FlyBase Allele Report: Dmel\amn[X8]

General Information

Symbol

Dmel\amn^X8

Species

D. melanogaster

Name

FlyBase ID

FBal0090485

Feature type

allele

Associated gene

Dmel\amn

Associated Insertion(s)

P{?GawB}amn^X8

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Keene et al., 2006)

Mutagen

Delta2-3 transposase

(Moore et al., 1998)

Progenitor genotype

amn^28A

(Moore et al., 1998)

Associated Insertion(s)

P{?GawB}amn^X8

Cytology

Description

Imprecise excision of the P{GawB} insertion that retains full Scer\GAL4 activity.

(Caldwell et al., 2005)

Imprecise excision of the P{w⁺} element, resulting in a deletion of approximately 0.8kb that removes the open reading frame.

(Moore et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Moore et al., 1998)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

CNS surface glial cell

(Yager et al., 2001)

Kenyon cell

(Rosay et al., 2001)

Kenyon cell (with amn^c651)

(Rosay et al., 2001)

Detailed Description

Statement

Reference

Three-hour memory impairment after cold-induced anesthesia is not affected in amn^X8 mutants. In contrast, reducing 5HT levels by pCPA feeding reduces 3 hour memory in amn^X8 mutants.

Twenty-four hour memory in amn^X8 mutants is impaired after 10 sessions of spaced training but not after massed training.

(Lee et al., 2011)

amn^X8 mutant larvae show a significant delay in response to noxious heat compared to control flies. These flies also exhibit a delay in their jump response to noxious heat of 9 seconds.

amn^28A/amn^X8 females, both larvae and adults, display a prolonged latency in their responses to a noxious heat stimulus.

(Aldrich et al., 2010)

amn^X8 mutants make choies according to their recent experience and completely ignore the past experience in a 60-60V 1hr delay choice, similar to wild-type. No significant difference is observed between the 1hr Anesthesia-resistant memory (ARM) of a single conditioning event and that followed by a second conditioning event in amn^X8 mutants.

The choice PI in the 60-30 V 1hr delay protocol is not significantly different from the 30 V immediate memory PI in amn^X8 mutants, compared to wild-type where it is significantly lower.

(Yin et al., 2009)

amn^X8 and amn^X8/amn¹ flies are able to learn to associate an odor with a sugar reward (although they have a small but significant initial performance defect), but they forget this association within 60 minutes of training.

(Keene et al., 2006)

amn^X8 flies have a defective olfactory memory. These flies fail to produce the increased calcium influx seen in the dorsal paired medial neurons of wild-type flies at 30 minutes after a conditioning of 3s exposure to odour, followed by a 60s odour stimulus applied simultaneously with 12 electric-shock pulses. Additionally, amn^X8 flies exhibit poor 3 hour memory following odorant training, irrespective of the duration of temperature shifts applied after training. amn^X8 flies show no difference to wild-type flies in their performance after 1 to 15 short training trials, consisting of 10s of CS odour, a single electric shock 9s late, 30s fresh air and a further 10s of CS odor. However, amn^X8 flies' performance is lower than wild type when tested 2 hours after 10 training trials.

(Yu et al., 2005)

Muscles of third instar amn^X8 mutant larvae show a reduction in the L-type Ca²⁺ current compared to control larvae.

(Bhattacharya et al., 2004)

amn^X8 flies exhibit both short-term (3 min) and long term (3 hr) memory loss.

While OCT learning is indistinguishable from wild-type, amn^X8 flies exhibit greatly reduced BA learning.

amn^X8 mutant flies exhibit a significant MCH immediate memory defect.

amn^X8 flies without olfactory organs display BA avoidance that is indistinguishable from wild-type flies lacking olfactory organs.

amn^X8;Scer\GAL4^c316/amn^Scer\UAS.cWa and amn^X8;Scer\GAL4^Mz717/amn^Scer\UAS.cWa flies learn to avoid benzaldehyde significantly better than amn^X8 flies, although their performance is still significantly worse than that of wild-type flies. In contrast, OCT immediate memory of amn^X8 flies is indistinguishable from wild-type flies and amn^X8;Scer\GAL4^c316/amn^Scer\UAS.cWa and amn^X8;Scer\GAL4^Mz717/amn^Scer\UAS.cWa flies.

(Keene et al., 2004)

Mutant animals, unlike wild-type, do not show significant age-related memory impairment, 1 hour memory in aged flies was not significantly different from young mutant flies.

(Tamura et al., 2003)

In amn^X8 homozygotes or amn^X8/amn^c651 transheterozygotes the amplitude of the synchronous oscillation of intracellular calcium concentration seen in wild type Kenyon cells is significantly increased.

(Rosay et al., 2001)

Mutants exhibit increased perineurial glial thickness.

(Yager et al., 2001)

Shows defects in memory retention both immediately (initial learning) or 180 minutes (3 hour memory) after training.

(DeZazzo et al., 1999)

Hemizygous males and homozygous females show increased sensitivity to ethanol in an inebriometer assay.

(Moore et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

amn^28A

(DeZazzo et al., 1999)

amn¹

(DeZazzo et al., 1999)

amn^chpd

(Moore et al., 1998)

Rescued by

amn^X8/amn^28A is rescued by amn^UAS.cWa/Scer\GAL4^da.G32

(Aldrich et al., 2010)

amn^X8/amn¹ is rescued by amn^UAS.cWa/Scer\GAL4^c316

(Keene et al., 2006)

amn^X8 is rescued by amn^UAS.cWa/Scer\GAL4^c316

(Yu et al., 2005)

amn^X8 is rescued by amn^hs.PM

(Bhattacharya et al., 2004)

amn^X8 is rescued by Scer\GAL4^c316/amn^UAS.cDa

(Tamura et al., 2003)

amn^X8 is rescued by amn^hs.PM

(Yager et al., 2001)

amn^X8 is rescued by amn^UAS.cWa/Scer\GAL4^c316

(Waddell et al., 2000)

amn^X8 is rescued by amn^hs.PM

(Moore et al., 1998)

Partially rescued by

amn^X8 is partially rescued by amn^hs.PM

(DeZazzo et al., 1999)

Not rescued by

amn^X8 is not rescued by amn^UAS.cWa/Scer\GAL4^c309

(Waddell et al., 2000)

amn^X8 is not rescued by Scer\GAL4^c747/amn^UAS.cWa

(Waddell et al., 2000)

Comments

The presence of amn^Scer\UAS.cWa under the control of Scer\GAL4^da.G32 significantly rescues the delayed larval and adult response to noxious heat in amn^28A/amn^X8 females.

(Aldrich et al., 2010)

amn¹, amn^X8 and amn^28A all fail to complement each other for memory retention either immediately (initial learning) or 180 minutes (3 hour memory) after training.

(DeZazzo et al., 1999)

The ethanol sensitive phenotype is rescued by amn^hs.PM expressed using heat shock. Three heat shocks at 24 hour intervals in adult flies is sufficient to fully rescue the ethanol-sensitive phenotype.

(Moore et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

amn^X8

(Turrel et al., 2020, Turrel et al., 2018, Chen et al., 2015, Aldrich et al., 2010, Yin et al., 2009, Yamazaki et al., 2007, Keene et al., 2006, Yu et al., 2005, Keene et al., 2004)

amn^x8

(Bhattacharya et al., 2004, Tamura et al., 2003, Yager et al., 2000)

Name Synonyms

amnesiacX8

(Lee et al., 2011)

Secondary FlyBase IDs

References (20)

Report Sections

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