FlyBase Allele Report: Dmel\tsh[UAS.cGa]

tsh^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PF results in roughening of the posterior portion of the adult eye as a result of nonspecific loss of photoreceptor cells within each ommatidium.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.PS induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^cb26 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^cb41 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^Ser.PGF induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^c329b does not induce ectopic eye formation.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^C833 does not induce ectopic eye formation.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^MJ33a does not induce ectopic eye formation.

tsh^Scer\UAS.cGa under the control of Scer\GAL4^T98 does not induce ectopic eye formation.

(Datta et al., 2009)

Clones expressing tsh^Scer\UAS.cGa, driven by Scer\GAL4^αTub84B.PZ, in the eye disc margin or peripodial epithelium induce the overproliferation and columnarization of cells. Some of these clones show a sorting behaviour, with tsh^{dsRNA.Scer\UAS}-expressing cells arranging themselves as hollow sacs with their apical sides pointing inwards. Transient tsh^Scer\UAS.cGa expression, driven by Scer\GAL4^hs.PB following a heat shock to L2 larvae, results in the development of distinct ectopic clusters of photoreceptor cells in the peripodial epithelium. Such ectopic photoreceptors are seen when tsh^Scer\UAS.cGa is expressed in the margin and the peripodial epithelium of the L3 disc by Scer\GAL4^Bx-MS1096 or Scer\GAL4^MD705. As adults these flies exhibit ectopic eyes. The Scer\GAL4^Bx-MS1096>tsh^Scer\UAS.cGa adults have ventral ectopic eyes are induced that replace ventral head structures and also show small dorsal patches of photoreceptors on the ventral head, which are normally accompanied by a bristle.

(Bessa and Casares, 2005)

Ectopic expression of tsh^Scer\UAS.cGa in the epidermis at 25^oC, driven by Scer\GAL4^69B, causes a homeotic labial to T1 segment transformation, which is visible due to the formation of a denticle T1 "beard" in the labial segment. The maxillary and antennal segments also take on a trunk-like identity, shown by the development of ectopic denticles. Head involution is defective in these mutants and much of the head skeleton is missing. Additionally, these mutants experience a loss of denticles on the trunk.

(Laugier et al., 2005)

Changes in cuticle pattern indicate that in tsh^Scer\UAS.cGa; Scer\GAL4^69B embryos, the labial segment is transformed to T1 and the antennal and maxillary head segments are transformed towards a trunk identity in that they develop denticles and naked cuticle. tsh^Scer\UAS.cGa; Scer\GAL4^dpp.blk1 flies have an ectopic eye ventral to each of their eyes, whereas in tsh^Scer\UAS.cGa; Scer\GAL4^ey.PH flies the eyes are deformed.

(Manfroid et al., 2004)

When tsh^Scer\UAS.cGa is driven by Scer\GAL4^69B, completely suppresses the formation of denticles.

(Angelats et al., 2002)

Clones of cells in the eye disc expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^αTub84B.PZ grow poorly in the middle of the disc but can induce large overgrowths at the edge of the disc.

(Bessa et al., 2002)

Pharate adults expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 show splitting of the endogenous eye in 8% of cases and sometimes have an ectopic eye at the base of the antenna. 42% of animals expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 arrest and die at the white pupal stage. These animals are about 50% larger than normal. These animals have an extended larval period (which is about 2-3 days longer than normal) and their eye-antennal discs show both overgrowth and distorted morphology. When two copies of tsh^Scer\UAS.cGa are expressed under the control of Scer\GAL4^dpp.blk1, the ventral half of the endogenous eye is missing in all flies. Expression of tsh^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH results in complete suppression of eye development in 97% of cases. Expression of tsh^Scer\UAS.cGa under the control of Scer\GAL4^bi-omb-Gal4 results in absence of the ventral eye in pharate adults and overgrowth of the dorsal eye. The eye disc is enlarged in these animals. Expression of tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI can suppress photoreceptor development in the eye disc, but only along the ventral margin. Clones in the dorsal margin of the eye disc result in overgrowth. Clones in the internal part of the eye, away from the margins, do not affect eye fate. The frequency of dorsal clones is higher than that of ventral clones. Adults in which clones expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI have been induced show suppression of the ventral eye or overgrowth of the dorsal eye. Expression of tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 or Scer\GAL4^bi-omb-Gal4 results in splitting of the wing pouch. Expression of tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI results in duplications of antennal segments distal to AN2. Clones in the anteroproximodorsal region of the antennal disc cause ectopic eye formation.

(Singh et al., 2002)

When tsh^Scer\UAS.cGa is driven by Scer\GAL4^48Y no larvae hatch when reared at 25^oC. When reared at 20^oC a few escaper larvae are found. In these animals no copper cells are seen in the larval gut.

(Waltzer et al., 2001)

Expression of the P{UAS-tsh.G}20 insertion under the control of Scer\GAL4^69B does not affect denticle formation at 25^oC.

(Gallet et al., 2000)

Somatic clones ectopically expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PP or Scer\GAL4^{arm.T:Hsim\VP16} in the coxa and trochanter are wild-type. No clones are seen in ventral positions in the leg, however, vesicles consisting of bristleless cuticle are formed. Vesicles of cuticle are also formed in the lumen of the leg resulting in a reduction of femur length and a perturbation at the boundary region in any dorsal-ventral position. When clones are induced in third instar larvae, outgrowths are seen especially in dorsal positions. In distal parts of the femur, clones are observed within which the bristles lacked bracts. After a longer clone induction time, clones located in ventral positions are always abnormal, producing fusion between femur and tibia. Somatic clones ectopically expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PP or Scer\GAL4^{arm.T:Hsim\VP16} in the leg disc. two or three large clones per disc are seen in the endogenous tsh domain, mostly isolated cells are detected in the distal domain. these cells often sort out from their distal neighbors towards the lumen but remain attached to the epithelium of the disc. When induction time is increased, groups of tsh positive cells extend into the distal domain.

(Erkner et al., 1999)

Expression of tsh^Scer\UAS.cGa under the control of Scer\GAL4^69B results in embryos in which the head segments have trunk identity. These embryos have naked cuticle with almost no ventral denticle belts. The prothoracic beard is not present. Overexpression of tsh^Scer\UAS.cGa in embryos using Scer\GAL4^hs.PB between stages 9 and 10 results in a naked cuticle phenotype.

(Gallet et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PZ, tsh^UAS.cGa has increased cell number | somatic clone | larval stage phenotype, suppressible by Axn^A2.UAS, Scer\GAL4^αTub84B.PZ

(Bessa and Casares, 2005)

Scer\GAL4^dpp.blk1, tsh^UAS.cGa has lethal | pupal stage phenotype, suppressible | partially by hth^dtl-S142204/hth[+]

(Singh et al., 2002)

Scer\GAL4^dpp.blk1, tsh^UAS.cGa has lethal | pupal stage phenotype, suppressible | partially by wg^l-12/wg[+]

(Singh et al., 2002)

Scer\GAL4^dpp.blk1, tsh^UAS.cGa has visible phenotype, suppressible by wg^l-12/wg[+]

(Singh et al., 2002)

Scer\GAL4^dpp.blk1, tsh^UAS.cGa has visible phenotype, suppressible by hth^dtl-S142204/hth[+]

(Singh et al., 2002)

Other

Statement

Reference

Scer\GAL4^VP16.arm, arm^{S10.UAS.Tag:MYC}, tsh^UAS.cGa has lethal | somatic clone phenotype

(Erkner et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, tsh^UAS.cGa has eye phenotype, suppressible by hth^dtl-S142204/hth[+]

(Singh et al., 2002)

Scer\GAL4^dpp.blk1, tsh^UAS.cGa has eye phenotype, suppressible by wg^l-12/wg[+]

(Singh et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^69B, tsh^UAS.cGa has denticle phenotype, non-suppressible by ci⁹⁴/ci⁹⁴

(Angelats et al., 2002)

Enhancer of

Statement

Reference

tsh^UAS.cGa, Scer\GAL4^bi-omb-Gal4 is an enhancer of eye disc | ventral phenotype of Delta^DN.UAS, Scer\GAL4^bi-omb-Gal4

(Singh et al., 2004)

tsh^UAS.cGa, Scer\GAL4^bi-omb-Gal4 is an enhancer of eye disc | dorsal phenotype of Delta^DN.UAS, Scer\GAL4^bi-omb-Gal4

(Singh et al., 2004)

Suppressor of

Statement

Reference

tsh^UAS.cGa/Scer\GAL4^bi-omb-Gal4 is a suppressor | partially of eye disc | ventral phenotype of Scer\GAL4^bi-omb-Gal4, Ser^{Bd.UAS.Tag:MYC}

(Singh et al., 2004)

tsh^UAS.cGa/Scer\GAL4^bi-omb-Gal4 is a suppressor | partially of eye disc | dorsal phenotype of Scer\GAL4^bi-omb-Gal4, Ser^{Bd.UAS.Tag:MYC}

(Singh et al., 2004)

tsh^UAS.cGa/Scer\GAL4^69B is a suppressor of ventral denticle belt phenotype of ci⁹⁴

(Angelats et al., 2002)

tsh^UAS.cGa/Scer\GAL4^69B is a suppressor of embryonic mesothoracic segment & sclerite | ectopic phenotype of spen^poc361

(Wiellette et al., 1999)

tsh^UAS.cGa/Scer\GAL4^69B is a suppressor of embryonic metathoracic segment & sclerite | ectopic phenotype of spen^poc361

(Wiellette et al., 1999)

tsh^UAS.cGa/Scer\GAL4^69B is a suppressor of embryonic/first instar larval cuticle phenotype of arm²

(Gallet et al., 1998)

NOT Suppressor of

Statement

Reference

tsh^UAS.cGa/Scer\GAL4^69B, ci⁹⁴/ci⁹⁴ is a non-suppressor of denticle phenotype of Scer\GAL4^69B, arm^{S10.UAS.Tag:MYC}

(Angelats et al., 2002)

Other

Statement

Reference

Delta^DN.UAS, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye | ventral phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^UAS.cSa, tsh^UAS.cGa has eye disc phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^UAS.cSa, tsh^UAS.cGa has eye | ventral phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^UAS.cSa, tsh^UAS.cGa has eye disc | dorsal phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^UAS.cSa, tsh^UAS.cGa has eye disc | ventral phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^UAS.cSa, tsh^UAS.cGa has eye | dorsal phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, ara^UAS.cGa, tsh^UAS.cGa has eye | ventral phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, ara^UAS.cGa, tsh^UAS.cGa has eye disc | dorsal phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, ara^UAS.cGa, tsh^UAS.cGa has eye disc | ventral phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, ara^UAS.cGa, tsh^UAS.cGa has eye | dorsal phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye disc | dorsal phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye disc | ventral phenotype

(Singh et al., 2004)

Df(3L)iro-DFM3, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | dorsal | somatic clone phenotype

(Singh et al., 2004)

Df(3L)iro-DFM3, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | ventral | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye | dorsal phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | dorsal | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | ventral | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye | dorsal | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye | ventral | somatic clone phenotype

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^UAS.cSa, tsh^UAS.cGa has eye phenotype

(Singh et al., 2004)

Scer\GAL4^αTub84B.PZ, hth^UAS.GFP, tsh^UAS.cGa has eye disc | somatic clone phenotype

(Bessa et al., 2002)

Scer\GAL4^Act5C.PI, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}, tsh^UAS.cGa has eye phenotype

(Singh et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tsh^Scer\UAS.cGa and ara^Scer\UAS.cGa under the control of Scer\GAL4^bi-omb-Gal4 results in enlargement at both the dorsal and ventral margins of the eye disc and adult eye.

Co-expression of tsh^Scer\UAS.cGa and ara^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI in clones in the eye disc does not affect eye fate.

The eye field is suppressed on both dorsal and ventral margins in eye discs expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI in clones in the eye disc in a Df(3L)iro-DFM3 background.

Co-expression of tsh^Scer\UAS.cGa and Dl^Scer\UAS.cHa under the control of Scer\GAL4^bi-omb-Gal4 results in eye enlargements on both dorsal and ventral margins in both the eye disc and the adult eye.

Ventrally and dorsally located clones in the eye co-expressing tsh^Scer\UAS.cGa and Dl^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PI result in overgrowth.

Co-expression of tsh^Scer\UAS.cGa further enhances the suppression of eye development at the dorsal and ventral margins of the eye disc which is seen in animals expressing Dl^D.Scer\UAS under the control of Scer\GAL4^bi-omb-Gal4.

Clones in the dorsal part of the eye disc which are co-expressing tsh^Scer\UAS.cGa and Dl^D.Scer\UAS under the control of Scer\GAL4^Act5C.PI cause autonomous overgrowth in the posterior margin of the dorsal eye, but photoreceptor differentiation is suppressed within the clone. The overall size of the eye disc is also reduced.

Co-expression of both tsh^Scer\UAS.cGa and Ser^Scer\UAS.cSa under the control of Scer\GAL4^bi-omb-Gal4 suppresses eye development in both the dorsal and ventral margins of the eye disc and adult eye. However, despite this suppression of photoreceptor differentiation, the eye disc shows overall enlargement and the adult eye is also enlarged and folded.

Co-expression of tsh^Scer\UAS.cGa partially rescues the suppression of eye development on both the ventral and dorsal margins of the eye that is seen in animals expressing Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^bi-omb-Gal4.

Clones in the ventral part of the eye that are co-expressing tsh^Scer\UAS.cGa and fng^Scer\UAS.cKa under the control of Scer\GAL4^Act5C.PI show suppression of eye development in the eye disc, whereas clones in the dorsal part of the eye disc that are co-expressing tsh^Scer\UAS.cGa and fng^Scer\UAS.cKa under the control of Scer\GAL4^Act5C.PI induce ectopic eye development.

Co-expression of tsh^Scer\UAS.cGa and CG46146^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PI in clones in the eye results in ventral eye suppression and dorsal eye enlargements.

(Singh et al., 2004)

When tsh^Scer\UAS.cGa (driven by Scer\GAL4^69B) is added to ci⁹⁴ homozygotes, denticles fail to form.

(Angelats et al., 2002)

Clones of cells in the eye disc expressing both hth^{Scer\UAS.T:Avic\GFP} and tsh^Scer\UAS.cGa under the control of Scer\GAL4^αTub84B.PZ grow well in the middle of the disc and can induce large outgrowths at the edge of the disc.

(Bessa et al., 2002)

The split eye phenotype seen in flies expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 is suppressed by hth^dtl-S142204/+ and pupal lethality is suppressed to 16% (from 42%). The frequency of the split eye phenotype seen in flies expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 is reduced to 2% by wg^l-12 (flies shifted to the restrictive temperature 48-72 hours after egg laying) and pupal lethality is suppressed to 19% (from 42%). Coexpression of both hth^Scer\UAS.cPa and tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in complete eye loss and no antennal eye is produced. Coexpression of both arm^{Δ.Scer\UAS.T:Ivir\HA1} and tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI suppresses eye development in both the dorsal and ventral domains and both in marginal and internal regions of the eye disc. Coexpression of both arm^{Δ.Scer\UAS.T:Ivir\HA1} and tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in complete suppression of the eye. Coexpression of both arm^{Δ.Scer\UAS.T:Ivir\HA1} and tsh^Scer\UAS.cGa under the control of Scer\GAL4^bi-omb-Gal4 results in eyes which are reduced in both the dorsal and ventral margins in discs and in adults. Coexpression of both pan^ΔN.Scer\UAS and tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI does not suppress eye development. Coexpression of both pan^ΔN.Scer\UAS and tsh^Scer\UAS.cGa under the control of Scer\GAL4^bi-omb-Gal4 does not result in suppression of eye fate either in the dorsal or ventral margins of the eye. Coexpression of both pan^ΔN.Scer\UAS and tsh^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH does not suppress eye development. Coexpression of both sgg^S9A.Scer\UAS and tsh^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH does not suppress eye development. Coexpression of both sgg^S9A.Scer\UAS and tsh^Scer\UAS.cGa in clones under the control of Scer\GAL4^Act5C.PI does not suppress eye development either in the ventral or dorsal margin of the eye. Coexpression of both sgg^S9A.Scer\UAS and tsh^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 does not suppress eye development either in the ventral or dorsal margin of the eye.

(Singh et al., 2002)

When tsh^Scer\UAS.cGa and arm^{S10.Scer\UAS.T:Hsap\MYC} are coexpressed in clones in under the control of Scer\GAL4^{arm.T:Hsim\VP16}, very few adults survive. Those that do have no clones.

(Erkner et al., 1999)

When expression is driven by Scer\GAL4^69B the ectopic sclerites of spen^poc361 are completely suppressed.

(Wiellette et al., 1999)

The lawn of denticles phenotype of pan³ or wg^l-17 embryos is replaced by naked cuticle if the embryos are also expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^69B. The lawn of denticles phenotype of arm² embryos derived from arm² female germ line clones is partly suppressed if the embryos are also expressing tsh^Scer\UAS.cGa under the control of Scer\GAL4^69B.

(Gallet et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

tsh^UAS.cGa/Scer\GAL4^69B partially rescues tsh⁸

(Laugier et al., 2005)

Comments

Expression of tsh^Scer\UAS.cGa at 22^oC, under the control of Scer\GAL4^69B, rescues the denticle and naked cuticle phenotype of tsh⁸ mutants and Ti segment identity is restored. However, this expression induces ectopic denticle formation in the head region.

(Laugier et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

52216

w^*; P{UAS-tsh.G}2

52217

w^*; P{UAS-tsh.G}3/TM3, Sb¹

Notes on Origin

Discoverer