109

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, suppressible by Df(2L)BSC37

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, suppressible by Df(1)g

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has visible phenotype, suppressible by Df(1)sd72b

(Iyer et al., 2016)

Enhancer of

Statement

Reference

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of visible | recessive phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

so^UAS.cPa/Scer\GAL4^ey.PH is an enhancer of visible | recessive phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of visible phenotype of Scer\GAL4^dpp.blk1, eya^UAS.cPa

(Pignoni et al., 1997)

Other

Statement

Reference

Scer\GAL4^Act.PU, eya^UAS.cUa, so^UAS.cPa has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype

(Apitz and Salecker, 2016)

Scer\GAL4^dpp.blk1, eya^E7.UAS, so^UAS.cPa has visible phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E11.UAS, so^UAS.cPa has visible phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has visible phenotype

(Bui et al., 2000, Seimiya and Gehring, 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(3R)Exel6211

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(3R)BSC806

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, enhanceable by Cpsf100^f00376

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(3R)Exel6180

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(2R)CX1

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(2L)spd^j2

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(3R)by10

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, enhanceable by Df(2R)Jp8

(Iyer et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, suppressible by Df(2L)BSC37

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, suppressible by Df(1)g

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, suppressible by Df(1)sd72b

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, suppressible by Df(3R)BSC501

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, suppressible by Df(3R)ED6316

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, suppressible by Chd1^HMS01142

(Iyer et al., 2016)

Scer\GAL4^ey.PU, so^UAS.cPa has eye phenotype, suppressible by Df(3R)XTA1

(Iyer et al., 2016)

Scer\GAL4^GMR.PU, so^UAS.cPa has eye phenotype, suppressible by Df(2L)BSC109

(Iyer et al., 2016)

Enhancer of

Statement

Reference

so^UAS.cPa/Scer\GAL4^30A is an enhancer of wing disc phenotype of Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa

(Pappu et al., 2003)

so^UAS.cPa/Scer\GAL4^30A is an enhancer of photoreceptor neuron | ectopic phenotype of Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa

(Pappu et al., 2003)

so^UAS.cPa/Scer\GAL4^ey.PH is an enhancer of eye phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of eye phenotype of dpp^d-blk

(Curtiss and Mlodzik, 2000)

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of antennal disc phenotype of Scer\GAL4^dpp.blk1, eya^UAS.cPa

(Pignoni et al., 1997)

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of leg phenotype of Scer\GAL4^dpp.blk1, eya^UAS.cPa

(Pignoni et al., 1997)

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of antenna phenotype of Scer\GAL4^dpp.blk1, eya^UAS.cPa

(Pignoni et al., 1997)

so^UAS.cPa/Scer\GAL4^dpp.blk1 is an enhancer of wing phenotype of Scer\GAL4^dpp.blk1, eya^UAS.cPa

(Pignoni et al., 1997)

NOT Enhancer of

Statement

Reference

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

NOT Suppressor of

Statement

Reference

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Other

Statement

Reference

Scer\GAL4^Act.PU, eya^UAS.cUa, so^UAS.cPa has embryonic/larval optic lobe | somatic clone | third instar larval stage phenotype

(Apitz and Salecker, 2016)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has eye | ectopic phenotype

(Bui et al., 2000, Bui et al., 2000, Seimiya and Gehring, 2000)

Scer\GAL4^dpp.blk1, eya^E7.UAS, so^UAS.cPa has eye phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E7.UAS, so^UAS.cPa has eye | ectopic phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has leg phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has antenna phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E11.UAS, so^UAS.cPa has leg phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E7.UAS, so^UAS.cPa has leg phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E11.UAS, so^UAS.cPa has antenna phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E11.UAS, so^UAS.cPa has pigment cell | ectopic phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E7.UAS, so^UAS.cPa has antenna phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^E7.UAS, so^UAS.cPa has ommatidium | ectopic phenotype

(Bui et al., 2000)

Scer\GAL4^dpp.blk1, eya^UAS.cBa, so^UAS.cPa has wing phenotype

(Bui et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of eya^Scer\UAS.cUa together with so^Scer\UAS.cPa under the control of Scer\GAL4^Act.PU in somatic clones in the brain optic lobes confers outer proliferation centre (OPC)-like identity to the p-IPC (proximal inner proliferation centre - gives rise to migratory progenitors) and induces their conversion to neuroblasts.

(Apitz and Salecker, 2016)

Clones of so^Scer\UAS.cPa; eya^Scer\UAS.cPa; Scer\GAL4^Act5C.PP cells in the testes do not induce precocious spermatocyte development.

(Fabrizio et al., 2003)

When dpp^Scer\UAS.cSa, eya^Scer\UAS.cPa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in both compartments of the wing disc. The addition of so^Scer\UAS.cPa increases the penetrance of this phenotype. The addition of so^Scer\UAS.cPa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also not affected. The addition of dpp^Scer\UAS.cSa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued. If so^Scer\UAS.cPa is also added, posterior margin clones are still rescued, although ectopic anterior furrows are induced with high frequency. The addition of so^Scer\UAS.cPa and dpp^Scer\UAS.cSa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs.

(Pappu et al., 2003)

Expression of eya^Scer\UAS.cBa and so^Scer\UAS.cPa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult.

(Bui et al., 2000)

Co-expression of eya^Scer\UAS.cBa and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in ectopic eye tissue at many sites, including the antenna, legs and wings. Co-expression of eya^E7.Scer\UAS and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in ectopic retinal tissue in 19% of cases. The ectopic retinal tissue consists of discernible ommatidia and is limited to the antennal region. Abnormal leg development is seen in 100% of cases, and in 12% of cases a reduced eye phenotype is seen. Co-expression of eya^E11.Scer\UAS and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in ectopic retinal tissue in 51% of cases. The ectopic retinal tissue is limited to specks of pigment on the tips of the antenna. Abnormal leg development is seen in 100% of cases.

(Bui et al., 2000)

The dpp^d-blk small eye phenotype is enhanced by so^Scer\UAS.cPa expressed under the control of either Scer\GAL4^dpp.blk1 or Scer\GAL4^ey.PH, which often results in a complete lack of eye development.

(Curtiss and Mlodzik, 2000)

Ectopic eyes are produced at a frequency of 60% if eya^Scer\UAS.cBa and so^Scer\UAS.cPa are coexpressed under the control of Scer\GAL4^dpp.blk1.

(Seimiya and Gehring, 2000)

Coexpression of both eya^Scer\UAS.cPa and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in a dramatic increase in the development of eye tissue in antennal discs compared to expression of eya^Scer\UAS.cPa alone under the control of Scer\GAL4^dpp.blk1. This tissue develops into adult eye structures. There is also an increase in the frequency and size of red pigmented patches on the wings and legs. Ectopic eyes are not produced when eya^Scer\UAS.cPa and so^Scer\UAS.cPa are coexpressed under the control of Scer\GAL4^dpp.blk1 in an ey² background, although growth alterations and patches of red pigment cells on the legs are still seen.

(Pignoni et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

so^UAS.cPa/Scer\GAL4^hs.PB rescues so¹

(Kenyon et al., 2005)

Partially rescues

so^UAS.cPa/Scer\GAL4^{so.10.EY.TOYmt.1.2.5} partially rescues so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^so.10.TOYmt partially rescues so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^{so.7.EY.TOYmt.1.2.5} partially rescues so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^so.7.TOYmt partially rescues so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^so.7 partially rescues so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^so.10 partially rescues so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^h-1J3 partially rescues so³

(Suzuki and Saigo, 2000)

so^UAS.cPa/Scer\GAL4^upd1-E132 partially rescues so¹

(Pignoni et al., 1997)

so^UAS.cPa/Scer\GAL4^dpp.blk1 partially rescues so¹

(Pignoni et al., 1997)

Fails to rescue

so^UAS.cPa/Scer\GAL4^{so.10.EY+TOYmt.1-5} fails to rescue so¹

(Punzo et al., 2002)

so^UAS.cPa/Scer\GAL4^{so.7.EY+TOYmt.1-5} fails to rescue so¹

(Punzo et al., 2002)

Comments

Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^so.10 or Scer\GAL4^so.10.TOYmt rescues the missing eyes of so¹ animals, but not the ocelli. Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^{so.10.EY.TOYmt.1.2.5} only partially rescues the missing eyes of so¹ animals; almost 100% of the flies have a strongly reduced eye on one side of the head and no eye on the other side. Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^so.7 fully rescues the missing eyes of so¹ animals, and partially rescues the ocelli. Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^{so.7.EY.TOYmt.1.2.5} does not rescue the missing eyes of so¹ animals but rescues the ocelli. Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^so.7.TOYmt rescues the missing eyes of so¹ animals but does not rescue the ocelli. Expression of so^Scer\UAS.cPa under the control of Scer\GAL4^{so.7.EY+TOYmt.1-5} does not rescue the missing eyes or ocelli of so¹ animals.

(Punzo et al., 2002)

The lack of Bolwig's organ neurons seen in so³ embryos is partially rescued by so^Scer\UAS.cPa expressed under the control of Scer\GAL4^h-1J3.

(Suzuki and Saigo, 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26808

w^*; P{UAS-so.P}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

so^Scer\UAS.cPa

so^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pignoni

(Pignoni et al., 1997)

Secondary FlyBase IDs

References (23)

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