FlyBase Allele Report: Dmel\N[ECN.UAS.cGa]

General Information

Symbol

Dmel\N^ECN.UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0090564

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.ECN.G}

Also Known As

UAS-N^DN, UAS-NEXT, UAS-Notch^DN, N^ecd, UAS-dnNotch

Key Links

Transgenic product class

dominant_negative_variant

(Go et al., 1998)

inframe_deletion

(Go et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Go et al., 1998)

inframe_deletion

(Go et al., 1998)

Mutagen

in vitro construct

(Go et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-N.ECN.G}

Cytology

Description

UAS regulatory sequences drive expression of a dominant negative form of N in which most of the intracellular domain is deleted.

(Go et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage | somatic clone

(Vaccari et al., 2010)

decreased occurrence of cell division | larval stage, with Scer\GAL4^dpp.blk1

(Ku and Sun, 2017)

increased cell number | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Vaccari et al., 2010)

visible, with Scer\GAL4^ey.PH

(Lee et al., 2009)

visible, with Scer\GAL4^vg.int2.1

(Go et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

Third instar larval eye-antennal discs expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^hth-GETDB show a disruption of epithelial folding at the A1 fold, and abnormal mixing of cells on either side of the A1 field boundary, as compared to controls.

Third instar larval eye-antennal disc clones expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^Act5C.PI, and not spanning the A1 fold, do not show abnormal mixing of cells on either side of the A1 field boundary, but cells show a larger apical area than those normally found in the fold.

Larval eye-antennal discs expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^dpp.blk1 exhibit decreased cell proliferation, but no significant change in apoptosis levels, as compared to controls.

(Ku and Sun, 2017)

Expression of N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} has no significant effect on the number of abdominal lateral adult muscle precursor cells in mid-third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Expression of N^{ECN.Scer\UAS.cGa} in the eye disc under the control of Scer\GAL4^Act5C.PP results in clones that overproliferate compared to wild type cells.

(Vaccari et al., 2010)

Expression of N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^ey.PH results in a dramatic loss of the eye.

(Lee et al., 2009)

N^{ECN.Scer\UAS.cGa}; Scer\GAL4^ey.PH flies lack eyes.

(Chao et al., 2004)

N^{ECN.Scer\UAS.cGa}; Scer\GAL4¹¹⁵¹ pupae have normal numbers of dorso-ventral and medio-lateral indirect flight muscle founder myoblasts and the resulting adults have the correct number of dorso-ventral and medio-lateral indirect flight muscle fibres.

(Dutta et al., 2004)

Lymph glands that express (driven by Scer\GAL4^{Scer\FRT.Act5C}) are smaller than wild-type. This phenotype is not due to increased apoptosis.

(Lebestky et al., 2003)

When N^{ECN.Scer\UAS.cGa} expressing clones (driven by Scer\GAL4^Act5C.PP) are made in the ventral part of the wing disc, they are not significantly rounder than wild-type clone.

(Milan and Cohen, 2003)

Neuronal patterning in the ommatidial preclusters is maintained in flies expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^lz-gal4.

(Flores et al., 2000)

Expression of N^{ECN.Scer\UAS.cGa} driven by Scer\GAL4^ey.PH leads to a severely reduced eye.

(Kurata et al., 2000)

Over expression of the dominant negative form of N, N^{ECN.Scer\UAS.cGa} in the posterior compartment of the wing disc by Scer\GAL4^en-e16E, produces a ragged dorsal/ventral boundary.

(Micchelli and Blair, 1999)

Flies expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^vg.int2.1 have wing defects ranging from wing margin notches to rudimentary wings.

(Go et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^ECN.UAS.cGa has abnormal size | somatic clone | larval stage phenotype, suppressible by Vha55^j2E9

(Vaccari et al., 2010)

N^ECN.UAS.cGa, Scer\GAL4^Act5C.PP has increased cell number | somatic clone | larval stage phenotype, suppressible by Vha55^j2E9

(Vaccari et al., 2010)

N^ECN.UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by Hipk^UAS.Tag:HA, Scer\GAL4^ey.PH

(Lee et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PU/N^ECN.UAS.cGa is a suppressor | partially of abnormal cell polarity | recessive | somatic clone phenotype of flw^FP41

(Sun et al., 2011)

NOT Suppressor of

Statement

Reference

N^ECN.UAS.cGa, Scer\GAL4^ppk.PG is a non-suppressor of abnormal neuroanatomy | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

N^ECN.UAS.cGa/Scer\GAL4^Act5C.PP is a non-suppressor of decreased cell number | somatic clone | larval stage phenotype of Vha55^j2E9

(Vaccari et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^ECN.UAS.cGa, Scer\GAL4^hth-GETDB has eye-antennal disc | third instar larval stage phenotype, suppressible by mir-ban^{sponge.UAS.DsRed(Unk)}, Scer\GAL4^hth-GETDB

(Ku and Sun, 2017)

N^ECN.UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by Hipk^UAS.Tag:HA, Scer\GAL4^ey.PH

(Lee et al., 2009)

N^ECN.UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by eyg^UAS.lune, Scer\GAL4^ey.PH

(Chao et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PU/N^ECN.UAS.cGa is a suppressor | partially of oocyte | somatic clone phenotype of flw^FP41

(Sun et al., 2011)

N^ECN.UAS.cGa, Scer\GAL4^Act5C.PP is a suppressor | partially of wing disc | somatic clone phenotype of Bx^EP1306, Scer\GAL4^Act5C.PP

(Milan and Cohen, 2003)

NOT Suppressor of

Statement

Reference

N^ECN.UAS.cGa, Scer\GAL4^ppk.PG is a non-suppressor of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

N^ECN.UAS.cGa/Scer\GAL4^Act5C.PP is a non-suppressor of eye disc | somatic clone phenotype of Vha55^j2E9

(Vaccari et al., 2010)

Other

Statement

Reference

N^ECN.UAS.cGa, Scer\GAL4^dpp.blk1, toy^UAS.cCa has eye | ectopic phenotype

(Kurata et al., 2000)

N^ECN.UAS.cGa, Scer\GAL4^dpp.blk1, ey^UAS.cHa has eye | ectopic phenotype

(Kurata et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ban^{sponge.Scer\UAS.T:Disc\RFP} suppresses the disrupted A1 epithelial folding and cell mixing at the A1 field boundary seen in eye-antennal discs expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^hth-GETDB.

(Ku and Sun, 2017)

Expression of N^{ECN.Scer\UAS.cGa} does not suppress the dendrite pruning defects seen in ddaC neurons expressing lin19^GD9650 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

(Wong et al., 2013)

Expression of N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flw^FP41.

(Sun et al., 2011)

Eye disc clones expressing N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^Act5C.PP in a Vha55^j2E9 mutant background do not proliferate as in a wild-type background and instead create only small clones, of a similar size to Vha55^j2E9 mutant clones.

(Vaccari et al., 2010)

The eye loss phenotype caused by expression of N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^ey.PH is partially suppressed by co-expression of hipk^{Scer\UAS.T:Ivir\HA1}.

(Lee et al., 2009)

Eye development in N^{ECN.Scer\UAS.cGa}; Scer\GAL4^ey.PH flies is partially rescued by eyg^{Scer\UAS.lune}. The resulting eyes are almost as big as wild-type.

(Chao et al., 2004)

Flies expressing N^{ECN.Scer\UAS.cGa} and either toy^Scer\UAS.cCa or ey^Scer\UAS.cHa driven by Scer\GAL4^dpp.blk1 exhibit ectopic eyes on legs and wings in all flies.

(Kurata et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^{ECN.Scer\UAS.cGa}

N^ECN.UAS.cGa

N^ecd

(Herranz et al., 2006, Milan and Cohen, 2003)

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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